Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	603,438	C→T	S96F (TCC→TTC)	unknown →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	603,438	0	C	T	100.0%	209.3 / NA	74	S96F (TCC→TTC)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/42);  total (32/42)

CGCTTTATTTGGTATAGTCGCAGTAGGCGTTGCTGCCTATCTTTTTTACAGCATCGAGATTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTCCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGCCTCTGCCTTTGCTTATTTACAGCAGCAATCGGGCGTTGAAATTCTCTCAAAATTTCCACCGCACTTTTATTGCGCCGGTTCAAAACCGGAACCCTGGATCCGCATTCCGAAAGTCGGCGCCGCCATGAGTCTGCCTCTGGC  >  1/603193‑603681                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    cGCTTTATTTGGCATAGTCGCAGTAGGCGTTGCTGCCTATCTTTTTTACAGCATCGAGATTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGtt                                                                                                                                                                                                                                                <  1:213600/251‑1 (MQ=255)       aTTTGGCATAGTCGCAGTAGGCGTTGCTGCCTATCTTTTTTACAGCATCGAGATTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCa                                                                                                                                                                                                                                          >  1:412766/1‑251 (MQ=255)       aTTTGGCATAGTCGCAGTAGGCGTTGCTGCCTATCTTTTTTACAGCATCGAGATTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCa                                                                                                                                                                                                                                          >  1:77503/1‑251 (MQ=255)          tggcataGTCGCAGTAGGCGTTGCTGCCTATCTTTTTTACAGCATCGAGATTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAg                                                                                                                                                                                                                                       <  1:860468/247‑1 (MQ=255)           ggcataGTCGCAGTAGGCGTTGCTGCCTATCTTTTTTACAGCATCGAGATTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAgg                                                                                                                                                                                                                                      >  1:643090/4‑251 (MQ=255)               taGTCGCAGTAGGCGTTGCTGCCTATCTTTTTTACAGCATCGAGATTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGgcg                                                                                                                                                                                                                                   >  1:773257/1‑250 (MQ=255)                     cAGTAGGCGTTGCTGCCTATCTTTTTTACAGCATCGAGATTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACttt                                                                                                                                                                                                                            >  1:183310/1‑251 (MQ=255)                     cAGTAGGCGTTGCTGCCTATCTTTTTTACAGCATCGAGATTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACtt                                                                                                                                                                                                                             <  1:22260/250‑1 (MQ=255)                           gCGTTGCTGCCTATCTTTTTTACAGCATCGAGATTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTgtgt                                                                                                                                                                                                                        <  1:588426/249‑1 (MQ=255)                           gCGTTGCTGCCTATCTTTTTTACAGCATCGAGATTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTgtgt                                                                                                                                                                                                                        <  1:410452/249‑1 (MQ=255)                             gTTGCTGCCTATCTTTTTTACAGCATCGAGATTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTtg                                                                                                                                                                                                                      >  1:863939/1‑249 (MQ=255)                                 ctgcCTATCTTTTTTACAGCATCGAGATTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCaa                                                                                                                                                                                                                >  1:316969/1‑251 (MQ=255)                                    ccTATCTTTTTTACAGCATCGAGATTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAagag                                                                                                                                                                                                             <  1:313866/251‑1 (MQ=255)                                    ccTATCTTTTTTACAGCATCGAGATTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAaga                                                                                                                                                                                                              <  1:572316/250‑1 (MQ=255)                                    ccTATCTTTTTTACAGCATCGAGATTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAaga                                                                                                                                                                                                              <  1:551857/250‑1 (MQ=255)                                     cTATCTTTTTTACAGCATCGAGATTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAgg                                                                                                                                                                                                            <  1:410295/251‑1 (MQ=255)                                     cTATCTTTTTTACAGCATCGAGATTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAgg                                                                                                                                                                                                            <  1:839214/251‑1 (MQ=255)                                     cTATCTTTTTTACAGCATCGAGATTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAgg                                                                                                                                                                                                            <  1:633749/251‑1 (MQ=255)                                        tCTTTTTTACAGCATCGAGATTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGgtgt                                                                                                                                                                                                         >  1:65345/1‑251 (MQ=255)                                         cTTTTTTACAGCATCGAGATTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTTGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAgg                                                                                                                                                                                                            <  1:454474/247‑1 (MQ=255)                                         cTTTTTTACAGCATCGAGATTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTtgtg                                                                                                                                                                                                                         <  1:745219/234‑1 (MQ=255)                                         cTTTTTTACAGCATCGAGATTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGtt                                                                                                                                                                                                        >  1:273205/1‑251 (MQ=255)                                             tttACAGCATCGAGATTTTTTTGCCTGTCAAAATCCATTTTTCCGGTGCTTCCACGCTGACTTACCAGCCACTCGCAAAACACGAAGATTTAGCCTGTTACCAAGCGGCGCATAAACCGCAAGAAATGACACAAAATAGATTGCGTTTTCTCGTTTGGAATTTGCACAAAGGGCAGGGTGCCTGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTATTGCTGCAAGAGGTGTTAAAt                                                                                                                                                                                                    <  1:612958/251‑1 (MQ=255)                                             tttACAGCATCGAGATTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAAt                                                                                                                                                                                                    <  1:799389/251‑1 (MQ=255)                                                   gCATCGAGATTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCaa                                                                                                                                                                                              >  1:301692/1‑251 (MQ=255)                                                           aTTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTgg                                                                                                                                                                                       <  1:816347/250‑1 (MQ=255)                                                           aTTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGc                                                                                                                                                                                      <  1:46794/251‑1 (MQ=255)                                                             ttttttGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAAt                                                                                                                                                                                                    <  1:216611/235‑1 (MQ=255)                                                                               ccATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAg                                                                                                                                                                                                                                       <  1:179854/182‑1 (MQ=255)                                                                               ccATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAAt                                                                                                                                                                  >  1:24561/1‑251 (MQ=255)                                                                                       ccGGTGCTTCCAAGCTGACTTACCAACCGATCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGAcc                                                                                                                                                          <  1:300365/251‑1 (MQ=255)                                                                                          gTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCa                                                                                                                                                       >  1:340216/1‑251 (MQ=255)                                                                                            gCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCa                                                                                                                                                       >  1:246609/1‑249 (MQ=255)                                                                                             cTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACtt                                                                                                                                                    >  1:627591/1‑251 (MQ=255)                                                                                                     cTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGcc                                                                                                                                              <  1:821592/249‑1 (MQ=255)                                                                                                     cTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGcc                                                                                                                                              <  1:534686/249‑1 (MQ=255)                                                                                                     cTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGCctc                                                                                                                                            <  1:741870/251‑1 (MQ=255)                                                                                                       gACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTATGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGCct                                                                                                                                             >  1:447499/1‑248 (MQ=255)                                                                                                        aCTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGCCTCTg                                                                                                                                          >  1:276839/1‑250 (MQ=255)                                                                                                         cTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTgtgt                                                                                                                                                                                                                        <  1:274600/171‑1 (MQ=255)                                                                                                         cTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAaga                                                                                                                                                                                                              >  1:77493/1‑181 (MQ=255)                                                                                                         cTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTaaa                                                                                                                                                                                                     <  1:473167/190‑1 (MQ=255)                                                                                                         cTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATAc                                                                                                                                                                                                  <  1:782301/193‑1 (MQ=255)                                                                                                         cTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCg                                                                                                                                                             >  1:526559/1‑230 (MQ=255)                                                                                                         cTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGCCTCTGcc                                                                                                                                        <  1:876363/251‑1 (MQ=255)                                                                                                         cTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGCCTCTGcc                                                                                                                                        <  1:796287/251‑1 (MQ=255)                                                                                                               aaCCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGCCTCTGCCTTTGCt                                                                                                                                  <  1:625246/251‑1 (MQ=255)                                                                                                                        cAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGAc                                                                                                                                                           >  1:767938/1‑217 (MQ=255)                                                                                                                         aaaaCACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGCCTCTGCCTTTGCTTATTTAcagc                                                                                                                        <  1:45204/251‑1 (MQ=255)                                                                                                                                   gATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTgtgt                                                                                                                                                                                                                        <  1:361523/145‑1 (MQ=255)                                                                                                                                   gATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAAt                                                                                                                                                                                                    <  1:553823/165‑1 (MQ=255)                                                                                                                                         aCCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGCCTCTGCCt                                                                                                                                       >  1:771165/1‑220 (MQ=255)                                                                                                                                             gTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATAc                                                                                                                                                                                                  >  1:303551/1‑157 (MQ=255)                                                                                                                                             gTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTc                                                                                                                                                               >  1:602516/1‑192 (MQ=255)                                                                                                                                             gTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGCCTCTGCCTTTGCTTATTTAcagc                                                                                                                        <  1:250551/231‑1 (MQ=255)                                                                                                                                             gTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGCCTCTGCCTTTGCTTATTTACAGCAGCAATCGGGCGTTGAAAtt                                                                                                    >  1:398461/1‑251 (MQ=255)                                                                                                                                              ttACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGCCTCTGCCTTTGCTTATTTACAGCAGCAATCGGGCGTTGAAATtc                                                                                                   <  1:351475/251‑1 (MQ=255)                                                                                                                                                         cgcAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGCCTCTGCCt                                                                                                                                       >  1:116781/1‑204 (MQ=255)                                                                                                                                                             aaaaCCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGCCTCTGCCTTTGCTTATTTACAGCAGCAATCGGGCGTTGAAATTCTCTCAAAATTTCCAc                                                                                    <  1:728712/251‑1 (MQ=255)                                                                                                                                                               aaCCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGCCTCTGCCTTTGc                                                                                                                                   <  1:263079/202‑1 (MQ=255)                                                                                                                                                                        aaaTGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTGTGCCAAAGGGCGCGACTTTgtgt                                                                                                                                                                                                                        <  1:802275/108‑1 (MQ=255)                                                                                                                                                                              cacaAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGCCTCTGCCTTTGCTTATTTACAGCAGCAATCGGGCGTTGAAATTCTCTCAAAATTTCCACCGCACTTTTATTgcgc                                                                    >  1:431936/1‑250 (MQ=255)                                                                                                                                                                                caAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGCCTCTGCCTTTGCTTATTTACAGCAGCAATCGGGCGTTGAAATTCTCTCAAAATTTCCACCGCACTTTTAt                                                                         >  1:824243/1‑243 (MQ=255)                                                                                                                                                                                   aaTAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGCCTCTGCCTTTGCTTATTTACAGCAGCAATCGGGCGTTGAAATTCTCTCAAAATTTCCACCGCACTTTTATTGCGCCGGTTc                                                              <  1:72961/251‑1 (MQ=255)                                                                                                                                                                                           ccGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGcc                                                                                                                                              >  1:3506/1‑163 (MQ=255)                                                                                                                                                                                             gTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGCCTCTGCCTTTGCTTATTTACAGCAGCAATCGGGCGTTGAAATTCTCTCAAAATTTCCACCGCACTTTTATTGCGCCGGTTCAAAACCGGaa                                                    >  1:141798/1‑251 (MQ=255)                                                                                                                                                                                                   tCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGCCTCTGCCTTTGCTTATTTACAGCAGCAATCGGGCGTTGAAATTCTCTCAAAATTTCCACCGCACTTTTATTGCGCCGGTTCAAAACCGGAACCCTgg                                              <  1:476679/251‑1 (MQ=255)                                                                                                                                                                                                    cGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGCCTCTGCCTTTGCTTATTTACAGCAGCAATCGGGCGTTGAAATTCTCTCAAAATTTCCACCGCACTTTTATTGCGCCGGTTCAAAACCGGAACCCTGGa                                             >  1:247855/1‑251 (MQ=255)                                                                                                                                                                                                      tttGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGCCTCTGCCTTTGCTTATTTACAGCAGCAATCGGGCGTTGAAATTCTCTCAAAATTTCCACCGCACTTTTATTGCGCCGGTTCAAAACCGGAACCCTGGAt                                            >  1:498320/1‑250 (MQ=255)                                                                                                                                                                                                         ggAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGCCTCTGCCTTTGCTTATTTACAGCAGCAATCGGGCGTTGAAATTCTCTCAAAATTTCCACCGCACTTTTATTGCGCCGGTTCAAAACCGGAACCCTGGATCCg                                         >  1:838123/1‑250 (MQ=255)                                                                                                                                                                                                                            ggATGCCGGTTGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGCCTCTGCCTTTGCt                                                                                                                                  <  1:386097/142‑1 (MQ=255)                                                                                                                                                                                                                                      tGGCAGCAGGCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGCCTCTGCCTTTGCTTATTTACAGCAGCAATCGGGCGTTGAAATTCTCTCAAAATTTCCACCGCACTTTTATTGCGCCGGTTCAAAACCGGAACCCTGGATCCGCATTCCGAAAGTCGGTGCTGCCATGAGTCt           <  1:854994/251‑1 (MQ=255)                                                                                                                                                                                                                                              ggCGCTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGCCTCTGCCTTTGCTTATTTACAGCAGCAATCGGGCGTTGAAATTCTCTCAAAATTTCCACCGCACTTTTATTGCGCCGGTTc                                                              >  1:335057/1‑192 (MQ=255)                                                                                                                                                                                                                                                cgcTTTTCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATCCAGCGCAATTTCCGACCGCACTTTATGCCTCTGCCTTTGCTTATTTACAGCAGCAATCGGGCGTTGAAATTCTCTCAAAATTTCCACCGCACTTTTATTGCGCCGGTTCAAAACCGGAACCCTGGATCCGCATTCCGAAAGTCGGTGCTGCCATGAGTCTGCCTCTGGc  <  1:441764/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    CGCTTTATTTGGTATAGTCGCAGTAGGCGTTGCTGCCTATCTTTTTTACAGCATCGAGATTTTTTTGCCGGTCAAAATCCATTTTTCCGGTGCTTCCAAGCTGACTTACCAACCGCTCGCAAAACACGAAGATTTAACCTGTTACCAAGCGGCGCAAAAACCGCAAGAAATGACACAAAATAGATTCCGTTTACTCGTTTGGAATTTGCACAAAGGGCAGGATGCCGGTTGGCAGCAGGCGCTTTCCCGTTTTGCCAAAGGGCGCGACTTTGTGTTGCTGCAAGAGGTGTTAAATACGCAACATCTGGCGACGCAATATTCAGCGCAATTTCCGACCGCACTTTATGCCTCTGCCTTTGCTTATTTACAGCAGCAATCGGGCGTTGAAATTCTCTCAAAATTTCCACCGCACTTTTATTGCGCCGGTTCAAAACCGGAACCCTGGATCCGCATTCCGAAAGTCGGCGCCGCCATGAGTCTGCCTCTGGC  >  1/603193‑603681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑