Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	615,629	A→G	K273K (AAA→AAG)	unknown →	putative adhesin/invasin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	615,629	0	A	G	100.0%	170.2 / NA	63	K273K (AAA→AAG)	unknown	putative adhesin/invasin
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/25);  total (38/25)

GCATTAACGACCAAAGTTGATCGTAATACTGCGCGTATTGATCGATTAGATAGCCGAGTCAATGAATTAGACAAAGAAGTAAAAAACGGTTTGGCTTCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAAGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGCTGCATCAATAAAACAAAACCCGTTTTCAATGAAAGCGGGTTTTTTTGTGTTTCGCGATTAACAATTATTTCGCAATGCGTTTATATTTAATTCGGTGCGGTTTAAGGGCTTCAGCACCGAAAGTTTTCTTTTTCCA  >  1/615393‑615873                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     gCATTAACGACCAAAGTTGATCGTAATACTGCGCGTATTGATCGATTAGATAGCCGAGTCAATGAATTAGACAAAGAAGTAAAAAACGGTTTGGCTTCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCagt                                                                                                                                                                                                                                        >  1:330012/1‑251 (MQ=255)  cATTAACGACCAAAGTTGATCGTAATACTGCGCGTATTGATCGATTAGATAGCCGAGTCAATGAATTAGACAAAGAAGTAAAAAACGGTTTGGCTTCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCagta                                                                                                                                                                                                                                       <  1:17650/251‑1 (MQ=255)                    aTCGTAATACTGCGCGTATTGATCGATTAGATAGCCGAGTCAATGAATTAGACAAAGAAGTAAAAAACGGTTTGGCTTCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGTGAGCAGCTGTTGGAGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCAGCGAAGGCCGGAGTGGCAGTAAGTACCAATGGTGGCAg                                                                                                                                                                                                                      >  1:119725/1‑250 (MQ=255)                     tCGTAATACTGCGCGTATTGATCGATTAGATAGCCGAGTCAATGAATTAGACAAAGAAGTAAAAAACGGTTTGGCTTCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAgcg                                                                                                                                                                                                                    >  1:84007/1‑251 (MQ=255)                     tCGTAATACTGCGCGTATTGATCGATTAGATAGCCGAGTCAATGAATTAGACAAAGAAGTAAAAAACGGTTTGGCTTCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAgcg                                                                                                                                                                                                                    >  1:194776/1‑251 (MQ=255)                     tCGTAATACTGCGCGTATTGATCGATTAGATAGCCGAGTCAATGAATTAGACAAAGAAGTAAAAAACGGTTTGGCTTCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAgcg                                                                                                                                                                                                                    >  1:632750/1‑251 (MQ=255)                     tCGTAATACTGCGCGTATTGATCGATTAGATAGCCGAGTCAATGAATTAGACAAAGAAGTAAAAAACGGTTTGGCTTCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGGGGCAGTAAGTACCAATGGTGGCAgcg                                                                                                                                                                                                                    >  1:296853/1‑251 (MQ=255)                     tCGTAATACTGCGCGTATTGATCGATTAGATAGCCGAGTCAATGAATTAGACAAAGAAGTAAAAAACGGTTTGGCTTCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCT‑AAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAgcg                                                                                                                                                                                                                    >  1:328007/1‑250 (MQ=255)                       gTAATACTGCGCGTATTGATCGATTAGATAGCCGAGTCAATGAATTAGACAAAGAAGTAAAAAACGGTTTGGCTTCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCa                                                                                                                                                                                                                  >  1:205038/1‑251 (MQ=255)                               gcgcGTATTGGTCGATTAGATAGCCGAGTCAATGAATTAGACAAAGAAGTAAAAAACGGTTTGGCTTCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGATTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATaa                                                                                                                                                                                                          >  1:431032/1‑251 (MQ=255)                                cgcgTATTGATCGATTAGATAGCCGAGTCAATGAATTAGACAAAGAAGTAAAAAACGGTTTGGCTTCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAAtgg                                                                                                                                                                                                                            >  1:537089/1‑232 (MQ=255)                                cgcgTATTGATCGATTAGATAGCCGAGTCAATGAATTAGACAAAGAAGTAAAAAACGGTTTGGCTTCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAAc                                                                                                                                                                                                         <  1:726510/251‑1 (MQ=255)                                           cGATTAGATAGCCGAGTCAATGAATTAGACAAAGAAGTAAAAAACGGTTTGGCTTCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTaaa                                                                                                                                                                                              >  1:845958/1‑251 (MQ=255)                                             aTTAGATAGCCGAGTCAATGAATTAGACAAAGAAGTAAAAAACGGTTTGGCTTCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAAc                                                                                                                                                                                             <  1:687534/250‑1 (MQ=255)                                             aTTAGATAGCCGAGTCAATGAATTAGACAAAGAAGTAAAAAACGGTTTGGCTTCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGCGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAAc                                                                                                                                                                                                         >  1:374099/1‑238 (MQ=255)                                              ttAGATAGCCGAGTCAATGAATTAGACAAAGAAGTAAAAAACGGTTTGGCTTCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACtt                                                                                                                                                                                           >  1:544747/1‑251 (MQ=255)                                                         aGTCAATGAATTAGACAAAGAAGTAAAAAACGGTTTGGCTTCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTaa                                                                                                                                                                                 <  1:562999/250‑1 (MQ=255)                                                                gAATTAGACAAAGAAGTAAAAAACGGTTTGGCTTCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTa                                                                                                                                                                         >  1:883604/1‑251 (MQ=255)                                                                       aCAAAGAAGTAAAAAACGGTTTGGCTTCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTg                                                                                                                                                                    <  1:114783/249‑1 (MQ=255)                                                                             aagTAAAAAACGGTTTGGCTTCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGa                                                                                                                                                            >  1:119624/1‑251 (MQ=255)                                                                                 aaaaaaCGGTTTGGCTTCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTAc                                                                                                                                                                                                                                  >  1:219554/1‑177 (MQ=255)                                                                                     aaCGGTTTGGCTTCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAgg                                                                                                                                                    <  1:555881/251‑1 (MQ=255)                                                                                     aaCGGTTTGGCTTCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAgg                                                                                                                                                    <  1:854880/251‑1 (MQ=255)                                                                                              gCTTCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAAtgg                                                                                                                                                                                                                            >  1:135686/1‑170 (MQ=255)                                                                                                 tCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAAtgg                                                                                                                                                                                                                            >  1:92624/1‑167 (MQ=255)                                                                                                 tCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGACAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTgg                                                                                                                                           >  1:438765/1‑248 (MQ=255)                                                                                                  cccAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAAc                                                                                                                                                                                                         <  1:804087/185‑1 (MQ=255)                                                                                                          gCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCagtaagt                                                                                                                                                                                                                                    <  1:314599/150‑1 (MQ=255)                                                                                                          gCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGCTGCATCAATaa                                                                                                                               >  1:411950/1‑251 (MQ=255)                                                                                                          gCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGCTGCATCAATaa                                                                                                                               >  1:780483/1‑251 (MQ=255)                                                                                                          gCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGCTGCATCAATaa                                                                                                                               >  1:871047/1‑251 (MQ=255)                                                                                                                   ggCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGc                                                                                                                                          >  1:587643/1‑231 (MQ=255)                                                                                                                        aTTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGc                                                                                                                                                                                                                        <  1:144965/148‑1 (MQ=255)                                                                                                                            cAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGCTGCATCAATAAAACAAAACCCGTTTTCaa                                                                                                             <  1:576263/251‑1 (MQ=255)                                                                                                                                       tGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGCTGCATCAATAAAACAAAACCCGTTTTCAATGAAAGCGGGt                                                                                                  <  1:195842/251‑1 (MQ=255)                                                                                                                                               gCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTg                                                                                                                                                                    <  1:671004/177‑1 (MQ=255)                                                                                                                                                 cTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAgg                                                                                                                                                    >  1:709668/1‑191 (MQ=255)                                                                                                                                                    aaCTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAAt                                                                                                                                                          >  1:299744/1‑182 (MQ=255)                                                                                                                                                           gTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGCTGCATCAATaaaac                                                                                                                            <  1:522971/205‑1 (MQ=255)                                                                                                                                                           gTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGCTGCATCAATAAAACAAAACCCGTTTTCAATGAAAGCGGGTTTTTTTGTGTTTCGCGATTa                                                                              >  1:513636/1‑251 (MQ=255)                                                                                                                                                                 cTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCAc                                                                                                                                                                            >  1:686839/1‑151 (MQ=255)                                                                                                                                                                         ggtTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGCTGCATCAATAAAACAAAACCCGTTTTCAATGAAAGCGGGTTTTTTTGTGTTTCGCGATTAACAATTATTTCGCa                                                                >  1:777127/1‑251 (MQ=255)                                                                                                                                                                          gtTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCagtaag                                                                                                                                                                                                                                     <  1:563492/85‑1 (MQ=255)                                                                                                                                                                          gtTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCAc                                                                                                                                                                            >  1:34201/1‑142 (MQ=255)                                                                                                                                                                           ttATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGCTGCATCAATAAAACAAAACCCGTTTTCAATGAAAGCGGGTTTTTTTGTGTTTCGCGATTAACAATTATTTCGCaa                                                               <  1:503783/250‑1 (MQ=255)                                                                                                                                                                           ttATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGCTGCATCAATAAAACAAAACCCGTTTTCAATGAAAGCGGGTTTTTTTGTGTTTCGCGATTAACAATTATTTCGCAAt                                                              >  1:108095/1‑251 (MQ=255)                                                                                                                                                                                 aTCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGCTGCATCAATAAAACAAAACCCGTTTTc                                                                                                               >  1:740826/1‑196 (MQ=255)                                                                                                                                                                                 aTCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGCTGCATCAATAAAACAAAACCCGTTTTCAATGAAAGCGGGTTTTTTTGTGTTTCGCGATTAACAATTATTTCGCAATGCGttt                                                        >  1:80154/1‑251 (MQ=255)                                                                                                                                                                                     aaaaCAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGCTGCATCAATAAAACAAAACCCGTTTTCAATGAAAGCGGGTTTTTTTGTGTTTCGCGATTAACAATTATTTCGCAATGCGTTTatat                                                    <  1:818754/251‑1 (MQ=255)                                                                                                                                                                                           gCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGCTGCATCAAt                                                                                                                                 >  1:643049/1‑168 (MQ=255)                                                                                                                                                                                           gCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGCTGCATCAATAAAACAAAACCCGTTTTCAATGAAAg                                                                                                       <  1:663449/194‑1 (MQ=255)                                                                                                                                                                                                 gCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGGGTTTTTGGCTGCATCAATAAAACAAAACCCGTTTTCAATGAAAGGGGGTTTTTTTGGGTTTCGCGATTAACAATTATTTCGCAATGCGTTTatat                                                    >  1:322654/1‑239 (MQ=255)                                                                                                                                                                                                        gttCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGCCGGTTTAAACTTTGAGTGGTAATTc                                                                                                                                                                              >  1:884799/1‑110 (MQ=255)                                                                                                                                                                                                            aGGTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGCTGCATCAATAAAACAAAACCCGTTTTCAATGAAAGCGGGTTTTTTTGTGTTTCGCGATTAACAATTATTTCGCAATGCGTTTATATTTAATTCGGTGCGGTTTAAggg                              >  1:327037/1‑250 (MQ=255)                                                                                                                                                                                                             ggTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGc                                                                                                                                          >  1:331886/1‑141 (MQ=255)                                                                                                                                                                                                             ggTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGCTGCATCAATAAAACAAAACCCGTTTTCAATGAAAGCGGGTTTTTTTGTGTTTCGCGATTAACAATTATTTCGCAATGCGTTTatat                                                    >  1:238087/1‑227 (MQ=255)                                                                                                                                                                                                              gTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGCTGCATCAATAAAACAAAACCCGTTTTCAATGAAAGCGGGTTTTTTTGTGTTTCGCGATTAACAATTATTTCGCAATGCGTTTATATTTAATTCGGTGCGGTTTAAggg                              <  1:421064/248‑1 (MQ=255)                                                                                                                                                                                                              gTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGCTGCATCAATAAAACAAAACCCGTTTTCAATGAAAGCGGGTTTTTTTGTGTTTCGCGATTAACAATTATTTCGCAATGCGTTTATATTTAATTCGGTGCGGTTTAAggg                              <  1:473820/248‑1 (MQ=255)                                                                                                                                                                                                              gTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGCTGCATCAATAAAACAAAACCCGTTTTCAATGAAAGCGGGTTTTTTTGTGTTTCGCGATTAACAATTATTTCGCAATGCGTTTATATTTAATTCGGTGCGGTTTAAggg                              <  1:282271/248‑1 (MQ=255)                                                                                                                                                                                                              gTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGCTGCATCAATAAAACAAAACCCGTTTTCAATGAAAGCGGGTTTTTTTGTGTTTCGCGATTAACAATTATTTCGCAATGCGTTTATATTTAATTCGGTGCGGTTTAAggg                              <  1:268707/248‑1 (MQ=255)                                                                                                                                                                                                              gTTATCGTTTCAATCAAAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGCTGCATCAATAAAACAAAACCCGTTTTCAATGAAAGCGGGTTTTTTTGTGTTTCGCGATTAACAATTATTTCGCAATGCGTTTATATTTAATTCGGTGCGGTTTAAGGGc                             <  1:271831/249‑1 (MQ=255)                                                                                                                                                                                                                          atcaaAATGTAGCCGCGAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGCTGCATCAATAAAACAAAACCCGTTTTCAATGAAAGCGGGTTTTTTTGTGTTTCGCGATTAACAATTAttt                                                                    <  1:34465/198‑1 (MQ=255)                                                                                                                                                                                                                                       cgcgAAGGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGCTGCATCAATAAAACAAAACCCGTTTTCAATGAAAGCGGGTTTTTTTGTGTTTCGCGATTAACAATTATTTCGCAATGCGTTTATATTTAATTCGGTGCGGTTTAAGGGCTTCAGCACCGAAAGTTTTCTTTTTCCa  <  1:95876/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     GCATTAACGACCAAAGTTGATCGTAATACTGCGCGTATTGATCGATTAGATAGCCGAGTCAATGAATTAGACAAAGAAGTAAAAAACGGTTTGGCTTCCCAAGCGGCACTAAGCGGCTTATTCCAACCGTATAATGTCGGCAGCCTTAACTTGAGTGCAGCTGTTGGTGGTTATAAATCTAAAACAGCACTAGCGGTTGGTTCAGGTTATCGTTTCAATCAAAATGTAGCCGCGAAAGCCGGTGTGGCAGTAAGTACCAATGGTGGCAGCGCAACCTATAACGTCGGTTTAAACTTTGAGTGGTAATTCACTTAAAGTGCGGTCAGAATTTTAGGTGTTTTTGGCTGCATCAATAAAACAAAACCCGTTTTCAATGAAAGCGGGTTTTTTTGTGTTTCGCGATTAACAATTATTTCGCAATGCGTTTATATTTAATTCGGTGCGGTTTAAGGGCTTCAGCACCGAAAGTTTTCTTTTTCCA  >  1/615393‑615873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑