Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	601,984	T→C	V293A (GTC→GCC)	unknown →	DNA polymerase III subunits gamma and tau (EC 2.7.7.7)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	601,984	0	T	C	100.0%	255.2 / NA	97	V293A (GTC→GCC)	unknown	DNA polymerase III subunits gamma and tau (EC 2.7.7.7)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (48/49);  total (48/49)

TTCGCGACAGTTTAAGCCTCACCGATCAAGCGATAGCCATGAGCAACGGCAATGTTACGCTCAATATCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGTCGGAAAACTTGCACAAAATCGTCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGGTGTTATTGCGTGCGTTGGCGTTTCACCCGAAATTAATGGGGGATTTTGCGAATGCAGAACAGAACGAATTCAATCCTCCG  >  1/601747‑602228                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                       ttCGCGACAGTTTAAGCCTCACCGATCAAGCGATAGCCATGAGCAACGGCAATGTTACGCTCAATATCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAActc                                                                                                                                                                                                                                           <  1:331240/251‑1 (MQ=255)          gTTTAAGCCTCACCGATCAAGCGATAGCCATGAGCAACGGCAATGTTACGCTCAATATCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAg                                                                                                                                                                                                                                 >  1:746240/1‑251 (MQ=255)          gTTTAAGCCTCACCGATCAAGCGATAGCCATGAGCAACGGCAATGTTACGCTCAATATCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAg                                                                                                                                                                                                                                 >  1:392033/1‑251 (MQ=255)          gTTTAAGCCTCACCGATCAAGCGATAGCCATGAGCAACGGCAATGTTACGCTCAATATCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACGTGCACAAAATCGCCACGGAACAACTCTT‑TCCGCAg                                                                                                                                                                                                                                 >  1:499610/1‑251 (MQ=255)            ttAAGCCTCACCGATCAAGCGATAGCCATGAGCAACGGCAATGTTACGCTCAATATCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGc                                                                                                                                                                                                                               <  1:494963/251‑1 (MQ=255)                  cTCACCGATCAAGCGATAGCCATGAGCAACGGCAATGTTACGCTCAATATCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGGGGCGGAAAAATTGCACAAAAACGCCATGCAAAAAATCTT‑GCCGCAGGCAGCCtt                                                                                                                                                                                                                         >  1:719873/1‑251 (MQ=255)                   tCACCGATCAAGCGATAGCCATGAGCAACGGCAATGTTACGCTCAATATCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTg                                                                                                                                                                                                                        >  1:408139/1‑251 (MQ=255)                   tCACCGATCAAGCGATAGCCATGAGCAACGGCAATGTTACGCTCAATATCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGGGAAGGGGCGGGAAACATGCACAAAATCGCCATGCAAAAAATCTT‑GCCGCAGGCTGCCTTg                                                                                                                                                                                                                        >  1:80672/1‑251 (MQ=255)                            aaGCGATAGCCATGAGCAACGGCAATGTTACGCTCAATATCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGc                                                                                                                                                                                                               <  1:812027/251‑1 (MQ=255)                                     ccATGAGCAACGGCAATGTTACGCTCAATATCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGGCGGCGATTGGGATGAATTGCTACGGGAAGTGGCGGGAAACTTGCACAAAAACGCCCTGCAACAACCCTT‑GCCGCAGGCCGCCTTGGATGAAAGCAGGCaaaa                                                                                                                                                                                                       >  1:783049/1‑249 (MQ=255)                                         gAGCAACGGCAATGTTACGCTCAATATCGTTGACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCgg                                                                                                                                                                                                    >  1:633061/1‑249 (MQ=255)                                              aCGGCAATGTTACGCTCAATATCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑TCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCtttttt                                                                                                                                                                                             >  1:231616/1‑250 (MQ=255)                                              aCGGCAATGTTACGCTCAATATCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAAATGCTACGTGAAGTGGCGGAAAAATTGGACAAAAACGCCATGCAAAAAATCTT‑GCCGCAGGGTGCCTTGGGTGAAAGCAATCAAAACGGGtttttt                                                                                                                                                                                             >  1:11601/1‑250 (MQ=255)                                               cGGCAATGTTACGCTCAATATCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCttttttg                                                                                                                                                                                            >  1:567061/1‑249 (MQ=255)                                                    aTGTTACGCTCAATATCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCCGGCCGCCCTTGATGAAAGCAGTCAAATCGGCTTTTTAG‑cccaa                                                                                                                                                                                      >  1:616209/1‑248 (MQ=255)                                                       ttACGCTCAATATCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACat                                                                                                                                                                                   <  1:422058/251‑1 (MQ=255)                                                       ttACGCTCAATATCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACat                                                                                                                                                                                   <  1:391334/251‑1 (MQ=255)                                                           gCTCAATATCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAAATTGCACAAAAACGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACat                                                                                                                                                                                   >  1:1044/1‑247 (MQ=255)                                                            cTCAATATCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTg                                                                                                                                                                               <  1:790121/250‑1 (MQ=255)                                                            cTCAATATCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTg                                                                                                                                                                               <  1:770981/250‑1 (MQ=255)                                                            cTCAATATCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAAATTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTgg                                                                                                                                                                              >  1:725691/1‑250 (MQ=255)                                                              cAATATCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCCCAAAAACGCCATGCAAAAACTCTT‑TCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCCAACATATTgcg                                                                                                                                                                             >  1:207754/1‑250 (MQ=255)                                                              cAATATCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCCTGCAACAACTCTT‑GCCGCAGGCTGCCCTGGATTGAAGCAGGCAAAACGGCTTTTTAG‑CCAAACATAtttcg                                                                                                                                                                             >  1:894622/1‑247 (MQ=255)                                                              cAATATCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAActc                                                                                                                                                                                                                                           <  1:536539/190‑1 (MQ=255)                                                              cAATATCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGGCAAAACGGCTTTTTAG‑CCAAACATATTgcg                                                                                                                                                                             >  1:866950/1‑250 (MQ=255)                                                                  atCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCaacaac                                                                                                                                                                                                                                             >  1:886775/1‑184 (MQ=255)                                                                  atCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAAATCTT‑GCCGCAGGCTGCCTTGGGTGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCa                                                                                                                                                                        >  1:769858/1‑251 (MQ=255)                                                                     gTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGAt                                                                                                                                                                                                                     <  1:181777/204‑1 (MQ=255)                                                                      ttAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGa                                                                                                                                                                     <  1:194066/250‑1 (MQ=255)                                                                      ttAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGAc                                                                                                                                                                    <  1:171841/251‑1 (MQ=255)                                                                      ttAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGAc                                                                                                                                                                    <  1:654346/251‑1 (MQ=255)                                                                      ttAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGAc                                                                                                                                                                    <  1:10533/251‑1 (MQ=255)                                                                          cGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGtt                                                                                                                                                                 >  1:743594/1‑250 (MQ=255)                                                                                gCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTc                                                                                                                                                                <  1:646040/245‑1 (MQ=255)                                                                                   cGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTTG‑CCAAACATATTGCGCCTCAGGACGGTCAAttttt                                                                                                                                                         >  1:154659/1‑249 (MQ=255)                                                                                     gTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGGATTGCTACGTGAAGTGGCGGAAAACTTGCCCAAAATCGCCCTGCAACAACTCTT‑GCCGCAGGCTGCCTTGGGTGAAAGCAGTCAAATTGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTTAATTTTTCTa                                                                                                                                                      >  1:406041/1‑250 (MQ=255)                                                                                     gTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCAAGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGGCAAATCGGCTTTTTAGCCCAAAC‑TATTGCGCCTCAGGACGGTCAATTTTTCTa                                                                                                                                                      >  1:859004/1‑250 (MQ=255)                                                                                            ggATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAActc                                                                                                                                                                                                                                           <  1:276604/160‑1 (MQ=255)                                                                                            ggATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAg                                                                                                                                                                                                                <  1:452128/186‑1 (MQ=255)                                                                                            ggATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTTG‑CCAAACATATTGCGCCTCCGGACGGTCAAttt                                                                                                                                                           >  1:204447/1‑238 (MQ=255)                                                                                                 aaaGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTc                                                                                                                                         <  1:647531/251‑1 (MQ=255)                                                                                                 aaaGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTc                                                                                                                                         <  1:172282/251‑1 (MQ=255)                                                                                                       aaGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGa                                                                                                                                                                     <  1:894313/217‑1 (MQ=255)                                                                                                         gCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCCCCGGACGTTCAATTTTTCTACCAGGTCGTTGTCAc                                                                                                                                       >  1:717238/1‑245 (MQ=255)                                                                                                          ccATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAAACGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGGTCAATTTTTCTACCAGGTCGTTGTCACCGGTCgg                                                                                                                                >  1:690106/1‑250 (MQ=255)                                                                                                          ccATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGGCAAAACGGCTTTTTAA‑CCAAACATATTGCGCCTCAGGAc                                                                                                                                                                    >  1:237260/1‑215 (MQ=255)                                                                                                              tGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAg                                                                                                                            <  1:567710/251‑1 (MQ=255)                                                                                                              tGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAg                                                                                                                            <  1:839149/251‑1 (MQ=255)                                                                                                              tGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAg                                                                                                                            <  1:192504/251‑1 (MQ=255)                                                                                                               gagaTGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGAc                                                                                                                                                                    <  1:681447/210‑1 (MQ=255)                                                                                                                    gatTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTTCGCCTCAGGAc                                                                                                                                                                    >  1:843090/1‑205 (MQ=255)                                                                                                                    gatTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGGGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCAT‑GCCGCAGGCTGACTTGGATGAAAGCAGGCAAATTGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGGAGTTCAATTTTTTTAACAGGTCGTTGTCCCCGGGCGTAAAGAAATaa                                                                                                                      >  1:607395/1‑251 (MQ=255)                                                                                                                              ttACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAATATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCAc                                                                                                             <  1:347041/250‑1 (MQ=255)                                                                                                                                 cAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCaa                                                                                                          >  1:64606/1‑250 (MQ=255)                                                                                                                                 cAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGAAAAAGCCGGGGATGGGGAGGAATTGTAACGGGAGGGGGGGGAAAATTTGCAAAAAATGGCCAGGAAAAAAC‑CTTGGCCGCAGGCTGCCTTGGAGGAAAGAAGTAAAATCGGCTTTTTAG‑CCAAACAATTTGCGCCCCAGGCGGTTCAATTTTTTTACCAGGTGTTTTTAACCGGTGGTAAAAAATTAAGGGcccaaccaag                                                                                                         >  1:190225/1‑243 (MQ=255)                                                                                                                                   gCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACgg                                                                                                       >  1:398884/1‑251 (MQ=255)                                                                                                                                    cAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACgg                                                                                                       <  1:896224/250‑1 (MQ=255)                                                                                                                                      aGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTAcc                                                                                                                                                    <  1:237925/203‑1 (MQ=255)                                                                                                                                           aCGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATg                                                                                               <  1:215505/251‑1 (MQ=255)                                                                                                                                           aCGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATg                                                                                               <  1:390936/251‑1 (MQ=255)                                                                                                                                              gCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATggg                                                                                             <  1:665461/250‑1 (MQ=255)                                                                                                                                              gCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATggg                                                                                             >  1:155056/1‑250 (MQ=255)                                                                                                                                                      cTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTTTACCAGGTCGTTTTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGGATGGGCGCTGaaaa                                                                                    >  1:102292/1‑250 (MQ=255)                                                                                                                                                        gATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTg                                                                                                                                                                                                                        <  1:436690/118‑1 (MQ=255)                                                                                                                                                              ggcagTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACgg                                                                                                       >  1:36710/1‑224 (MQ=255)                                                                                                                                                               gcagTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGGTGTTAtt                                                                           >  1:556730/1‑251 (MQ=255)                                                                                                                                                                 agTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGt                                                                                                                    <  1:99113/208‑1 (MQ=255)                                                                                                                                                                  gTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGAc                                                                                                                                                                    >  1:554414/1‑159 (MQ=255)                                                                                                                                                                  gTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCAc                                                                                                                                       >  1:660328/1‑188 (MQ=255)                                                                                                                                                                  gTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGGTGTTATtg                                                                          <  1:372745/249‑1 (MQ=255)                                                                                                                                                                  gTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGGTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGGGGTTATtgcg                                                                        >  1:767838/1‑251 (MQ=255)                                                                                                                                                                   tGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGGTGTTATtg                                                                          <  1:512795/248‑1 (MQ=255)                                                                                                                                                                   tGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGGTGTTATtg                                                                          <  1:120969/248‑1 (MQ=255)                                                                                                                                                                   tGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGGTGTTATtg                                                                          <  1:227618/248‑1 (MQ=255)                                                                                                                                                                   tGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGAGCGTATGGGCGCTGAAATGGTGTTATtg                                                                          <  1:259605/248‑1 (MQ=255)                                                                                                                                                                           gtgGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGgtgt                                                                               >  1:854702/1‑235 (MQ=255)                                                                                                                                                                           gtgGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGGTGTTATtg                                                                          <  1:514846/240‑1 (MQ=255)                                                                                                                                                                           gtgGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGGTGTTATtg                                                                          <  1:354307/240‑1 (MQ=255)                                                                                                                                                                                ggATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGGTGTTATTGCGTGCGTTGGCGttt                                                           >  1:671141/1‑250 (MQ=255)                                                                                                                                                                                 gATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGGTGTTATTGCGTGCGTTGGCGTTTCa                                                         >  1:676298/1‑251 (MQ=255)                                                                                                                                                                                 gATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGGTGTTATTGCGTGCGTTGGCGTTTCa                                                         >  1:529589/1‑251 (MQ=255)                                                                                                                                                                                 gATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCAT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATggg                                                                                             <  1:121439/215‑1 (MQ=255)                                                                                                                                                                                   tAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGGTGTTATTGCGTGCGTTGGCGTTTCAcc                                                       <  1:549666/251‑1 (MQ=255)                                                                                                                                                                                              aTTGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGGTGTTATTGCGTGCGTTGGCGTTTCACCCGAAATTAATg                                            <  1:335395/251‑1 (MQ=255)                                                                                                                                                                                               ttGGGATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGGTGTTATTGCGTGCGTTGGCGTTTCACCCGAAATTAATgg                                           >  1:6335/1‑251 (MQ=255)                                                                                                                                                                                                 gggATGAATTGCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCAT‑CAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGGTGTTATtgcg                                                                        >  1:394354/1‑219 (MQ=255)                                                                                                                                                                                                           gCTACGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATg                                                                                   <  1:88149/199‑1 (MQ=255)                                                                                                                                                                                                              aCGTGAAGTGGCGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGGTGTTATTGCGTGCGTTGGCGTTTCACCCGAAATTAATGGGGGATTTTg                                  >  1:527040/1‑245 (MQ=255)                                                                                                                                                                                                                      tggcGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGGTGTTATTGCGTGCGTTGGCGTTTCACCCGAAATTAATGGGGGATTTTGCGAATGcagaac                      <  1:99173/246‑1 (MQ=255)                                                                                                                                                                                                                      tggcGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGGTGTTATTGCGTGCGTTGGCGTTTCACCCGAAATTAATGGGGGATTTTGCGAATGcagaac                      <  1:103525/246‑1 (MQ=255)                                                                                                                                                                                                                         cGGAAAACTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGGTGTTATTGCGTGCGTTGGCGTTTCACCCGAAATTAATGGGGGATTTTGCGAATGCagaacagaac                 <  1:737993/251‑1 (MQ=255)                                                                                                                                                                                                                              aaCTTGCACAAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAG‑CAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGGTGTTATTGCGTGCGTTGGCGTTTCACCCGAAATTAATGGGGGATTTTGCGAATGCAGAACAGAACGAATTc           <  1:265472/251‑1 (MQ=255)                                                                                                                                                                                                                                    cacaAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGGTGTTATTGCGTGCGTTGGCGTTTCACCCGAAATTAATGGGGGATTTTGCGAATGCAGAACAGAACGAATTCAAtcc      <  1:770155/251‑1 (MQ=255)                                                                                                                                                                                                                                    cacaAAATCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGGTGTTATTGCGTGCGTTGGCGTTTCACCCGAAATTAATGGGGGATTTTGCGAATGCAGAACAGAACGAATTCAAtcc      <  1:513816/251‑1 (MQ=255)                                                                                                                                                                                                                                       aaaaTCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGGTGTTATTGCGTGCGTTGGCGTTTCACCCGAAATTAATGGGGGATTTTGCGAATGCAGAACAGAACGAATTCAAtcctcc   <  1:151348/251‑1 (MQ=255)                                                                                                                                                                                                                                        aaaTCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGGTGTTATTGCGTGCGTTGGCGTTTCACCCGAAATTAATGGGGGATTTTGCGAATGCAGAACAGAACGAATTCAATCCTCCg  >  1:785337/1‑251 (MQ=255)                                                                                                                                                                                                                                        aaaTCGCCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGGTGTTATTGCGTGCGTTGGCGTTTCACCCGAAATTAATGGGGGATTTTGCGAATGCAGAACAGAACGAATTCAATCCTCCg  >  1:587575/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                       TTCGCGACAGTTTAAGCCTCACCGATCAAGCGATAGCCATGAGCAACGGCAATGTTACGCTCAATATCGTTAACGATATGCTCGGTTTACTGGATGAAAGTCAAGCCATTGAGATGATTTATGCGTTACAGCAAGGTAACGGCGAAAAACTGATGCAGGCAGTGAATGCTGTGGCGGATAAAGCCGGCGATTGGGATGAATTGCTACGTGAAGTGTCGGAAAACTTGCACAAAATCGTCATGCAACAACTCTT‑GCCGCAGGCTGCCTTGGATGAAAGCAGTCAAATCGGCTTTTTAG‑CCAAACATATTGCGCCTCAGGACGTTCAATTTTTCTACCAGGTCGTTGTCACCGGTCGTAAAGAATTAAGTGCCGCACCAACGGCGCGTATGGGCGCTGAAATGGTGTTATTGCGTGCGTTGGCGTTTCACCCGAAATTAATGGGGGATTTTGCGAATGCAGAACAGAACGAATTCAATCCTCCG  >  1/601747‑602228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑