Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	579,880	T→C	A167A (GCT→GCC)	unknown →	Membrane protein LAPB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	579,880	0	T	C	100.0%	221.2 / NA	77	A167A (GCT→GCC)	unknown	Membrane protein LAPB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/38);  total (39/38)

GCGATTGAAACCAATTTTGCCGTGGCGGAAAAACAATTCGGTAAGGCGTTATGCGAAACGTTAATCGCGCAACAGCCGGTATTGCTATCACAGTTAACCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCTTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGCAAGGCATTCCGCATAAGCGTCAGGCGAAATACCGTTGGAACGATTCGGCGAAATTGTTGTTTAACTATGATGAATTTTGGCAGGAAAACGAGGGCAAATTAGCGGAAA  >  1/579641‑580124                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     gCGATTGAAACCAATTTTGCCGTGGCGGAAAAACAATTCGGTAAGGCGTTATGCGAAACGTTAATCGCGCAACAGCCGGTATTGCTATCACAGTTAACCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTc                                                                                                                                                                                                                                               <  1:691199/247‑1 (MQ=255)        aaaCCAATTTTGCCGTGGCGGAAAAACAATTCGGTAAGGCGTTATGCGAAACGTTAATCGCGCAACAGCCGGTATTGCTATCACAGTTAACCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCaaaa                                                                                                                                                                                                                                    >  1:357008/1‑251 (MQ=255)              aTTTTGCCGTGGCGGAAAAACAATTCGGTAAGGCGTTATGCGAAACGTTAATCGCGCAACAGCCGGTATTGCTATCACAGTTAACCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGAt                                                                                                                                                                                                                              >  1:237991/1‑251 (MQ=255)                       tGGCGGAAAAACAATTCGGTAAGGCGTTATGCGAAACGTTAATCGCGCAACAGCCGGTATTGCTATCACAGTTAACCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAg                                                                                                                                                                                                                     <  1:90019/251‑1 (MQ=255)                                       cGGTAAGGCGTTATGCGAAACGTTAATCGCGCAACAGCCGGTATTGCTATCACAGTTAACCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTCCCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAgg                                                                                                                                                                                                     >  1:440511/1‑251 (MQ=255)                                        ggTAAGGCGTTATGCGAAACGTTAATCGCGCAACAGCCGGTATTGCTATCACAGTTAACCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAgg                                                                                                                                                                                                     >  1:545755/1‑250 (MQ=255)                                         gTAAGGCGTTATGCGAAACGTTAATCGCGCAACAGCCGGTATTGCTATCACAGTTAACCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGAc                                                                                                                                                                                                                                         <  1:588598/213‑1 (MQ=255)                                              gCGTTATGCGAAACGTTAATCGCGCAACAGCCGGTATTGCTATCACAGTTAACCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGa                                                                                                                                                                                                                                          >  1:748980/1‑207 (MQ=255)                                              gCGTTATGCGAAACGTTAATCGCGCAACAGCCGGTATTGCTATCACAGTTAACCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACaaa                                                                                                                                                                                              >  1:317407/1‑251 (MQ=255)                                                 ttATGCGAAACGTTAATCGCGCAACAGCCGGTATTGCTATCACAGTTAACCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGc                                                                                                                                                                                           <  1:505947/251‑1 (MQ=255)                                                   aTGCGAAACGTTAATCGCGCAACAGCCGGTATTGCTATCACAGTTAACCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCAt                                                                                                                                                                                         <  1:827561/251‑1 (MQ=255)                                                             ttAATCGCGCAACAGCCGGTATTGCTATCACAGTTAACCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCg                                                                                                                                                                                <  1:180317/250‑1 (MQ=255)                                                                  cgcgCAACAGCCGGTATTGCTATCACAGTTAACCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGcc                                                                                                                                                                          <  1:211877/251‑1 (MQ=255)                                                                  cgcgCAACAGCCGGTATTGCTATCACAGTTAACCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGcc                                                                                                                                                                          <  1:543863/251‑1 (MQ=255)                                                                             cGGTATTGCTATCACAGTTAACCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATg                                                                                                                                                               <  1:149476/251‑1 (MQ=255)                                                                             cGGTATTGCTATCACAGTTAACCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATg                                                                                                                                                               <  1:522671/251‑1 (MQ=255)                                                                               gTATTGCTATCACAGTTAACCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCACGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGtt                                                                                                                                                             >  1:638206/1‑251 (MQ=255)                                                                                   tGCTATCACAGTTAACCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGc                                                                                                                                                                                      >  1:885396/1‑222 (MQ=255)                                                                                      tATCACAGTTAACCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGcc                                                                                                                                                                          <  1:550844/231‑1 (MQ=255)                                                                                       aTCACAGTTAACCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATg                                                                                                                                                     <  1:483892/251‑1 (MQ=255)                                                                                             gTTAACCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTa                                                                                                                                               >  1:702491/1‑251 (MQ=255)                                                                                             gTTAACCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTa                                                                                                                                               >  1:359104/1‑251 (MQ=255)                                                                                                 aCCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCAt                                                                                                                                                                                    <  1:518204/210‑1 (MQ=255)                                                                                                 aCCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAgg                                                                                                                                            <  1:541151/250‑1 (MQ=255)                                                                                                 aCCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGt                                                                                                                                           <  1:90114/251‑1 (MQ=255)                                                                                                 aCCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGt                                                                                                                                           <  1:414323/251‑1 (MQ=255)                                                                                                 aCCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGt                                                                                                                                           >  1:507626/1‑251 (MQ=255)                                                                                                  ccGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTa                                                                                                                                          <  1:289266/251‑1 (MQ=255)                                                                                                       gAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTgg                                                                                                                                      >  1:269430/1‑250 (MQ=255)                                                                                                       gAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTgg                                                                                                                                      >  1:785457/1‑250 (MQ=255)                                                                                                             gCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCACGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAgg                                                                                                                               >  1:748889/1‑250 (MQ=255)                                                                                                                    aTTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTAc                                                                                                                          <  1:357956/249‑1 (MQ=255)                                                                                                                    aTTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACAt                                                                                                                        <  1:38939/251‑1 (MQ=255)                                                                                                                    aTTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACAt                                                                                                                        >  1:504064/1‑251 (MQ=255)                                                                                                                    aTTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACAt                                                                                                                        >  1:68148/1‑251 (MQ=255)                                                                                                                    aTTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACAt                                                                                                                        <  1:104490/251‑1 (MQ=255)                                                                                                                       tAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGt                                                                                                                     <  1:60477/251‑1 (MQ=255)                                                                                                                              gAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTg                                                                                                               >  1:526222/1‑250 (MQ=255)                                                                                                                              gAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTg                                                                                                               >  1:730216/1‑250 (MQ=255)                                                                                                                               aaTCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGc                                                                                                              <  1:267133/250‑1 (MQ=255)                                                                                                                                aTCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGCaa                                                                                                            >  1:260481/1‑251 (MQ=255)                                                                                                                                aTCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGCaa                                                                                                            >  1:543966/1‑251 (MQ=255)                                                                                                                                          cccTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGCAAGGCATTCCGc                                                                                                  <  1:597809/251‑1 (MQ=255)                                                                                                                                           ccTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAgg                                                                                                                                            >  1:523678/1‑208 (MQ=255)                                                                                                                                            cTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGCAAGGCATTCCGCAt                                                                                                <  1:721526/251‑1 (MQ=255)                                                                                                                                             ttACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGCAAGGCATTCCGCATa                                                                                               >  1:714733/1‑251 (MQ=255)                                                                                                                                               aCGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGCAAGGCATTCCGCATAAg                                                                                             <  1:174862/251‑1 (MQ=255)                                                                                                                                                  aaGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGCAAGGCATTCCGCATAAGCg                                                                                           <  1:519940/250‑1 (MQ=255)                                                                                                                                                                  gggTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGt                                                                                                                                           <  1:494603/186‑1 (MQ=255)                                                                                                                                                                  gggTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGCAAGGCATTCCGCATAAGCGTCAGGCGAAATACCGtt                                                                          >  1:637701/1‑251 (MQ=255)                                                                                                                                                                  gggTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGCAAGGCATTCCGCATAAGCGTCAGGCGAAATACCGtt                                                                          >  1:161575/1‑251 (MQ=255)                                                                                                                                                                  gggTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGCAAGGCATTCCGCATAAGCGTCAGGCGAAATACCGtt                                                                          >  1:179206/1‑251 (MQ=255)                                                                                                                                                                   ggTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTc                                                                                                                                                                                                                                               <  1:570301/85‑1 (MQ=255)                                                                                                                                                                   ggTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAgg                                                                                                                                            <  1:508456/184‑1 (MQ=255)                                                                                                                                                                   ggTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGt                                                                                                                                           <  1:221819/185‑1 (MQ=255)                                                                                                                                                                   ggTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGCAAGGCATTCCGCATAAGCGTCAGGCGAAATACCGTTg                                                                         >  1:12679/1‑251 (MQ=255)                                                                                                                                                                       tAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTc                                                                                                                                                                                                                                               <  1:289553/81‑1 (MQ=255)                                                                                                                                                                        aaCAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGCAAGGCATTCCGCATAAGCGTCAGGCGAAATACCGTTGGAACg                                                                    <  1:612526/251‑1 (MQ=255)                                                                                                                                                                                         gcagcaTTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATg                                                                                                                                                               >  1:521245/1‑143 (MQ=255)                                                                                                                                                                                          cagcaTTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGt                                                                                                                                           <  1:233421/162‑1 (MQ=255)                                                                                                                                                                                            gcaTTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGCAAGGCATTCCGCATAAg                                                                                             <  1:53051/206‑1 (MQ=255)                                                                                                                                                                                            gcaTTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGCAAGGCATTCCGCATAAGCGTCAGGCGAAATACCGTTGGAACGATTCGGCGAAATTGTTGttt                                                >  1:78997/1‑251 (MQ=255)                                                                                                                                                                                            gcaTTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGCAAGGCATTCCGCATAAGCGTCAGGCGAAATACCGTTGGAACGATTCGGCGAAATTGTTGttt                                                >  1:75199/1‑251 (MQ=255)                                                                                                                                                                                             caTTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAg                                                                                                                                                                                            >  1:267464/1‑110 (MQ=255)                                                                                                                                                                                                 tATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGCAAGGCATTCCGCATAAGCGTCAGGCGAAATACCGTTGGAACGATTCGGCGAAATTGTTGTTTAACTa                                           <  1:103112/251‑1 (MQ=255)                                                                                                                                                                                                                aCCTTTTTAGCGAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGCAAGGCATTCCGCATAAGCGTCAGGCGAAATACCGTTGGAACGATTCGGCGAAATTGTTGTTTAACTATGATGAATTTTGGCa                            <  1:615679/251‑1 (MQ=255)                                                                                                                                                                                                                           gAATAATCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGc                                                                                                                                                    <  1:271111/120‑1 (MQ=255)                                                                                                                                                                                                                                atCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTa                                                                                                                                               >  1:97801/1‑120 (MQ=255)                                                                                                                                                                                                                                atCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGc                                                                                                              <  1:455506/153‑1 (MQ=255)                                                                                                                                                                                                                                atCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGCAAGGCATTCCGCATAAGCg                                                                                           >  1:555832/1‑172 (MQ=255)                                                                                                                                                                                                                                atCGCGTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGCAAGGCATTCCGCATAAGCGTCAGGCGAAATACCGTTGGAACGATTCGGCGAAATTGTTGTTTAACTATGATGAATTTTGGCAGGAAAACGAGGGCaaa            >  1:342214/1‑251 (MQ=255)                                                                                                                                                                                                                                  cgcgTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGCAAGGCATTCCGCATAAGCGTCAGGCGAAATACCGTTGGAACGATTCGGCGAAATTGTTGTTTAACTATGATGAATTTTGGCAGGAAAACGAGGGCAAAtt          >  1:564507/1‑251 (MQ=255)                                                                                                                                                                                                                                     gTATTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGCAAGGCATTCCGCATAAGCGTCAGGCGAAATACCGTTGGAACGATTCGGCGAAATTGTTGTTTAACTATGATGAAttt                                 >  1:757460/1‑225 (MQ=255)                                                                                                                                                                                                                                       aTTGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGCAAGGCATTCCGCATAAGCGTCAGGCGAAATACCGTTGGAACGATTCGGCGAAATTGTTGTTTAACTATGATGAATTTTGGCAGGAAAACGAGGGCAAATTAGCgg     >  1:208962/1‑251 (MQ=255)                                                                                                                                                                                                                                        ttGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGCAAGGCATTCCGCATAAGCGTCAGGCGAAATACCGTTGGAACGATTCGGCGAAATTGTTGTTTAACTATGATGAATTTTGGCAGGAAAACGAGGGCAAATTAGCGGa    >  1:200706/1‑251 (MQ=255)                                                                                                                                                                                                                                        ttGATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGCAAGGCATTCCGCATAAGCGTCAGGCGAAATACCGTTGGAACGATTCGGCGAAATTGTTGTTTAACTATGATGAATTTTGGCAGGAAAACGAGGGCAAATTAGCGGa    >  1:74830/1‑251 (MQ=255)                                                                                                                                                                                                                                          gATCGCCTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGCAAGGCATTCCGCATAAGCGTCAGGCGAAATACCGTTGGAACGATTCGGCGAAATTGTTGTTTAACTATGATGAATTTTGGCAGGAAAACGAGGGCAAATTAGCGGaaa  >  1:200102/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     GCGATTGAAACCAATTTTGCCGTGGCGGAAAAACAATTCGGTAAGGCGTTATGCGAAACGTTAATCGCGCAACAGCCGGTATTGCTATCACAGTTAACCGATGAGTTAGCGTTAAATTTAACGCTGAATCATATCGACCCTTACGAAGGTTTTTTTGCCATGGGTTTAACAGATGCAAATCAACGCAGCATTTATTCTGCCGGTTTTACCTTTTTAGCGAATAATCGCGTATTGATCGCTTCCATTCAGGGACCAAAAGGCGATGAAGCGCAGGATTTGGTGCGTCAGGCAACAAAAGCATTGCATGGCGTGCGCCCGATGTTTATGTTGGTGAATGCGTTTAAGGTATTGGCGGAAGTATTACATTGTCGCTTGCAAGGCATTCCGCATAAGCGTCAGGCGAAATACCGTTGGAACGATTCGGCGAAATTGTTGTTTAACTATGATGAATTTTGGCAGGAAAACGAGGGCAAATTAGCGGAAA  >  1/579641‑580124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑