Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 538,626 C→T A94A (GCC→GCT)  unknown → Putative activity regulator of membrane protease YbbK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1538,6260CT100.0% 185.0 / NA 70A94A (GCC→GCT) unknownPutative activity regulator of membrane protease YbbK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/32);  total (38/32)

GGCTTATTCTTGGTTTTATCTTGCTCATTGCTGAAGTTGTCATTCCCGGTGTTTTTCTTCTATGGTGGGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCGCTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCCATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCAAAAACGGCATTGGTAGAGGCGCTTTCGGTGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATCCTAATCAACAAAGTTTTAACCGCGCCATCGTGGAACGTAT  >  1/538379‑538867
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 
ggCTTATTCTTGGTTTTATCTTGCTCATTGCTGAAGTTGTCATTCCCGGTGTTTTTCTTCTATGGTGGGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATg                                                                                                                                                                                                                                                >  1:862712/1‑251 (MQ=255)
ggCTTATTCTTGGTTTTATCTTGCTCATTGCTGAAGTTGTCATTCCCGGTGTTTTTCTTCTATGGTGGGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATg                                                                                                                                                                                                                                                >  1:513310/1‑251 (MQ=255)
ggCTTATTCTTGGTTTTATCTTGCTCATTGCTGAAGTTGTCATTCCCGGTGTTTTTCTTCTATGGTGGGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATg                                                                                                                                                                                                                                                >  1:42277/1‑251 (MQ=255)
ggCTTATTCTTGGTTTTATCTTGCTCATTGCTGAAGTTGTCATTCCCGGTGTTTTTCTTCTATGGTGGGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATg                                                                                                                                                                                                                                                >  1:17888/1‑251 (MQ=255)
  cTTATTCTTGGTTTTATCTTGCTCATTGCTGAAGTTGTCATTCCCGGTGTTTTTCTTCTATGGTGGGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGAt                                                                                                                                                                                                                                              <  1:858656/251‑1 (MQ=255)
  cTTATTCTTGGTTTTATCTTGCTCATTGCTGAAGTTGTCATTCCCGGTGTTTTTCTTCTATGGTGGGCATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGAt                                                                                                                                                                                                                                              <  1:643971/251‑1 (MQ=255)
     aTTCTTGGTTTTATCTTGCTCATTGCTGAAGTTGTCATTCCCGGTGTTTTTCTTCTATGGTGGGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAgg                                                                                                                                                                                                                                           >  1:740439/1‑251 (MQ=255)
     aTTCTTGGTTTTATCTTGCTCATTGCTGAAGTTGTCATTCCCGGTGTTTTTCTTCTATGGTGGGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGGTATCGCCATTATTCTTAGTATTATTTGGGGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAgg                                                                                                                                                                                                                                           >  1:733253/1‑251 (MQ=255)
       tCTTGGTTTTATCTTGCTCATTGCTGAAGTTGTCATTCCCGGTGTTTTTCTTCTATGGTGGGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAgggg                                                                                                                                                                                                                                         <  1:73398/251‑1 (MQ=255)
       tCTTGGTTTTATCTTGCTCATTGCTGAAGTTGTCATTCCCGGTGTTTTTCTTCTATGGTGGGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAgggg                                                                                                                                                                                                                                         <  1:792824/251‑1 (MQ=255)
          tGGTTTTATCTTGCTCATTGCTGAAGTTGTCATTCCCGGTGTTTTTCTTCTATGGTGGGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGc                                                                                                                                                                                                                                      <  1:471667/251‑1 (MQ=255)
                 aTCTTGCTCATTGCTGAAGTTGTCATTCCCGGTGTTTTTCTTCTATGGTGGGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTTTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGGTCATGCTATGAt                                                                                                                                                                                                                                              >  1:378852/1‑236 (MQ=255)
                 aTCTTGCTCATTGCTGAAGTTGTCATTCCCGGTGTTTTTCTTCTATGGTGGGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTCTCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGGGCAGGGaaa                                                                                                                                                                                                                               >  1:443785/1‑251 (MQ=255)
                           ttGCTGAAGTTGTCATTCCCGGTTTTTTTCTTCTATGGTGGGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCGTTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGa                                                                                                                                                                                                                                 <  1:374284/239‑1 (MQ=255)
                           ttGCTGAAGTTGTCATTCCCGGTGTTTTTCTTCTATGGTGGGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTaa                                                                                                                                                                                                                       >  1:57603/1‑249 (MQ=255)
                           ttGCTGAAGTTGTCATTCCCGGTGTTTTTCTTCTATGGTGGGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACt                                                                                                                                                                                                                     <  1:225832/251‑1 (MQ=255)
                           ttGCTGAAGTTGTCATTCCCGGTGTTTTTCTTCTATGGTGGGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGGGCAGGGAAAAGTCTTaa                                                                                                                                                                                                                       >  1:263504/1‑249 (MQ=255)
                           ttGCTGAAGTTGTCATTCCCGGTGTTTTTCTTCTATGGTGGGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGGGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTaa                                                                                                                                                                                                                       >  1:672340/1‑249 (MQ=255)
                            tGCTGAAGTTGTCATTCCCGGTGTTTTTCTTCTATGGTGGGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACt                                                                                                                                                                                                                     >  1:498080/1‑250 (MQ=255)
                              cTGAAGTTGTCATTCCCGGTGTTTTTCTTCTATGGTGGGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTAt                                                                                                                                                                                                                   <  1:187093/250‑1 (MQ=255)
                                gAAGTTGTCATTCCTGGTGTTTTTCTTCTATGGTGGGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTg                                                                                                                                                                                                                 >  1:215017/1‑250 (MQ=255)
                                  aGTTGTCATTCCCGGTGTTTTTCTTCTATGGTGGGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGAt                                                                                                                                                                                                                                              <  1:497247/219‑1 (MQ=255)
                                                gtgtTTTTCTTCTATGGTGGGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGt                                                                                                                                                                                                >  1:526834/1‑251 (MQ=255)
                                                gtgtTTTTCTTCTATGGTGGGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAAATTCTTCGGTTATCGCCCTTATTCTTAGTATTTTTTGGGGGCGCTATCAACACGGTTAGGATCAAATTGATCAATCGAAATCTGTCCTTAATCAACGGGATTATGCTATGATAGGGGTGCAGGGAAAAGTCTTTACTATTGCCGAAAACGGCATTggg                                                                                                                                                                                                >  1:597405/1‑250 (MQ=255)
                                                  gtTTTTCTTCTATGGTGGGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATa                                                                                                                                                                                                                                             <  1:313049/204‑1 (MQ=255)
                                                           ctATGGTGGGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTTATTTATGCGGTTATCGCCATTATTCTTTGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACCGCATTGGTAGAGGCGCttt                                                                                                                                                                                     >  1:173140/1‑251 (MQ=255)
                                                                 tggGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCgatg                                                                                                                                                                                <  1:17564/250‑4 (MQ=255)
                                                                     gATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTTTTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAg                                                                                                                                                                                                                                            >  1:802096/1‑186 (MQ=255)
                                                                            cgccATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGga                                                                                                                                                                    <  1:276361/251‑1 (MQ=255)
                                                                                                  aTGAAATTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATTGCCACTTTTCTTTGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCCAAATCTTTCCTCAATCAACGGGGTCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATTGTACTACGTGGAGAATCCAGGGGCGTGATTTaa                                                                                                                                               >  1:493556/1‑249 (MQ=255)
                                                                                                     aaaTTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAg                                                                                                                                            <  1:17632/250‑1 (MQ=255)
                                                                                                      aaTTTATTCACGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCCTTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGgcg                                                                                                                                                                                         >  1:235606/1‑204 (MQ=255)
                                                                                                       aTTTATTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTTTTTGGTGGCGCTATCAAAACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTa                                                                                                                                                                                               >  1:179321/1‑197 (MQ=255)
                                                                                                           aTTCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGGTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGGCTTAACTATTGCCGAAAACGGCATTGGt                                                                                                                                                                                                >  1:855593/1‑192 (MQ=255)
                                                                                                            ttCCCGCCTTACCACTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGat                                                                                                                                    <  1:879997/251‑1 (MQ=255)
                                                                                                                          cTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATAttgt                                                                                                                               >  1:556646/1‑242 (MQ=255)
                                                                                                                           tttCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGAt                                                                                                                                                                                                                                              <  1:481054/130‑1 (MQ=255)
                                                                                                                                           aaTTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACt                                                                                                                                                                                                                     <  1:846044/139‑1 (MQ=255)
                                                                                                                                             tttATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTa                                                                                                                                                                                                                    <  1:182531/138‑1 (MQ=255)
                                                                                                                                                     gTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAg                                                                                                                                            >  1:815593/1‑202 (MQ=255)
                                                                                                                                                            ccATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGt                                                                                                                                                                       <  1:802633/168‑1 (MQ=255)
                                                                                                                                                            ccATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGt                                                                                           >  1:607866/1‑244 (MQ=255)
                                                                                                                                                                 attCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAgggg                                                                                                                                                                                                                                         <  1:66803/97‑1 (MQ=255)
                                                                                                                                                                   tCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAGTGATAttgttg                                                                                                                             <  1:632091/203‑1 (MQ=255)
                                                                                                                                                                      tAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGaa                                                                                              <  1:239154/231‑1 (MQ=255)
                                                                                                                                                                           ttattTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGgaaagaaa                                                                     <  1:470473/251‑1 (MQ=255)
                                                                                                                                                                             attTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATg                                                                   >  1:877390/1‑251 (MQ=255)
                                                                                                                                                                                tggtggCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAg                                                                <  1:495286/251‑1 (MQ=255)
                                                                                                                                                                                     gcgcTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAgtt                                                                                                                        <  1:235197/190‑1 (MQ=255)
                                                                                                                                                                                          aTCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGAt                                                                                                                                                                                                                                              <  1:656336/67‑1 (MQ=255)
                                                                                                                                                                                                 cGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTg                                                >  1:513926/1‑250 (MQ=255)
                                                                                                                                                                                                 cGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTg                                                >  1:677104/1‑250 (MQ=255)
                                                                                                                                                                                                   gTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTa                                                                                                                                                                                                                    >  1:538890/1‑84 (MQ=255)
                                                                                                                                                                                                   gTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAgggg                                                                                                                                                         >  1:135185/1‑143 (MQ=255)
                                                                                                                                                                                                   gTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAg                                                                                                                                            <  1:83754/156‑1 (MQ=255)
                                                                                                                                                                                                   gTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGatat                                                                                                                                  >  1:101934/1‑166 (MQ=255)
                                                                                                                                                                                                   gTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATAttgttg                                                                                                                             <  1:309675/171‑1 (MQ=255)
                                                                                                                                                                                                       ggATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATc                                          >  1:542854/1‑250 (MQ=255)
                                                                                                                                                                                                        taTCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCGCATc                                          <  1:159088/248‑1 (MQ=255)
                                                                                                                                                                                                         aTCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATCCt                                        <  1:237055/250‑1 (MQ=255)
                                                                                                                                                                                                            aaaTTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGt                                                                                                                                                                                                                            >  1:676669/1‑67 (MQ=255)
                                                                                                                                                                                                            aaaTTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATCCTAATc                                    <  1:151474/251‑1 (MQ=255)
                                                                                                                                                                                                                      aTCGAAATCTGTCCTCAATCAACGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGgag                                                                                                                                                                   >  1:10576/1‑114 (MQ=255)
                                                                                                                                                                                                                                    tcaatcaaCGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATG‑‑ATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATCCTAATCAACAAAGTTTTAACCGCGCCATCGTg          >  1:803750/1‑251 (MQ=255)
                                                                                                                                                                                                                                     caatcaaCGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAg                                                                                            >  1:689683/1‑170 (MQ=255)
                                                                                                                                                                                                                                         caaCGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAgggg                                                                                                                                                         <  1:814536/105‑1 (MQ=255)
                                                                                                                                                                                                                                         caaCGGGATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATa                                                                                                           <  1:750664/151‑1 (MQ=255)
                                                                                                                                                                                                                                              ggATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATCCTAATc                                    >  1:688846/1‑217 (MQ=255)
                                                                                                                                                                                                                                              ggATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATCCTAATCAACAAAGTTTTAACCGCGCCATCGTGGGACGTAt  >  1:193095/1‑251 (MQ=255)
                                                                                                                                                                                                                                               gATCATGCTATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCGAAAACGGCATTGGTAGAGGCGCTTTCGATGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAatta                                                      >  1:612764/1‑198 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 
GGCTTATTCTTGGTTTTATCTTGCTCATTGCTGAAGTTGTCATTCCCGGTGTTTTTCTTCTATGGTGGGGATTAGCCGCCATCGTTGTTGCCGGTATCATGAAATTTATTCCCGCCTTACCGCTTTCGGCATTGGCGGTAATTTATGCGGTTATCGCCATTATTCTTAGTATTATTTGGTGGCGCTATCAACACGGTAAGGATCAAATTGATCAATCGAAATCTGTCCTCAATCAACGGGATCATGCCATGATAGGGGTGCAGGGAAAAGTCTTAACTATTGCCAAAAACGGCATTGGTAGAGGCGCTTTCGGTGATACTACGTGGAGAATCCAGGGGCGTGATTTAGCAGTGAATGATATTGTTGAAGTTGTTTCTGTTGATAGCATCACATTGAAAGTTAAGAAAATTTAAGGAAAGAAATGAAGCATTTAATTATTTCTGCACATCCTAATCAACAAAGTTTTAACCGCGCCATCGTGGAACGTAT  >  1/538379‑538867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑