Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 570,985 C→T D174N (GAT→AAT)  unknown ← 5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine methyltransferase (EC 2.1.1.14)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1570,9850CT100.0% 214.2 / NA 79D174N (GAT→AAT) unknown5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine methyltransferase (EC 2.1.1.14)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/48);  total (31/48)

GCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATCAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGTATCCGCGTGGAATTCCGGCACGAGGTAATGATAGTTGGTGTCAAACCATTTGGTCATTTCAATGGCAAATTGTGCTTTGTTACCGCGCGCCAGTTGGAAATATTGATC  >  1/570747‑571228
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   
gCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCAc                                                                                                                                                                                                                                          <  1:129029/250‑1 (MQ=255)
        taaCGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCtt                                                                                                                                                                                                                                 <  1:762656/251‑1 (MQ=255)
           cGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTg                                                                                                                                                                                                                               >  1:723611/1‑250 (MQ=255)
                    ttCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACcaca                                                                                                                                                                                                                     >  1:852249/1‑251 (MQ=255)
                      ccGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATTGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAg                                                                                                                                                                                                                   <  1:516517/251‑1 (MQ=255)
                         cgACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACca                                                                                                                                                                                                                       >  1:549032/1‑244 (MQ=255)
                              gAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTc                                                                                                                                                                                                                                   <  1:237333/227‑1 (MQ=255)
                               aaCCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGt                                                                                                                                                                                                              <  1:557900/247‑1 (MQ=255)
                                    aaaTAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGAc                                                                                                                                                                                                      <  1:881167/250‑1 (MQ=255)
                                           tGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAGACCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGGTTATTTAATAAATCAAAGCGATTAAATGCCGCGCCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACcaacaa                                                                                                                                                                                                <  1:531316/249‑1 (MQ=255)
                                               tAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTgg                                                                                                                                                                                          <  1:260338/251‑1 (MQ=255)
                                                     tAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGt                                                                                                                                                                                        <  1:71176/247‑1 (MQ=255)
                                                        ttttGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACAt                                                                                                                                                                                 <  1:772916/251‑1 (MQ=255)
                                                        ttttGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACAt                                                                                                                                                                                 <  1:196333/251‑1 (MQ=255)
                                                        ttttGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACAt                                                                                                                                                                                 <  1:621918/251‑1 (MQ=255)
                                                            gCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAg                                                                                                                                                                                             <  1:256335/235‑1 (MQ=255)
                                                                 tACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGt                                                                                                                                                                                        <  1:886382/235‑1 (MQ=255)
                                                                   cTTGTGTGATTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAgg                                                                                                                                                                      >  1:644327/1‑251 (MQ=255)
                                                                          gCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAAcc                                                                                                                                                                                                                        >  1:415175/1‑194 (MQ=255)
                                                                          gCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGaa                                                                                                                                                                                                                 <  1:870578/201‑1 (MQ=255)
                                                                          gCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTgg                                                                                                                                                               >  1:247277/1‑251 (MQ=255)
                                                                          gCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTgg                                                                                                                                                               >  1:519061/1‑251 (MQ=255)
                                                                           cTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCGCCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGc                                                                                                                                                              <  1:560285/251‑1 (MQ=255)
                                                                           cTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTgg                                                                                                                                                               >  1:892985/1‑250 (MQ=255)
                                                                                   ggCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCtt                                                                                                                                                                                                                                 <  1:138926/176‑1 (MQ=255)
                                                                                   ggCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAg                                                                                                                                                                                             <  1:434701/212‑1 (MQ=255)
                                                                                   ggCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGCGGGTTTAACATTCAAGCCAAGGGc                                                                                                                                                                    <  1:83573/237‑1 (MQ=255)
                                                                                    gCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTaaa                                                                                                                                                                                                                                                  >  1:127072/1‑158 (MQ=255)
                                                                                    gCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAAt                                                                                                                                                     >  1:782609/1‑251 (MQ=255)
                                                                                           cGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACcaacaa                                                                                                                                                                                                >  1:113887/1‑201 (MQ=255)
                                                                                           cGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATttgttg                                                                                                                                               >  1:209327/1‑250 (MQ=255)
                                                                                           cGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATttgttg                                                                                                                                               >  1:69881/1‑250 (MQ=255)
                                                                                             tCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGc                                                                                                                                                              <  1:73587/233‑1 (MQ=255)
                                                                                               tttATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTc                                                                                                                                                                                                                                   >  1:486104/1‑162 (MQ=255)
                                                                                                ttATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGaa                                                                                                                                                                                                                 <  1:861079/179‑1 (MQ=255)
                                                                                                  ataAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCGCCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAAt                                                                                                                                       <  1:371009/251‑1 (MQ=255)
                                                                                                   taAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATg                                                                                                                                      <  1:427464/251‑1 (MQ=255)
                                                                                                       ccGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAAt                                                                                                                                                                                                          <  1:656058/179‑1 (MQ=255)
                                                                                                       ccGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGcc                                                                                                                                  <  1:812639/251‑1 (MQ=255)
                                                                                                               cATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAg                                                                                                                           >  1:575479/1‑250 (MQ=255)
                                                                                                                aTTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCt                                                                                                                         <  1:20490/251‑1 (MQ=255)
                                                                                                                aTTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCt                                                                                                                         <  1:81138/251‑1 (MQ=255)
                                                                                                                      ccGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGaa                                                                                                                    <  1:169944/250‑1 (MQ=255)
                                                                                                                           aaaTCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGt                                                                                                              <  1:79766/251‑1 (MQ=255)
                                                                                                                           aaaTCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGt                                                                                                              <  1:345573/251‑1 (MQ=255)
                                                                                                                              tCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGTATc                                                                                                           <  1:703876/251‑1 (MQ=255)
                                                                                                                                    gTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCt                                                                                                                         >  1:394019/1‑231 (MQ=255)
                                                                                                                                     ttAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGTATCcgcg                                                                                                       <  1:601303/248‑1 (MQ=255)
                                                                                                                                        aaGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGcc                                                                                                                                                                          <  1:776704/178‑1 (MQ=255)
                                                                                                                                              gTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGTATCCGCGTGGAATTCCgg                                                                                            <  1:327562/250‑1 (MQ=255)
                                                                                                                                                       tttGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGc                                                                                                                                                                                                                                               >  1:457683/1‑94 (MQ=255)
                                                                                                                                                          gAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGt                                                                                                              >  1:269404/1‑220 (MQ=255)
                                                                                                                                                          gAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGTATCCGCGTGGAATTCCGGc                                                                                           <  1:862898/239‑1 (MQ=255)
                                                                                                                                                          gAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGTATCCGCGTGGAATTCCGGCACGAGGTAATGa                                                                               >  1:167910/1‑251 (MQ=255)
                                                                                                                                                           aaTATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGTATCCGCGTGGAATTCCGGCACGAGGTAATGa                                                                               <  1:692801/250‑1 (MQ=255)
                                                                                                                                                           aaTATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGTATCCGCGTGGAATTCCGGCACGAGGTAATGAt                                                                              <  1:398072/251‑1 (MQ=255)
                                                                                                                                                             tataATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGTATCCGCGTGGAATTCCGGCACGAGGTAATGATAg                                                                            <  1:587453/251‑1 (MQ=255)
                                                                                                                                                                 aTCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAAt                                                                                                                                                                                               >  1:707453/1‑132 (MQ=255)
                                                                                                                                                                 aTCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGTATCCGCGTGGAATTCCGGCACGAGGTAATGATAGTTgg                                                                        >  1:681631/1‑251 (MQ=255)
                                                                                                                                                                     aCGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGTATCCGCGTGGAATTCCGGCACGAGGTAATGATAGTTGGTGTc                                                                    <  1:522571/251‑1 (MQ=255)
                                                                                                                                                                         cTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGTATCCGCGTGGAATTCCGGCACGAGGTAATGATAGTTGGTGTCAAAc                                                                <  1:278470/251‑1 (MQ=255)
                                                                                                                                                                                  ccAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGTATCCGCGTGGAATTCCGGCACGAGGTAATGATAGTTGGTGTCAAACCATTTGGTc                                                       <  1:790637/251‑1 (MQ=255)
                                                                                                                                                                                       gCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGTATCCGCGTGGAATTCCGGCACGAGGTAATGATAGTTGGTGTCAAACCATTTGGTCAttt                                                   >  1:472863/1‑250 (MQ=255)
                                                                                                                                                                                         gTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGTATCCGCGTGGAATTCCGGCACGAGGTAATGATAGTTGGTGTCAAACCATTTGGTCATTTCaa                                                >  1:15001/1‑251 (MQ=255)
                                                                                                                                                                                         gTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCAGATTAGCTTTGAATTGGGTATCCGCGTGGAATTCCGGCACGAGGTAATGATAGTTGGTGTCAAACCATTTGGTCATTTCaa                                                >  1:432999/1‑251 (MQ=255)
                                                                                                                                                                                                 tttCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGTATCCGCGTGGAATTCCGGCACGAGGTAATGATAGTTGGTGTCAAACCATTTGGt                                                        <  1:849431/235‑1 (MQ=255)
                                                                                                                                                                                                      aCATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAg                                                                                                                           >  1:788211/1‑163 (MQ=255)
                                                                                                                                                                                                      aCATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGt                                                                                                              <  1:676412/176‑1 (MQ=255)
                                                                                                                                                                                                      aCATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGTATCCGCGTGGAATTCCGGCACGAGGTAATGATAGTTGGTGTCAAACCAtt                                                            >  1:56846/1‑226 (MQ=255)
                                                                                                                                                                                                      aCATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGTATCCGCGTGGAATTCCGGCACGAGGTAATGATAGTTGGTGTCAAACCATTTGGTCATTTCAATGGCAAATtgtg                                    >  1:642854/1‑250 (MQ=255)
                                                                                                                                                                                                      aCATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGTATCCGCGTGGAATTCCGGCACGAGGTAATGATAGTTGGTGTCAAACCATTTGGTCATTTCAATGGCAAATtgtg                                    >  1:710883/1‑250 (MQ=255)
                                                                                                                                                                                                      aCATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGAGGGTTTAACATTCAAGCCAAGGGCCTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGTATCCGCGTGGAATTCCGGCACGAGGTAATGATAGTTGGTGTCAAACCATTTGGTCATTTCAATGGCAAATtgtg                                    >  1:612866/1‑250 (MQ=255)
                                                                                                                                                                                                                    gAGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGc                                                                                                                                                                    <  1:146774/108‑1 (MQ=255)
                                                                                                                                                                                                                     aGTTTATTTAATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGTATCCGCGTGGAATTCCGGCACGAGGTAATGATAGTTGGTGTCAAACCATTTGGTCATTTCAATGGCAAATTGTGCTTTGTTACcgcgcg                     >  1:4621/1‑250 (MQ=255)
                                                                                                                                                                                                                        ttatttaATAAATCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGTATCCGCGTGGAATTCCGGCACGAGGTAATGATAGTTGGTGTCAAACCATTTGGTCATTTCAATGGCAAATTGTGCTTTGTTACCGCGCGCCAg                 <  1:780388/251‑1 (MQ=255)
                                                                                                                                                                                                                                  aaTCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGTATCCGCGTGGAATTCCGGCACGAGGTAATGATAGTTGGTGTCAAACCATTTGGTCATTTCAATGGCAAATTGTGCTTTGTTACCGCGCGCCAGTTGGAAatat       >  1:545618/1‑251 (MQ=255)
                                                                                                                                                                                                                                   aTCAAAGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAG‑ACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGTATCCGCGTGGAATTCCGGCACGAGGTAATGATAGTTGGTGTCAAACCATTTGGTCATTTCAATGGCAAATTGTGCTTTGTTACCGCGCGCCAGTTGGAAATATTg     <  1:610471/251‑1 (MQ=255)
                                                                                                                                                                                                                                       aaGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGTATCCGCGTGGAATTCCGGCACGAGGTAATGATAGTTGGTGTCAAACCATTTGGTCATTTCAATGGCAAATTGTGCTTTGTTACCGCGCGCCAGTTGGAAATATTGATc  <  1:668099/251‑1 (MQ=255)
                                                                                                                                                                                                                                       aaGCGATTAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGTATCCGCGTGGAATTCCGGCACGAGGTAATGATAGTTGGTGTCAAACCATTTGGTCATTTCAATGGCAAATTGTGCTTTGTTACCGCGCGCCAGTTGGAAATATTGATc  <  1:873217/251‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   
GCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATTTAATAAATCAAAGCGATCAAATGCCGCACCTTTTTCTTTGCCTAACCACAAGAACGTTAATGGACCAACAATAGTGGGTTTAACATTCAAGCCAAGGGCTTTGGCTTCGCGAATTTGTTGCACGTAATGCGCCGGATTAGCTTTGAATTGGGTATCCGCGTGGAATTCCGGCACGAGGTAATGATAGTTGGTGTCAAACCATTTGGTCATTTCAATGGCAAATTGTGCTTTGTTACCGCGCGCCAGTTGGAAATATTGATC  >  1/570747‑571228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑