Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	597,850	A→G	D42G (GAC→GGC)	unknown →	Penicillin‑insensitive murein endopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	597,850	0	A	G	100.0%	216.7 / NA	77	D42G (GAC→GGC)	unknown	Penicillin‑insensitive murein endopeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/42);  total (35/42)

ATCCTTGTGTCGGCATCCGCGCCGTGCCGATTGCGGAAGCTATGACGGCGATTGTATTGTTGGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGACGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACGGACACCCTGACATGATTAGCTACTTGCAACGTTTGGGGCAACGCGCCAAGGCGGCGGGTTTGCCGACCATGTTGGTGGGCGATATTGCTATGCCGGGCGGCGGACGTTTTCTGAC  >  1/597607‑598081                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                         aTCCTTGTGTCGGCATCCGCGCCGTGCCGATTGCGGAAGCTATGACGGCGATTGTATTGTTGGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGaaa                                                                                                                                                                                                                                   >  1:28825/1‑251 (MQ=255) aTCCTTGTGTCGGCATCCGCGCCGTGCCGATTGCGGAAGCTATGACGGCGATTGTATTGTTGGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGaaa                                                                                                                                                                                                                                   >  1:595577/1‑251 (MQ=255)     ttGTGTCGGCATCCGCGCCGTGCCGATTGCGGAAGCTATGACGGCGATTGTATTGTTGGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCg                                                                                                                                                                                                                                >  1:832701/1‑250 (MQ=255)          tCGGCATCCGCGCCGTGCCGATTGCGGAAGCTATGACGGCGATTGTATTGTTGGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGc                                                                                                                                                                                                                           <  1:582484/250‑1 (MQ=255)                tCCGCGCCGTGCCGATTGCGGAAGCTATGACGGCGATTGTATTGTTGGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGc                                                                                                                                                                                                                    <  1:651263/251‑1 (MQ=255)                         tGCCGATTGCGGAAGCTATGACGGCGATTGTATTGTTGGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTcc                                                                                                                                                                                                           <  1:570219/251‑1 (MQ=255)                               ttGCGGAAGCTATGACGGCGATTGTATTGTTGGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCACCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGt                                                                                                                                                                                                     >  1:436877/1‑251 (MQ=255)                                     aaGCTATGACGGCGATTGTATTGTTGGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTAt                                                                                                                                                                                                <  1:97628/250‑1 (MQ=255)                                     aaGCTATGACGGCGATTGTATTGTTGGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTAt                                                                                                                                                                                                <  1:68599/250‑1 (MQ=255)                                     aaGCTATGACGGCGATTGTATTGTTGGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTAt                                                                                                                                                                                                <  1:839605/250‑1 (MQ=255)                                      aGCTATGACGGCGATTGTATTGTTGGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTAt                                                                                                                                                                                                <  1:172935/249‑1 (MQ=255)                                      aGCTATGACGGCGATTGTATTGTTGGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCa                                                                                                                                                                                              >  1:684759/1‑251 (MQ=255)                                      aGCTATGACGGCGATTGTATTGTTGGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCa                                                                                                                                                                                              >  1:59162/1‑251 (MQ=255)                                      aGCTATGACGGCGATTGTATTGTTGGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCa                                                                                                                                                                                              >  1:393442/1‑251 (MQ=255)                                        cTATGACGGCGATTGTATTGTTGGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCa                                                                                                                                                                                              <  1:846258/249‑1 (MQ=255)                                        cTATGACGGCGATTGTATTGTTGGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCa                                                                                                                                                                                              >  1:506465/1‑249 (MQ=255)                                                 cGATTGTATTGTTGGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACgg                                                                                                                                                                                                      >  1:504324/1‑232 (MQ=255)                                                   attgtattgtTGGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGc                                                                                                                                                                                  >  1:692853/1‑250 (MQ=255)                                                    ttgtattgtTGGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCt                                                                                                                                                                                <  1:783478/251‑1 (MQ=255)                                                      gtattgtTGGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCa                                                                                                                                                                                              >  1:528913/1‑235 (MQ=255)                                                      gtattgtTGGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCttt                                                                                                                                                                              >  1:87776/1‑251 (MQ=255)                                                      gtattgtTGGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCttt                                                                                                                                                                              >  1:786344/1‑251 (MQ=255)                                                      gtattgtTGGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCttt                                                                                                                                                                              >  1:676429/1‑251 (MQ=255)                                                        attgtTGGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTg                                                                                                                                                                             >  1:788879/1‑250 (MQ=255)                                                         ttgttgGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGcc                                                                                                                                                                           <  1:637525/251‑1 (MQ=255)                                                           gttgGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCtt                                                                                                                                                                         >  1:117322/1‑251 (MQ=255)                                                           gttgGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCtt                                                                                                                                                                         >  1:725577/1‑251 (MQ=255)                                                              ggATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTtata                                                                                                                                                                      >  1:64401/1‑251 (MQ=255)                                                                aTCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATaa                                                                                                                                                                     <  1:372085/250‑1 (MQ=255)                                                                  cATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAgg                                                                                                                                                                                          >  1:257509/1‑227 (MQ=255)                                                                  cATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAgg                                                                                                                                                                  <  1:418011/251‑1 (MQ=255)                                                                    tttGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCACAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCg                                                                                                                                                                >  1:447926/1‑251 (MQ=255)                                                                            gTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGc                                                                                                                                                                                                                    >  1:318874/1‑191 (MQ=255)                                                                            gTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGt                                                                                                                                                                                                     >  1:563841/1‑206 (MQ=255)                                                                            gTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGcc                                                                                                                                                                           <  1:622809/232‑1 (MQ=255)                                                                                tAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAGCCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAg                                                                                                                                                    <  1:525618/251‑1 (MQ=255)                                                                                tAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAg                                                                                                                                                    <  1:336692/251‑1 (MQ=255)                                                                                tAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAg                                                                                                                                                    <  1:528433/251‑1 (MQ=255)                                                                                tAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAg                                                                                                                                                    <  1:365863/251‑1 (MQ=255)                                                                                            ccGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑Tg                                                                                                                                        <  1:370752/250‑1 (MQ=255)                                                                                                    ccTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAAc                                                                                                                               <  1:776766/251‑1 (MQ=255)                                                                                                       gAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATaa                                                                                                                                                                     >  1:253067/1‑211 (MQ=255)                                                                                                                aTGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACgg                                                                                                                    <  1:409720/250‑1 (MQ=255)                                                                                                                     atcatGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACg                                                                                                                                                                                                       >  1:480731/1‑163 (MQ=255)                                                                                                                     atcatGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAg                                                                                                                                                    <  1:352417/214‑1 (MQ=255)                                                                                                                     atcatGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACgg                                                                                                                    >  1:695488/1‑245 (MQ=255)                                                                                                                     atcatGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACGGACAcc                                                                                                               >  1:202378/1‑250 (MQ=255)                                                                                                                       catGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACGGACAcc                                                                                                               >  1:13474/1‑248 (MQ=255)                                                                                                                          gAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACGGACACCCTGACa                                                                                                         >  1:741824/1‑251 (MQ=255)                                                                                                                          gAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTAACGCCCCCAACGCTTTTGCCGCCCCGCAAGATTGGCAAAAAATTAAAGGTCCGATTCCCCCAAAAGGCGGGAAATCCCAGCCAATCGCCAGTTTTTCCAACGGTTGTATCAAAGCCGCCAAGCCTTTGCCTTATAAAGGCGCCGGCTATCAAGTGATTC‑CACTGATTAAAAACCCCTATTACGAACACCCTAACa                                                                                                         >  1:239903/1‑251 (MQ=255)                                                                                                                           aaTAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACGGACACCCTGACa                                                                                                         <  1:815745/250‑1 (MQ=255)                                                                                                                           aaTAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACGGACACCCTGACAt                                                                                                        <  1:473634/251‑1 (MQ=255)                                                                                                                           aaTAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACGGACACCCTGACAt                                                                                                        <  1:278444/251‑1 (MQ=255)                                                                                                                              aaaaCCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACGGACACCCTGACATGAt                                                                                                     <  1:475030/251‑1 (MQ=255)                                                                                                                                    tttCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACGGACACCCTGACATGATTAGCTa                                                                                               <  1:401418/251‑1 (MQ=255)                                                                                                                                    tttCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACGGACACCCTGACATGATTAGCTa                                                                                               <  1:41664/251‑1 (MQ=255)                                                                                                                                       ctcCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGAAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAgg                                                                                                                                                                  <  1:318119/182‑1 (MQ=255)                                                                                                                                            aTTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACGGACACCCTGACAt                                                                                                        <  1:143953/234‑1 (MQ=255)                                                                                                                                             ttCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGcc                                                                                                                                                                           <  1:174434/167‑1 (MQ=255)                                                                                                                                              tcatcaAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACGGACACCCTGACATGATTAGCTACTTGCAACGt                                                                                     <  1:779753/251‑1 (MQ=255)                                                                                                                                               catcaAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAg                                                                                                                                                    >  1:655993/1‑188 (MQ=255)                                                                                                                                               catcaAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACGGACACCCTGACATGATTAGCTACTTGCAACGtt                                                                                    >  1:79430/1‑251 (MQ=255)                                                                                                                                                atcaAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACGGACACCCTGACATGATTAGCTACTTGCAACGttt                                                                                   <  1:665436/251‑1 (MQ=255)                                                                                                                                                     aaGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACGGACACCCTGACATGATTAGCTACTTGCAACGTTTGGGGc                                                                              <  1:486449/251‑1 (MQ=255)                                                                                                                                                                         gcGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAg                                                                                                                                                                                           >  1:45301/1‑123 (MQ=255)                                                                                                                                                                                            cGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACGGac                                                                                                                  <  1:97055/176‑1 (MQ=255)                                                                                                                                                                                              cTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGcc                                                                                                                                                                           <  1:793404/118‑1 (MQ=255)                                                                                                                                                                                                       cgccgcAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACGGACACCCTGACATGATTAGCTACTTGCAACGTTTGGGGCAACGCGCCAAGGCGGCGGGTTTGCCGACCATGTTGGTGGGCGATATTGc                             <  1:858620/250‑1 (MQ=255)                                                                                                                                                                                                         ccgcAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCa                                                                                                                                                      <  1:358595/128‑1 (MQ=255)                                                                                                                                                                                                         ccgcAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACGGACACCCTGACa                                                                                                         >  1:897005/1‑172 (MQ=255)                                                                                                                                                                                                         ccgcAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACGGACACCCTGACATGATTAGCTACTTGCAACGTTTGGGGCAACGCGCCAAGGCGGCGGGTTTGCCGACCATGTTGGTGGGCGATATTGCTAt                          >  1:113461/1‑251 (MQ=255)                                                                                                                                                                                                            cAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACGGACACCCTGACATGATTAGCTACTTGCAACGTTTGGGGCAACGCGCCAAGGCGGCGGGTTTGCCGACCATGTTGGTGGGCGATATTGCTATGc                        <  1:260197/250‑1 (MQ=255)                                                                                                                                                                                                            cAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACGGACACCCTGACATGATTAGCTACTTGCAACGTTTGGGGCAACGCGCCAAGGCGGCGGGTTTGCCGACCATGTTGGTGGGCGATATTGCTATGc                        <  1:182247/250‑1 (MQ=255)                                                                                                                                                                                                            cAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACGGACACCCTGACATGATTAGCTACTTGCAACGTTTGGGGCAACGCGCCAAGGCGGCGGGTTTGCCGACCATGTTGGTGGGCGATATTGCTATGc                        <  1:728986/250‑1 (MQ=255)                                                                                                                                                                                                                aTTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACGGACACCCTGACATGATTAGCTACTTGCAACGTTTggg                                                                                <  1:817410/190‑1 (MQ=255)                                                                                                                                                                                                                            ttAAACGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACGGACACCCTGACATGATTAGCTACTTGCAACGTTTGGGGCAACGCGCCAAGGCGGCGGGTTTGCCGACCATGTTGGTGGGCGATATTGCTATGc                        >  1:882240/1‑234 (MQ=255)                                                                                                                                                                                                                                 cGTCCGATTCCCGCAGAAGGCGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCTTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACGGACACCCTGACATGATTAGCTACTTGCAACGTTTGGGGCAACGCGCCAAGGCGGCGGGTTTGCCGACCATGTTGGTGGGCGATATTGCTATGCCGGGCGGCGGACGTTTTCTGAc  <  1:571433/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                         ATCCTTGTGTCGGCATCCGCGCCGTGCCGATTGCGGAAGCTATGACGGCGATTGTATTGTTGGATCATTTGTTGCGTTTTAAAGCGCAATGCCGATAAACCTGAAAAACGGATGCCATCATGAATAAAACCTTTCTCCAATTCATCAAAAGTGCGGTCGTTTTCAGCAGCGTTTTTTACGCCGCCAACGCTTTTGCCGCGCCGCAAGATTGGCAACAAATTAAACGTCCGATTCCCGCAGAAGACGGGAAATCGCAGGCTATCGGCAGTTATTCCAACGGTTGTATCATAGGCGCCCAGCCTTTGCCTTATAAAGGCGACGGCTATCAAGTGATTCGCA‑TGAATAAAAACCGCTATTACGGACACCCTGACATGATTAGCTACTTGCAACGTTTGGGGCAACGCGCCAAGGCGGCGGGTTTGCCGACCATGTTGGTGGGCGATATTGCTATGCCGGGCGGCGGACGTTTTCTGAC  >  1/597607‑598081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑