Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	619,324	C→T	A265V (GCG→GTG)	unknown →	putative membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	619,324	0	C	T	100.0%	212.2 / NA	79	A265V (GCG→GTG)	unknown	putative membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/49);  total (30/49)

TCGGTTCATTGATCGCAGGAAAAAGTGCGGTCAGAATTTCAGGTGTTTTTAACGGGCATACGGAACACCACGAAAATTGCGGTTGCGGGCATCAACATGTGCCGAACCCCGAACGACTCAATCAAAGTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGCGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACTTTATGGCACCACATTGCCGATAAGTGGCGGGGCAGCATTATTTG  >  1/619078‑619554                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       tCGGTTCATTGATCGCAGGAAAAAGTGCGGTCAGAATTTCAGGTGTTTTTAACGGGCATACGGAACACCACGAAAATTGCGGTTGCGGGCATCAACATGTGCCGAACCCCGAACGACTCAATCAAAGTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGac                                                                                                                                                                                                                                     <  1:23722/250‑1 (MQ=255)  cGGTTCATTGATCGCAGGAAAAAGTGCGGTCAGAATTTCAGGTGTTTTTAACGGGCATACGGAACACCACGAAAATTGCGGTTGCGGGCATCAACATGTGCCGAACCCCGAACGACTCAATCAAAGTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGacac                                                                                                                                                                                                                                   <  1:35146/251‑1 (MQ=255)                    aaaaaGTGCGGTCAGAATTTCAGGTGTTTTTAACGGGCATACGGAACACCACGAAAATTGCGGTTGCGGGCATCAACATGTGCCGAACCCCGAACGACTCAATCAAAGTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGc                                                                                                                                                                                                                  <  1:497009/250‑1 (MQ=255)                          tGCGGTCAGAATTTCAGGTGTTTTTAACGGGCATACGGAACACCACGAAAATTGCGGTTGCGGGCATCAACATGTGCCGAACCCCGAACGACTCAATCAAAGTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGaa                                                                                                                                                                                                           <  1:304448/251‑1 (MQ=255)                               tCAGAATTTCAGGTGTTTTTAACGGGCATACGGAACACCACGAAAATTGCGGTTGCGGGCATCAACATGTGCCGAACCCCGAACGACTCAATCAAAGTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACa                                                                                                                                                                                                      <  1:262922/251‑1 (MQ=255)                                      ttCAGGTGTTTTTAACGGGCATACGGAACACCACGAAAATTGCGGTTGCGGGCATCAACATGTGCCGAACCCCGAACGACTCAATCAAAGTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGCCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCgg                                                                                                                                                                                               <  1:352644/251‑1 (MQ=255)                                                       ggCATACGGAACACCACGAAAATTGCGGTTGCGGGCATCAACATGTGCCGAACCCCGAACGACTCAATCAAAGTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTTTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCCCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTgg                                                                                                                                                                                >  1:837178/1‑249 (MQ=255)                                                          aTACGGAACACCACGAAAATTGCGGTTGCGGGCATCAACATGTGCCGAACCCCGAACGACTCAATCAAAGTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATgg                                                                                                                                                                                                                          <  1:618284/204‑1 (MQ=255)                                                          aTACGGAACACCACGAAAATTGCGGTTGCGGGCATCAACATGTGCCGAACCCCGAACGACTCAATCAAAGTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTgcgc                                                                                                                                                                           <  1:187905/251‑1 (MQ=255)                                                           tACGGAACACCACGAAAATTGCGGTTGCGGGCATCAACATGTGCCGAACCCCGAACGACTCAATCAAAGTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTg                                                                                                                                                                                                                                 >  1:279349/1‑196 (MQ=255)                                                               gAACACCACGAAAATTGCGGTTGCGGGCATCAACATGTGCCGAACCCCGAACGACTCAATCAAAGTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATg                                                                                                                                                                       >  1:751013/1‑250 (MQ=255)                                                                aaCACCACGAAAATTGCGGTTGCGGGCATCAACATGTGCCGAACCCCGAACGACTCAATCAAAGTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGcc                                                                                                                                                                     <  1:441858/251‑1 (MQ=255)                                                                      acGAAAATTGCGGTTGCGGGCATCAACATGTGCCGAACCCCGAACGACTCAATCAAAGTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATcc                                                                                                                                                               <  1:269294/251‑1 (MQ=255)                                                                           aaTTGCGGTTGCGGGCATCAACATGTGCCGAACCCCGAACGACTCAATCAAAGTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGcc                                                                                                                                                                     <  1:176156/240‑1 (MQ=255)                                                                                        ggCATCAACATGTGCCGAACCCCGAACGACTCAATCAAAGTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACa                                                                                                                                                                                                      <  1:420714/194‑1 (MQ=255)                                                                                          cATCAACATGTGCCGAACCCCGAACGACTCAATCAAAGTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGgcgc                                                                                                                                            <  1:599195/250‑1 (MQ=255)                                                                                          cATCAACATGTGCCGAACCCCGAACGACTCAATCAAAGTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCa                                                                                                                                           >  1:305386/1‑251 (MQ=255)                                                                                           aTCAACATGTGCCGAACCCCGAACGACTCAATCAAAGTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACa                                                                                                                                                                                                      <  1:384514/191‑1 (MQ=255)                                                                                               aCATGTGCCGAACCCCGAACGACTCAATCAAAGTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTTCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGt                                                                                                                                      >  1:770448/1‑251 (MQ=255)                                                                                                          aCCCCGAACGACTCAATCAAAGTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGGATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCg                                                                                                                           >  1:445884/1‑251 (MQ=255)                                                                                                                aaCGACTCAATCAAAGTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGACTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGcc                                                                                                                     <  1:450465/251‑1 (MQ=255)                                                                                                                         atcaaAGTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGc                                                                                                                                                          <  1:23243/205‑1 (MQ=255)                                                                                                                         atcaaAGTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAAt                                                                                                            >  1:87943/1‑251 (MQ=255)                                                                                                                         atcaaAGTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAAt                                                                                                            <  1:628004/251‑1 (MQ=255)                                                                                                                               gTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACa                                                                                                                                                                                                      <  1:680153/155‑1 (MQ=255)                                                                                                                               gTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGcc                                                                                                                                                                     <  1:568431/188‑1 (MQ=255)                                                                                                                               gTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCg                                                                                                                           <  1:622722/230‑1 (MQ=255)                                                                                                                                tACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGa                                                                                                       <  1:389392/249‑1 (MQ=255)                                                                                                                                       aTCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAAt                                                                                              <  1:865267/251‑1 (MQ=255)                                                                                                                                        tCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGTGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATg                                                                                             <  1:346842/251‑1 (MQ=255)                                                                                                                                         cAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGa                                                                                            <  1:696046/251‑1 (MQ=255)                                                                                                                                         cAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGa                                                                                            <  1:114752/251‑1 (MQ=255)                                                                                                                                                         attattTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGcat                                                                            <  1:315/251‑1 (MQ=255)                                                                                                                                                           tattTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGcatca                                                                          >  1:775540/1‑251 (MQ=255)                                                                                                                                                              tttGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGTGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCatat                                                                       <  1:438456/251‑1 (MQ=255)                                                                                                                                                                   gTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGa                                                                                                       <  1:152705/214‑1 (MQ=255)                                                                                                                                                                      tCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTc                                                               <  1:67047/251‑1 (MQ=255)                                                                                                                                                                      tCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTc                                                               <  1:754417/251‑1 (MQ=255)                                                                                                                                                                           aTGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCgg                                                                               <  1:205441/230‑1 (MQ=255)                                                                                                                                                                                ccccTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGcc                                                                                                                                <  1:588591/176‑1 (MQ=255)                                                                                                                                                                                ccccTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGa                                                     >  1:103281/1‑251 (MQ=255)                                                                                                                                                                                   cTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATcc                                                                                                                                                               >  1:716746/1‑142 (MQ=255)                                                                                                                                                                                     gTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTa                                                >  1:43056/1‑251 (MQ=255)                                                                                                                                                                                       tCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACt                                              <  1:16298/251‑1 (MQ=255)                                                                                                                                                                                         cGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACttt                                            <  1:382022/251‑1 (MQ=255)                                                                                                                                                                                         cGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACttt                                            <  1:56488/251‑1 (MQ=255)                                                                                                                                                                                              cgATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACTTTATGGc                                       <  1:577704/251‑1 (MQ=255)                                                                                                                                                                                                aTTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACTTTATGGca                                      >  1:335162/1‑250 (MQ=255)                                                                                                                                                                                                 ttttCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACTTTATGGctcc                                    <  1:63217/251‑4 (MQ=255)                                                                                                                                                                                                 ttttCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACTTTATGGcacc                                    <  1:240147/251‑1 (MQ=255)                                                                                                                                                                                                   ttCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACttt                                            >  1:649030/1‑241 (MQ=255)                                                                                                                                                                                                           gTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACTTTATGGCACCACATTGCCGa                          >  1:570432/1‑251 (MQ=255)                                                                                                                                                                                                           gTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACTTTATGGCACCACATTGCCGa                          >  1:540430/1‑251 (MQ=255)                                                                                                                                                                                                           gTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGGGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACTTTATGGCACCACATTGCCGa                          >  1:709328/1‑251 (MQ=255)                                                                                                                                                                                                             ttCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTgg                                                                                                                                       <  1:687364/140‑1 (MQ=255)                                                                                                                                                                                                               cTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACTTTATGGCACCACATTGCCGATAAg                      <  1:705575/251‑1 (MQ=255)                                                                                                                                                                                                                 ggCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGcc                                                                                                                                >  1:433783/1‑143 (MQ=255)                                                                                                                                                                                                                     tataTGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACTTTATGGCACCACATTGCCGATAAGTGGCgg                <  1:245790/251‑1 (MQ=255)                                                                                                                                                                                                                      atatGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGaaa                                                                                                                                                   <  1:477403/119‑1 (MQ=255)                                                                                                                                                                                                                      atatGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCg                                                                                                                                  <  1:717133/136‑1 (MQ=255)                                                                                                                                                                                                                      atatGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATAc                                                                                                          <  1:420431/160‑1 (MQ=255)                                                                                                                                                                                                                        atGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGc                                                                                                                                                                                                                  >  1:314767/1‑54 (MQ=255)                                                                                                                                                                                                                        atGTTGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACTTTATGGCACCACATTGCCGATAAGTGGCggggg             >  1:124455/1‑250 (MQ=255)                                                                                                                                                                                                                           ttGGATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACTTTATGGCACCACATTGCCGATAAGTGGCGGGgcag           >  1:793948/1‑250 (MQ=255)                                                                                                                                                                                                                              gATCTCTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACTTTATGGCACCACATTGCCGATAAGTGGCGGGGCAGCat        <  1:501340/250‑1 (MQ=255)                                                                                                                                                                                                                                tctcTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGcc                                                                                                   >  1:870481/1‑157 (MQ=255)                                                                                                                                                                                                                                tctcTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACTTTATGGCACCACAt                                >  1:47143/1‑224 (MQ=255)                                                                                                                                                                                                                                tctcTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACTTTATGGCACCACATTGCCGATAAGTgg                   <  1:649656/237‑1 (MQ=255)                                                                                                                                                                                                                                tctcTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACTTTATGGCACCACATTGCCGATAAGTGGCGGGGGAGCattat     >  1:598081/1‑251 (MQ=255)                                                                                                                                                                                                                                tctcTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACTTTATGGCACCACATTGCCGATAAGTGGCGGGGCAGCattat     >  1:796815/1‑251 (MQ=255)                                                                                                                                                                                                                                tctcTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACTTTATGGCACCACATTGCCGATAAGTGGCGGGGCAGCattat     >  1:847172/1‑251 (MQ=255)                                                                                                                                                                                                                                tctcTATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACTTTATGGCACCACATTGCCGATAAGTGGCGGGGCAGCattat     >  1:91784/1‑251 (MQ=255)                                                                                                                                                                                                                                 ctctATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACTTTATGGCACCACATTGCCGATAAGTGGCGGGGCAGCattatt    >  1:125437/1‑251 (MQ=255)                                                                                                                                                                                                                                  tctATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACTTTATGGCACCACATTGCCGATAAGTGGCGGGGCAGCATTAttt   <  1:681662/251‑1 (MQ=255)                                                                                                                                                                                                                                   ctATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGTTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACTTTATGGCACCACATTGCCGATAAGTGGCGGGGCAGCATTATTTg  <  1:436561/251‑1 (MQ=255)                                                                                                                                                                                                                                   ctATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACTTTATGGCACCACATTGCCGATAAg                      >  1:889838/1‑231 (MQ=255)                                                                                                                                                                                                                                   ctATGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACTTTATGGCACCACATTGCCGATAAGTGGCGGGGCAGCATTATTTg  <  1:740472/251‑1 (MQ=255)                                                                                                                                                                                                                                      tGCTTGGGGCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACTTTATGGCACCACATTGCCGATAAGTGGCGGGGCAGCat        >  1:289317/1‑242 (MQ=255)                                                                                                                                                                                                                                            gggCGTGATAGTGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACTTTATGGCACCACATTGc                             >  1:811190/1‑215 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       TCGGTTCATTGATCGCAGGAAAAAGTGCGGTCAGAATTTCAGGTGTTTTTAACGGGCATACGGAACACCACGAAAATTGCGGTTGCGGGCATCAACATGTGCCGAACCCCGAACGACTCAATCAAAGTACCAACATCAAAGAAAGTTTGCTGATTATTTTGAGTATCGGCATGCGCCCCTGTTCCGGCGCGATTTTCGTACTGTTTCTGGCGTATATGTTGGATCTCTATGCTTGGGGCGTGATAGCGACACTGGCAATGGCTCTCGGCACAGGAATGACATTATCGGCCTTTGCGTTGTTGGTGCGCTATGCCCGATCCACTGCCGTGAAAATGGCGCAGTGGTATCGCCTGCCGTTTGCCAATGTGAATACGGAAGCCTTAATGAAATGCCTTGCCGGCATCATATTGATTTTCTTTGCGATGAGTTTACTTTATGGCACCACATTGCCGATAAGTGGCGGGGCAGCATTATTTG  >  1/619078‑619554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑