Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	529,589	A→T	Q109L (CAG→CTG)	unknown →	Starvation lipoprotein Slp paralog

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	529,589	0	A	T	100.0%	165.3 / NA	62	Q109L (CAG→CTG)	unknown	Starvation lipoprotein Slp paralog
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (25/37);  total (25/37)

AAACAGTTCGAAATGTTAAATCTGAAAAATGTGCAGGCGCAGGATTACGATTGCCGCTGCCTGAAAGTGCGCTTGGGCGGCAAAGTGGTTTCCGCACAGGCGTTGAAAGATAAAACCAAAATTGAAGTATTGAATCAAACGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCAGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTATGACTACGCACCGTTTTACCCTTACGGCTGGGGATTTTCGTCCCGCTTCTTTTGGGCTGAACCGCGCGTGCGCTATAATTTATATTAATTAACC  >  1/529348‑529827                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               aaaCAGTTCGAAATGTTAAATCTGAAAAATGTGCAGGCGCAGGATTACGATTGCCGCTGCCTGAAAGTGCGCTTGGGCGGCAAAGTGGTTTCCGCACAGGCGTTGAAAGATAAAACCAAAATTGAAGTATTGAATCAAACGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGAc                                                                                                                                                                                                                                       <  1:709117/251‑1 (MQ=255)                         aaaaaTGTGCAGGCGCAGGATTACGATTGCCGCTGCCTGAAAGTGCGCTTGGGCGGCAAAGTGGTTTCCGCACAGGCGTTGAAAGATAAAACCAAAATTGAAGTATTGAATCAAACGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCaa                                                                                                                                                                                                               <  1:159840/251‑1 (MQ=255)                              tgtgCAGGCGCAGGATTACGATTGCCGCTGCCTGAAAGTGCGCTTGGGCGGCAAAGTGGTTTCCGCACAGGCGTTGAAAGATAAAACCAAAATTGAAGTATTGAATCAAACGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGc                                                                                                                                                                                                          <  1:180773/251‑1 (MQ=255)                               gtgCAGGCGCAGGATTACGATTGCCGCTGCCTGAAAGTGCGCTTGGGCGGCAATGTGGTTTCCGCACAGGCGTTGAAAGATAAAACCAAAATTGAAGTATTGAATCAAACGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAg                                                                                                                                                                                                                                     <  1:557586/223‑1 (MQ=255)                                                 gATTGCCGCTGCCTGAAAGTGCGCTTGGGCGGCAAAGTGGTTTCCGCACAGGCGTTGAAAGATAAAACCAAAATTGAAGTATTGAATCAAACGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGc                                                                                                                                                                                                     <  1:471378/237‑1 (MQ=255)                                                          tGCCTGAAAGTGCGCTTGGGCGGCAAAGTGGTTTCCGCACAGGCGTTGAAAGATAAAACCAAAATTGAAGTATTGAATCAAACGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACaa                                                                                                                                                                              <  1:151047/251‑1 (MQ=255)                                                          tGCCTGAAAGTGCGCTTGGGCGGCAAAGTGGTTTCCGCACAGGCGTTGAAAGATAAAACCAAAATTGAAGTATTGAATCAAACGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTACTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACaa                                                                                                                                                                              <  1:356716/251‑1 (MQ=255)                                                            ccTGAAAGTGCGCTTGGGCGGCAAAGTGGTTTCCGCACAGGCGTTGAAAGATAAAACCAAAATTGAAGTATTGAATCAAACGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAAt                                                                                                                                                                             <  1:414764/250‑1 (MQ=255)                                                            ccTGAAAGTGCGCTTGGGCGGCAAAGTGGTTTCCGCACAGGCGTTGAAAGATAAAACCAAAATTGAAGTATTGAATCAAACGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAAt                                                                                                                                                                             <  1:598867/250‑1 (MQ=255)                                                                aaaGTGCGCTTGGGCGGCAAAGTGGTTTCCGCACAGGCGTTGAAAGATAAAACCAAAATTGAAGTATTGAATCAAACGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATcc                                                                                                                                                                        <  1:357546/251‑1 (MQ=255)                                                                aaaGTGCGCTTGGGCGGCAAAGTGGTTTCCGCACAGGCGTTGAAAGATAAAACCAAAATTGAAGTATTGAATCAAACGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATcc                                                                                                                                                                        <  1:845272/251‑1 (MQ=255)                                                                             gcggcAAAGTGGTTTCCGCACAGGCGTTGAAAGATAAAACCAAAATTGAAGTATTGAATCAAACGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGc                                                                                                                                                            <  1:531/250‑1 (MQ=255)                                                                              cggcAAAGTGGTTTCCGCACAGGCGTTGAAAGATAAAACCAAAATTGAAGTATTGAATCAAACGGTGGCGTATTTTTCCGTGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGcc                                                                                                                                                           <  1:425766/250‑1 (MQ=255)                                                                              cggcAAAGTGGTTTCCGCACAGGCGTTGAAAGATAAAACCAAAATTGAAGTATTGAATCAAACGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGcc                                                                                                                                                           <  1:80859/250‑1 (MQ=255)                                                                                      tGGTTTCCGCACAGGCGTTGAAAGATAAAACCAAAATTGAAGTATTGAATCAAACGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATAc                                                                                                                                                  <  1:533115/251‑1 (MQ=255)                                                                                      tGGTTTCCGCACAGGCGTTGAAAGATAAAACCAAAATTGAAGTATTGAATCAAACGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATAc                                                                                                                                                  <  1:717899/251‑1 (MQ=255)                                                                                       ggTTTCCGCACAGGCGTTGAAAGATAAAACCAAAATTGAAGTATTGAATCAAACGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATAc                                                                                                                                                  >  1:708244/1‑250 (MQ=255)                                                                                           tCCGCACAGGCGTTGAAAGATAAAACCAAAATTGAAGTATTGAATCAAACGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTAt                                                                                                                                             <  1:365300/251‑1 (MQ=255)                                                                                                    gCGTTGAAAGATAAAACCAAAATTGAAGTATTGAATCAAACGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGc                                                                                                                                     <  1:610601/250‑1 (MQ=255)                                                                                                                    ccAAAATTGAAGTATTGAATCAAACGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAAtattat                                                                                                                    <  1:594449/251‑1 (MQ=255)                                                                                                                            gAAGTATTGAATCAAACGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGAcc                                                                                                             >  1:2291/1‑250 (MQ=255)                                                                                                                               gTATTGAATCAAACGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATAc                                                                                                                                                  >  1:586953/1‑210 (MQ=255)                                                                                                                                    gAATCAAACGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGCCGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACa                                                                                                    >  1:870312/1‑251 (MQ=255)                                                                                                                                      aTCAAACGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGc                                                                                                                                                            <  1:290325/193‑1 (MQ=255)                                                                                                                                         aaaCGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTAt                                                                                               >  1:775349/1‑251 (MQ=255)                                                                                                                                         aaaCGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTAt                                                                                               >  1:272145/1‑251 (MQ=255)                                                                                                                                         aaaCGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTAt                                                                                               >  1:688771/1‑251 (MQ=255)                                                                                                                                            cGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTATGAc                                                                                            <  1:651100/251‑1 (MQ=255)                                                                                                                                                   gTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATAttatta                                                                                                                  <  1:516734/222‑1 (MQ=255)                                                                                                                                                   gTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTATGACTACGCa                                                                                      >  1:31341/1‑250 (MQ=255)                                                                                                                                                                  aCCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATcc                                                                                                                                                                        <  1:457561/153‑1 (MQ=255)                                                                                                                                                                      aTCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTATGACTACGCACCGTTTTAccc                                                                           <  1:61572/242‑1 (MQ=255)                                                                                                                                                                         gTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTATGAc                                                                                            <  1:368704/222‑1 (MQ=255)                                                                                                                                                                               aGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTATGACTACGCACCGTTTTACCCTTACGGCTGGGGAtttt                                                          >  1:419038/1‑250 (MQ=255)                                                                                                                                                                                gCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTg                                                                                                                                                                                                                       >  1:263042/1‑92 (MQ=255)                                                                                                                                                                                gCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGc                                                                                                                                                                                                     >  1:726408/1‑110 (MQ=255)                                                                                                                                                                                gCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATc                                                                                                                                                       <  1:281803/156‑1 (MQ=255)                                                                                                                                                                                gCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATAc                                                                                                                                                  >  1:240960/1‑161 (MQ=255)                                                                                                                                                                                gCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTATGACTACGCACCGTTTTAcc                                                                            <  1:239530/231‑1 (MQ=255)                                                                                                                                                                                gCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTATGACTACGCACCGTTTTACCCTTACGGCTGGGGATTTTCg                                                        >  1:549499/1‑251 (MQ=255)                                                                                                                                                                                gCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTATGACTACGCACCGTTTTACCCTTACGGCTGGGGATTTTCg                                                        >  1:817153/1‑251 (MQ=255)                                                                                                                                                                                gCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTATGACTACGCACCGTTTTACCCTTACGGCTGGGGATTTTCg                                                        >  1:745145/1‑251 (MQ=255)                                                                                                                                                                                gCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTATGACTACGCACCGTTTTACCCTTACGGCTGGGGATTTTCg                                                        >  1:755557/1‑251 (MQ=255)                                                                                                                                                                                gCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTATGACTACGCACCGTTTTACCCTCACGGCTgggg                                                               >  1:878040/1‑244 (MQ=255)                                                                                                                                                                                 cTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTATGAc                                                                                            >  1:800376/1‑214 (MQ=255)                                                                                                                                                                                 cTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTATGACTACGCACCGTTTTACCCTTACGGCTGGGGATTTTCGt                                                       >  1:765091/1‑251 (MQ=255)                                                                                                                                                                                   ataGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTATg                                                                                              >  1:723027/1‑210 (MQ=255)                                                                                                                                                                                       cAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTATGACTACGCACCGTTTTACCCTTACGGCTGGGGATTTTCGTCCCg                                                   >  1:457506/1‑249 (MQ=255)                                                                                                                                                                                                 tttATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTATGACTACGCACCGTTTTACCCTTACGGCTGGGGATTTTCGTCCCGCTTCTTTTggg                                        >  1:15489/1‑250 (MQ=255)                                                                                                                                                                                                  ttATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGAc                                                                                                                                                                                                                                       <  1:650411/58‑1 (MQ=255)                                                                                                                                                                                                      cGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTATGACTACGCACCGTTTTACCCTTACGGCTGGGGATTTTCGTCCCGCTTCTTTTGGGCTGAAc                                  <  1:710125/251‑1 (MQ=255)                                                                                                                                                                                                      cGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTATGACTACGCACCGTTTTACCCTTACGGCTGGGGATTTTCGTCCCGCTTCTTTTGGGCTGAAc                                  <  1:635785/251‑1 (MQ=255)                                                                                                                                                                                                      cGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTATGACTACGCACCGTTTTACCCTTACGGCTGGGGATTTTCGTCCCGCTTCTTTTGGGCTGAAc                                  <  1:354664/251‑1 (MQ=255)                                                                                                                                                                                                      cGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTATGACTACGCACCGTTTTACCCTTACGGCTGGGGATTTTCGTCCCGCTTCTTTTGGGCTGAAc                                  <  1:788642/251‑1 (MQ=255)                                                                                                                                                                                                           aTTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTATGACTACGCACCGTTTTACCCTTACGGCTGGGGAt                                                             <  1:231079/219‑1 (MQ=255)                                                                                                                                                                                                           aTTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTATGACTACGCACCGTTTTACCCTTACGGCTGGGGATTTTCGTCCCGCTTCTTTTGGGCTGAACcgcg                              >  1:462922/1‑250 (MQ=255)                                                                                                                                                                                                            tttAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTATGACTACGCACCGTTTTACCCTTACGGCTGGGGATTTTCGTCCCGCTTCTTTTGGGCTGAACcgcgcg                            >  1:17181/1‑251 (MQ=255)                                                                                                                                                                                                                   ggCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTgacg                                                                                                       >  1:2053/1‑169 (MQ=255)                                                                                                                                                                                                                    gCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAAt                                                                                                                                                                             <  1:70874/98‑1 (MQ=255)                                                                                                                                                                                                                    gCTTTGTGGATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGa                                                                                                                                        <  1:588986/135‑1 (MQ=255)                                                                                                                                                                                                                            gATCCGGAAAATCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTATGACTACGCACCGTTTTACCCTTACGGCTGGGGATTTTCGTCCCGCTTCTTTTGGGCTGAACCGCGCGTGCGCTATAATTtat             <  1:19452/250‑1 (MQ=255)                                                                                                                                                                                                                                      aTCTGAAAGACCTGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTATGACTACGCACCGTTTTACCCTTACGGCTGGGGATTTTCGTCCCGCTTCTTTTGGGCGGAACCGCGCGTGCGCTATAATTTATATTAATTAAcc  <  1:371782/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               AAACAGTTCGAAATGTTAAATCTGAAAAATGTGCAGGCGCAGGATTACGATTGCCGCTGCCTGAAAGTGCGCTTGGGCGGCAAAGTGGTTTCCGCACAGGCGTTGAAAGATAAAACCAAAATTGAAGTATTGAATCAAACGGTGGCGTATTTTTCCGCGAAACCTATCGTGGATAGCTATAGCAACGGACGTTTTATCGCCTATTTAAACGGCTTTGTGGATCCGGAAAATCTGAAAGACCAGTATGTGACAGTCGGCGGAGTGCTGAGCGGCAAAGAGCAGGGCAAAATTGATCAGGCGGATTACAATTATCCCGTGGTTCAAGCCGATCAATACCGTATTTGGAAGCTGGTGAAAGAATATTATTACGACCCTGACGACATTTATGACTACGCACCGTTTTACCCTTACGGCTGGGGATTTTCGTCCCGCTTCTTTTGGGCTGAACCGCGCGTGCGCTATAATTTATATTAATTAACC  >  1/529348‑529827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑