Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	642,734	G→A	intergenic (+84/‑258)	unknown → / → unknown	Outer membrane protein A precursor/Monothiol glutaredoxin GrxD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	642,734	0	G	A	100.0%	108.4 / NA	41	intergenic (+84/‑258)	unknown/unknown	Outer membrane protein A precursor/Monothiol glutaredoxin GrxD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/17);  total (24/17)

ATCTAAAGGTGTTTCTCAACAAGCCATCTCTGCTACCGGTCACGGTAAAGCAAATCCGGTTACCGGTAACAAATGCGACGTAGTTAAAGGTCGTAAAGCGCTTATCGCTTGTTTGGCTGATGACCGTCGCGTAGAAATCGCTGTTAACGGTACTAAATAATTCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAGTAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATCAGTCAAAATCATGTGCTGAATGAAGCATTACCCCAAACCTTGCCGCCCTAATGGATGAGGCGAGGGCGTTTTGGTAGTAGCCGGTAAATACCCGAGCCAAACATTCAGACCTTTATTGGTGGGGAAAATTGTTTGTGATACAATACGCGCGTTTTTTTC  >  1/642491‑642968                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                           aTCTAAAGGTGTTTCTCAACAAGCCATCTCTGCTACCGGTCACGGTAAAGCAAATCCGGTTACCGGTAACAAATGCGACGTAGTTAAAGGTCGTAAAGCGCTTATCGCTTGTTTGGCTGATGACCGTCGCGTAGAAATCGCTGTTAACGGTACTAAATAATTCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGaaa                                                                                                                                                                                                                                     >  1:594841/1‑251 (MQ=255)            tttCTCAACAAGCCATCTCTGCTACCGGTCACGGTAAAGCAAATCCGGTTACCGGTAACAAATGCGACGTAGTTAAAGGTCGTAAAGCGCTTATCGCTTGTTTGGCTGATGACCGTCGCGTAGAAATCGCTGTTAACGGTACTAAATAATTCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGc                                                                                                                                                                                                                          <  1:626761/251‑1 (MQ=255)               ctcAACAAGCCATCTCTGCTACCGGTCACGGTAAAGCAAATCCGGTTACCGGTAACAAATGCGACGTAGTTAAAGGTCGTAAAGCGCTTATCGCTTGTTTGGCTGATGACCGTCGCGTAGAAATCGCTGTTAACGGTACTAAATAATTCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTa                                                                                                                                                                                                                       >  1:763137/1‑251 (MQ=255)                            ctctGCTACCGGTCACGGTAAAGCAAATCCGGTTACCGGTAACAAATGCGACGTAGTTAAAGGTCGTAAAGCGCTTATCGCTTGTTTGGCTGATGACCGTCGCGTAGAAATCGCTGTTAACGGTACTAAATAATTCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAgt                                                                                                                                                                                                          >  1:791944/1‑251 (MQ=255)                              ctGCTACCGGTCACGGTAAAGCAAATCCGGTTACCGGTAACAAATGCGACGTAGTTAAAGGTCGTAAAGCGCTTATCGCTTGTTTGGCTGATGACCGTCGCGTAGAAATCGCTGTTAACGGTACTAAATAATTCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAgttg                                                                                                                                                                                                        >  1:305862/1‑251 (MQ=255)                                   aCCGGTCACGGTAAAGCAAATCCGGTTACCGGTAACAAATGCGACGTAGTTAAAGGTCGTAAAGCGCTTATCGCTTGTTTGGCTGATGACCGTCGCGTAGAAATCGCTGTTAACGGTACTAAATAATTCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTtgtg                                                                                                                                                                                                   <  1:507356/251‑1 (MQ=255)                                           cGGTAAAGCAAATCCGGTTACCGGTAACAAATGCGACGTAGTTAAAGGTCGTAAAGCGCTTATCGCTTGTTTGGCTGATGACCGTCGCGTAGAAATCGCTGTTAACGGTACTAAATAATTCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTgg                                                                                                                                                                                            >  1:639651/1‑250 (MQ=255)                                                         cGGTTACCGGTAACAAATGCGACGTAGTTAAAGGTCGTAAAGCGCTTATCGCTTGTTTGGCTGATGACCGTCGCGTAGAAATCGCTGTTAACGGTACTAAATAATTCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTc                                                                                                                                                                             <  1:680265/251‑1 (MQ=255)                                                           gTTACCGGTAACAAATGCGACGTAGTTAAAGGTCGTAAAGCGCTTATCGCTTGTTTGGCTGATGACCGTCGCGTAGAAATCGCTGTTAACGGTACTAAATAATTCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTcc                                                                                                                                                                            <  1:650200/250‑1 (MQ=255)                                                               ccGGTAACAAATGCGACGTAGTTAAAGGTCGTAAAGCGCTTATCGCTTGTTTGGCTGATGACCGTCGCGTAGAAATCGCTGTTAACGGTACTAAATAATTCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACt                                                                                                                                                                       <  1:690211/251‑1 (MQ=255)                                                                      cAAATGCGACGTAGTTAAAGGTCGTAAAGCGCTTATCGCTTGTTTGGCTGATGACCGTCGCGTAGAAATCGCTGTTAACGGTACTAAATAATTCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATCa                                                                                                                                                                >  1:126373/1‑251 (MQ=255)                                                                                   gTTAAAGGTCGTAAAGCGCTTATCGCTTGTTTGGCTGATGACCGTCGCGTAGAAATCGCTGTTAACGGTACTAAATAATTCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAGTAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGc                                                                                                                                                                                           >  1:681618/1‑211 (MQ=255)                                                                                   gTTAAAGGTCGTAAAGCGCTTATCGCTTGTTTGGCTGATGACCGTCGCGTAGAAATCGCTGTTAACGGTACTAAATAATTCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTtata                                                                                                                                                                   >  1:490520/1‑235 (MQ=255)                                                                                           tCGTAAAGCGCTTATCGCTTGTTTGGCTGATGACCGTCGCGTAGAAATCGCTGTTAACGGTACTAAATAATTCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATCAGTCAAAATCATGTGCtgaat                                                                                                                                            <  1:222235/250‑1 (MQ=255)                                                                                                    gcTTATCGCTTGTTTGGCTGATGACCGTCGCGTAGAAATCGCTGTTAACGGTACTAAATAATTCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATCAGTCAAAATCATGTGCTGAATGAAGCATTa                                                                                                                                   >  1:191887/1‑250 (MQ=255)                                                                                                         tCGCTTGTTTGGCTGATGACCGTCGCGTAGAAATCGCTGTTAACGGTACTAAATAATTCATTTGTCAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATCAGTCAAAATCATGTGCTGAATGAAGCATTACCCCaa                                                                                                                             >  1:787627/1‑251 (MQ=255)                                                                                                                  tGGCTGATGACCGTCGCGTAGAAATCGCTGTTAACGGTACTAAATAATTCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATc                                                                                                                                                                 >  1:799750/1‑206 (MQ=255)                                                                                                                             cGTCGCGTAGAAATCGCTGTTAACGGTACTAAATAATTCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATCAGTCAAAATCATGTGCTGAATGAAGCATTACCCCAAACCTTGCCGCCCTAATGGAt                                                                                                         <  1:858273/251‑1 (MQ=255)                                                                                                                                       aaaTCGCTGTTAACGGTACTAAATAATTCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATCAGTCAAAATCATGTGCTGAATGAAGCATTACCCCAAACCTTGCCGCCCTAATGGATGAGGCGAggg                                                                                               <  1:475282/251‑1 (MQ=255)                                                                                                                                       aaaTCGCTGTTAACGGTACTAAATAATTCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATCAGTCAAAATCATGTGCTGAATGAAGCATTACCCCAAACCTTGCCGCCCTAATGGATGAGGCGAggg                                                                                               <  1:641820/251‑1 (MQ=255)                                                                                                                                             cTGTTAACGGTACTAAATAATTCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATCAGTCAAAATCATGTGCTGAATGAAGCATTACCCCAAACCTTGCCGCCCTAATGGATGAGGCGAGGGCGtttt                                                                                         >  1:680486/1‑251 (MQ=255)                                                                                                                                                 tAACGGTACTAAATAATTCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATCAGTCAAAATc                                                                                                                                                       >  1:314342/1‑185 (MQ=255)                                                                                                                                                      gTACTAAATAATTCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATCAGTCAAAATCATGTGCTGAATGAAGCATTACCCCAAAc                                                                                                                           <  1:402439/208‑1 (MQ=255)                                                                                                                                                           aaaTAATTCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTtgtg                                                                                                                                                                                                   >  1:420205/1‑131 (MQ=255)                                                                                                                                                           aaaTAATTCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATCAGTCAAAATCATGTGCTGAATGAAGCATTACCCCAAACCTTGCCGCCCTAATg                                                                                                            >  1:239655/1‑218 (MQ=255)                                                                                                                                                           aaaTAATTCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATCAGTCAAAATCATGTGCTGAATGAAGCATTACCCCAAACCTTGCCGCCCTAATGGATGAGGCGAGGGCGTTTTGGTAGTAGCCGGTa                                                                           >  1:314519/1‑251 (MQ=255)                                                                                                                                                                 ttCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATCAGTCAAAATCATGTGCTGAATGAAGCATTACCCCAAACCTTGCCGCCCTAATGGATGAGGCGAGGGCGTTTTGGTAGTAGCCGGTAAATAcc                                                                     <  1:51451/251‑1 (MQ=255)                                                                                                                                                                   cATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATc                                                                                                                                                                 >  1:877649/1‑157 (MQ=255)                                                                                                                                                                                  aGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTatat                                                                                                                                                                  >  1:611350/1‑141 (MQ=255)                                                                                                                                                                                           agaTAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATCAGTCAAAATCATGTGCTGAATGAAGCATTACCCCAAACCTTGCCGCCCTAATGGATGAGGCGAGGGCGTTTTGGTAGTAGCCGGTAAATAc                                                                      >  1:401488/1‑224 (MQ=255)                                                                                                                                                                                           agaTAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATCAGTCAAAATCATGTGCTGAATGAAGCATTACCCCAAACCTTGCCGCCCTAATGGATGAGGCGAGGGCGTTTTGGTAGTAGCCGGTAAATACCCGAGCCAAACATTCAGACCTTTAt                                             <  1:16477/249‑1 (MQ=255)                                                                                                                                                                                               aataaAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATCAGTCAAAATCATGTGCTGAATGAAGCATTACCCCAAACCTTGCCGCCCTAATGGATGa                                                                                                       <  1:512555/187‑1 (MQ=255)                                                                                                                                                                                                ataaAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATCAGTCAAAATCATGTGCTGAATGAAGCATTAc                                                                                                                                  <  1:866445/159‑1 (MQ=255)                                                                                                                                                                                                ataaAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATCAGTCAAAATCATGTGCTGAATGAAGCATTACCCCAAAcc                                                                                                                          <  1:39374/167‑1 (MQ=255)                                                                                                                                                                                                ataaAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATCAGTCAAAATCATGTGCTGAATGAAGCATTACCCCAAAcc                                                                                                                          >  1:385034/1‑167 (MQ=255)                                                                                                                                                                                                ataaAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATCAGTCAAAATCATGTGCTGAATGAAGCATTACCCCAAACCTTGCCGCCCTAATGGATGAGGCGAGGGCGTTTTGGTAGTAGCCGGTAAATACCCGAGCCAAACATTCAGACCTTTATTGGTggg                                      >  1:855385/1‑251 (MQ=255)                                                                                                                                                                                                                          cTTTTTTTATGTAGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATCAGTCAAAATCATGTGCTGAATGAAGCATTACCCCAAACCTTGCCGCCCTAATGGATGAGGCGAGGGCGTTTTGGTAGTAGCCGGTAAATACCCGAGCCAAACATTCAGACCTTTATTGGTGGGGAAAATTGTTTGTGATAc                    >  1:583976/1‑243 (MQ=255)                                                                                                                                                                                                                                    gTAGTCAGATTTGATAATAAGAAATGAAAAAGAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATCAGTCAAAATCATGTGCTGAATGAAGCATTACCCCAAACCTTGCCGCCCTAATGAAGGAGGCGAGGGCGTTTTGGTAGTAGCCGGTAAATACCCGAGCCAAACATTCAGACCTTTATtg                                           <  1:182376/210‑1 (MQ=255)                                                                                                                                                                                                                                      aGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATCAGTCAAAATCATGTGCTGAATGAAGCATTACCCCAAACCTTGCCGCCCTAATGGATGAGGCGAGGGCGTTTTGGTAGTAGCCGGTAAATACCCGAGCCAAACATTCAGACCTTTATtg                                           <  1:652934/208‑1 (MQ=255)                                                                                                                                                                                                                                      aGTCAGATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATCAGTCAAAATCATGTGCTGAATGAAGCATTACCCCAAACCTTGCCGCCCTAATGGATGAGGCGAGGGCGTTTTGGTAGTAGCCGGTAAATACCCGAGCCAAACATTCAGACCTTTATTGGTGGGGAAAATTGTTTGTGATACAATACGCGCGTTTTTTTc  >  1:125846/1‑249 (MQ=255)                                                                                                                                                                                                                                           gATTTGATAATAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATCAGTCAAAATCATGTGCTGAATGAAGCATTACCCCAAACCTTGCCGCCCTAATGGATGAGGCGAGGGCGTTTTGGTAGTAGCCGGTAAATACCCGAGCCAAACATTCAGACCTTTATTGGTGGGGAAAATTGTTTGTGATACAATAcgcg            >  1:139871/1‑234 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                           ATCTAAAGGTGTTTCTCAACAAGCCATCTCTGCTACCGGTCACGGTAAAGCAAATCCGGTTACCGGTAACAAATGCGACGTAGTTAAAGGTCGTAAAGCGCTTATCGCTTGTTTGGCTGATGACCGTCGCGTAGAAATCGCTGTTAACGGTACTAAATAATTCATTTGTTAATTGTTAGCAAATAGAGATAATAAAGACCTAAACGAAAGTTTAGGTCTTTTTTTATGTAGTCAGATTTGATAGTAAGAAATGAAAAATAGCTTATTTTAATCGCAGTTGTTGTGATTGTGGCAGTCACGTTTATTCCAAACTTATATCAGTCAAAATCATGTGCTGAATGAAGCATTACCCCAAACCTTGCCGCCCTAATGGATGAGGCGAGGGCGTTTTGGTAGTAGCCGGTAAATACCCGAGCCAAACATTCAGACCTTTATTGGTGGGGAAAATTGTTTGTGATACAATACGCGCGTTTTTTTC  >  1/642491‑642968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑