Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	630,219	C→T	intergenic (‑146/‑96)	unknown ← / → unknown	TRAP‑type transport system, periplasmic component, predicted N‑acetylneuraminate‑binding protein/N‑acetylmannosamine‑6‑phosphate 2‑epimerase (EC 5.1.3.9)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	630,219	0	C	T	100.0%	42.9 / NA	18	intergenic (‑146/‑96)	unknown/unknown	TRAP‑type transport system, periplasmic component, predicted N‑acetylneuraminate‑binding protein/N‑acetylmannosamine‑6‑phosphate 2‑epimerase (EC 5.1.3.9)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/8);  total (10/8)

AAATCATATTCTGCGGCATTGGCAACAGAGGTCACACCTAGTGCAATTGCAGTGGCAAGGAAAAGTTTGGCTAGTTTCATGGCTACACTCCTGTAGTAACAAATAATGAATATGGGATGTTTGAATTACAGCTCAGTTTGCATTCTAAAGTAAAAAAATCGTTTTTAAATAGAGAATGAAGCTTTATTTCAAAAATGAGAAGTAGATCACATAGTTGGAATATTACTTCAAAAAATATTTTAAAATGAAATTTTATTTTAGTAGAATGTCTCCATTGACTAGAATGACTGCCAAATGCAGCCGCAAAAAAGAGGAGTTTCTATGTCACAGCTATCTCAAAAATTATCTCATCAAGCCGTTTTCGCCCAACTTCAGAACGGATTAATTGCCTCTTGTCAGCCGGTGGATGATGGTCCGATGGATAAACCGGAAATTGTCGCTGCTATGGCGCAGGCTTCCGT  >  1/629994‑630454                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            aaaTCATATTCTGCGGCATTGGCAACAGAGGTCACACCTAGTGCAATTGCAGTGGCAAGGAAAAGTTTGGCTAGTTTCATGGCTACACTCCTGTAGTAACAAATAATGAATATGGGATGTTTGAATTACAGCTCAGTTTGCATTCTAAAGTAAAAAAATCGTTTTTAAATAGAGAATGAAGCTTTATTTCAAAAATGAGAAGTAGATCACATAGTTGGAATATTATTTCAAAAAATATTTTAAAATGAAat                                                                                                                                                                                                                    >  1:779217/1‑251 (MQ=255)                       cAACAGAGGTCACACCTAGTGCAATTGCAGTGGCAAGGAAAAGTTTGGCTAGTTTCATGGCTACACTCCTGTAGTAACAAATAATGAATATGGGATGTTTGAATTACAGCTCAGTTTGCATTCTAAAGTAAAAAAATCGTTTTTAAATAGAGAATGAAGCTTTATTTCAAAAATGAGAAGTAGATCACATAGTTGGAATATTATTTCAAAAAATATTTTAAAATGAAATTTTATTTTAGTAGAATGtctc                                                                                                                                                                                               >  1:298774/1‑250 (MQ=255)                                   cacCTAGTGCAATTGCAGTGGCAAGGAAAAGTTTGGCTAGTTTCATGGCTACACTCCTGTAGTAACAAATAATGAATATGGGATGTTTGAATTACAGCTCAGTTTGCATTCTAAAGTAAAAAAATCGTTTTTAAATAGAGAATGAAGCTTTATTTCAAAAATGAGAAGTAGATCACATAGTTGGAATATTATTTCAAAAAATATTTTAAAATGAAATTTTATTTTAGTAGAATGTCTCCACTGACTAGAAt                                                                                                                                                                                  <  1:636916/251‑1 (MQ=255)                                                                                        cTCCTGTAGTAACAAATAATGAATATGGGATGTTTGAATTACAGCTCAGTTTGCATTCTAAAGTAAAAAAATCGTTTTTAAATAGAGAATGAAGCTTTATTTCAAAAATGAGAAGTAGATCACATAGTTGGAATATTATTTCAAAAAATATTTTAAAATGAAATTTTATTTTAGTAGAATGTCTCCACTGACt                                                                                                                                                                                       <  1:106523/193‑1 (MQ=255)                                                                                        cTCCTGTAGTAACAAATAATGAATATGGGATGTTTGAATTACAGCTCAGTTTGCATTCTAAAGTAAAAAAATCGTTTTTAAATAGAGAATGAAGCTTTATTTCAAAAATGAGAAGTAGATCACATAGTTGGAATATTATTTCAAAAAATATTTTAAAATGAAATTTTATTTTAGTAGAATGTCTCCACTGACTAGAATGACTGCCAAATGCAGCCGCAAAAAAGAGGAGTTTCTATGTCACAGCTATCTCa                                                                                                                             >  1:71736/1‑251 (MQ=255)                                                                                        cTCCTGTAGTAACAAATAATGAATATGGGATGTTTGAATTACAGCTCAGTTTGCATTCTAAAGTAAAAAAATCGTTTTTAAATAGAGAATGAAGCTTTATTTCAAAAATGAGAAGTAGATCACATAGTTGGAATATTATTACAAAAAATATTTTAAAATGAAATTTTATTTTAGTAGAATGTCTCCACTGACTAGAATGACTGCCAAATGCAGCCGCAAAAAAGAGGAGTTTCTATGTCACAGCTATCTCa                                                                                                                             >  1:237341/1‑251 (MQ=255)                                                                                          ccTGTAGTAACAAATAATGAATATGGGATGTTTGAATTACAGCTCAGTTTGCATTCTAAAGTAAAAAAATCGTTTTTAAATAGAGAATGAAGCTTTATTTCAAAAATGAGAAGTAGATCACATAGTTGGAATATTATTTCAAAAAATATTTTAAAATGAAATTTTATTTTAGTAGAATGTCTCCac                                                                                                                                                                                            <  1:92351/186‑2 (MQ=255)                                                                                                             aaTATGGGATGTTTGAATTACAGCTCAGTTTGCATTCTAAAGTAAAAAAATCGTTTTTAAATAGAGAATGAAGCTTTATTTCAAAAATGAGAAGTAGATCACATAGTTGGAATATTATTTCAAAAAATATTTTAAAATGAAATTTTATTTTAGTAGAATGTCTCCACTGACTAGAATGACTGCCAAATGCAGCCGCAAAAAAgaggag                                                                                                                                                   <  1:56457/208‑1 (MQ=255)                                                                                                               tatGGGATGTTTGAATTACAGCTCAGTTTGCATTCTAAAGTAAAAAAATCGTTTTTAAATAGAGAATGAAGCTTTATTTCAAAAATGAGAAGTAGATCACATAGTTGGAATATTATTTCAAAAAATATTTTAAAATGAAATTTTATTTTAGTAGAATGTCTCCACTGACTAGAATGACTGCCAAATGCAGCCGCAAAAAAGAGGAGTTTCTATGTCACAGCTATCTCAAAAATTATCTCATCAAGCCGttt                                                                                                      >  1:771915/1‑251 (MQ=255)                                                                                                                 tGGGATGTTTGAATTACAGCTCAGTTTGCATTCTAAAGTAAAAAAATCGTTTTTAAATAGAGAATGAAGCTTTATTTCAAAAATGAGAAGTAGATCACATAGTTGGAATATTATTTCAAAAAATATTTTAAAATGAAATTTTATTTTAGTAGAATGTCTCCac                                                                                                                                                                                            <  1:196451/163‑2 (MQ=255)                                                                                                                                          ttGCATTCTAAAGTAAAAAAATCGTTTTTAAATAGAGAATGAAGCTTTATTTCAAAAATGAGAAGTAGATCACATAGTTGGAATATTATTTCAAAAAATATTTTAAAATGAAATTTTATTTTAGTAGAATGTCTCCACTGACTAGAATGACTGCCAAATGCAGCCGCAAAAAAGAGGAGTTTCTATGTCACAGCTATCTCAAAAATTATCTCATCAAGCCGTTTTCGCCCAACTTCAGAACGGATTAATTg                                                                           <  1:794681/251‑1 (MQ=255)                                                                                                                                                                              gaATGAAGCTTTATTTCAAAAATGAGAAGTAGATCACATAGTTGGAATATTATTTCAAAAAATATTTTAAAATGAAATTTTATTTTAGTAGAATGTCTCCACTGACTAGAATGACTGCCAAATGCAGCCGCAAAAAAGAGGAGTTTCTATg                                                                                                                                           >  1:756924/1‑151 (MQ=255)                                                                                                                                                                              gaATGAAGCTTTATTTCAAAAATGAGAAGTAGATCACATAGTTGGAATATTATTTCAAAAAATATTTTAAAATGAAATTTTATTTTAGTAGAATGTCTCCACTGACTAGAATGACTGCCAAATGCAGCCGCAAAAAAGAGGAGTTTCTATGTCACAGCTATCTCAAAAATTATCTCATCAAGcc                                                                                                          >  1:390569/1‑184 (MQ=255)                                                                                                                                                                                     gCTTTATTTCAAAAATGAGAAGTAGATCACATAGTTGGAATATTATTTCAAAAAATATTTTAAAATGAAATTTTATTTTAGTAGAATGTCTCCACTGACTAGAATGACTGCCAAATGCAGCCGCAAAAAAGAGGAGTTTCTATGTCACAGCTATCTCAAAAATTATCTCATCAAGCCGTTTTCGCCCAACTTCAGAACGGATTAATTGCCTCTTGTCAGCCGGTGga                                                        >  1:388346/1‑227 (MQ=255)                                                                                                                                                                                                          gTAGATCACATAGTTGGAATATTATTTCAAAAAATATTTTAAAATGAAATTTTATTTTAGTAGAATGTCTCCACTGACTAGAATGACTGCCAAATGCAGCCGCAAAAAAGAGGAGTTTCTATGTCACAGCTATCTCAAAAATTATCTCATCAAGCCGTTTTCGCCCAACTTCAGAAc                                                                                     <  1:718156/177‑1 (MQ=255)                                                                                                                                                                                                          gTAGATCACATAGTTGGAATATTATTTCAAAAAATATTTTAAAATGAAATTTTATTTTAGTAGAATGTCTCCACTGACTAGAATGACTGCCAAATGCAGCCGCAAAAAAGAGGAGTTTCTATGTCACAGCTATCTCAAAAATTATCTCATCAAGCCGTTTTCGCCCAACTTCAGAACGGATTAATTGCCTCTTGTCAGCCGGTGGATGATGGTCCGATGGATAAACCGGAAATTGTCGCTGCTATGGCGCa           >  1:45375/1‑251 (MQ=255)                                                                                                                                                                                                          gTAGATCACATAGTTGGAATATTATTTCAAAAAATATTTTAAAATGAAATTTTATTTTAGTAGAATGTCTCCACTGACTAGAATGACTGCCAAATGCAGCCGCAAAAAAGAGGAGTTTCTATGTCACAGCTATCTCAAAAATTATCTCATCAAGCCGTTTTCGCCCAACTTCAGAACGGATTAATTGCCTCTTGTCAGCCGGTGGATGATGGTCCGATGGATAAACCGGAAATTGTCGCTGCTATGGCGCa           >  1:387401/1‑251 (MQ=255)                                                                                                                                                                                                                   aTAGTTGGAATATTATTTCAAAAAATATTTTAAAATGAAATTTTATTTTAGTAGAATGTCTCCACTGACTAGAATGACTGCCAAATGCAGCCGCAAAAAAGAGGAGTTTCTATGTCACAGCTATCTCAAAAATTATCTCATCAAGCCGTTTTCGCCCAACTTCAGAACGGATTAATTGCCTCTTGTCAGCCGGTGGATGATGGTCCGATGGATAAACCGGAAATTGTCGCTGCTATGGCGCAGGCTTCCGt  <  1:209759/251‑1 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            AAATCATATTCTGCGGCATTGGCAACAGAGGTCACACCTAGTGCAATTGCAGTGGCAAGGAAAAGTTTGGCTAGTTTCATGGCTACACTCCTGTAGTAACAAATAATGAATATGGGATGTTTGAATTACAGCTCAGTTTGCATTCTAAAGTAAAAAAATCGTTTTTAAATAGAGAATGAAGCTTTATTTCAAAAATGAGAAGTAGATCACATAGTTGGAATATTACTTCAAAAAATATTTTAAAATGAAATTTTATTTTAGTAGAATGTCTCCATTGACTAGAATGACTGCCAAATGCAGCCGCAAAAAAGAGGAGTTTCTATGTCACAGCTATCTCAAAAATTATCTCATCAAGCCGTTTTCGCCCAACTTCAGAACGGATTAATTGCCTCTTGTCAGCCGGTGGATGATGGTCCGATGGATAAACCGGAAATTGTCGCTGCTATGGCGCAGGCTTCCGT  >  1/629994‑630454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑