Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	539,602	C→T	P64P (CCC→CCT)	unknown →	Anaerobic respiratory reductase chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	539,602	0	C	T	100.0%	174.2 / NA	65	P64P (CCC→CCT)	unknown	Anaerobic respiratory reductase chaperone
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/31);  total (34/31)

CAAGAAAATCTGACCGCACTTTTGGCAAAGGATGGCTCCAAATAATGCAGGAAAGTGTGCAATTCAATAATTTTTCTTTGATTAGCCGTTTATTCGGTAATTTATTTTACCGCCAACCGACAGATCCCATTTTATCCGGCGTTTTTGCTTGGTTGCAGCAAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCCATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACGGCTTCCTGGTTGGAAGATAACACCGATTCATTGAGCGCCCAACAGGAATTATTTGAGCATTTTCTTCTGC  >  1/539367‑539846                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     caAGAAAATCTGACCGCACTTTTGGCAAAGGATGGCTCCAAATAATGCAGGAAAGTGTGCAATTCAATAATTTTTCTTTGATTAGCCGTTTATTCGGTAATTTATTTTACCGCCAACCGACAGATCCCATTTTATCCGGCGTTTTTGCTTGGTTGCAGCAAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACAttg                                                                                                                                                                                                                                       >  1:701026/1‑251 (MQ=255)       aaTCTGACCGCACTTTTGGCAAAGGATGGCTCCAAATAATGCAGGAAAGTGTGCAATTCAATAATTTTTCTTTGATTAGCCGTTTATTCGGTAATTTATTTTACCGCCAACCGACAGATCCCATTTTATCCGGCGTTTTTGCTTGGTTGCAGCAAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAAt                                                                                                                                                                                                                                 <  1:740087/251‑1 (MQ=255)                              ggATGGCTCCAAATAATGCAGGAAAGTGTGCAATTCAATAATTTTTCTTTGATTAGCCGTTTATTCGGTAATTTATTTTACCGCCAACCGACAGATCCCATTTTATCCGGCGTTTTTGCTTGGTTGCAGCAAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAAc                                                                                                                                                                                                                 >  1:70988/1‑244 (MQ=255)                                aTGGCTCCAAATAATGCAGGAAAGTGTGCAATTCAATAATTTTTCTTTGATTAGCCGTTTATTCGGTAATTTATTTTACCGCCAACCGACAGATCCCATTTTATCCGGCGTTTTTGCTTGGTTGCAGCAAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGt                                                                                                                                                                                                         <  1:545372/250‑1 (MQ=255)                                aTGGCTCCAAATAATGCAGGAAAGTGTGCAATTCAATAATTTTTCTTTGATTAGCCGTTTATTCGGTAATTTATTTTACCGCCAACCGACAGATCCCATTTTATCCGGCGTTTTTGCTTGGTTGCAGCAAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGt                                                                                                                                                                                                         <  1:480221/250‑1 (MQ=255)                                        aaaTAATGCAGGAAAGTGTGCAATTCAATAATTTTTCTTTGATTAGCCGTTTATTCGGTAATTTATTTTACCGCCAACCGACAGATCCCATTTTATCCGGCGTTTTTGCTTGGTTGCAGCAAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATc                                                                                                                                                                                                                                <  1:150920/219‑1 (MQ=255)                                        aaaTAATGCAGGAAAGTGTGCAATTCAATAATTTTTCTTTGATTAGCCGTTTATTCGGTAATTTATTTTACCGCCAACCGACAGATCCCATTTTATCCGGCGTTTTTGCTTGGTTGCAGCAAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGa                                                                                                                                                                                                >  1:782101/1‑251 (MQ=255)                                          ataatGCAGGAAAGTGTGCAATTCAATAATTTTTCTTTGATTAGCCGTTTATTCGGTAATTTATTTTACCGCCAACCGACAGATCCCATTTTATCCGGCGTTTTTGCTTGGTTGCAGCAAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAg                                                                                                                                                                                              <  1:824018/251‑1 (MQ=255)                                             atGCAGGAAAGTGTGCAATTCAATAATTTTTCTTTGATTAGCCGTTTATTCGGTAATTTATTTTACCGCCAACCGACAGATCCCATTTTATCCGGCGTTTTTGCTTGGTTGCAGCAAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGAt                                                                                                                                                                                                                                                <  1:196989/198‑1 (MQ=255)                                             atGCAGGAAAGTGTGCAATTCAATAATTTTTCTTTGATTAGCCGTTTATTCGGTAATTTATTTTACCGCCAACCGACAGATCCCATTTTATCCGGCGTTTTTGCTTGGTTGCAGCAAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAgtgt                                                                                                                                                                                           >  1:881851/1‑251 (MQ=255)                                                              aTTCAATAATTTTTCTTTGATTAGCCGTTTATTCGGTAATTTATTTTACCGCCAACCGACAGATCCCATTTTATCCGGCGTTTTTGCTTGGTTGCAGCAAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAg                                                                                                                                                                                              <  1:720765/231‑1 (MQ=255)                                                                                gATTAGCCGTTTATTCGGTAATTTATTTTACCGCCAACCGACAGATCCCATTTTATCCGGCGTTTTTGCTTGGTTGCAGCAAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTg                                                                                                                                                                                                                                                  >  1:796399/1‑161 (MQ=255)                                                                                gATTAGCCGTTTATTCGGTAATTTATTTTACCGCCAACCGACAGATCCCATTTTATCCGGCGTTTTTGCTTGGTTGCAGCAAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTg                                                                                                                                                        >  1:276340/1‑251 (MQ=255)                                                                                      ccGTTTATTCGGTAATTTATTTTACCGCCAACCGACAGATCACATTTTATCCGGCGTTTTTGCTTGGTTGCAGCAAGGTAATCTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGAtt                                                                                                                                                  >  1:410789/1‑251 (MQ=255)                                                                                             ttCGGTAATTTATTTTACCGCCAACCGACAGATCCCATTTTATCCGGCGTTTTTGCTTGGTTGCAGCAAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAggg                                                                                                                                           <  1:275816/251‑1 (MQ=255)                                                                                               cGGTAATTTATTTTACCGCCAACCGACAGATCCCATTTTATCCGGCGTTTTTGCTTGGTTGCAGCAAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGAtttt                                                                                                                                                >  1:687295/1‑244 (MQ=255)                                                                                                                   aaCCGACAGATCCCATTTTATCCGGCGTTTTTGCTTGGTTGCAGCAAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTgg                                                                                                                                                                                         <  1:148783/183‑1 (MQ=255)                                                                                                                   aaCCGACAGATCCCATTTTATCCGGCGTTTTTGCTTGGTTGCAGCAAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAAt                                                                                                                        >  1:84450/1‑248 (MQ=255)                                                                                                                        aCAGATCCCATTTTATCCGGCGTTTTTGCTTGGTTGCAGCAAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGa                                                                                                                >  1:838108/1‑251 (MQ=255)                                                                                                                           gATCCCATTTTATCCGGCGTTTTTGCTTGGTTGCAGCAAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGACAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGaaa                                                                                                              >  1:149754/1‑250 (MQ=255)                                                                                                                                cATTTTATCCGGCGTTTTTGCTTGGTTGCAGCAAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGaaa                                                                                                        >  1:645665/1‑251 (MQ=255)                                                                                                                                    ttATCCGGCGTTTTTGCTTGGTTGCAGCAAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAatat                                                                                                    <  1:111335/251‑1 (MQ=255)                                                                                                                                         cGGCGTTTTTGCTTGGTTGCAGCAAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATc                                                                                               <  1:812393/251‑1 (MQ=255)                                                                                                                                           gCGTTTTTGCTTGGTTGCAGCAAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAAc                                                                                                             >  1:592642/1‑235 (MQ=255)                                                                                                                                              tttttGCTTGGTTGCAGCAAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCAtttt                                                                                          >  1:144722/1‑251 (MQ=255)                                                                                                                                                            cagcaAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTa                                                                              >  1:569306/1‑249 (MQ=255)                                                                                                                                                               caAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGGATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGa                                                                          >  1:446510/1‑250 (MQ=255)                                                                                                                                                                aaGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACg                                                                        >  1:515431/1‑251 (MQ=255)                                                                                                                                                                 aGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAatat                                                                                                    >  1:147700/1‑222 (MQ=255)                                                                                                                                                                 aGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTa                                                                              >  1:18349/1‑244 (MQ=255)                                                                                                                                                                  ggTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAggg                                                                                                                                           >  1:821493/1‑182 (MQ=255)                                                                                                                                                                     aaTTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAggg                                                                                                                                           >  1:779191/1‑179 (MQ=255)                                                                                                                                                                     aaTTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAatat                                                                                                    <  1:585474/218‑1 (MQ=255)                                                                                                                                                                      aTTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACGGCTTcc                                                                  <  1:3785/251‑1 (MQ=255)                                                                                                                                                                          aTCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACGGCTTCCTGGt                                                              <  1:417806/251‑1 (MQ=255)                                                                                                                                                                           tCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACGGCTTCCTGGtt                                                             >  1:123843/1‑251 (MQ=255)                                                                                                                                                                           tCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACGGCTTCCTGGtt                                                             >  1:877849/1‑251 (MQ=255)                                                                                                                                                                            cccAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACGGCTTCCTGGTTg                                                            >  1:853348/1‑251 (MQ=255)                                                                                                                                                                                 gTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATg                                                                                    >  1:818287/1‑222 (MQ=255)                                                                                                                                                                                 gTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACGGCTTCCTGGTTGGAAg                                                        <  1:174793/250‑1 (MQ=255)                                                                                                                                                                                 gTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACGGCTTCCTGGTTGGAAGa                                                       >  1:46975/1‑251 (MQ=255)                                                                                                                                                                                      ggCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACGGCTTCCTGGTTGGAAGATAac                                                   <  1:247205/250‑1 (MQ=255)                                                                                                                                                                                      ggCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACGGCTTCCTGGTTGGAAGATAac                                                   <  1:178630/250‑1 (MQ=255)                                                                                                                                                                                       gCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACGGCTTCCTGGTTGGAAGATAaca                                                  >  1:822521/1‑250 (MQ=255)                                                                                                                                                                                        cGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACGGCTTCCTGGTTGGAAGATAacac                                                 <  1:301436/250‑1 (MQ=255)                                                                                                                                                                                        cGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACGGCTTCCTGGTTGGAAGATAacac                                                 <  1:384163/250‑1 (MQ=255)                                                                                                                                                                                        cGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACGGCTTCCTGGTTGGAAGATAacac                                                 <  1:145189/250‑1 (MQ=255)                                                                                                                                                                                        cGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACGGCTTCCTGGTTGGAAGATAACAcc                                                <  1:282387/251‑1 (MQ=255)                                                                                                                                                                                            aaaCGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACGGCTTCCTGGTTGGAAGATAACACCGAtt                                            >  1:56267/1‑251 (MQ=255)                                                                                                                                                                                             aaCGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACGGCTTCCTGGTTGGAAGATAACACCGATTc                                           <  1:555212/251‑1 (MQ=255)                                                                                                                                                                                                        cGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACGGCTTCCTGGTTGGAAGATAACACCGATTCATTGAgc                                    <  1:822044/247‑1 (MQ=255)                                                                                                                                                                                                           cGGTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACGGCTTCCTGGTTGGAAGATAACACCGATTCATTGAGCGCCCaa                              >  1:291015/1‑250 (MQ=255)                                                                                                                                                                                                            ggTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTg                                                                           <  1:59454/204‑1 (MQ=255)                                                                                                                                                                                                            ggTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACGGCTTCCTGGTTGGAAGATAACACCGATTCATTGAg                                     <  1:814577/242‑1 (MQ=255)                                                                                                                                                                                                             gTAAAAGCGCATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACGGCTTCCTGGTTGGAAGATAACACCGATTCATTGAGCGCCCAACAg                           >  1:331132/1‑251 (MQ=255)                                                                                                                                                                                                                      cATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCt                                                                               >  1:270805/1‑190 (MQ=255)                                                                                                                                                                                                                      cATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACGGCTTCCTGGTTGGAAGATAACACCGATTCATTg                                       <  1:34098/230‑1 (MQ=255)                                                                                                                                                                                                                      cATTAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACGGCTTCCTGGTTGGAAGATAACACCGATTCATTGAGCGCCCAACAGGAattatt                   <  1:853855/250‑1 (MQ=255)                                                                                                                                                                                                                        ttAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAggg                                                                                                                                           <  1:643208/128‑1 (MQ=255)                                                                                                                                                                                                                        ttAGATGCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACGGCTTCCTGGTTGGAAGATAACACCGATTCATTGAGCGCCCAACAGGAATTATTTGa                >  1:298237/1‑251 (MQ=255)                                                                                                                                                                                                                              gCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCa                                                                                      <  1:762677/175‑1 (MQ=255)                                                                                                                                                                                                                              gCCTTGCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACGGCTTCCTGGTTGGAAGATAACACCGATTCATTGAGCGCCCAACAGGAattatt                   >  1:446967/1‑242 (MQ=255)                                                                                                                                                                                                                                   gCAAAGACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAg                                                                                                                                                                                              <  1:682492/66‑1 (MQ=255)                                                                                                                                                                                                                                        gACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAAc                                                                                                                                                                                                                 >  1:462818/1‑42 (MQ=255)                                                                                                                                                                                                                                        gACCTATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACGGCTTCCTGGTTGGAAGATAACACCGATTCATTGAGCGCCCAACAGGAATTATTTGAGCATTTTCTTCTGc  <  1:652367/249‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     CAAGAAAATCTGACCGCACTTTTGGCAAAGGATGGCTCCAAATAATGCAGGAAAGTGTGCAATTCAATAATTTTTCTTTGATTAGCCGTTTATTCGGTAATTTATTTTACCGCCAACCGACAGATCCCATTTTATCCGGCGTTTTTGCTTGGTTGCAGCAAGGTAATTTATCCCAAGTCTGGGCGTTAAACGAAGACAGCGACGGTAAAAGCGCATTAGATGCCTTGCAAAGACCCATTGATTTGACATTGTTGAATCAAGAATATCAAAAACTTTTCGGTCAGTCGGGAAGTGTGGCGACACAAATTTCTGCTTATGAGATTTCCATTGAAGATTTTAAGGGTTTTCGGCAAATGCGCAATTTGCCTGAAACGGAAAATATCGATCATTTTGCCATGTTGCTACTGACGGCTTCCTGGTTGGAAGATAACACCGATTCATTGAGCGCCCAACAGGAATTATTTGAGCATTTTCTTCTGC  >  1/539367‑539846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑