Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	544,832	A→G	intergenic (‑21/‑272)	unknown ← / → unknown	tRNA‑Leu‑CAA/UPF0299 membrane protein YohJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	544,832	0	A	G	100.0%	151.0 / NA	54	intergenic (‑21/‑272)	unknown/unknown	tRNA‑Leu‑CAA/UPF0299 membrane protein YohJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/35);  total (19/35)

TGTCGCATAATTGATAACGGCGCTAAAAGTGCGGTCATTTTTTCCGGTAAATTTTAAGGGAAGGGTTAACTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAAAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAATTCAACACATTCAATAATTTTGATCTATGTGAAATTTTTCCCTTACTTTCTTTTCTACTAGTGTAGTGGCTTTCCGGAAAAAATTCCCCTTCTTGTAAAATAAGAGTAGAATTATTTTAAATATTAAAAAAAAATTGAGTTTTCACTATG  >  1/544597‑545055                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                tGTCGCATAATTGATAACGGCGCTAAAAGTGCGGTCATTTTTTCCGGTAAATTTTAAGGGAAGGGTTAACTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGtattat                                                                                                                                                                                                                  <  1:313528/251‑1 (MQ=255)   tCGCATAATTGATAACGGCGCTAAAAGTGCGGTCATTTTTTCCGGTAAATTTTAAGGGAAGGGTTAACTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCGCCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATaa                                                                                                                                                                                                                <  1:685049/251‑1 (MQ=255)   tCGCATAATTGATAACGGCGCTAAAAGTGCGGTCATTTTTTCCGGTAAATTTTAAGGGAAGGGTTAACTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATaa                                                                                                                                                                                                                >  1:774367/1‑251 (MQ=255)   tCGCATAATTGATAACGGCGCTAAAAGTGCGGTCATTTTTTCCGGTAAATTTTAAGGGAAGGGTTAACTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATaa                                                                                                                                                                                                                <  1:747548/251‑1 (MQ=255)   tCGCATAATTGATAACGGCGCTAAAAGTGCGGTCATTTTTTCCGGTAAATTTTAAGGGAAGGGTTAACTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATaa                                                                                                                                                                                                                <  1:842895/251‑1 (MQ=255)   tCGCATAATTGATAACGGCGCTAAAAGTGCGGTCATTTTTTCCGGTAAATTTTAAGGGAAGGGTTAACTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATaa                                                                                                                                                                                                                <  1:515406/251‑1 (MQ=255)   tCGCATAATTGATAACGGCGCTAAAAGTGCGGTCATTTTTTCCGGTAAATTTTAAGGGAAGGGTTAACTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATaa                                                                                                                                                                                                                <  1:862610/251‑1 (MQ=255)   tCGCATAATTGATAACGGCGCTAAAAGTGCGGTCATTTTTTCCGGTAAATTTTAAGGGAAGGGTTAACTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATaa                                                                                                                                                                                                                <  1:864011/251‑1 (MQ=255)   tCGCATAATTGATAACGGCGCTAAAAGTGCGGTCATTTTTTCCGGTAAATTTTAAGGGAAGGGTTAACTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGACGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATaa                                                                                                                                                                                                                >  1:440152/1‑251 (MQ=255)    cGCATAATTGATAACGGCGCTAAAAGTGCGGTCATTTTTTCCGGTAAATTTTAAGGGAAGGGTTAACTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAg                                                                                                                                                                                                               <  1:551931/251‑1 (MQ=255)     gCATAATTGATAACGGCGCTAAAAGTGCGGTCATTTTTTCCGGTAAATTTTAAGGGAAGGGTTAACTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATaa                                                                                                                                                                                                                <  1:94979/249‑1 (MQ=255)        tAATTGATAACGGCGCTAAAAGTGCGGTCATTTTTTCCGGTAAATTTTAAGGGAAGGGTTAACTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGAt                                                                                                                                                                                                           <  1:478691/251‑1 (MQ=255)          aTTGATAACGGCGCTAAAAGTGCGGTCATTTTTTCCGGTAAATTTTAAGGGAAGGGTTAACTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATaa                                                                                                                                                                                                                <  1:879065/244‑1 (MQ=255)          aTTGATAACGGCGCTAAAAGTGCGGTCATTTTTTCCGGTAAATTTTAAGGGAAGGGTTAACTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTa                                                                                                                                                                                                         >  1:137592/1‑251 (MQ=255)              aTAACGGCGCTAAAAGTGCGGTCATTTTTTCCGGTAAATTTTAAGGGAAGGGTTAACTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGt                                                                                                                                                                                                      <  1:401532/250‑1 (MQ=255)                       cTAAAAGTGCGGTCATTTTTTCCGGTAAATTTTAAGGGAAGGGTTAACTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATaa                                                                                                                                                                                                                <  1:271128/231‑1 (MQ=255)                       cTAAAAGTGCGGTCATTTTTTCCGGTAAATTTTAAGGGAAGGGTTAACTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTgca                                                                                                                                                                                            >  1:885306/1‑251 (MQ=255)                                cGGTCATTTTTTCCGGTAAATTTTAAGGGAAGGGTTAACTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAAc                                                                                                                                                                                   <  1:6526/251‑1 (MQ=255)                                                  aaTTTTAAGGGAAGGGTTAACTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAAc                                                                                                                                                                 <  1:794802/251‑1 (MQ=255)                                                             aagggTTAACTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAAt                                                                                                                                                       <  1:327067/250‑1 (MQ=255)                                                               gggTTAACTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAAc                                                                                                                                                                                   >  1:309902/1‑220 (MQ=255)                                                               gggTTAACTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACCGCGGTTTTTGAATCCGCTGCGTCTACCAATTTTGCCACCCAAGCAGCATTGATAAGCTTCGTAAGAACGAGCGGAATTATAAggt                                                                                                                                                                                                             >  1:137241/1‑193 (MQ=255)                                                                ggTTAACTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTTGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCATTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAATTc                                                                                                                                                     <  1:452892/249‑1 (MQ=255)                                                                   tAACTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAATTCAacac                                                                                                                                                <  1:838628/251‑1 (MQ=255)                                                                   tAACTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAATTCAacac                                                                                                                                                <  1:646712/251‑1 (MQ=255)                                                                      cTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAAc                                                                                                                                                                 >  1:810687/1‑231 (MQ=255)                                                                                 aaaaaaaGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAAt                                                                                                                                                       <  1:348889/230‑1 (MQ=255)                                                                                    aaaaGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAATTCAACACATTCAATAATTTTGATc                                                                                                                               <  1:166784/251‑1 (MQ=255)                                                                                              ttttACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAATTCAACACATTCAATAATTTTGATCTATGTGAAAt                                                                                                                     >  1:580528/1‑251 (MQ=255)                                                                                                             aaTTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAATTCAACACATTCAATAATTTTGATCTATGTGAAATTTTTCCCTTActttc                                                                                                      <  1:172227/251‑1 (MQ=255)                                                                                                                  ttCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAAc                                                                                                                                                                                   <  1:701371/169‑1 (MQ=255)                                                                                                                               cATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAAt                                                                                                                                                       >  1:750442/1‑184 (MQ=255)                                                                                                                                aTTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAATTCAACACATTCAATAATTTTGATCTATGTGAAATTTTTCCCTTACTTTCTTTTCTACTAGTGTAGTgg                                                                                   <  1:881047/251‑1 (MQ=255)                                                                                                                                   ggTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATaa                                                                                                                                                                                                                >  1:54437/1‑123 (MQ=255)                                                                                                                                    gTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAATTCAACACATTCAATAATTTTGATCTATGTGAAATTTTTCCCTTACTTTCTTTTCTACtagtgtag                                                                                      <  1:255756/244‑1 (MQ=255)                                                                                                                                    gTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGAAAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAATTCAACACATTCAATAATTTTGATc                                                                                                                               >  1:837567/1‑203 (MQ=255)                                                                                                                                      gCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAATTCAACACATTCAATAATTTTGATCTATGTGAAATTTTTCCCTTACTTTCTTTTctac                                                                                              >  1:391722/1‑234 (MQ=255)                                                                                                                                      gCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAATTCAACACATTCAATAATTTTGATCTATGTGAAATTTTTCCCTTACTTTCTTTTctac                                                                                              >  1:3530/1‑234 (MQ=255)                                                                                                                                           gggTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAAt                                                                                                                                                       <  1:248953/172‑1 (MQ=255)                                                                                                                                            ggTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAATTCAACACATTCAATAATTTTGATCTATGTGAAATTTTTCCCTTACtttcttttct                                                                                                <  1:107852/226‑1 (MQ=255)                                                                                                                                                 gACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAATTCAACACATTCAATAAttt                                                                                                                                    >  1:644463/1‑185 (MQ=255)                                                                                                                                                 gACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAATTCAACACATTCAATAATTTTGATCTATGTGAAATTTTTCCCTTACTTTCTTTTCTACTAGTGTAGTGGCTTTCCGGAAAAAAtt                                                                   >  1:694399/1‑250 (MQ=255)                                                                                                                                                       aaCCGACACGTTTATTCAACGGCGGAATTTGAATTCGCCGCGTCTACCCATTTCGCCACCCAGGCAGCATTGATAAGCCTCGTAAGAACGAGTGGTATTCTAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAATTCAACACATTCAATAATTTTTATCTATGTGAAATTTTTCCCTTACTTTCTTTTCTACTAGTGTAGTGGCTTTCCGGCAAAAATTccccct                                                             >  1:576217/1‑248 (MQ=255)                                                                                                                                                               cGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAAt                                                                                                                                                       <  1:300465/152‑1 (MQ=255)                                                                                                                                                                     ttCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAATTCAACACATTCAATAATTTTGATCTATGTGAAATTTTTCCCTTACTTTCTTTTCTACTAGTGTAGTGGCTTTCCGGAAAAAATTCCCCTTCTTGTAAAATAagag                                              <  1:833617/251‑1 (MQ=255)                                                                                                                                                                            gcggATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAATTCAACACATTCAATAATTTTGATCTATGTGAAATTTTTCCCTTACTTTCTTTTCTACTAGTGTAGTGGCTTTCCGGAAAAAATTCCCCTTCTTGTAAAATAAGAGTAGAat                                        <  1:119714/250‑1 (MQ=255)                                                                                                                                                                                 ttttGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAATTCAACACATTCAATAATTTTGATCTATGTGAAATTTTTCCCTTACTTTCTTTTCTACTAGTGTAGTgg                                                                                   >  1:371304/1‑202 (MQ=255)                                                                                                                                                                                         ccGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAATTCAACACATTCAATAATTTTGATCTATGTGAAATTTTTCCCTTACTTTCTTTTCTACtagtg                                                                                         <  1:124128/188‑1 (MQ=255)                                                                                                                                                                                          cGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGt                                                                                                                                                                                                      <  1:702949/78‑1 (MQ=255)                                                                                                                                                                                                   tACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAATTCAACACATTCAATAATTTTGATCTATGTGAAATTTTTCCCTTACTTTCTTTTctac                                                                                              <  1:467190/173‑1 (MQ=255)                                                                                                                                                                                                                            ttGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAATTCAACACATTCAATAATTTTGATCTATGTGAAATTTTTCCCTTACTTTCTTTTCTACtagtgtag                                                                                      >  1:884466/1‑156 (MQ=255)                                                                                                                                                                                                                            ttGATAAGCTTCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAATTCAACACATTCAATAATTTTGATCTATGTGAAATTTTTCCCTTACTTTCTTTTCTACTAGTGTAGTGGCTTTCCGGAAAAAATTCCCCTTCTTGTAAAATAAGAGTAGAATTATTTTAAATATTAAAAAAAAGTTGAGTTTTCACTATg  >  1:901771/1‑240 (MQ=255)                                                                                                                                                                                                                                      tCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAATTCAACACATTCAATAATTTTGATc                                                                                                                               <  1:876316/105‑1 (MQ=255)                                                                                                                                                                                                                                      tCGTAAGAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAATTCAACACATTCAATAATTTTGATCTATGTGAAATTTTTCCCTTACTTTCTTTTCTACTAGTGTAGTGGCTTTCCGGAAAAAATTCCCCTTCTTGTAAAATAAGAGTAGAat                                        <  1:48023/192‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                TGTCGCATAATTGATAACGGCGCTAAAAGTGCGGTCATTTTTTCCGGTAAATTTTAAGGGAAGGGTTAACTTCAAGTGACAAAAAAAGCTGCGTTTTACGGCAGCTTTAATTTTTCAGAAACTTATCATTGGTGCCTAGGGTCGGACTCGAACCGACACGTTTATTCAACGGCGGATTTTGAATCCGCTGCGTCTACCAATTTCGCCACCCAGGCAGCATTGATAAGCTTCGTAAAAACGAGCGGTATTATAAGGGATTATGTTTCAGTTGCAAGCAAAAACTTTGCAATTTTGTTTAACTGTTTAAAATTCAACACATTCAATAATTTTGATCTATGTGAAATTTTTCCCTTACTTTCTTTTCTACTAGTGTAGTGGCTTTCCGGAAAAAATTCCCCTTCTTGTAAAATAAGAGTAGAATTATTTTAAATATTAAAAAAAAATTGAGTTTTCACTATG  >  1/544597‑545055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑