Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	550,373	C→A	L207I (CTT→ATT)	unknown →	FIG00781545: hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	550,373	0	C	A	100.0%	55.3 / NA	23	L207I (CTT→ATT)	unknown	FIG00781545: hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/11);  total (12/11)

TTTTTACTTCTATATAGGATTTTGAAATTCATTTATGAAAATGGTGAGTTAAATAATAAAAATAATTATTCAAGTTTATTGAGAAGTTTTATATCTAATAAATTATTAGTTGTATTGTCTTATCATCTATCTAATAGAGATGACTCTTATAGGAATTATATTGAATATATTAATAGGTTCTCATTTTTGGAGCACGTAAATTTTATAGATCTAGAACTTGAATTTATACATCAAGTGAGTAGGGTAGAGAAAGAAGCTTTATACGATTTAGTTTTTAAGGGAGAAAGTTATCTATTTGATGCTCTTAAGAGTGGTGAATTAATTCCTGATGATATTGATAGCTATGATCGTGAAATGTTTTTAAAGGAAATTATCCAAAGTAAACAGGAAAATTGTCCTATAATATATAAAAAGTTTATGGGGTTAAATTTGGGATATGTCAAGGAAGGGAAATC  >  1/550157‑550611                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               tttttACTTCTATATAGGATTTTGAAATTCATTTATGAAAATGGTGAGTTAAATAATAAAAATAATTATTCAAGTTTATTGAGAAGTTTTATATCTAATAAATTATTAGTTGTATTGTCTTATCATCTATCTAATAGAGATGACTCTTATAGGAATTATATTGAATATATTAATAGGTTCTCATTTTTGGAGCACGTAAATTTTATAGATCTAGAAATTGAATTTATACATCAAGTGAGTAGGGTAGagaa                                                                                                                                                                                                              <  1:620099/251‑1 (MQ=255)                                              gAGTTAAATAATAAAAATAATTATTCAAGTTTATTGAGAAGTTTTATATCTAATAAATTATTAGTTGTATTGTCTTATCATCTATCTAATAGAGATGACTCTTATAGGAATTATATTGAATATATTAATAGGTTCTCATTTTTGGAGCACGTAAATTTTATAGATCTAGAAATTGAATTTATACATCAAGTGAGGAGGGTAGAGAAAGAAGCGTTATACGATTTAGTTTTTAAGGGAGAAAgg                                                                                                                                                                         >  1:319961/1‑242 (MQ=255)                                                             aataatTATTCAAGTTTATTGAGAAGTTTTATATCTAATAAATTATTAGTTGTATTGTCTTATCATCTATCTAATAGAGATGACTCTTATAGGAATTATATTGAATATATTAATAGGTTCTCATTTTTGGAGCACGTAAATTTTATAGATCTAGAAATTGAATTTATACATCAAGTGAGTAGGGTAGAGAAAGAAGCTTTATACGATTTAGTTTTTAAGGGAGAAAGTTATCTATTTGATGCTCTTAagag                                                                                                                                                  <  1:233932/251‑1 (MQ=255)                                                                                      gTTTTATATCTAATAAATTATTAGTTGTATTGTCTTATCATCTATCTAATAGAGATGACTCTTATAGGAATTATATTGAATATATTAATAGGTTCTCATTTTTGGAGCACGTAAATTTTATAGATCTAGAAATTGAATTTATACATCAAGTGAGTAGGGTAGAGAAAGAAGCTTTATACGATTTAGTTTTTAAGGGAGAAAGTTATCTATTTGATGCTCTTAAGAGTGGTGAATTAATTCCTGATGATAtt                                                                                                                         >  1:107305/1‑251 (MQ=255)                                                                                               cTAATAAATTATTAGTTGTATTGTCTTATCATCTATCTAATAGAGATGACTCTTATAGGAATTATATTGAATATATTAATAGGTTCTCATTTTTGGAGCACGTAAATTTTATAGATCTAGAAATTGAATTTATACATCAAGTGAGTAGGGTAGAGAAAGAAGCTTTATAc                                                                                                                                                                                                 <  1:482047/170‑1 (MQ=255)                                                                                                                                 atctaATAGAGATGACTCTTATAGGAATTATATTGAATATATTAATAGGTTCTCATTTTTGGAGCACGTAAATTTTATAGATCTAGAAATTGAATTTATACATCAAGTGAGTAGGGTAGAGAAAGAAGCTTTATACGATTTAGTTTTTAAGGGAGAAAGTTATCTATTTGATGCTCTTAagag                                                                                                                                                  >  1:657039/1‑183 (MQ=255)                                                                                                                                          agaTGACTCTTATAGGAATTATATTGAATATATTAATAGGTTCTCATTTTTGGAGCACGTAAATTTTATAGATCTAGAAATTGAATTTATACATCAAGTGAGTAGGGTAGAGAAAGAAGCTTTATACGATTTAGTTTTTAAGGGAGAAAGTTATCTATTTGATGCTCTTAAGAGTGGTGAATTAATTCCTGATGATATTGATAGCTa                                                                                                                 <  1:497814/207‑1 (MQ=255)                                                                                                                                                ctctTATAGGAATTATATTGAATATATTAATAGGTTCTCATTTTTGGAGCACGTAAATTTTATAGATCTAGAAATTGAATTTATACATCAAGTGAGTAGGGTAGAGAAAGAAGCTTTATACGATTTAGTTTTTAAGGGAGAAAGTTATCTATTTGATGCTCTTAAGAGTGGTGAATTAATTc                                                                                                                                    >  1:674003/1‑182 (MQ=255)                                                                                                                                                ctctTATAGGAATTATATTGAATATATTAATAGGTTCTCATTTTTGGAGCACGTAAATTTTATAGATCTAGAAATTGAATTTATACATCAAGTGAGTAGGGTAGAGAAAGAAGCTTTATACGATTTAGTTTTTAAGGGAGAAAGTTATCTATTTGATGCTCTTAAGAGTGGTGAATTAATTCCTGATGATATTGATAGCTATGATCg                                                                                                           >  1:179127/1‑207 (MQ=255)                                                                                                                                                         gAATTATATTGAATATATTAATAGGTTCTCATTTTTGGAGCACGTAAATTTTATAGATCTAGAAATTGAATTTATACATCAAGTGAGTAGGGTAGAGAAAGAAGCTTtata                                                                                                                                                                                                  >  1:88858/1‑111 (MQ=255)                                                                                                                                                         gAATTATATTGAATATATTAATAGGTTCTCATTTTTGGAGCACGTAAATTTTATAGATCTAGAAATTGAATTTATACATCAAGTGAGTAGGGTAGAGAAAGAAGCTTTATACGATTTAGTTTTTAAGGGAGAAAGTTATCTATTTGATGCTCTTAAGAGTGGTGAATTAATTCCTGATGATATTGATAGCTATg                                                                                                               >  1:198870/1‑194 (MQ=255)                                                                                                                                                         gAATTATATTGAATATATTAATAGGTTCTCATTTTTGGAGCACGTAAATTTTATAGATCTAGAAATTGAATTTATACATCAAGTGAGTAGGGTAGAGAAAGAAGCTTTATACGATTTAGTTTTTAAGGGAGAAAGTTATCTATTTGATGCTCTTAAGAGTGGTGAATTAATTCCTGATGATATTGATAGCTATGATCGTGAAATGTTTTTAAAGGAAATTATCCAAAGTAAACAGGAAAATTGTcc                                                           <  1:706028/246‑1 (MQ=255)                                                                                                                                                            ttATATTGAATATATTAATAGGTTCTCATTTTTGGAGCACGTAAATTTTATAGATCTAGAAATTGAATTTATACATCAAGTGAGTAGGGTAGAGAAAGAAGCTTTATACGATTTAGTTTTTAAGGGAGAAAGTTATCTATTTGATGCTCTTAAGAGTGGTGAATTAATTCCTGATGATATTGATAGCTATGATCGTGAAATGTTTTTAAAGGAAATTATCCAAAGTAAACAGGAAAATTGTCCTATAatat                                                   <  1:695877/251‑1 (MQ=255)                                                                                                                                                              atatTGAATATATTAATAGGTTCTCATTTTTGGAGCACGTAAATTTTATAGATCTAGAAATTGAATTTATACATCAAGTGAGTAGGGTAGagaaa                                                                                                                                                                                                             <  1:490459/95‑1 (MQ=255)                                                                                                                                                                   gAATATATTAATAGGTTCTCATTTTTGGAGCACGTAAATTTTATAGATCTAGAAATTGAATTTATACATCAAGTGAGTAGGGTAGAGAATGAAGCTTTATACGATTTAGTTTTTAAGGGAGAAAGTTATCTATTTGATGCTCTTAAGAGTGGTGAATTAATTCCTGATGCTATTGATAGCTATGATCGTGAACTGTTTTTAAAGGCAATTATCCAAAGTAAACAGGAAAATTGTCCTATAATATATaaaaa                                            >  1:449089/1‑251 (MQ=255)                                                                                                                                                                   gAATATATTAATAGGTTCTCATTTTTGGAGCACGTAAATTTTATAGATCTAGAAATTGAATTTATACATCAAGTGAGTAGGGTAGAGAAAGAAGCTTTATACGATTTAGTTTTTAAggg                                                                                                                                                                                <  1:351771/119‑1 (MQ=255)                                                                                                                                                                   gAATATATTAATAGGTTCTCATTTTTGGAGCACGTAAATTTTATAGATCTAGAAATTGAATTTATACATCAAGTGAGTAGGGTAGAGAAAGAAGCTTTATACGATTTAGTTTTTAAGGGAGAAAGTTATCTATTTGATGCTCTTAAGAGTGGTGaattaa                                                                                                                                       >  1:470086/1‑160 (MQ=255)                                                                                                                                                                     atatatTAATAGGTTCTCATTTTTGGAGCACGTAAATTTTATAGATCTAGAAATTGAATTTATACATCAAGTGAGTAg                                                                                                                                                                                                                       <  1:783170/78‑1 (MQ=255)                                                                                                                                                                        tatTAATAGGTTCTCATTTTTGGAGCACGTAAATTTTATAGATCTAGAAATTGAATTTATACATCAAGTGAGTAGGGTAGAGAAAGAAGCTTTATACGATTTAGTTTTTAAGGGAGAAAGt                                                                                                                                                                         >  1:677588/1‑121 (MQ=255)                                                                                                                                                                        tatTAATAGGTTCTCATTTTTGGAGCACGTAAATTTTATAGATCTAGAAATTGAATTTATACATCAAGTGAGTAGGGTAGAGAAAGAAGCTTTATACGATTTAGTTTTTAAGGGAGAAAGTTATCTATTTGATGCTCTTAagag                                                                                                                                                  >  1:320204/1‑144 (MQ=255)                                                                                                                                                                         atTAATAGGTTCTCATTTTTGGAGCACGTAAATTTTATAGATCTAGAAATTGAATTTATACATCAAGTGAGTAGGGTAGAGAAAGAAGCTTTATACGATTTAGTTTTTAAGGGAGAAAGTTATCTATTTGATGCTCTTAAGAGTGGTGAATTAATTCCTGATGATATTGATAGCTATGATCGTGAAATGTTTTTAAAGGAAATTATCCAAAGTAAACAGGAAAATTGTCCTataa                                                      <  1:760587/235‑1 (MQ=255)                                                                                                                                                                           tAATAGGTTCTCATTTTTGGAGCACGTAAATTTTATAGATCTAGAAATTGAATTTATACATCAAGTGAGTAGGGTAgaga                                                                                                                                                                                                               >  1:710591/1‑80 (MQ=255)                                                                                                                                                                                                             ataGATCTAGAAATTGAATTTATACATCAAGTGAGTAGGGTAGAGAAAGAAGCTTTATACGATTTAGTTTTTAAGGGAGAAAGTTATCTATTTGATGCTCTTAAGAGTGGTGAATTAATTCCTGATGATATTGATAGCTATGATCGTGAAATGTTTTTAAAGGAAATTATCCAAAGTAAACAGGAAAATTGTCCTATAATATATAAAAAGTTTATGGGGTTAAATTTGGGATATGTCAAGGAAGGGAAATc  <  1:230984/251‑1 (MQ=255)                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               TTTTTACTTCTATATAGGATTTTGAAATTCATTTATGAAAATGGTGAGTTAAATAATAAAAATAATTATTCAAGTTTATTGAGAAGTTTTATATCTAATAAATTATTAGTTGTATTGTCTTATCATCTATCTAATAGAGATGACTCTTATAGGAATTATATTGAATATATTAATAGGTTCTCATTTTTGGAGCACGTAAATTTTATAGATCTAGAACTTGAATTTATACATCAAGTGAGTAGGGTAGAGAAAGAAGCTTTATACGATTTAGTTTTTAAGGGAGAAAGTTATCTATTTGATGCTCTTAAGAGTGGTGAATTAATTCCTGATGATATTGATAGCTATGATCGTGAAATGTTTTTAAAGGAAATTATCCAAAGTAAACAGGAAAATTGTCCTATAATATATAAAAAGTTTATGGGGTTAAATTTGGGATATGTCAAGGAAGGGAAATC  >  1/550157‑550611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑