Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	570,731	G→A	G258G (GGC→GGT)	unknown ←	5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine methyltransferase (EC 2.1.1.14)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	570,731	0	G	A	100.0%	191.3 / NA	71	G258G (GGC→GGT)	unknown	5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine methyltransferase (EC 2.1.1.14)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/34);  total (37/34)

GTTTACTTGTAACTGTGTTTCCACCGATAAATCATAAGGGGTGTGAAGCAGAGAACAGCTTGGCGCAATCCACAAACGATCGCCCAGTTTCGCTTTTAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAGCCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATT  >  1/570494‑570967                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             gtttACTTGTAACTGTGTTTCCACCGATAAATCATAAGGGGTGTGAAGCAGAGAACAGCTTGGCGCAATCCACAAACGATCGCCCAGTTTCGCTTTTAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCa                                                                                                                                                                                                                                 >  1:103391/1‑251 (MQ=255) gtttACTTGTAACTGTGTTTCCACCGATAAATCATAAGGGGTGTGAAGCAGAGAACAGCTTGGCGCAATCCACAAACGATCGCCCAGTTTCGCTTTTAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCa                                                                                                                                                                                                                                 >  1:877018/1‑251 (MQ=255) gtttACTTGTAACTGTGTTTCCACCGATAAATCATAAGGGGTGTGAAGCAGAGAACAGCTTGGCGCAATCCACAAACGATCGCCCAGTTTCGCTTTTAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCa                                                                                                                                                                                                                                 >  1:199014/1‑251 (MQ=255)      cTTGTAACTGTGTTTCCACCGATAAATCATAAGGGGTGTGAAGCAGAGAACAGCTTGGCGCAATCCACAAACGATCGCCCAGTTTCGCTTTTAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAgg                                                                                                                                                                                                                              >  1:580466/1‑249 (MQ=255)            aCTGTGTTTCCACCGATAAATCATAAGGGGTGTGAAGCAGAGAACAGCTTGGCGCAATCCACAAACGATCGCCCAGTTTCGCTTTTAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTtaat                                                                                                                                                                                                                      <  1:688414/251‑1 (MQ=255)               gtgtTTCCACCGATAAATCATAAGGGGTGTGAAGCAGAGAACAGCTTGGCGCAATCCACAAACGATCGCCCAGTTTCGCTTTTAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTtaataa                                                                                                                                                                                                                    >  1:110053/1‑250 (MQ=255)                               aTCATAAGGGGTGTGAAGCAGAGAACAGCTTGGCGCAATCCACAAACGATCGCCCAGTTTCGCTTTTAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAAccc                                                                                                                                                                                                                                           <  1:261472/211‑1 (MQ=255)                               aTCATAAGGGGTGTGAAGCAGAGAACAGCTTGGCGCAATCCACAAACGATCGCCCAGTTTCGCTTTTAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGc                                                                                                                                                                                                                             <  1:869785/225‑1 (MQ=255)                                 cATAAGGGGTGTGAAGCAGAGAACAGCTTGGCGCAATCCACAAACGATCGCCCAGTTTCGCTTTTAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGCAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACa                                                                                                                                                                                                 >  1:359492/1‑251 (MQ=255)                                 cATAAGGGGTGTGAAGCAGAGAACAGCTTGGCGCAATCCACAAACGATCGCCCAGTTTCGCTTTTAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACa                                                                                                                                                                                                 >  1:393899/1‑251 (MQ=255)                                        ggTGTGAAGCAGAGAACAGCTTGGCGCAATCCACAAACGATCGCCCAGTTTCGCTTTTAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTtaat                                                                                                                                                                                                                      <  1:569599/223‑1 (MQ=255)                                         gtgtGAAGCAGAGAACAGCTTGGCGCAATCCACAAACGATCGCCCAGTTTCGCTTTTAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGaa                                                                                                                                                                                         >  1:211174/1‑251 (MQ=255)                                         gtgtGAAGCAGAGAACAGCTTGGCGCAATCCACAAACGATCGCCCAGTTTCGCTTTTAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGaa                                                                                                                                                                                         >  1:523944/1‑251 (MQ=255)                                                gCAGAGAACAGCTTGGCGCAATCCACAAACGATCGCCCAGTTTCGCTTTTAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGaaa                                                                                                                                                                                        <  1:303598/245‑1 (MQ=255)                                                gCAGAGAACAGCTTGGCGCAATCCACAAACGATCGCCCAGTTTCGCTTTTAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTg                                                                                                                                                                                  >  1:265798/1‑251 (MQ=255)                                                   gagaACAGCTTGGCGCAATCCACAAACGATCGCCCAGTTTCGCTTTTAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTtaat                                                                                                                                                                                                                      >  1:826911/1‑212 (MQ=255)                                                          gCTTGGCGCAATCCACAAACGATCGCCCAGTTTCGCTTTTAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGAc                                                                                                                                                                                                  >  1:226289/1‑225 (MQ=255)                                                             tGGCGCAATCCACAAACGATCGCCCAGTTTCGCTTTTAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAAtt                                                                                                                                                                     <  1:227167/251‑1 (MQ=255)                                                                    aTCCACAAACGATCGCCCAGTTTCGCTTTTAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTtaataa                                                                                                                                                                                                                    <  1:326878/197‑1 (MQ=255)                                                                        acaAACGATCGCCCAGTTTCGCTTTTAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTa                                                                                                                                                            >  1:799244/1‑249 (MQ=255)                                                                           aaCGATCGCCCAGTTTCGCTTTTAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTg                                                                                                                                                                                  <  1:309706/224‑1 (MQ=255)                                                                               aTCGCCCAGTTTCGCTTTTAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGc                                                                                                                                                   <  1:133900/251‑1 (MQ=255)                                                                                       gTTTCGCTTTTAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAAtgtg                                                                                                                                           >  1:485807/1‑251 (MQ=255)                                                                                        tttCGCTTTTAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAAtg                                                                                                                                             <  1:381506/248‑1 (MQ=255)                                                                                               tttAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTacac                                                                                                                                   <  1:647462/251‑1 (MQ=255)                                                                                               tttAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTacac                                                                                                                                   <  1:782697/251‑1 (MQ=255)                                                                                                  aaCGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTc                                                                                                                                <  1:45731/251‑1 (MQ=255)                                                                                                          caccacATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAg                                                                                                                        >  1:769510/1‑251 (MQ=255)                                                                                                              acaTCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCg                                                                                                                     >  1:557852/1‑250 (MQ=255)                                                                                                               caTCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCa                                                                                                                   >  1:372689/1‑251 (MQ=255)                                                                                                                 tCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAg                                                                                                                                                                          >  1:286386/1‑194 (MQ=255)                                                                                                                      cacTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCAtt                                                                                                            >  1:502131/1‑251 (MQ=255)                                                                                                                       acTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCAttt                                                                                                           >  1:564697/1‑251 (MQ=255)                                                                                                                          tGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTc                                                                                                                                <  1:180688/227‑1 (MQ=255)                                                                                                                          tGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAACCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGc                                                                                                        <  1:665852/251‑1 (MQ=255)                                                                                                                            gTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTtgtg                                                                                                                                                      >  1:739707/1‑203 (MQ=255)                                                                                                                            gTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCg                                                                                                      >  1:628390/1‑251 (MQ=255)                                                                                                                            gTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCg                                                                                                      >  1:552272/1‑251 (MQ=255)                                                                                                                                        aCGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAg                                                                                          <  1:660849/251‑1 (MQ=255)                                                                                                                                         cGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGt                                                                                         >  1:116914/1‑251 (MQ=255)                                                                                                                                               aTGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAg                                                                                    >  1:97424/1‑250 (MQ=255)                                                                                                                                                tGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTtata                                                                                                                          >  1:881512/1‑211 (MQ=255)                                                                                                                                                       atcATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGGACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGt                                                                           >  1:707332/3‑251 (MQ=255)                                                                                                                                                       aCCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTtgtg                                                                                                                                                      >  1:788288/1‑176 (MQ=255)                                                                                                                                                         cATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGc                                                                                   >  1:487503/1‑241 (MQ=255)                                                                                                                                                         cATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCgcca                                                                         >  1:743091/1‑248 (MQ=255)                                                                                                                                                         cATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCa                                                                         <  1:133468/251‑1 (MQ=255)                                                                                                                                                            caatcaCGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAAtgt                                                                                                                                            <  1:264851/181‑1 (MQ=255)                                                                                                                                                                    cccACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTtat                                                                                                                           <  1:855492/190‑1 (MQ=255)                                                                                                                                                                          gACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCg                                                                                                                                                                >  1:375367/1‑147 (MQ=255)                                                                                                                                                                          gACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCa                                                         >  1:382232/1‑250 (MQ=255)                                                                                                                                                                           aCAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATcc                                                          <  1:504697/248‑1 (MQ=255)                                                                                                                                                                           aCAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATcc                                                          <  1:836694/248‑1 (MQ=255)                                                                                                                                                                           aCAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATcc                                                          <  1:774190/248‑1 (MQ=255)                                                                                                                                                                           aCAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATcc                                                          <  1:359047/248‑1 (MQ=255)                                                                                                                                                                           aCAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATc                                                           <  1:24278/247‑1 (MQ=255)                                                                                                                                                                                aTCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATc                                                           <  1:169628/242‑1 (MQ=255)                                                                                                                                                                                                    ggCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTc                                                  <  1:868265/231‑1 (MQ=255)                                                                                                                                                                                                      cGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGAt                             <  1:199245/250‑1 (MQ=255)                                                                                                                                                                                                          aaGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTa                        >  1:886050/1‑251 (MQ=255)                                                                                                                                                                                                             ttGGTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACAt                     <  1:51066/251‑1 (MQ=255)                                                                                                                                                                                                               ggTCAGGCGCACGCACTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTAc                       <  1:372816/247‑1 (MQ=255)                                                                                                                                                                                                                          cgcacTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAg        <  1:749777/251‑1 (MQ=255)                                                                                                                                                                                                                            cacTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCa                                                                                  >  1:413670/1‑175 (MQ=255)                                                                                                                                                                                                                            cacTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGtt      >  1:669209/1‑251 (MQ=255)                                                                                                                                                                                                                            cacTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGtt      >  1:567899/1‑251 (MQ=255)                                                                                                                                                                                                                              cTAAATCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGttta    >  1:422791/1‑251 (MQ=255)                                                                                                                                                                                                                                aaaTCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAatat                                                               <  1:531848/190‑1 (MQ=255)                                                                                                                                                                                                                                aaaTCAATGTGTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGtttatt  <  1:541950/251‑1 (MQ=255)                                                                                                                                                                                                                                       tgtgTAAACCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAAc                                                                                                                                                                                             <  1:713183/57‑1 (MQ=255)                                                                                                                                                                                                                                        gtgtAAACCCGCAGCCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGc                                                                                   <  1:413012/162‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             GTTTACTTGTAACTGTGTTTCCACCGATAAATCATAAGGGGTGTGAAGCAGAGAACAGCTTGGCGCAATCCACAAACGATCGCCCAGTTTCGCTTTTAACGGTTCCACCACATCCAACACTTGGTTCAGGTTCGCACGCCAAATGTTACGACCATCAATCACGCCCACAGACAAAATCTTGTCGTAATCAGCAAAGGCGGAAAGTTGGTCAGGCGCACGCACTAAATCAATGTGTAAGCCCGCAACCGGCAAGGCTTTTAATAACGCAGCGTGTTCCGCGACAGAACCGAAATAAGTGGCTAACAGTAATTTTGCGTTTACTTGTGTGCTTAATGTGGCGTACACGTCTTTATAAGCCGCAACCCATTTAGCCGGCAAATCGACAGTTAAAGCAGGTTCGTCAATTTGAATATAATCCACGCCTTCTGCTGCCAATGCGTTTAAGATTTCTACATAAACCGGTATGAGTTTATT  >  1/570494‑570967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑