Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	563,213	C→T	R270K (AGG→AAG)	unknown ←	Manganese ABC transporter, periplasmic‑binding protein SitA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	563,213	0	C	T	100.0%	205.1 / NA	75	R270K (AGG→AAG)	unknown	Manganese ABC transporter, periplasmic‑binding protein SitA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/40);  total (35/40)

TCAGACTTTTAAACAGGGTTGACTTACCGCTCCCGTTCACGCCGACTAAAGCGCAGGTCGTACCGCCTTCTAGTTTAAAAGAAACATCATGAATGGCGGTATGACCATTGTTGTAACGAACGACTACGTCTTCAACGCAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCCTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTAATCGGCCACAAATAAGCCTCTTTAAAACCGTAATCACGTGCTAAATAACTGAA  >  1/562978‑563448                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                            tCAGACTTTTAAACAGGGTTGACTTACCGCTCCCGTTCACGCCGACTAAAGCGCAGGTCGTACCGCCTTCTAGTTTAAAAGAAACATCATGAATGGCGGTATGACCATTGTTGTAACGAACGACTACGTCTTCAACGCAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAagag                                                                                                                                                                                                                              <  1:561679/251‑1 (MQ=255)     aCTTTTAAACAGGGTTGACTTACCGCTCCCGTTCACGCCGACTAAAGCGCAGGTCGTACCGCCTTCTAGTTTAAAAGAAACATCATGAATGGCGGTATGACCATTGTTGTAACGAACGACTACGTCTTCAACGCAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAagagag                                                                                                                                                                                                                            <  1:641701/249‑1 (MQ=255)                gggTTGACTTACCGCTCCCGTTCACGCCGACTAAAGCGCAGGTCGTACCGCCTTCTAGTTTAAAAGAAACATCATGAATGGCGGTATGACCATTGTTGTAACGAACGACTACGTCTTCAACGCAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCa                                                                                                                                                                                                               >  1:764651/1‑251 (MQ=255)                 ggTTGACTTACCGCTCCCGTTCACGCCGACTAAAGCGCAGGTCGTACCGCCTTCTAGTTTAAAAGAAACATCATGAATGGCGGTATGACCATTGTTGTAACGAACGACTACGTCTTCAACGCAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAagagag                                                                                                                                                                                                                            <  1:40029/237‑1 (MQ=255)                 ggTTGACTTACCGCTCCCGTTCACGCCGACTAAAGCGCAGGTCGTACCGCCTTCTAGTTTAAAAGAAACATCATGAATGGCGGTATGACCATTGTTGTAACGAACGACTACGTCTTCAACGCAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAg                                                                                                                                                                                                                 <  1:391112/248‑1 (MQ=255)                 ggTTGACTTACCGCTCCCGTTCACGCCGACTAAAGCGCAGGTCGTACCGCCTTCTAGTTTAAAAGAAACATCATGAATGGCGGTATGACCATTGTTGTAACGAACGACTACGTCTTCAACGCAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGc                                                                                                                                                                                                                <  1:550346/249‑1 (MQ=255)                 ggTTGACTTACCGCTCCCGTTCACGCCGACTAAAGCGCAGGTCGTACCGCCTTCTAGTTTAAAAGAAACATCATGAATGGCGGTATGACCATTGTTGTAACGAACGACTACGTCTTCAACGCAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGc                                                                                                                                                                                                                <  1:43401/249‑1 (MQ=255)                 ggTTGACTTACCGCTCCCGTTCACGCCGACTAAAGCGCAGGTCGTACCGCCTTCTAGTTTAAAAGAAACATCATGAATGGCGGTATGACCATTGTTGTAACGAACGACTACGTCTTCAACGCAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGc                                                                                                                                                                                                                <  1:363786/249‑1 (MQ=255)                        ttACCGCTCCCGTTCACGCCGACTAAAGCGCAGGTCGTACCGCCTTCTAGTTTAAAAGAAACATCATGAATGGCGGTATGACCATTGTTGTAACGAACGACTACGTCTTCAACGCAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCg                                                                                                                                                                                                       <  1:652099/251‑1 (MQ=255)                                        cGCCGACTAAAGCGCAGGTCGTACCGCCTTCTAGTTTAAAAGAAACATCATGAATGGCGGTATGACCATTGTTGTAACGAACGACTACGTCTTCAACGCAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGttt                                                                                                                                                                                       >  1:533105/1‑251 (MQ=255)                                             aCTAAAGCGCAGGTCGTACCGCCTTCTAGTTTAAAAGAAACATCATGAATGGCGGTATGACCATTGTTGTAACGAACGACTACGTCTTCAACGCAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCttt                                                                                                                                                                                   <  1:279117/250‑1 (MQ=255)                                              cTAAAGCGCAGGTCGTACCGCCTTCTAGTTTAAAAGAAACATCATGAATGGCGGTATGACCATTGTTGTAACGAACGACTACGTCTTCAACGCAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTg                                                                                                                                                                                 >  1:723341/1‑251 (MQ=255)                                                                 gCCTTCTAGTTTAAAAGAAACATCATGAATGGCGGTATGACCATTGTTGTAACGAACGACTACGTCTTCAACGCAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGttt                                                                                                                                                              >  1:728960/1‑251 (MQ=255)                                                                 gCCTTCTAGTTTAAAAGAAACATCATGAATGGCGGTATGACCATTGTTGTAACGAACGACTACGTCTTCAACGCAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGttt                                                                                                                                                              >  1:616215/1‑251 (MQ=255)                                                                                       tcatGAATGGCGGTATGACCATTGTTGTAACGAACGACTACGTCTTCAACGCAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTg                                                                                                                                        <  1:489942/251‑1 (MQ=255)                                                                                         atGAATGGCGGTATGACCATTGTTGTAACGAACGACTACGTCTTCAACGCAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGaa                                                                                                                                      >  1:324164/1‑251 (MQ=255)                                                                                                   gTATGACCATTGTTGTAACGAACGACTACGTCTTCAACGCAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGc                                                                                                                                                                                                                                         >  1:86717/1‑142 (MQ=255)                                                                                                    tATGACCATTGTTGTAACGAACGACTACGTCTTCAACGCAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCttt                                                                                                                                                                                   >  1:351963/1‑195 (MQ=255)                                                                                                          cATTGTTGTAACGAACGACTACGTCTTCAACGCAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATAttgt                                                                                                                     <  1:670522/251‑1 (MQ=255)                                                                                                            ttgttgTAACGAACGACTACGTCTTCAACGCAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTAttt                                                                                                                                                                                                  <  1:703436/172‑1 (MQ=255)                                                                                                              gttgtAACGAACGACTACGTCTTCAACGCAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCAc                                                                                                                                          >  1:140609/1‑226 (MQ=255)                                                                                                              gttgtAACGAACGACTACGTCTTCAACGCAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATAttgt                                                                                                                     <  1:805722/247‑1 (MQ=255)                                                                                                                                gTCTTCAACGCAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTa                                                                                                >  1:686199/1‑250 (MQ=255)                                                                                                                                  cTTCAACGCAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACtt                                                                                             <  1:161914/251‑1 (MQ=255)                                                                                                                                       aCGCAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACg                                                                                         >  1:889146/1‑250 (MQ=255)                                                                                                                                          cAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCgg                                                                                                                             >  1:9475/1‑211 (MQ=255)                                                                                                                                                      atatTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACg                                                                                                          >  1:768428/1‑218 (MQ=255)                                                                                                                                                       tatTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGTCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTAGCCGGTTTCGGTGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTg                                                                         >  1:77520/1‑250 (MQ=255)                                                                                                                                                        atTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTgcggcg                                                                           >  1:157482/1‑247 (MQ=255)                                                                                                                                                             atgaCATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCGCTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCttgt                                                                   <  1:528709/250‑1 (MQ=255)                                                                                                                                                                 cATTATTTTTCAAATCCTTTAGCAATTGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTGCTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTc                                                              <  1:751868/251‑1 (MQ=255)                                                                                                                                                                 cATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTc                                                              <  1:676995/251‑1 (MQ=255)                                                                                                                                                                        tttCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTa                                                       >  1:469926/1‑251 (MQ=255)                                                                                                                                                                           cAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTAATc                                                    <  1:285362/251‑1 (MQ=255)                                                                                                                                                                               tCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATa                                                                                                                                    <  1:476398/167‑1 (MQ=255)                                                                                                                                                                               tCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAAtt                                                                                                 <  1:274541/202‑1 (MQ=255)                                                                                                                                                                               tCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTgcgg                                                                             >  1:843866/1‑222 (MQ=255)                                                                                                                                                                                 cTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTAATCGGCcac                                              <  1:180899/251‑1 (MQ=255)                                                                                                                                                                                         aTGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAat                                                                                                                          <  1:749576/167‑1 (MQ=255)                                                                                                                                                                                         aTGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGc                                                                                        <  1:567185/201‑1 (MQ=255)                                                                                                                                                                                           ggTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGc                                                                                        <  1:465592/199‑1 (MQ=255)                                                                                                                                                                                           ggTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCttgt                                                                   <  1:489237/220‑1 (MQ=255)                                                                                                                                                                                           ggTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTAATCGGCCACAAATAAGc                                      >  1:167203/1‑249 (MQ=255)                                                                                                                                                                                           ggTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTAATCGGCCACAAATAAGc                                      >  1:37752/1‑249 (MQ=255)                                                                                                                                                                                            gTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATAttg                                                                                                                      >  1:250253/1‑168 (MQ=255)                                                                                                                                                                                            gTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGAc                                                                                                           <  1:283102/179‑1 (MQ=255)                                                                                                                                                                                                      tCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTAATCGGCCACAAATAAGCCTCTTTAAAAc                           <  1:494614/249‑1 (MQ=255)                                                                                                                                                                                                      tCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTAATCGGCCACAAATAAGCCTCTTTAAAAc                           <  1:384644/249‑1 (MQ=255)                                                                                                                                                                                                         cTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTAAt                                                     <  1:354451/220‑1 (MQ=255)                                                                                                                                                                                                         cTTTTAATAAATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTAATCGGCCACAAATAAGCCTCTTTAAAACCGTaa                      >  1:448651/1‑251 (MQ=255)                                                                                                                                                                                                             taataaATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTAttt                                                                                                                                                                                                  <  1:376063/75‑1 (MQ=255)                                                                                                                                                                                                             taataaATCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTAATCGGCCACAAATAAGCCTCTTTAAAAc                           >  1:389609/1‑242 (MQ=255)                                                                                                                                                                                                                  aaTCAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAAtt                                                                                                 >  1:346259/1‑167 (MQ=255)                                                                                                                                                                                                                     cAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAAtt                                                                                                 >  1:671511/1‑164 (MQ=255)                                                                                                                                                                                                                     cAATATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTCCGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTAATCGGCCACAAATAAGCCTCTTTAAAACCGTAATCACGTGCTaaa          >  1:444954/1‑251 (MQ=255)                                                                                                                                                                                                                      aaTATAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTAATCGGCCACAAATAAGCCTCTTTAAAACCGTAATCACGTGCTAAAt         <  1:871073/251‑1 (MQ=255)                                                                                                                                                                                                                       atatAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACttgttg                                                                                <  1:308657/179‑1 (MQ=255)                                                                                                                                                                                                                       atatAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCttgt                                                                   >  1:875488/1‑192 (MQ=255)                                                                                                                                                                                                                       atatAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTc                                                              <  1:306421/197‑1 (MQ=255)                                                                                                                                                                                                                       atatAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTAATCGGCCACAAATAAGCCTCTTTAAAAc                           <  1:746662/232‑1 (MQ=255)                                                                                                                                                                                                                       atatAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTAATCGGCCACAAATAAGCCTCTTTAAAACCGTAATCACGTGCTAAATa        >  1:385819/1‑251 (MQ=255)                                                                                                                                                                                                                       atatAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTAATCGGCCACAAATAAGCCTCTTTAAAACCGTAATCACGTGCTAAATa        >  1:143081/1‑251 (MQ=255)                                                                                                                                                                                                                       atatAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTAATCGGCCACAAATAAGCCTCTTTAAAACCGTAATCACGTGCTAAATa        >  1:634415/1‑251 (MQ=255)                                                                                                                                                                                                                       atatAAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTCGCTCAGCGTTAATCGGCCACAAATAAGCCTCTTTAAAACCGTAATCACGTGCTAAATa        >  1:805189/1‑251 (MQ=255)                                                                                                                                                                                                                         ataAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTAATCGGCCACAAATAAGCCTCTTTAAAACCGTAATCACGTGCTAAATAAc      <  1:509086/251‑1 (MQ=255)                                                                                                                                                                                                                         ataAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTAATCGGCCACAAATAAGCCTCTTTAAAACCGTAATCACGTGCTAAATAAc      <  1:873246/251‑1 (MQ=255)                                                                                                                                                                                                                         ataAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTAATCGGCCACAAATAAGCCTCTTTAAAACCGTAATCACGTGCTAAATAAc      <  1:30630/251‑1 (MQ=255)                                                                                                                                                                                                                         ataAGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTAATCGGCCACAAATAAGCCTATTTAAAACCGTAATCACGTGCTAAATAAc      <  1:632531/251‑1 (MQ=255)                                                                                                                                                                                                                            aGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTAATCGGCCACAAATAAGCCTCTTTAAAACCGTAATCACGTGCTAAATAACTGa   >  1:633497/1‑251 (MQ=255)                                                                                                                                                                                                                            aGTCGGCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTAATCGGCCACAAATAAGCCTCTTTAAAACCGTAATCACGTGCTAAAAAACTGa   >  1:449638/1‑251 (MQ=255)                                                                                                                                                                                                                                 gCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTAATc                                                    >  1:419734/1‑197 (MQ=255)                                                                                                                                                                                                                                 gCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTAATCGGCCACAAATAAGCCTCTTTAAAAc                           <  1:417298/222‑1 (MQ=255)                                                                                                                                                                                                                                 gCACAGGACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTAATCGGCCACAAATAAGCCTCTTTAAAACCGTAATCACGTGCTAAATAACTGaa  <  1:795728/247‑1 (MQ=255)                                                                                                                                                                                                                                      ggACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATAttg                                                                                                                      >  1:610740/1‑126 (MQ=255)                                                                                                                                                                                                                                      ggACCCTTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTAATCGGCCACAAATAAGCCTCTTTAAAAc                           >  1:703065/1‑217 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                            TCAGACTTTTAAACAGGGTTGACTTACCGCTCCCGTTCACGCCGACTAAAGCGCAGGTCGTACCGCCTTCTAGTTTAAAAGAAACATCATGAATGGCGGTATGACCATTGTTGTAACGAACGACTACGTCTTCAACGCAAATAGAGGCAATATTTGATGACATTATTTTTCAAATCCTTTAGCAATGGTAGAAACAGTCACTTTTAATAAATCAATATAAGTCGGCACAGGACCCCTGGCACCGGAAAGAGAGTCAACATAAAGCACGCCACCGTATTTTGCACCGGTTTCTTTTGCTACTTGTTTTGCCGGTTTCGGGGAGATGGTGCTTTCACTGAATACGACCGGAATATTGTTGGCTTTGACGGTTTCAATTACTTTACGCACTTGTTGCGGCGTGCCTTGTTGCTCAGCGTTAATCGGCCACAAATAAGCCTCTTTAAAACCGTAATCACGTGCTAAATAACTGAA  >  1/562978‑563448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑