Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	535,347	G→A	intergenic (+68/+5)	unknown → / ← unknown	F200/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	535,347	0	G	A	100.0%	42.9 / NA	18	intergenic (+68/+5)	unknown/unknown	F200/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/9);  total (9/9)

GTTTTATTATTAAAGATATTATACTTATTGGCTCCGGTCTTGTGTTGTTAAAACACGACCTAAATAAAATTCCATTTGTTAAATAGTCGGTAATAAATAAGTTCGCCTCAATTTAGGCGAACTTATTTTTGTATTTATAAAGTACTATAATAAGTAACTTATTCAGTAACACTTTCAGTTATTAACTTCACCAGTTTATTCATTTTTTCTACATTCCCTGCCGTGACATTATGTTTTTCTGCTATAATCTCATTTCTATTTAGACTTACGAATATTTCACCTTGGCTATTCTCCCATACTAAAGCTTTTAATGGTAAATCAATAGCTAGGGTTGGGGATTCAATCATCATTGGGGTACCTAGTTGCGGTTTTCCAAAAACAATTACACTTGAAAACGGC  >  1/535194‑535592                                                                                                                                                          |                                                                                                                                                                                                                                                      gTTTTATTATTAAAGATATTATACTTATTGGCTCCGGTCTTGTGTTGTTAAAACACGACCTAAATAAAATTCCATTTGTTAAATAGTCGGTAATAAATAAGTTCGCCTCAATTTAGGCGAACTTATTTTTGTATTTATAAAGTACTATAATAAATAACTTATTCAGTAACACTTTCAGTTATTAACTTCACCAGTTTATTCATTTTTTCTACATTCCCTGCCGTGACATTATGTTTTTCTGCTATAAtct                                                                                                                                                       >  1:29432/1‑250 (MQ=255)                atatTATACTTATTGGCTCCGGTCTTGTGTTGTTAAAACACGACCTAAATAAAATTCCATTTGTTAAATAGTCGGTAATAAATAAGTTCGCCTCAATTTAGGCGAACTTATTTTTGTATTTATAAAGTACTATAATAAATAACTTATTCAGTAACACTTTCAGTTATTAACTTCACCAGTTTATTCATTTTTTCTACATTCCCTGCCGTGACATTATGTTTTTCTGCTATAATCTCATTTc                                                                                                                                                 <  1:706042/241‑1 (MQ=255)                                cTCCGGTCTTGTGTTGTTAAAACACGACCTAAATAAAATTCCATTTGTTAAATAGTCGGTAATAAATAAGTTCGCCTCAATTTAGGCGAACTTATTTTTGTATTTATAAAGTACTATAATAAATAACTTATTCAGTAACACTTTCAGTTATTAACTTCACCAGTTTATTCATTTTTTCTACATTCCCTGCCGTGACATTATGTTTTTCTGCTATAATCTCATTTCTATTTAGACTTACGAATATTTCAcc                                                                                                                        <  1:879326/250‑1 (MQ=255)                                               gttAAAACACGACCTAAATAAAATTCCATTTGTTAAATAGTCGGTAATAAATAAGTTCGCCTCAATTTAGGCGAACTTATTTTTGTATTTATAAAGTACTATAATAAATAACTTATTCAGTAACACTTTCAGTTATTAACTTCACCAGTTTATTCATTTTTTCTACATTCCCTGCCGTGACATTATGTTTTTCTGCTATAATCTCATTTCTATTTAGACTTACGAATATTTCACCTTGGCTATTCTCCCAt                                                                                                        >  1:171345/1‑251 (MQ=255)                                                       acGACCTAAATAAAATTCCATTTGTTAAATAGTCGGTAATAAATAAGTTCGCCTCAATTTAGGCGAACTTATTTTTGTATTTATAAAGTACTATAATAAATAACTTATTCAGTAACACTTTCAGTTATTAACTTCACCAGTTTATTCATTTTTTCTACATTCCCTGCCGTGACATTATGTTTTTCTGCTATAATCTCATTTCTATTTAGACTTACGAATATTTCACCTTGGCTATTCTCCCATACTAAAGc                                                                                                <  1:770850/251‑1 (MQ=255)                                                               aataaaATTCCATTTGTTAAATAGTCGGTAATAAATAAGTTCGCCTCAATTTAGGCGAACTTATTTTTGTATTTATAAAGTACTATAATAAATAACTTATTCAGTAACACTTTCAGTTATTAACTTCACCAGTTTATTCATTTTTTCTACATTCCCTGCCGTGACATTATGTTTTTCTGCTATAATCTCATTTCTATTTAGACTTACGAATATTTCACCTTGGCTATTCTCCCATAc                                                                                                      >  1:93795/1‑237 (MQ=255)                                                                ataaaATTCCATTTGTTAAATAGTCGGTAATAAATAAGTTCGCCTCAATTTAGGCGAACTTATTTTTGTATTTATAAAGTACTATAATAAATAACTTATTCAGTAACACTTTCAGTTATTAACTTCACCAGTTTATTCATTTTTTCTACATTCCCTGCCGTGACATTATGTTTTTCTGCTATAATCTCATTTCTATTTAGACTTACGAATATTTCACCTTGGCTATTCTCCCATACTAAAGc                                                                                                >  1:302115/1‑242 (MQ=255)                                                                                  aaTAGTCGGTAATAAATAAGTTCGCCTCAATTTAGGCGAACTTATTTTTGTATTTATAAAGTACTATAATAAATAACTTATTCAGTAACACTTTCAGTTATTAACTTCACCAGTTTATTCATTTTTTCTACATTCCCTGCCGTGACATTATGTTTTTCTGCTATAATCTCATTTCTATTTAGACTTACGAATATTTCACCTTGGCTATTCTCCCATACTAAAGCTTTTAATGGTAAATCAATAGCTAGGGt                                                                     <  1:703797/251‑1 (MQ=255)                                                                                      gTCGGTAATAAATAAGTTCGCCTCAATTTAGGCGAACTTATTTTTGTATTTATAAAGTACTATAATAAATAACTTATTCAGTAACACTTTCAGTTATTAACTTCACCAGTTTATTCATTTTTTCTACATTCCCTGCCGTGACATTATGTTTTTCTGCTATAATCTCATTTCTATTTAGACTTACGAATATTTCACCTTGGCTATTCTCCCATACTAAAGCTTTTAATGGTAAATCAAAAGCTAGGGTTggg                                                                 >  1:553428/1‑251 (MQ=255)                                                                                               aaataaGTTCGCCTCAATTTAGGCGAACTTATTTTTGGATTTATAAAGTACTATAATAAATAACTTATTCAGTAACACTTTCAGTTATTAACTTCACCAGTTTATTCATTTTTTCTACATTCCCTGCCGTGACATTATGTTTTTCTGCTATAATCTCATTTCTATTTAGACTTACGAATATTTCACCTTGGCTATTCTCCCATACTa                                                                                                    <  1:437461/207‑1 (MQ=255)                                                                                                                                   gTATTTATAAAGTACTATAATAAATAACTTATTCAGTAACACTTTCAGTTATTAACTTCACCAGTTTATTCATTTTTTCTACATTCCCTGCCGTGACATTATGTTTTTCTGCTATAATCTCATTTCTATTTAGACTTACGAATATTTCACCTTGGCTATTCTCCCa                                                                                                         >  1:602493/1‑166 (MQ=255)                                                                                                                                       ttATAAAGTACTATAATAAATAACTTATTCAGTAACACTTTCAGTTATTAACTTCACCAGTTTATTCATTTTTTCTACATTCCCTGCCGTGACATTATGTTTTTCTGCTATAATCTCATTTCTATTTAGACTTACGAATATTTCACCTTGGCTATTCTCCCATACTAAAGCTTTTAAt                                                                                         >  1:680840/1‑178 (MQ=255)                                                                                                                                              gTACTATAATAAATAACTTATTCAGTAACACTTTCAGTTATTAACTTCACCAGTTTATTCATTTTTTCTACATTCCCTGCCGTGACATTATGTTTTTCTGCTATAATCTCATTTCTATTTAGACTTACGAATATTTCACCTTGGCTATTCTCCCATACTAAAGCTTTTAATGGTAAATCAATAGCTAGGGTTGGGGATTCAATCATCATTgggg                                              <  1:411021/214‑1 (MQ=255)                                                                                                                                              gTACTATAATAAATAACTTATTCAGTAACACTTTCAGTTATTAACTTCACCAGTTTATTCATTTTTTCTACATTCCCTGCCGTGACATTATGTTTTTCTGCTATAATCTCATTTCTATTTAGACTTACGAATATTTCACCTTGGCTATTCTCCCATACTAAAGCTTTTAATGGTAAATCAATAGCTAGGGTTGGGGATTCAATCATCATTGGGGTACCTAGTTGCGGTTTTCCAAAAACAATTACACTTGa         >  1:901766/1‑251 (MQ=255)                                                                                                                                                   ataataAATAACTTATTCAGTAACACTTTCAGTTATTAACTTCACCAGTTTATTCATTTTTTCTACATTCCCTGCCGTGACATTATGTTTTTCTGCTATAATCTCATTTCTATTTAGACTTACGaa                                                                                                                                 >  1:720419/1‑126 (MQ=255)                                                                                                                                                   ataataAATAACTTATTCAGTAACACTTTCAGTTATTAACTTCACCAGTTTATTCATTTTTTCTACATTCCCTGCCGTGACATTATGTTTTTCTGCTATAATCTCATTTCTATTTAGACTTACGAATATTTCACCTTGGCTATTCTCCCATACTAAAGCTTTTAATGGTAAATCAATAGCTAGGGTTGGGGATTCAATCATCATTgggg                                              <  1:126476/209‑1 (MQ=255)                                                                                                                                                   ataataAATAACTTATTCAGTAACACTTTCAGTTATTAACTTCACCAGTTTATTCATTTTTTCTACATTCCCTGCCGTGACATTATGTTTTTCTGCTATAATCTCATTTCTATTTAGACTTACGAATATTTCACCTTGGCTATTCTCCCATACTAAAGCTTTTAATGGTAAATCAATAGCTAGGGTTGGGGATTCAATCATCATTGGGGTACCTAGTTGCGGtt                               <  1:294511/224‑1 (MQ=255)                                                                                                                                                   ataataAATAACTTATTCAGTAACACTTTCAGTTATTAACTTCACCAGTTTATTCATTTTTTCTACATTCCCTGCCGTGACATTATGTTTTTCTGCTATAATCTCATTTCTATTTAGACTTACGAATATTTCACCTTGGCTATTCTCCCATACTAAAGCTTTTAATGGTAAATCAATAGCTAGGGTTGGGGATTCAATCATCATTGGGGTACCTAGTTGCGGTTTTCCAAAAACAATTACACTTGAAAAc     <  1:781019/250‑1 (MQ=255)                                                                                                                                                     aataaataACTTATTCAGTAACACTTTCAGTTATTAACTTCACCAGTTTATTCATTTTTTCTACATTCCCTGCCGTGACATTATGTTTTTCTGCTATAATCTCATTTCTATTTAGACTTACGAATATTTCACCTTGGCTATTCTCCCATACTAAAGCTTTTAATGGTAAATCAATAGCTAGGGTTGGGGATTCAATCATCATTGGGGTACCTAGTTGCGGTTTTCCAAAAACAATTACACTTGAAAACgg   >  1:543366/1‑250 (MQ=255)                                                                                                                                                     aataaataACTTATTCAGTAACACTTTCAGTTATTAACTTCACCAGTTTATTCATTTTTTCTACATTCCCTGCCGTGACATTATGTTTTTCTGCTATAATCTCATTTCTATTTAGACTTACGAATATTTCACCTTGGCTATTCTCCCATACTAAAGCTTTTAATGGTAAATCAATAGCTAGGGTTGGGGATTCAATCATCATTGGGGTACCTAGTTGCGGTTTTCCAAAAACAATTACACTTGAAAACGGc  <  1:398090/251‑1 (MQ=255)                                                                                                                                                          |                                                                                                                                                                                                                                                      GTTTTATTATTAAAGATATTATACTTATTGGCTCCGGTCTTGTGTTGTTAAAACACGACCTAAATAAAATTCCATTTGTTAAATAGTCGGTAATAAATAAGTTCGCCTCAATTTAGGCGAACTTATTTTTGTATTTATAAAGTACTATAATAAGTAACTTATTCAGTAACACTTTCAGTTATTAACTTCACCAGTTTATTCATTTTTTCTACATTCCCTGCCGTGACATTATGTTTTTCTGCTATAATCTCATTTCTATTTAGACTTACGAATATTTCACCTTGGCTATTCTCCCATACTAAAGCTTTTAATGGTAAATCAATAGCTAGGGTTGGGGATTCAATCATCATTGGGGTACCTAGTTGCGGTTTTCCAAAAACAATTACACTTGAAAACGGC  >  1/535194‑535592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑