Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 61,271 T→C H145R (CAT→CGT)  unknown ← Transcription termination factor Rho

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*161,2710TC100.0% 287.1 / NA 102H145R (CAT→CGT) unknownTranscription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (54/48);  total (54/48)

Only 100 of 102 total aligned reads displayed.

TTCGCCTTTCACGGAACGTTGCATTTCCGTTACCTCTTCCGGACGTTCATCAATCAGCAAGACGATAAGTTCGACATCAGGGTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAATGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTCGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTAAATTGAAACGACGAATTTGGCTCGGGCTGACATAAATATCATCCGGGCCGGCTAAATAGGAACTGTCGTCGGAACG  >  1/61024‑61506
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             
ttCGCCTTTCACGGAACGTTGCATTTCCGTTACCTCTTCCGGACGTTCATCAATCAGCAAGACGATAAGTTCGACATCAGGGTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACggg                                                                                                                                                                                                                                            <  1:121815/251‑1 (MQ=255)
    ccTTTCACGGAACGTTGCATTTCCGTTACCTCTTCCGGACGTTCATCAATCAGCAAGACGATAAGTTCGACATCAGGGTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACggg                                                                                                                                                                                                                                            >  1:676758/1‑247 (MQ=255)
        tCACGGAACGTTGCATTTCCGTTACCTCTTCCGGACGTTCATCAATCAGCAAGACGATAAGTTCGACATCAGGGTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCg                                                                                                                                                                                                                                    <  1:499105/251‑1 (MQ=255)
         cACGGAACGTTGCATTTCCGTTACCTCTTCCGGACGTTCATCAATCAGCAAGACGATAAGTTCGACATCAGGGTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCgt                                                                                                                                                                                                                                   >  1:696141/1‑251 (MQ=255)
             gAACGTTGCATTTCCGTTACCTCTTCCGGACGTTCATCAATCAGCAAGACGATAAGTTCGACATCAGGGTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑Taa                                                                                                                                                                                                                              >  1:821694/1‑251 (MQ=255)
              aaCGTTGCATTTCCGTTACCTCTTCCGGACGTTCATCAATCAGCAAGACGATAAGTTCGACATCAGGGTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAGTCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑Taa                                                                                                                                                                                                                              <  1:259709/250‑1 (MQ=255)
              aaCGTTGCATTTCCGTTACCTCTTCCGGACGTTCATCAATCAGCAAGACGATAAGTTCGACATCAGGGTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑Taa                                                                                                                                                                                                                              <  1:441956/250‑1 (MQ=255)
               aCGTTGCATTTCCGTTACCTCTTCCGGACGTTCATCAATCAGCAAGACGATAAGTTCGACATCAGGGTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCg                                                                                                                                                                                                                                    >  1:660127/1‑244 (MQ=255)
               aCGTTGCATTTCCGTTACCTCTTCCGGACGTTCATCAATCAGCAAGACGATAAGTTCGACATCAGGGTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATg                                                                                                                                                                                                                            >  1:856970/1‑251 (MQ=255)
                  ttGCATTTCCGTTACCTCTTCCGGACGTTCATCAATCAGCAAGACGATAAGTTCGACATCAGGGTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATgggg                                                                                                                                                                                                                         <  1:577083/251‑1 (MQ=255)
                         tCCGTTACCTCTTCCGGACGTTCATCAATCAGCAAGACGATAAGTTCGACATCAGGGTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGtt                                                                                                                                                                                                                  >  1:151808/1‑251 (MQ=255)
                         tCCGTTACCTCTTCCGGACGTTCATCAATCAGCAAGACGATAAGTTCGACATCAGGGTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGtt                                                                                                                                                                                                                  >  1:656979/1‑251 (MQ=255)
                                            gTTCATCAATCAGCAAGACGATAAGTTCGACATCAGGGTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTtg                                                                                                                                                                                                  >  1:390612/1‑248 (MQ=255)
                                             ttCATCAATCAGCAAGACGATAAGTTCGACATCAGGGTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTtgctg                                                                                                                                                                                               >  1:893640/1‑250 (MQ=255)
                                                             aCGATAAGTTCGACATCAGGGTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑tt                                                                                                                                                                              <  1:570517/250‑1 (MQ=255)
                                                                     ttCGACATCAGGGTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTcgtcgt                                                                                                                                                                     >  1:879856/1‑251 (MQ=255)
                                                                         aCATCAGGGTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGa                                                                                                                                                                  >  1:305249/1‑250 (MQ=255)
                                                                              aGGGTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGt                                                                                                                                                            >  1:443659/1‑251 (MQ=255)
                                                                               gggTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATgggg                                                                                                                                                                                                                         <  1:590719/190‑1 (MQ=255)
                                                                               gggTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTgtcg                                                                                                                                                                         <  1:309691/237‑1 (MQ=255)
                                                                               gggTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGt                                                                                                                                                            >  1:176381/1‑250 (MQ=255)
                                                                               gggTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGt                                                                                                                                                            >  1:168473/1‑250 (MQ=255)
                                                                               gggTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGt                                                                                                                                                            >  1:563988/1‑250 (MQ=255)
                                                                               gggTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGt                                                                                                                                                            >  1:307056/1‑250 (MQ=255)
                                                                               gggTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGt                                                                                                                                                            >  1:332369/1‑250 (MQ=255)
                                                                               gggTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGATTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGt                                                                                                                                                            >  1:657586/1‑250 (MQ=255)
                                                                                 gTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑t                                                                                                                                                                               >  1:316721/1‑229 (MQ=255)
                                                                                     ttGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACttt                                                                                                                                                     <  1:668993/251‑1 (MQ=255)
                                                                                          cGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGCGGGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAagg                                                                                                                                                 <  1:152218/251‑1 (MQ=255)
                                                                                          cGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAagg                                                                                                                                                 <  1:493361/250‑1 (MQ=255)
                                                                                          cGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAagg                                                                                                                                                 <  1:823658/250‑1 (MQ=255)
                                                                                          cGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAagg                                                                                                                                                 <  1:520940/250‑1 (MQ=255)
                                                                                          cGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAagg                                                                                                                                                 <  1:555086/250‑1 (MQ=255)
                                                                                          cGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAagg                                                                                                                                                 <  1:794919/250‑1 (MQ=255)
                                                                                          cGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAagg                                                                                                                                                 <  1:626587/250‑1 (MQ=255)
                                                                                          cGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAagg                                                                                                                                                 <  1:6812/250‑1 (MQ=255)
                                                                                          cGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAagg                                                                                                                                                 <  1:260182/250‑1 (MQ=255)
                                                                                          cGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAagg                                                                                                                                                 <  1:164834/250‑1 (MQ=255)
                                                                                           gTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAaggag                                                                                                                                               >  1:354892/1‑251 (MQ=255)
                                                                                            tGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAagg                                                                                                                                                 <  1:643163/248‑1 (MQ=255)
                                                                                              aTACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGAATTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAggg                                                                                                                                             >  1:504486/1‑250 (MQ=255)
                                                                                                aCTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCaa                                                                                                                                          >  1:178035/1‑251 (MQ=255)
                                                                                                   ttGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGt                                                                                                                                       <  1:446861/251‑1 (MQ=255)
                                                                                                     gtgCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGATCCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAg                                                                                                                                     >  1:856315/1‑251 (MQ=255)
                                                                                                     gtgCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGtt                                                                                                                                                                                                                  <  1:153670/175‑1 (MQ=255)
                                                                                                     gtgCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGAc                                                                                                                                                                 <  1:851182/223‑1 (MQ=255)
                                                                                                        cGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGTCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGt                                                                                                                                  <  1:436143/251‑1 (MQ=255)
                                                                                                                      aaCATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTcc                                                                                                                                                                                   >  1:150322/1‑189 (MQ=255)
                                                                                                                       aCATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTaggc                                                                                                                   <  1:345769/251‑1 (MQ=255)
                                                                                                                       aCATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTaggc                                                                                                                   <  1:375064/251‑1 (MQ=255)
                                                                                                                          tGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGt                                                                                                                >  1:766992/1‑251 (MQ=255)
                                                                                                                            gTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCg                                                                                                                                                                                                                                    >  1:805620/1‑135 (MQ=255)
                                                                                                                            gTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAagg                                                                                                                                                 >  1:694191/1‑216 (MQ=255)
                                                                                                                            gTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCtttagg                                                                                                                    <  1:380853/245‑4 (MQ=255)
                                                                                                                                      cTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTccc                                                                                                     <  1:576093/250‑1 (MQ=255)
                                                                                                                                                     cGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGt                                                                                                                                       >  1:805602/1‑201 (MQ=255)
                                                                                                                                                      gATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGtt                                                                                                                                                                                                                  >  1:767011/1‑126 (MQ=255)
                                                                                                                                                      gATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTcc                                                                                        >  1:856411/1‑247 (MQ=255)
                                                                                                                                                       aTCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCtttagg                                                                                                                    >  1:132839/1‑215 (MQ=255)
                                                                                                                                                       aTCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCAGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGt                                                                                   >  1:656527/1‑251 (MQ=255)
                                                                                                                                                             ccACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTaa                                                                             <  1:415847/251‑1 (MQ=255)
                                                                                                                                                             ccACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTaa                                                                             <  1:184254/251‑1 (MQ=255)
                                                                                                                                                                    gACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTc                                                                                                                                                                                    >  1:529522/1‑142 (MQ=255)
                                                                                                                                                                    gACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGt                                                                                                                                       <  1:350857/186‑1 (MQ=255)
                                                                                                                                                                    gACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTaggc                                                                                                                   <  1:202025/206‑1 (MQ=255)
                                                                                                                                                                    gACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTcc                                                                                        >  1:853212/1‑233 (MQ=255)
                                                                                                                                                                    gACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTaa                                                                             >  1:815826/1‑244 (MQ=255)
                                                                                                                                                                    gACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTAAATTGaaa                                                                      >  1:210131/1‑251 (MQ=255)
                                                                                                                                                                    gACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTAAATTGaa                                                                       >  1:428061/1‑250 (MQ=255)
                                                                                                                                                                     aCCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGAttt                                                                                             >  1:296559/1‑227 (MQ=255)
                                                                                                                                                                      ccTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTAAATTGAAacg                                                                    >  1:257608/1‑251 (MQ=255)
                                                                                                                                                                      ccTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAACgggg                                                                                                                                                                                                                         >  1:876095/1‑103 (MQ=255)
                                                                                                                                                                       cTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTAAATTGAAacga                                                                   <  1:198513/251‑1 (MQ=255)
                                                                                                                                                                           aCCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTaggc                                                                                                                   <  1:591711/199‑1 (MQ=255)
                                                                                                                                                                            ccAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATgggg                                                                                                                                                                                                                         >  1:434728/1‑97 (MQ=255)
                                                                                                                                                                                 cGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTAAATTGAAACGACGAATTTGGc                                                         <  1:552273/251‑1 (MQ=255)
                                                                                                                                                                                       ggCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACt                                                                                                                                                                >  1:397855/1‑142 (MQ=255)
                                                                                                                                                                                        gCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTAAATTGAAACGACGAATTTGGCTCGGGc                                                   <  1:616436/250‑1 (MQ=255)
                                                                                                                                                                                             aTCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTaa                                                                             >  1:560553/1‑219 (MQ=255)
                                                                                                                                                                                             aTCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTAAATTGAAACGACGAATTTGGCTCGGGCTGACAt                                             >  1:250849/1‑251 (MQ=255)
                                                                                                                                                                                              tCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTAAATTGAAACGACGAATTTGGCTCGGGCTGACATa                                            >  1:16009/1‑251 (MQ=255)
                                                                                                                                                                                               cTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTAAATTGAAACGACGAATTTGGCTCGGGCTGACATaa                                           >  1:235856/1‑251 (MQ=255)
                                                                                                                                                                                               cTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTAAATTGAAACGACGAATTTGGCTCGGGCTGACATaa                                           >  1:643388/1‑251 (MQ=255)
                                                                                                                                                                                                 aaaaTACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTAAATTGAAACGACGAATTTGGCTCGGGCTGACATAAat                                         <  1:464241/251‑1 (MQ=255)
                                                                                                                                                                                                   aaTACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTAAATTGAAACGACGAATTTGGCTCGGGCTGACATAAatat                                       <  1:763600/251‑1 (MQ=255)
                                                                                                                                                                                                       cGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTAAATTGAAACGACGAATTTGGCTCGGGCTGACATAAATatcatc                                   <  1:55692/251‑1 (MQ=255)
                                                                                                                                                                                                              gTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCg                                                                                                                                                                                                                                    >  1:589771/1‑54 (MQ=255)
                                                                                                                                                                                                              gTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATAc                                                                                                                                                                                       >  1:374355/1‑98 (MQ=255)
                                                                                                                                                                                                                        cGGTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTAAATTGAAACGACGAATTTGGCTCGGGCTGACATAAATATCATCCGGGCCGGCTAAATAg                   <  1:45553/251‑1 (MQ=255)
                                                                                                                                                                                                                          gTAGAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAagg                                                                                                                                                 >  1:248110/1‑123 (MQ=255)
                                                                                                                                                                                                                           ttggaccggtggcgcgtttcctttttAAACGGGGGTTGGCGTGTTAAT‑GGGTTTAGGTTTCGAAAAGGATTTTGGTGCGGGATTCTTCCGGTTTTGT‑GTTGTTGACTTTGTCAACCTTTAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTAAATTGAAACGACGAATTTGGCTCGGGCTGACATAAATATCATCCGGGCCGGCTAAATAGGaa                <  1:266400/227‑1 (MQ=255)
                                                                                                                                                                                                                             gAACCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTAAATTGAAACGACGAATTTGGCTCGGGCTGACATAAATATCATCCGGGCCGGCTAAATAGGaa                >  1:310095/1‑249 (MQ=255)
                                                                                                                                                                                                                              aaCCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTAAATTGAAACGACGAATTTGGCTCGGGCTGACATAAATATCATCCGGGCCGGCTAAATAGGAAc               <  1:152011/249‑1 (MQ=255)
                                                                                                                                                                                                                              aaCCGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTAAATTGAAACGACGAATTTGGCTCGGGCTGACATAAATATCATCCGGGCCGGCTAAATAGGAACTg             <  1:548391/251‑1 (MQ=255)
                                                                                                                                                                                                                                ccGTTGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTAAATTGAAACGACGAATTTGGCTCGGGCTGACATAAATATCATCCGGGCCGGCTAAATAGGaa                >  1:353581/1‑246 (MQ=255)
                                                                                                                                                                                                                                   ttGCCGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTAAATTGAAACGACGAATTTGGCTCGGGCTGACATAAATATCATCCGGGCCGGCTAAATAGGAACTgtcgtc        <  1:713971/251‑1 (MQ=255)
                                                                                                                                                                                                                                      ccGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTAAATTGAAACGACGAATTTGGCTCGGGCTGACATAAATATCATCCGGGCCGGCTAAATAGGAACTGTCGTCGGa     <  1:318861/251‑1 (MQ=255)
                                                                                                                                                                                                                                      ccGCGTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTAAATTGAAACGACGAATTTGGCTCGGGCTGACATAAATATCATCCGGGCCGGCTAAATAGGAACTGTCGTCGGa     <  1:756657/251‑1 (MQ=255)
                                                                                                                                                                                                                                         cgTTCCATTCTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTAAATTGAAACGACGAATTTGGCTCGGGCTGACATAAATATCATCCGGGCCGGCTAAATAGGAACTGTCGTCGGAACg  >  1:853756/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  cTTAAACGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTAGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTAAATTGAAACGACGAATTTGGCTCGGGCTGACATAAATATCATCCGGGCCGGCTAAATAGGAACTGTCGTCGGa     <  1:207576/239‑1 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             
TTCGCCTTTCACGGAACGTTGCATTTCCGTTACCTCTTCCGGACGTTCATCAATCAGCAAGACGATAAGTTCGACATCAGGGTAATTGTGCGTGATACTTTGTGCGATATTTTGCAGCAACATGGTTTTACCCGCTTTTGGCGGCGCCACGATCAAACCACGCTGACCTTTACCAATCGGGGAGGCCAAATCTAAAATACGGGC‑GGTTAAATCTTCGGTAGAACCGTTGCCGCGTTCCATTCTTAAATGGGAGTTGGCGTG‑TAATGGGGTTAAGTTTTCGAAAAGGATTTTGCTGCGGGATACTTCCGG‑TTTGTCGTCGTTGACTTGGTCAACTTTAAGGAGGGCAAAGTAGCGTTCGCCTTCTTTCGGCGGTCGAATTTTCCCTTCGATTTTGTCCCCGGTCTGTAAATTGAAACGACGAATTTGGCTCGGGCTGACATAAATATCATCCGGGCCGGCTAAATAGGAACTGTCGTCGGAACG  >  1/61024‑61506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑