Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	632,728	A→G	K268E (AAA→GAA)	unknown →	Sialic acid utilization regulator, RpiR family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	632,728	0	A	G	100.0%	161.1 / NA	58	K268E (AAA→GAA)	unknown	Sialic acid utilization regulator, RpiR family
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/31);  total (27/31)

CGATGTGGCGATTGGGATCAGCCATTCGGGCTATTCACAGGAAACCGCCCACACGTTAAAAATCGCCAAACAAAACGGGGCGACGACAGTGGCGTTAACGCACAGTTTGCGTTCGCCTATTACCGAACACGCGGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCAAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTATCATTCAACGAAGACGGCTCTGTGAATGAAAAAGGTTTGCGCGAAATTATTCGTTACAACATCGACAAAATGAAAGTGGACGGCTTGTATGTGGGCGGTTCCACCGGTGAAAACTTCATGCTTTCCACG  >  1/632481‑632973                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      cGATGTGGCGATTGGGATCAGCCATTCGGGCTATTCACAGGAAACCGCCCACACGTTAAAAATCGCCAAACAAAACGGGGCGACGACAGTGGCGTTAACGCACAGTTTGCGTTCGCCTATTACCGAACACGCGGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAAc                                                                                                                                                                                                                                                    <  1:144955/251‑1 (MQ=255)   aTGTGGCGATTGGGATCAGCCATTCGGGCTATTCACAGGAAACCGCCCACACGTTAAAAATCGCCAAACAAAACGGGGCGACGACAGTGGCGTTAACGCACAGTTTGCGTTCGCCTATTACCGAACACGCGGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAg                                                                                                                                                                                                                                                  >  1:391871/1‑251 (MQ=255)              gggATCAGCCATTCGGGCTATTCACAGGAAACCGCCCACACGTTAAAAATCGCCAAACAAAACGGGGCGACGACAGTGGCGTTAACGCACAGTTTGCGTTCGCCTATTACCGAACACGCGGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGa                                                                                                                                                                                                                                        >  1:795522/1‑250 (MQ=255)                      ccATTCGGGCTATTCACAGGAAACCGCCCACACGTTAAAAATCGCCAAACAAAACGGGGCGACGACAGTGGCGTTAACGCACAGTTTGCGTTCGCCTATTACCGAACACGCGGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGAc                                                                                                                                                                                                                                               >  1:175521/1‑235 (MQ=255)                             gttttcaccggtggaaCCGCCCACACGTTAAAAATCGCCAAACAAAACGGGGCGACGACAGTGGCGTTAACGCACAGTTTGCGTTCGCCTATTACCGAACACGCGGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGaa                                                                                                                                                                                                                        >  1:526536/15‑251 (MQ=255)                                      caGGAAACCGCCCACACGTTAAAAATCGCCAAACAAAACGGGGCGACGACAGTGGCGTTAACGCACAGTTTGCGTTCGCCTATTACCGAACACGCGGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAg                                                                                                                                                                                                                          <  1:756564/240‑1 (MQ=255)                                              cGCCCACACGTTAAAAATCGCCAAACAAAACGGGGCGACGACAGTGGCGTTAACGCACAGTTTGCGTTCGCCTATTACCGAACACGCGGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTAttt                                                                                                                                                                                                       >  1:71446/1‑251 (MQ=255)                                              cGCCCACACGTTAAAAATCGCCAAACAAAACGGGGCGACGACAGTGGCGTCAACGCACAGTTTGCGTTCGCCTATTACCGAACACGCGGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTACTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTAttt                                                                                                                                                                                                       >  1:664123/1‑251 (MQ=255)                                                    cacGTTAAAAATCGCCAAACAAAACGGGGCGACGACAGTGGCGTTAACGCACAGTTTGCGTTCGCCTATTACCGAACACGCGGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGaa                                                                                                                                                                                                  >  1:732114/1‑250 (MQ=255)                                                              aTCGCCAAACAAAACGGGGCGACGACAGTGGCGTTAACGCACAGTTTGCGTTCGCCTATTACCGAACACGCGGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTaa                                                                                                                                                                                       <  1:580712/251‑1 (MQ=255)                                                                cGCCAAACAAAACGGGGCGACGACAGTGGCGTTAACGCACAGTTTGCGTTCGCCTATTACCGAACACGCGGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAAt                                                                                                                                                                                     <  1:531834/251‑1 (MQ=255)                                                                    aaacaaaACGGGGCGACGACAGTGGCGTTAACGCACAGTTTGCGTTCGCCTATTACCGAACACGCGGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAAt                                                                                                                                                                                     <  1:839444/247‑1 (MQ=255)                                                                     aacaaaACGGGGCGACGACAGTGGCGTTAACGCACAGTTTGCGTTCGCCTATTACCGAACACGCGGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGgag                                                                                                                                                                                <  1:123854/251‑1 (MQ=255)                                                                          aaCGGGGCGACGACAGTGGCGTTAACGCACAGTTTGCGTTCGCCTATTACCGAACACGCGGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACGCTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAAc                                                                                                                                                                           <  1:804414/251‑1 (MQ=255)                                                                              gggCGACGACAGTGGCGTTAACGCACAGTTTGCGTTCGCCTATTACCGAACACGCGGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTAt                                                                                                                                                                        <  1:161756/250‑1 (MQ=255)                                                                                                 aaCGCACAGTTTGCGTTCGCCTATTACCGAACACGCGGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAAttt                                                                                                                                                    >  1:764097/1‑251 (MQ=255)                                                                                                       caGTTTGCGTTCGCCTATTACCGAACACGCGGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGt                                                                                                                                                                                                            <  1:685677/189‑1 (MQ=255)                                                                                                         gTTTGCGTTCGCCTATTACCGAACACGCGGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCa                                                                                                                                            >  1:81287/1‑251 (MQ=255)                                                                                                              cGTTCGCCTATTACCGAACACGCGGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCattat                                                                                                                                        <  1:619447/250‑1 (MQ=255)                                                                                                                   gCCTATTACCGAACACGCGGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGaac                                                                                                                                                                                                 >  1:261342/1‑188 (MQ=255)                                                                                                                    ccTATTACCGAACACGCGGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTAt                                                                                                                                  >  1:768365/1‑250 (MQ=255)                                                                                                                      tATTACCGAACACGCGGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTATCAt                                                                                                                               <  1:618328/251‑1 (MQ=255)                                                                                                                        ttACCGAACACGCGGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTATCATTc                                                                                                                             <  1:135897/251‑1 (MQ=255)                                                                                                                                 acGCGGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTATCATTCAACGAAGAc                                                                                                                    <  1:535286/251‑1 (MQ=255)                                                                                                                                  cgcgGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTAt                                                                                                                                  <  1:451291/236‑1 (MQ=255)                                                                                                                                    cgGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTATCATTCAACGAAGACGGc                                                                                                                 <  1:206505/251‑1 (MQ=255)                                                                                                                                      gATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCattatt                                                                                                                                       <  1:25386/227‑1 (MQ=255)                                                                                                                                      gATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTATCATTCAACGAAGACGGct                                                                                                                >  1:290317/1‑250 (MQ=255)                                                                                                                                            gTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAAt                                                                                                                                                                                     <  1:9068/175‑1 (MQ=255)                                                                                                                                                 ggTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAgg                                                                                                                                                                                  >  1:334979/1‑173 (MQ=255)                                                                                                                                                 ggTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTATCATTCAACGAAGACGGCTCTGTGAATGaaa                                                                                                    >  1:899529/1‑251 (MQ=255)                                                                                                                                                  gTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCAttatta                                                                                                                                      <  1:58551/216‑1 (MQ=255)                                                                                                                                                     aaCGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTATCATTCAACGAAGACGGCTCTGTGAATGAAAAAgg                                                                                                <  1:555028/251‑1 (MQ=255)                                                                                                                                                         gCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTAttt                                                                                                                                                                                                       <  1:229929/144‑1 (MQ=255)                                                                                                                                                         gCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTATCATTCAACGAAGACGGCTCTGTGAATGAAAAAGGTTTg                                                                                            >  1:48718/1‑251 (MQ=255)                                                                                                                                                                         aCTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTATCATTCAACGAAGACGGCTCTGTGAATGAAAAAGGTTTGCGCGAAATTATTCGtt                                                                            >  1:775773/1‑251 (MQ=255)                                                                                                                                                                          cTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAAt                                                                                                                                                                                     <  1:500901/145‑1 (MQ=255)                                                                                                                                                                          cTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTATCATTCAACGAAGACGGCTCTGTGAATGAAAAAGGTTTGCGCGAAATTATTCGTTa                                                                           >  1:110112/1‑251 (MQ=255)                                                                                                                                                                                 gagaTTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTATCATTCAACGAAGACGGCTCTGTGAATGAAAAAGGTTTGCGCGAAATTATTCGTTACAACAt                                                                     <  1:827548/250‑1 (MQ=255)                                                                                                                                                                                    aTTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTATCATTCAACGAAGACGGCTCTGTGAATGAAAAAGGTTTGCGCGAAATTATTCGTTACAACATCGAc                                                                 <  1:626907/251‑1 (MQ=255)                                                                                                                                                                                              tACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTATCATTCAACGAAGACGGCTCTGTGAATGAAAAAGGTTTGCGCGAAATTATTCGTTACAACATCGACAAAATGAAAg                                                       <  1:630244/251‑1 (MQ=255)                                                                                                                                                                                                           cAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTAt                                                                                                                                  <  1:3010/163‑1 (MQ=255)                                                                                                                                                                                                             attattTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTATCATTCAACGAAGACGGCTCTGTGAATGAAAAAGGTTTGCGCGAAATTATTCGTTACAACATCGACAAAATGAAAGTGGACGGCTTGTAtg                                        <  1:684601/251‑1 (MQ=255)                                                                                                                                                                                                             attattTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTATCATTCAACGAAGACGGCTCTGTGAATGAAAAAGGTTTGCGCGAAATTATTCGTTACAACATCGACAAAATGAAAGTGGACGGCTTGTAtg                                        >  1:56453/1‑251 (MQ=255)                                                                                                                                                                                                             attattTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTATCATTCAACGAAGACGGCTCTGTGAATGAAAAAGGTTTGCGCGAAATTATTCGTTACAACATCGACAAAATGAAAGTGGACGGCTTGTAtg                                        >  1:71245/1‑251 (MQ=255)                                                                                                                                                                                                             attattTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTATCATTCAACGAAGACGGCTCTGTGAATGAAAAAGGTTTGCGCGAAATTATTCGTTACAACATCGACAAAATGAAAGTGGACGGCTTGTAtg                                        <  1:676048/251‑1 (MQ=255)                                                                                                                                                                                                              ttattTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTATCATTCAACGAAGACGGCTCTGTGAATGAAAAAGGTTTGCGCGAAATTATTCGTTACAACATCGACAAAATGAAAGTGGACGGCTTGTAtgt                                       <  1:474946/251‑1 (MQ=255)                                                                                                                                                                                                               tattTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAAc                                                                                                                                                                           >  1:725380/1‑118 (MQ=255)                                                                                                                                                                                                                         ggATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGt                                                                                                                                                                                            >  1:529071/1‑91 (MQ=255)                                                                                                                                                                                                                         ggATTTGATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTAt                                                                                                                                  <  1:545694/149‑1 (MQ=255)                                                                                                                                                                                                                               gATCTACGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTa                                                                                                                                   >  1:170230/1‑142 (MQ=255)                                                                                                                                                                                                                                    aCGCTTTATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTATCATTCAACGAAGACGGCTCTGTGAATGAAAAAGGTTTGCGCGAAATTATTCGTTACAACATCGACAAAATGAAAGTGGACGGCTTGTATGTGGGCGGTTCCACCGGTGaaaa                  >  1:187530/1‑250 (MQ=255)                                                                                                                                                                                                                                         ttATTGGTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTATCATTCAACGAAGACGGCTCTGTGAATGAAAAAGGTTTGCGCGAAATTATTCGTTACAACATCGACAAAATGAAAGTGGACGGCTTGTATGTGGGCGGTTCCACCGGTGAAAACTTCAt            >  1:761169/1‑251 (MQ=255)                                                                                                                                                                                                                                              ggTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTaa                                                                                                                                                                                       <  1:186722/75‑1 (MQ=255)                                                                                                                                                                                                                                               gTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTATCATTCAACGAAGACGGCTCTGTGAATGAAAAAGGTTTGCGCGAAATTATTCGTTACAACATCGACAAAATGAAAGTGGACGGCTTGTATGTGGGCGGTTCCACCGGTGAAAACTTCATGc          <  1:573082/247‑1 (MQ=255)                                                                                                                                                                                                                                               gTACAAGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTATCATTCAACGAAGACGGCTCTGTGAATGAAAAAGGTTTGCGCGAAATTATTCGTTACAACATCGACAAAATGAAAGTGGACGGCTTGTATGTGGGCGGTTCCACCGGTGAAAACTTCATGCttt       >  1:525879/1‑250 (MQ=255)                                                                                                                                                                                                                                                   aaGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTATCATTCAACGAAGACGGCTCTGTGAATGAAAAAGGTTTGCGCGAAATTATTCGTTACAACATCGACAAAATGAAAGTGGACGGCTTGTATGTGGGCGGTTCCACCGGTGAAAACTTCATGCTTTCCACg  >  1:326918/1‑251 (MQ=255)                                                                                                                                                                                                                                                    aGCCGAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATg                                                                                                                                                                                                             >  1:688362/1‑47 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      CGATGTGGCGATTGGGATCAGCCATTCGGGCTATTCACAGGAAACCGCCCACACGTTAAAAATCGCCAAACAAAACGGGGCGACGACAGTGGCGTTAACGCACAGTTTGCGTTCGCCTATTACCGAACACGCGGATTTTGTACTGGTCAACGGCAATAAACAAGGGAAACTCCAGGGAGATTCCATCGGTACGAAAATCGCTCAATTATTTGTATTGGATTTGATCTACGCTTTATTGGTACAAGCCAAACAGGACGCCGCGACCAAAACCAAACAGAAAACACTTAATGTTATTTTAGAACAACGTATTAAATAGGAGAAAACTATGCGGGATTTAAAAGGAATTTTCAGTGCATTATTAGTATCATTCAACGAAGACGGCTCTGTGAATGAAAAAGGTTTGCGCGAAATTATTCGTTACAACATCGACAAAATGAAAGTGGACGGCTTGTATGTGGGCGGTTCCACCGGTGAAAACTTCATGCTTTCCACG  >  1/632481‑632973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑