Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	618,805	A→G	E92G (GAG→GGG)	unknown →	putative membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	618,805	0	A	G	100.0%	155.2 / NA	57	E92G (GAG→GGG)	unknown	putative membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/32);  total (25/32)

GTCTTAGTTCTCGCTATTATTTATCTTTTCCCCTGGCTGTTTAAGCAGGTTGTCGTGTGGCAGCGGGAATTTAATCAACTGATTTCCGGTTACTTGCACGAAATCAAGCAACAGCCCGTGTATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGAGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATTTTAATTTAAGCCAATTATGGCTGGAGCGAACCGCTTATGCGTTAGTGATTTTATTGGGATTAAATTGGTGTTGGAAAAGCGGGAAAACGCTGTTGAAGCAA  >  1/618567‑619010                                                                                                                                                                                                                                               |                                                                                                                                                                                                              gTCTTAGTTCTCGCTATTATTTATCTTTTCCCCTGGCTGTTTAAGCAGGTTGTCGTGTGGCAGCGGGAATTTAATCAACTGATTTCCGGTTACTTGCACGAAATCAAGCAACAGCCCGTGTATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCa                                                                                                                                                                                                   >  1:725021/1‑251 (MQ=255)   cTTAGTTCTCGCTATTATTTATCTTTTCCCCTGGCTGTTTAAGCAGGTTGTCGTGTGGCAGCGGGAATTTAATCAACTGATTTCCGGTTACTTGCACGAAATCAAGCAACAGCCCGTGTATGTCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCaaa                                                                                                                                                                                                 <  1:765306/251‑1 (MQ=255)   cTTAGTTCTCGCTATTATTTATCTTTTCCCCTGGCTGTTTAAGCAGGTTGTCGTGTGGCAGCGGGAATTTAATCAACTGATTTCCGGTTACTTGCACGAAATCAAGCAACAGCCCGTGTATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCaaa                                                                                                                                                                                                 <  1:162329/251‑1 (MQ=255)    ttAGTTCTCGCTATTATTTATCTTTTCCCCTGGCTGTTTAAGCAGGTTGTCGTGTGGCAGCGGGAATTTAATCAACTGATTTCCGGTTACTTGCACGAAATCAAGCAACAGCCCGTGTATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAAt                                                                                                                                                                                                <  1:697963/251‑1 (MQ=255)    ttAGTTCTCGCTATTATTTATCTTTTCCCCTGGCTGTTTAAGCAGGTTGTCGTGTGGCAGCGGGAATTTAATCAACTGATTTCCGGTTACTTGCACGAAATCAAGCAACAGCCCGTGTATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAAt                                                                                                                                                                                                <  1:275589/251‑1 (MQ=255)    ttAGTTCTCGCTATTATTTATCTTTTCCCCTGGCTGTTTAAGCAGGTTGTCGTGTGGCAGCGGGAATTTAATCAACTGATTTCCGGTTACTTGCACGAAATCAAGCAACAGCCCGTGTATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAAt                                                                                                                                                                                                <  1:198541/251‑1 (MQ=255)     tAGTTCTCGCTATTATTTATCTTTTCCCCTGGCTGTTTAAGCAGGTTGTCGTGTGGCAGCGGGAATTTAATCAACTGATTTCCGGTTACTTGCACGAAATCAAGCAACAGCCCGTGTATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATc                                                                                                                                                                                               <  1:310524/251‑1 (MQ=255)           tcGCTATTATTTATCTTTTCCCCTGGCTGTTTAAGCAGGTTGTCGTGTGGCAGCGGGAATTTAATCAACTGATTTCCGGTTACTTGCACGAAATCAAGCAACAGCCCGTGTATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGtt                                                                                                                                                                                          >  1:197499/1‑250 (MQ=255)                       atCTTTTCCCCTGGCTGTTTAAGCAGGTTGTCGTGTGGCAGCGGGAATTTAATCAACTGATTTCCGGTTACTTGCACGAAATCAAGCAACAGCCCGTGTATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAAt                                                                                                                                                                                                <  1:855497/232‑1 (MQ=255)                        tCTTTTCCCCTGGCTGTTTAAGCAGGTTGTCGTGTGGCAGCGGGAATTTAATCAACTGATTTCCGGTTACTTGCACGAAATCAAGCAACAGCCCGTGTATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTaa                                                                                                                                                                            <  1:590457/251‑1 (MQ=255)                           tttCCCCTGGCTGTTTAAGCAGGTTGTCGTGTGGCAGCGGGAATTTAATCAACTGATTTCCGGTTACTTGCACGAAATCAAGCAACAGCCCGTGTATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGc                                                                                                                                                                         <  1:261231/251‑1 (MQ=255)                             tCCCCTGGCTGTTTAAGCAGGTTGTCGTGTGGCAGCGGGAATTTAATCAACTGATTTCCGGTTACTTGCACGAAATCAAGCAACAGCCCGTGTATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAg                                                                                                                                                                       >  1:805887/1‑251 (MQ=255)                                  tGGCTGTTTAAGCAGGTTGTCGTGTGGCAGCGGGAATTTAATCAACTGATTTCCGGTTACTTGCACGAAATCAAGCAACAGCCCGTGTATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTttcttc                                                                                                                                                                                 <  1:867937/236‑1 (MQ=255)                                       gTTTAAGCAGGTTGTCGTGTGGCAGCGGGAATTTAATCAACTGATTTCCGGTTACTTGCACGAAATCAAGCAACAGCCCGTGTATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGtggtgg                                                                                                                                                             >  1:807930/1‑251 (MQ=255)                                                ggTTGTCGTGTGGCAGCGGGAATTTAATCAACTGATTTCCGGTTACTTGCACGAAATCAAGCAACAGCCCGTGTATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTttcttc                                                                                                                                                                                 >  1:834814/1‑222 (MQ=255)                                                             cAGCGGGAATTTAATCAACTGATTTCCGGTTACTTGCACGAAATCAAGCAACAGCCCGTGTATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCg                                                                                                                                        <  1:316415/250‑1 (MQ=255)                                                                      tttAATCAACTGATTTCCGGTTACTTGCACGAAATCAAGCAACAGCCCGTGTATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCg                                                                                                                                        >  1:518123/1‑241 (MQ=255)                                                                       ttAATCAACTGATTTCCGGTTACTTGCACGAAATCAAGCAACAGCCCGTGTATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTtgctg                                                                                                                             <  1:798751/251‑1 (MQ=255)                                                                              aCTGATTTCCGGTTACTTGCACGAAATCAAGCAACAGCCCGTGTATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAg                                                                                                                       >  1:585149/1‑250 (MQ=255)                                                                                     tCCGGTTACTTGCACGAAATCAAGCAACAGCCCGTGTATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTttcttc                                                                                                               <  1:247003/251‑1 (MQ=255)                                                                                                gCACGAAATCAAGCAACAGCCCGTGTATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATttta                                                                                                    >  1:453489/1‑251 (MQ=255)                                                                                                       aTCAAGCAACAGCCCGTGTATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATTTTAATTTAAg                                                                                             >  1:217327/1‑251 (MQ=255)                                                                                                        tCAAGCAACAGCCCGTGTATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGc                                                                                                                                                                         <  1:352513/174‑1 (MQ=255)                                                                                                                  gCCCGTGTATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATtttaatt                                                                                                 >  1:571304/1‑236 (MQ=255)                                                                                                                   cccGTGTATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATTTTAATTTAAGCCAATTAt                                                                                     <  1:504613/247‑1 (MQ=255)                                                                                                                   cccGTGTATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATTTTAATTTAAGCCAATTATGGc                                                                                  <  1:468686/250‑1 (MQ=255)                                                                                                                      gtgtATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTttcttc                                                                                                               >  1:483419/1‑218 (MQ=255)                                                                                                                      gtgtATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATTTTAATTTAAGCCAATTATGGCt                                                                                 <  1:634856/248‑1 (MQ=255)                                                                                                                      gtgtATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATTTTAATTTAAGCCAATTATGGCTGGa                                                                              >  1:77181/1‑251 (MQ=255)                                                                                                                      gtgtATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATTTTAATTTAAGCCAATTATGGCTGGa                                                                              >  1:423514/1‑251 (MQ=255)                                                                                                                                    ccTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCAACAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATTTTAATTTAAGCCAATTATGGCTGGAGCGAACCGCTTATg                                                                <  1:877518/251‑1 (MQ=255)                                                                                                                                      tgctgATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATTTTAATTTAAGCCAATTATGGCTGGAGCGAACCGCTTATGCg                                                              <  1:460147/251‑1 (MQ=255)                                                                                                                                              gCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATtttaatt                                                                                                 <  1:5195/208‑1 (MQ=255)                                                                                                                                               cTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGc                                                                                                                                                                         >  1:730585/1‑135 (MQ=255)                                                                                                                                                       tttttATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATTTTAATTTAAGCCAATTATGGCTGGAGCGAACCGCTTATGCg                                                              >  1:73507/1‑234 (MQ=255)                                                                                                                                                        ttttATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATTTTAATTTAAGCCAATTATGGCTGGAGCGAACCGCTTATGCGTTAGTGATTTTATTGGGa                                            >  1:787231/1‑251 (MQ=255)                                                                                                                                                              aCGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTTAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATTTTAATTTAAGCCAATTATGGCTGGAGCGAACCGCTTATGCGTTAGTGATTTTATTGGGATTAAAt                                      <  1:288338/251‑1 (MQ=255)                                                                                                                                                                 gtgtgtTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGtt                                                                                                                                   <  1:399201/155‑1 (MQ=255)                                                                                                                                                                 gtgtgtTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTttcttc                                                                                                               >  1:824998/1‑175 (MQ=255)                                                                                                                                                                 gtgtgtTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATtttaattt                                                                                                <  1:171411/190‑1 (MQ=255)                                                                                                                                                                   gtgtTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATTTTAATTTAAGCCAATTATGGCTGGAGCGAACCGCTTATGCGTTAGTGATTTTATTGGGATTAAATTgg                                   <  1:44334/249‑1 (MQ=255)                                                                                                                                                                                  ggTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTttcttc                                                                                                               <  1:480632/158‑1 (MQ=255)                                                                                                                                                                                   gTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTttcttc                                                                                                               >  1:617030/1‑157 (MQ=255)                                                                                                                                                                                   gTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATtttaattt                                                                                                >  1:214675/1‑172 (MQ=255)                                                                                                                                                                                   gTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATTTTAATTTAAGCCAATTATGGCTGGAGCGAACCGCTTATGCGTTAGTGATTTTATTGGGATTAAATTGgtgt                                >  1:545460/1‑236 (MQ=255)                                                                                                                                                                                   gTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATTTTAATTTAAGCCAATTATGGCTGGAGCGAACCGCTTATGCGTTAGTGATTTTATTGGGATTAAATTGGTGTTGGAAAAGCGGaaaa                 >  1:100747/1‑251 (MQ=255)                                                                                                                                                                                   gTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATTTTAATTTAAGCCAATTATGGCTGGAGCGAACCGCTTATGCGTTAGTGATTTTATTGGGATTAAATTGGTGTTGGAAAAGCGGGaaa                 >  1:376367/1‑251 (MQ=255)                                                                                                                                                                                                tAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATTTTAATTTAAGCCAATTATGGCTGGAGCGAACCGCTTATGCGTTAGTGATTTTATTGGGATTAAATTGGTGTTGGAAAAGCGGGAAAACGCTGTTGAAg     <  1:861682/250‑1 (MQ=255)                                                                                                                                                                                                tAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATTTTAATTTAAGCCAATTATGGCTGGAGCGAACCGCTTATGCGTTAGTGATTTTATTGGGATTAAATTGGTGTTGGAAAAGCGGGAAAACGCTGTTGAAGc    <  1:387226/251‑1 (MQ=255)                                                                                                                                                                                                 aaaTTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATTTTAATTTAAGCCAATTATGGCTGGAGCGAACCGCTTATGCGTTAGTGATTTTATTGGGATTAAATTGGTGTTGGAAAAGCGGGAAAACGCTGTTGAAGCa   <  1:736181/251‑1 (MQ=255)                                                                                                                                                                                                   aTTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATTTTAATTTAAGCCAATTATGGCTGGAGCGAACCGCTTATGCGTTAGTGATTTTATTGGGATTAAATTGGTGTTGGAAAAGCGGGAAAACGCTGTTGAAGCaa  >  1:442562/1‑250 (MQ=255)                                                                                                                                                                                                       attattGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATTTTAATTTAAGCCAATTATGGc                                                                                  >  1:560272/1‑166 (MQ=255)                                                                                                                                                                                                                ggTTATTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATTTTAATTTAAGCCAATTATGGCTGGAGc                                                                            <  1:836812/163‑1 (MQ=255)                                                                                                                                                                                                                    aTTTAGCCACCCATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGttt                                                                                                                                  >  1:287164/1‑105 (MQ=255)                                                                                                                                                                                                                             cccATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATTTTAATTTAAGCCAATTATGGCTGGAGCGAACCGCTTATGCGTTAGTGATTTTATTGGGATTAAATTGGTGTTGGAAAAGCGGGAAAACGCTGTTGAAGc    <  1:409599/222‑1 (MQ=255)                                                                                                                                                                                                                               cATCAAACCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATTTTAATTTAAGCCAATTATGGCTGGAGCGAACCGCTTATGCGTTAGTGATTTTATTGGGATTAAATTGGTGTTGGAAAAGCGGGAAAACGCTGTTGAAGCa   <  1:133232/221‑1 (MQ=255)                                                                                                                                                                                                                                    aaCCAAGCTCGGGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATTTTAATTTAAGCCAATTATGGCTGGAGCGAACCGCTTATGCGTTAGTGAt                                                      <  1:343556/165‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                              GTCTTAGTTCTCGCTATTATTTATCTTTTCCCCTGGCTGTTTAAGCAGGTTGTCGTGTGGCAGCGGGAATTTAATCAACTGATTTCCGGTTACTTGCACGAAATCAAGCAACAGCCCGTGTATGCCGGCAGCCTGCTGATTGCTGTTAGTTTTTTATACGGTGTGTTTCATGCGCTGGGTCCGGGACACGGTAAATTTATTATTGCCGGTTATTTAGCCACCCATCAAACCAAGCTCGAGCGCAGTATGCAAATCACGTTCTTTTCTTCTTTAACGCAGGGCGTGGTGGCAATTTCGGCGGTGTCAATCGTGGTTTTGCTGCTTCAGCTTTCTTCCGCTTATTTTAATTTAAGCCAATTATGGCTGGAGCGAACCGCTTATGCGTTAGTGATTTTATTGGGATTAAATTGGTGTTGGAAAAGCGGGAAAACGCTGTTGAAGCAA  >  1/618567‑619010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑