Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	538,691	G→A	G116D (GGT→GAT)	unknown →	Putative activity regulator of membrane protease YbbK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	538,691	0	G	A	100.0%	149.2 / NA	55	G116D (GGT→GAT)	unknown	Putative activity regulator of membrane protease YbbK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/30);  total (25/30)

CGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCGCTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCCATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCAAAAACGGCATTGGTAGAGGCGCTTTCGGTGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATCCTAATCAACAAAGTTTTAACCGCGCCATCGTGGAACGTATTGTAAAAGCCTCTCATGAATTAGAGGCGGAAACCACTGTTCGGGATTTGTATACATTAAATTTCAAT  >  1/538455‑538934                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    cgccATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGga                                                                                                                                                                                                                                       <  1:276361/251‑1 (MQ=255)                       aTGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATTGCCACTTTTCTTTGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCCAAATCTTTCCTCAATCAACGGGGTCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATTGTACTACGTGGAGAATCCAGGGGCGTGATTTaa                                                                                                                                                                                                                  >  1:493556/1‑249 (MQ=255)                          aaaTTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAg                                                                                                                                                                                                               <  1:17632/250‑1 (MQ=255)                                 ttCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGat                                                                                                                                                                                                       <  1:879997/251‑1 (MQ=255)                                               cTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATAttgt                                                                                                                                                                                                  >  1:556646/1‑242 (MQ=255)                                                                          gTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAg                                                                                                                                                                                                               >  1:815593/1‑202 (MQ=255)                                                                                 ccATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGt                                                                                                                                                                                                                                          <  1:802633/168‑1 (MQ=255)                                                                                 ccATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGt                                                                                                                                                              >  1:607866/1‑244 (MQ=255)                                                                                        tCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAGTGATAttgttg                                                                                                                                                                                                <  1:632091/203‑1 (MQ=255)                                                                                           tAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGaa                                                                                                                                                                 <  1:239154/231‑1 (MQ=255)                                                                                                ttattTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGgaaagaaa                                                                                                                                        <  1:470473/251‑1 (MQ=255)                                                                                                  attTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATg                                                                                                                                      >  1:877390/1‑251 (MQ=255)                                                                                                     tggtggCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAg                                                                                                                                   <  1:495286/251‑1 (MQ=255)                                                                                                          gcgcTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAgtt                                                                                                                                                                                           <  1:235197/190‑1 (MQ=255)                                                                                                                      cGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTg                                                                                                                   >  1:677104/1‑250 (MQ=255)                                                                                                                      cGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTg                                                                                                                   >  1:513926/1‑250 (MQ=255)                                                                                                                        gTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAgggg                                                                                                                                                                                                                            >  1:135185/1‑143 (MQ=255)                                                                                                                        gTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAg                                                                                                                                                                                                               <  1:83754/156‑1 (MQ=255)                                                                                                                        gTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGatat                                                                                                                                                                                                     >  1:101934/1‑166 (MQ=255)                                                                                                                        gTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATAttgttg                                                                                                                                                                                                <  1:309675/171‑1 (MQ=255)                                                                                                                            ggATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATc                                                                                                             >  1:542854/1‑250 (MQ=255)                                                                                                                             taTCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCGCATc                                                                                                             <  1:159088/248‑1 (MQ=255)                                                                                                                              aTCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATCCt                                                                                                           <  1:237055/250‑1 (MQ=255)                                                                                                                                 aaaTTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATCCTAATc                                                                                                       <  1:151474/251‑1 (MQ=255)                                                                                                                                           aTCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGgag                                                                                                                                                                                                                                      >  1:10576/1‑114 (MQ=255)                                                                                                                                                         tcaatcaaCGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATG‑‑ATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATCCTAATCAACAAAGTTTTAACCGCGCCATCGTg                                                                             >  1:803750/1‑251 (MQ=255)                                                                                                                                                          caatcaaCGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAg                                                                                                                                                               >  1:689683/1‑170 (MQ=255)                                                                                                                                                              caaCGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAgggg                                                                                                                                                                                                                            <  1:814536/105‑1 (MQ=255)                                                                                                                                                              caaCGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATa                                                                                                                                                                              <  1:750664/151‑1 (MQ=255)                                                                                                                                                                   ggATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATCCTAATc                                                                                                       >  1:688846/1‑217 (MQ=255)                                                                                                                                                                   ggATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATCCTAATCAACAAAGTTTTAACCGCGCCATCGTGGGACGTAt                                                                     >  1:193095/1‑251 (MQ=255)                                                                                                                                                                    gATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAatta                                                                                                                         >  1:612764/1‑198 (MQ=255)                                                                                                                                                                         tgctaTGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGgaga                                                                                                                                                                                                                                     >  1:82362/5‑85 (MQ=255)                                                                                                                                                                         tgctaTGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAg                                                                                                                                                               <  1:570059/151‑1 (MQ=255)                                                                                                                                                                         tgctaTGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAg                                                                                                                                                               <  1:581536/151‑1 (MQ=255)                                                                                                                                                                         tgctaTGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATCCTAATCAACAAAGTTTTAACCGCGCCATCGTGGGACGTATTGTaaa                                                               <  1:337210/247‑1 (MQ=255)                                                                                                                                                                          gctaTGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACAt                                                                                                                                                                     <  1:560734/145‑1 (MQ=255)                                                                                                                                                                             aTGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATCCTAATCAACAAAGTTTTAACCGCGCCATCg                                                                               >  1:471786/1‑231 (MQ=255)                                                                                                                                                                              tGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGt                                                                                                                                                                                                                                          <  1:150573/75‑1 (MQ=255)                                                                                                                                                                               gATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATCCTAAt                                                                                                        >  1:360159/1‑204 (MQ=255)                                                                                                                                                                                     ggTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATc                                                                                                             <  1:235035/193‑1 (MQ=255)                                                                                                                                                                                      gTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAg                                                                                                                                                               <  1:604844/142‑1 (MQ=255)                                                                                                                                                                                           gggAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAatta                                                                                                                         >  1:355776/1‑175 (MQ=255)                                                                                                                                                                                                 aGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATCCTAATcaac                                                                                                    <  1:729774/190‑1 (MQ=255)                                                                                                                                                                                                  gTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATCCTAATCAACAAAGTTTTAACCGCGCCATCGTggga                                                                          <  1:464399/215‑3 (MQ=255)                                                                                                                                                                                                  gTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATCCTAATCAACAAAGTTTTAACCGCGCCATCGTGGGACGTATTGTAAAAGCCTCTCATGAATTAGAGGCGGaa                                      >  1:55444/1‑251 (MQ=255)                                                                                                                                                                                                            ttGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATCCTAATCAACAAAGTTTTAACCGCGCCATCGTGGGACGTATTGTAAAAGCCTCTCATGAATTAGAGGCGGAAACCACTGTTc                            <  1:781496/251‑1 (MQ=255)                                                                                                                                                                                                              gccgaaaaCGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATCCTAATca                                                                                                      <  1:408886/171‑1 (MQ=255)                                                                                                                                                                                                                        gCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAg                                                                                                                                   >  1:291831/1‑136 (MQ=255)                                                                                                                                                                                                                        gCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATCCTAATCAACAAAGTTTTAACCGCGCCATCGTGGGACGTATTGTAAAAGCCTCTCATGAATTAGAGGCGGAAACCACTGTTCGGGATTTGtat                 >  1:698408/1‑250 (MQ=255)                                                                                                                                                                                                                        gCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATCCTAATCAACAAAGTTTTAACCGCGCCATCGTGGGACGTATTGTAAAAGCCTCTCATGAATTAGAGGCGGAAACCACTGTTCGGGATTTGtat                 <  1:616620/250‑1 (MQ=255)                                                                                                                                                                                                                           ttGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATCCTAATCAACAAAGTTTTAACCGCGCCATCGTGGGACGTATTGTAAAAGCCTCTCATGAATTAGAGGCGGAAACCACTGTTCGGGATTTGTATACAt             >  1:44993/1‑251 (MQ=255)                                                                                                                                                                                                                            tGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGACAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGc                                                                                                                  <  1:677869/149‑1 (MQ=255)                                                                                                                                                                                                                                       cTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATc                                                                                                                                                                         <  1:689826/83‑1 (MQ=255)                                                                                                                                                                                                                                       cTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATCCTAATCAACAAAGTTTTAACCGCGCCATCGTGGGACGTATTGTAAAAGCCTCTCATGAATTAGAGGCGGAAACCACTGTTCGGGATTTGTATACATTAAATTTCAAt  >  1:765869/1‑250 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    CGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCGCTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCCATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCAAAAACGGCATTGGTAGAGGCGCTTTCGGTGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATCCTAATCAACAAAGTTTTAACCGCGCCATCGTGGAACGTATTGTAAAAGCCTCTCATGAATTAGAGGCGGAAACCACTGTTCGGGATTTGTATACATTAAATTTCAAT  >  1/538455‑538934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑