Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	577,066	A→G	A674A (GCT→GCC)	unknown ←	Outer membrane receptor proteins, mostly Fe transport

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	577,066	0	A	G	100.0%	184.3 / NA	67	A674A (GCT→GCC)	unknown	Outer membrane receptor proteins, mostly Fe transport
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/36);  total (31/36)

ACATTTAGTATGTCAACTTGCAAATAAATACTGTGATTACCGGTAATGGTCGGTTGCCAACGAATACTACTGTCCCATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGGAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGAGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATCCGCCCGTTTGGTTTTTAACCAATCAAATCCCAGAGAGCTTGTCCAGTAGCTTTTACCCAATTGCCAAGGTTCAATATTATTCATTTGGAAAGTGTAAACGTCGACGCTGAAGTC  >  1/576833‑577290                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                 aCATTTAGTATGTCAACTTGCAAATAAATACTGTGATTACCGGTAATGGTCGGTTGCCAACGAATACTACTGTCCCATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGt                                                                                                                                                                                                                 <  1:686080/251‑1 (MQ=255)  cATTTAGTATGTCAACTTGCAAATAAATACTGTGATTACCGGTAATGGTCGGTTGCCAACGAATACTACTGTCCCATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGtct                                                                                                                                                                                                                      >  1:881904/1‑245 (MQ=255)      tAGTATGTCAACTTGCAAATAAATACTGTGATTACCGGTAATGGTCGGTTGCCAACGAATACTACTGTCCCATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGaa                                                                                                                                                                                                             >  1:341469/1‑250 (MQ=255)         tATGTCAACTTGCAAATAAATACTGTGATTACCGGTAATGGTCGGTTGCCAACGAATACTACTGTCCCATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTa                                                                                                                                                                                                          <  1:759511/250‑1 (MQ=255)                     cAAATAAATGCTGTGATTACCGGTAATGGTCGGTTGCCAACGAATACTACTGTCCCATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATGCAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATGGCCATATCCAAGCCGAAGCGGTGAATCCAGTCTTCTGTAGAACTATTGACTTTGTTc                                                                                                                                                                                              <  1:811445/250‑1 (MQ=255)                       aataaataCTGTGATTACCGGTAATGGTCGGTTGCCAACGAATACTACTGTCCCATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGt                                                                                                                                                                                                                        >  1:353234/1‑222 (MQ=255)                       aataaataCTGTGATTACCGGTAATGGTCGGTTGCCAACGAATACTACTGTCCCATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAg                                                                                                                                                                                                               >  1:399411/1‑231 (MQ=255)                         taaataCTGTGATTACCGGTAATGGTCGGTTGCCAACGAATACTACTGTCCCATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGt                                                                                                                                                                                                                 <  1:768702/227‑1 (MQ=255)                            ataCTGTGATTACCGGTAATGGTCGGTTGCCAACGAATACTACTGTCCCATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCAttt                                                                                                                                                                                       >  1:21562/1‑250 (MQ=255)                              aCTGTGATTACCGGTAATGGTCGGTTGCCAACGAATACTACTGTCCCATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTtctc                                                                                                                                                                                    <  1:184651/251‑1 (MQ=255)                                tgtgATTACCGGTAATGGTCGGTTGCCAACGAATACTACTGTCCCATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTg                                                                                                                                                                                  <  1:470711/251‑1 (MQ=255)                                   gATTACCGGTAATGGTCGGTTGCCAACGAATACTACTGTCCCATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCgg                                                                                                                                                                               >  1:344682/1‑251 (MQ=255)                                           gTAATGGTCGGTTGCCAACGAATACTACTGTCCCATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGgtaagtaa                                                                                                                                                                       >  1:398182/1‑251 (MQ=255)                                              aTGGTCGGTTGCCAACGAATACTACTGTCCCATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAAttt                                                                                                                                                                    >  1:811268/1‑251 (MQ=255)                                                  tCGGTTGCCAACGAATACTACTGTCCCATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTAcc                                                                                                                                                                 <  1:243049/250‑1 (MQ=255)                                                     gTTGCCAACGAATACTACTGTCCCATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGgtaagtaa                                                                                                                                                                       <  1:602465/241‑1 (MQ=255)                                                             cGAATACTACTGTCCCATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTtctc                                                                                                                                                                                    <  1:618460/220‑1 (MQ=255)                                                              gAATACTACTGTCCCATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGt                                                                                                                                                                                                                 >  1:894319/1‑190 (MQ=255)                                                               aaTACTACTGTCCCATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACt                                                                                                                                                    <  1:305265/250‑1 (MQ=255)                                                               aaTACTACTGTCCCATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACt                                                                                                                                                    <  1:793800/250‑1 (MQ=255)                                                                   ctactGTCCCATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTAcc                                                                                                                                                                 <  1:649219/233‑1 (MQ=255)                                                                   ctactGTCCCATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAAtt                                                                                                                                                >  1:300878/1‑250 (MQ=255)                                                                   ctactGTCCCATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAAtt                                                                                                                                                >  1:696527/1‑250 (MQ=255)                                                                        gTCCCATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCAt                                                                                                                                                               <  1:307716/230‑1 (MQ=255)                                                                          cccATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACt                                                                                                                                                                                                    >  1:787950/1‑191 (MQ=255)                                                                          cccATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTtctc                                                                                                                                                                                    >  1:330737/1‑207 (MQ=255)                                                                                gggTGTGTCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGa                                                                                                                                  >  1:694194/1‑251 (MQ=255)                                                                                  gtgtgtCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGAt                                                                                                                                 <  1:586947/250‑1 (MQ=255)                                                                                  gtgtgtCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGAt                                                                                                                                 <  1:538092/250‑1 (MQ=255)                                                                                    gtgtCGGCCGTAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAAc                                                                                                                                                     <  1:846522/228‑1 (MQ=255)                                                                                              tAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATc                                                                                                                    <  1:863766/251‑1 (MQ=255)                                                                                              tAGTGATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATc                                                                                                                    <  1:883614/251‑1 (MQ=255)                                                                                                  gATAAGAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGgtaagtaa                                                                                                                                                                       >  1:182834/1‑196 (MQ=255)                                                                                                       gAACGATAACGGAAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATc                                                                                                                    <  1:142347/242‑1 (MQ=255)                                                                                                                cggaaaaTTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTtctc                                                                                                                                                                                    <  1:344308/165‑1 (MQ=255)                                                                                                                cggaaaaTTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATCCGCCCGTTTGGTTTTTaa                                                                                                  >  1:816735/5‑251 (MQ=255)                                                                                                                                 aaaaTCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATCCGCCCGTTTGGTTTTTAACCAATCAAATCCCagag                                                                                 <  1:269344/251‑1 (MQ=255)                                                                                                                                    aTCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATCGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATCCGCCCGTTTGGTTTTTAACCAATCAAATCCCAGAGAGc                                                                              <  1:447145/251‑1 (MQ=255)                                                                                                                                                       tAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATc                                                                                                                    >  1:464496/1‑194 (MQ=255)                                                                                                                                                            gCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATCCGCCCGTTTGGTTTTTAACCAATCAAATCCCAGAGAGc                                                                              <  1:283947/227‑1 (MQ=255)                                                                                                                                                                    gTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATc                                                                                                                    <  1:789564/181‑1 (MQ=255)                                                                                                                                                                    gTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATCCGCCCGTTTGGTTTTTAACCAATCAAATCCCAGAGAGCTTGTCCAGTAGCTTTTACCCAATTGCCAAGGt                                              >  1:505954/1‑251 (MQ=255)                                                                                                                                                                      gCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTc                                                                                                                                               >  1:593807/1‑152 (MQ=255)                                                                                                                                                                         tttATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTc                                                                                                                                               <  1:362209/149‑1 (MQ=255)                                                                                                                                                                         tttATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATc                                                                                                                    <  1:86589/176‑1 (MQ=255)                                                                                                                                                                         tttATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATCCGCCCGTTTGGTTTTTAACCAATCAAATCCCAGAGAGCTTGTCCAGTAGCTTTTACCCAATTGCCAAGGTTCAat                                         >  1:148106/1‑251 (MQ=255)                                                                                                                                                                           tatataCAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATCCGCCCGTTTGGTTTTTAACCAATCAAATCCCAGAGAGCTTGTCCAGTAGCTTTTACCCAATTGCCAAGGTTCAatat                                       <  1:546896/251‑1 (MQ=255)                                                                                                                                                                           tatataCAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATCCGCCCGTTTGGTTTTTAACCAATCAAATCCCAGAGAGCTTGTCCAGTAGCTTTTACCCAATTGCCAAGGTTCAatat                                       <  1:407036/251‑1 (MQ=255)                                                                                                                                                                            atataCAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATCCGCCCGTTTGGTTTTTAACCAATCAAATCCCAGAGAGCTTGTCCAGTAGCTTTTACCCAATTGCCAAGGTTCAatat                                       <  1:401913/250‑1 (MQ=255)                                                                                                                                                                             tataCAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATCCGCCCGTTTGGTTTTTAACCAATCAAATCCCAGAGAGCTTGTCCAGTAGCTTTTACCCAATTGCCAAGGTTCAAtatta                                     <  1:287137/251‑1 (MQ=255)                                                                                                                                                                                 cAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATCCGCCCGTTTGGTTTTTAACCAATCAAATCCCAGAGAGCTTGTCCAGTAGCTTTTACCCAATTGCCAAGGTTCAATATTATTCa                                 >  1:604266/1‑251 (MQ=255)                                                                                                                                                                                     ttgtttgACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATCCGCCCGTTTGGTTTTTAACCAATCAAATCCCAGAGAGCTTGTCCAGTAg                                                                   >  1:547789/1‑213 (MQ=255)                                                                                                                                                                                      tgtttgACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATCCGCCCGTTTGGTTTTTAACCAATCAAATCCCAGAGAGCTTGTCCAGTAGCTTTTACCCAATTGCCAAGGTTCAatat                                       >  1:729332/1‑240 (MQ=255)                                                                                                                                                                                          tgACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATCCGCCCGTTTGGTTTTTAACCAATCAAATCCCAGAGAGCTTGTCCAGTAGCTTTTACCCAATTGCCAAGGTTCAATATTATTCATTTGGAAAg                        <  1:41835/251‑1 (MQ=255)                                                                                                                                                                                           gACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATCCGCCCGTTTGGTTTTTAACCAATCAAATCCCAGAGAGCTTGTCCAGTAGCtt                                                                >  1:380211/1‑210 (MQ=255)                                                                                                                                                                                               aaGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATCCGCCCGTTTGGTTTTTAACCAATCAAATCCCAGAGAGCTTGTCCAGTAGCTTTTACCCAATTGCCAAGGTTCAATATTATTCATTTGGAAAGTGTaa                   >  1:863134/1‑251 (MQ=255)                                                                                                                                                                                                 gTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATCCGCCCGTTTGGTTTTTAACCAATCAAATCCCAGAGAGCTTGTCCAGTAGCTTTTACCCAATTGCCAAGGTTCAatat                                       <  1:411809/229‑1 (MQ=255)                                                                                                                                                                                                 gTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATCCGCCCGTTTGGTTTTTAACCAATCAAATCCCAGAGAGCTTGTCCAGTAGCTTTTACCCAATTGCCAAGGTTCAATATTATTCATTTGGAAAGTGTaaa                  >  1:678609/1‑250 (MQ=255)                                                                                                                                                                                                 gTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATCCGCCCGTTTGGTTTTTAACCAATCAAATCCCAGAGAGCTTGTCCAGTAGCTTTTACCCAATTGCCAAGGTTCAATATTATTCATTTGGAAAGTGTaaa                  >  1:176331/1‑250 (MQ=255)                                                                                                                                                                                                    aTGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAg                                                                                                                                                                                                               >  1:744987/1‑58 (MQ=255)                                                                                                                                                                                                                 ggATTGCCATATCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATCCGCCCGTTTGGTTTTTAACCAATCAAATCCCAGAGAGCTTGTCCAGTAGCTTTTACCCAATTGCCAAGGTTCAATATTATTCATTTGGAAAGTGTAAACGTCGACGCTGAAGTc  <  1:731849/250‑1 (MQ=255)                                                                                                                                                                                                                         atatCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTAcc                                                                                                                                                                 >  1:884831/1‑83 (MQ=255)                                                                                                                                                                                                                           atCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACt                                                                                                                                                    <  1:883404/94‑1 (MQ=255)                                                                                                                                                                                                                           atCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATCCGTCCGTTTGGTTTTTAACCAATCAAATCCCAGAGAGCTTGTCCAGTAGCTTTTACCCAATTGCCAAGGTTCAATATTATTCATTTGGAAAGTGTAAACg                >  1:894888/1‑226 (MQ=255)                                                                                                                                                                                                                           atCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATCCGCCCGTTTGGTTTTTAACCAATCAAATCCCAGAGAGCTTGTCCAGTAGCTTTTACCCAATTGCCAAGGTTCAatat                                       <  1:811328/203‑1 (MQ=255)                                                                                                                                                                                                                           atCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATCCGCCCGTTTGGTTTTTAACCAATCAAATCCCAGAGAGCTTGTCCAGTAGCTTTTACCCAATTGCCAAGGTTCAATATTATTc                                  >  1:655689/1‑208 (MQ=255)                                                                                                                                                                                                                           atCCAAGCCGAAGCGGGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATCCGCCCGTTTGGTTTTTAACCAATCAAATCCCAGAGAGCTTGTCCAGTAGCTTTTACCCAATTGCCAAGGTTCAATATTATTCATTTGGAAAGTGTAAACg                <  1:896781/226‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                 ACATTTAGTATGTCAACTTGCAAATAAATACTGTGATTACCGGTAATGGTCGGTTGCCAACGAATACTACTGTCCCATTGGGTGTGTCGGCCGTAGTGATAAGAACGATAACGGGAAATTCCGTCGTTAAAATCACCTTCCAAAACGTCATAACTGCGAATCGGTGCTTTTATATACAATTTGTTTGACCAAGTGATGTTGTAATCGGGGATTGCCATATCCAAGCCGAAGCGAGCAATCCAGTCTTCTGTAGAACTATTGACTTTGTTCAGCATTTCTCTGCGGGTAAGTAATTTACCATCGAGATAAACTAATTCATTGGGATCAAGATCTCGACCTATATCCGCCCGTTTGGTTTTTAACCAATCAAATCCCAGAGAGCTTGTCCAGTAGCTTTTACCCAATTGCCAAGGTTCAATATTATTCATTTGGAAAGTGTAAACGTCGACGCTGAAGTC  >  1/576833‑577290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑