| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 611,087 | A→G | S244S (TCT→TCC) | unknown ← | DNA topoisomerase IV subunit A (EC 5.99.1.3) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 611,087 | 0 | A | G | 98.8% | 230.4 / NA | 81 | S244S (TCT→TCC) | unknown | DNA topoisomerase IV subunit A (EC 5.99.1.3) |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (43/37); minor base C (1/0); total (44/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCGGTGGTGGCGAATAAATGTGCCATCACGGCGTCAATATCCACCCGATTGGAACGCGGCACCAGCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATAGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATAACATCACCGCCGCGTCGGCGATTTCATTAATGTTGTGCGGTGGAATATCCGTCGCCATCCCCACCGCAATGCCGGTGGTGCCGTTGAGTAAAATGTGCGGTAGGCGCGCCGGTAAA > 1/610841‑611333 | aTCGGTGGTGGCGAATAAATGTGCCATCACGGCGTCAATATCCACCCGATTGGAACGCGGCACCAGCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAAc < 1:243651/251‑1 (MQ=255) cGGTGGTGGCGAATAAATGTGCCATCACGGCGTCAATATCCACCCGATTGGAACGCGGCACCAGCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACcg > 1:701040/1‑251 (MQ=255) ggCGAATAAATGTGCCATCACGGCGTCAATATCCACCCGATTGGAACGCGGCACCAGCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCa > 1:339707/1‑251 (MQ=255) gAATAAATGTGCCATCACGGCGTCAATATCCACCCGATTGGAACGCGGCACCAGCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGtt > 1:566942/1‑251 (MQ=255) aTGTGCCATCACGGCGTCAATATCCACCCGATTGGAACGCGGCACCAGCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATaaa < 1:314976/251‑1 (MQ=255) aTGTGCCATCACGGCGTCAATATCCACCCGATTGGAACGCGGCACCAGCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATaaa < 1:530618/251‑1 (MQ=255) tgCCATCACGGCGTCAATATCCACCCGATTGGAACGCGGCACCAGCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATc < 1:684902/250‑1 (MQ=255) tgCCATCACGGCGTCAATATCCACCCGATTGGAACGCGGCACCAGCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCt < 1:354605/251‑1 (MQ=255) ccATCACGGCGTCAATATCCACCCGATTGGAACGCGGCACCAGCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTc < 1:121499/251‑1 (MQ=255) aCGGCGTCAATATCCACCCGATTGGAACGCGGCACCAGCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAat < 1:105312/251‑1 (MQ=255) cGTCAATATCCACCCGATTGGAACGCGGCACCAGCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAatat > 1:143281/1‑249 (MQ=255) gTCAATATCCACCCGATTGGAACGCGGCACCAGCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATAtctt > 1:37578/1‑251 (MQ=255) gTCAATATCCACCCGATTGGAACGCGGCACCAGCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATAtctt > 1:557427/1‑251 (MQ=255) gTCAATATCCACCCGATTGGAACGCGGCACCAGCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATAtctt > 1:280486/1‑251 (MQ=255) tCAATATCCACCCGATTGGAACGCGGCACCAGCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATAtcttc < 1:398870/251‑1 (MQ=255) atatCCACCCGATTGGAACGCGGCACCAGCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCttt > 1:445925/1‑251 (MQ=255) atatCCACCCGATTGGAACGCGGCACCAGCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCttt > 1:343271/1‑251 (MQ=255) atatCCACCCGATTGGAACGCGGCACCAGCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCttt > 1:611015/1‑251 (MQ=255) ccACCCGATTGGAACGCGGCACCAGCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGc < 1:94306/251‑1 (MQ=255) cccGATTGGAACGCGGCACCAGCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGaa > 1:785222/1‑251 (MQ=255) cGATTGGAACGCGGCACCAGCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGACAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAaat > 1:553315/1‑251 (MQ=255) gCACCAGCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTc > 1:622464/1‑250 (MQ=255) cACCAGCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAAc < 1:137030/191‑1 (MQ=255) gCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAAt > 1:117162/1‑205 (MQ=255) gCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCggg > 1:268681/1‑251 (MQ=255) gCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCggg > 1:102317/1‑251 (MQ=255) ggATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGa > 1:402590/1‑198 (MQ=255) ttCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTa > 1:27888/1‑251 (MQ=255) ttCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTa > 1:319107/1‑251 (MQ=255) ttCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTa > 1:139387/1‑251 (MQ=255) ttCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGACTGATGCGGCAAGGCCCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGGGGCGCGCATTTTAATCGAACCGCGACCCCGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTa > 1:792213/1‑251 (MQ=255) aTCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTta > 1:364449/1‑251 (MQ=255) aTCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTta > 1:122251/1‑251 (MQ=255) gCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGcc > 1:604993/1‑233 (MQ=255) cTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTaataat < 1:378819/251‑1 (MQ=255) ttCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAAtt < 1:634782/251‑1 (MQ=255) caCGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTcc < 1:626934/251‑1 (MQ=255) cGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCAc < 1:578045/230‑1 (MQ=255) cGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCAGGGCCCTGCACTAAGGTTAATAAATCGTCCa > 1:711583/1‑250 (MQ=255) aaTATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCg < 1:813684/206‑1 (MQ=255) atCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAg < 1:49353/251‑1 (MQ=255) tCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGtttt > 1:685324/1‑251 (MQ=255) ccaTCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGa < 1:870863/251‑1 (MQ=255) ccaTCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGa < 1:503239/251‑1 (MQ=255) ccaTCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGa < 1:167802/251‑1 (MQ=255) tttttGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATaa < 1:207285/251‑1 (MQ=255) tttttGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATaa < 1:792291/251‑1 (MQ=255) tttttGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATaa < 1:847962/251‑1 (MQ=255) ttttGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATAAc < 1:893623/251‑1 (MQ=255) gCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATAACATCa > 1:646361/1‑250 (MQ=255) gCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATAACATCa > 1:667868/1‑250 (MQ=255) cATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATaa > 1:131942/1‑240 (MQ=255) gcgcGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTTATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATAACATCACCGCCGCGTCGGCGATTTCATTa > 1:475252/1‑251 (MQ=255) aTGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATAACATCACCGCCGCGTCGGCGATTTCATTAAtgt < 1:334901/250‑1 (MQ=255) gTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATAACATCACCGCCGCGTCGGCGATTTCATTAATGTTGTGCGGTGGAata > 1:471373/1‑251 (MQ=255) gTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATAACATCACCGCCGCGTCGGCGATTTCATTAATGTTGTGCGGTGGAata > 1:596008/1‑251 (MQ=255) tGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATAACATCACCGCCGCGTCGGCGATTTCATTAATGTTGTGCGGTGGAatat < 1:869979/251‑1 (MQ=255) tGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATAACATCACCGCCGCGTCGGCGATTTCATTAATGTTGTGCGGTGGAatat < 1:114900/251‑1 (MQ=255) tGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATAACATCACCGCCGCGTCGGCGATTTCATTAATGTTGTGCGGTGGAatat < 1:496931/251‑1 (MQ=255) gAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGa > 1:41785/1‑192 (MQ=255) gAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCAAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATAACATCACCGCCGCGTCGGCGATTTCATTAATGTTGTGCGGTGGAatat < 1:647863/250‑1 (MQ=255) aaGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATAACATCACCGCCGCGTCGGCGATTTCATTAATGTTGTGCGGTGGAATATc < 1:900642/250‑1 (MQ=255) gcgcTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCAc < 1:186643/140‑1 (MQ=255) tGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTcc > 1:771381/1‑155 (MQ=255) gATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGAccc < 1:643135/61‑1 (MQ=255) gATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATaa < 1:665748/180‑1 (MQ=255) gATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATAACATCACCGCCGCGTCGGCGATTTCATTAATGTTGTGCGGTGGAATATCTGTCGCCATCCCCACCGCAATg > 1:487048/1‑250 (MQ=255) aTAACAATTTCACCGTCTTCTTTCTTCCAGGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTAATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATAACATCGCCGCCGCGTCGGCGATTTCATTAATGTTGTGCGGTGGAATATCTGTCGCCATCCCCACCGCAATGc < 1:171463/250‑1 (MQ=255) tAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCAc > 1:153574/1‑133 (MQ=255) tAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATAACATCACCGCCGCGTCGGCGATTTCATTAATGTTGTGCGGTGGAATATCTGTCGCCATCCCCACCGCAATGCCg > 1:524746/1‑251 (MQ=255) cAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATAACATCACCGCCGCGTCGGCGATTTCATTAATGTTGTGCGGTGGAATATCTGTCGCCATCCCCACCGCAATGCCggtg > 1:703951/1‑251 (MQ=255) gTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATAACATCACCGCCGCGTCGGCGATTTCATTAATGTTGTGCGGTGGAATATctg < 1:776239/215‑3 (MQ=255) gTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATAACATCACCGCCGCGTCGGCGATTTCATTAATGTTGTGCGGTGGAATATCTGTCGCCATCCCCACCGCAATGCCgg > 1:7041/1‑239 (MQ=255) ttcttcCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATAACATCACCGCCGCGTCGGCGATTTCATTAATGTTGTGCGGTGGAATATCTGTCGCCATCCCCACCGCAATGCCGGTGGTGCCGTTGAGTAAAAt < 1:869778/251‑1 (MQ=255) cttcCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATAtctt < 1:175087/62‑1 (MQ=255) cttcCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACgg > 1:785107/1‑116 (MQ=255) cttcCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATAACATCACCGCCGCGTCGGCGATTTCATTAATGTTGTGCGGTGGAATATCTGTCGCCATCCCCACCGCAATGCCGGTGGTGCCGTTGAGTAAAAtgt > 1:712878/1‑251 (MQ=255) tcCAAGTGGCGCGCATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATAACATCACCGCCGCGTCGGCGATTTCATTAATGTTGTGCGGTGGAATATCTGTCGCCATCCCCACCGCAATGCCGGTGGTGCCGTTGAGTAAAATGTGc < 1:583852/251‑1 (MQ=255) cATTTTAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATAACATCACCGCCGCGTCGGCGATTTCATTAATGTTGTGCGGTGGAATATCTGTCGCCATCCCCACCGCAATGCCGGTGGTGCCGTTGAGTAAAATGTGCGGTAGGCGCGCCg > 1:283228/1‑251 (MQ=255) ttttAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATaa > 1:606131/1‑139 (MQ=255) tAATGGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATAACATCACCGCCGCGTCGGCGATTTCATTAATGTTGTGCGGTGGAATATCTGTCGCCATCCCCACCGCAATGCCGGTGGTGCCGTTGAGTAAAATGTGCGGTAGGCGCGCCGGTaaa > 1:587245/1‑251 (MQ=255) | ATCGGTGGTGGCGAATAAATGTGCCATCACGGCGTCAATATCCACCCGATTGGAACGCGGCACCAGCACGATGCGCACCGGATTTTCATGATCCGCTTCATCACGAATATCTTCCACCATCGGCAGTTTTTTGGCATTCATCTGCTCGGCGATTTGCGCGATGACTTTCGACGGTGAAGATTGATGCGGCAAGGCGCTGATAACAATTTCACCGTCTTCTTTCTTCCAAGTGGCGCGCATTTTAATAGAACCGCGACCCAGTTGATAAATCTTCCGAATATCTTCTTTCGGCGAAATAATTTCTGCTTCCGTCGGGAAATCGGGGCCTTGCACTACGGTTAATAATTCGTCCAGCGTAGTTTTCGGATTGTCCAATAACATCACCGCCGCGTCGGCGATTTCATTAATGTTGTGCGGTGGAATATCCGTCGCCATCCCCACCGCAATGCCGGTGGTGCCGTTGAGTAAAATGTGCGGTAGGCGCGCCGGTAAA > 1/610841‑611333 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |