| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 528,601 | A→G | Q31R (CAA→CGA) | unknown → | tRNA threonylcarbamoyladenosine biosynthesis protein TsaB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 528,601 | 0 | A | G | 98.8% | 216.5 / ‑5.5 | 81 | Q31R (CAA→CGA) | unknown | tRNA threonylcarbamoyladenosine biosynthesis protein TsaB |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (44/36); total (45/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.39e-01 | |||||||||||
ATCGCGGCGTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACTGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACAATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCGCCAATTTGCCGGTGATTCCCGTGTCAAATTTAGCCGCCATGGCGCAAGCCGCTTATGTGCAATATCA > 1/528356‑528838 | aTCGCGGCGTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCg > 1:692620/1‑251 (MQ=255) cggcgTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGcac < 1:583111/250‑1 (MQ=255) cggcgTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGcac < 1:16807/250‑1 (MQ=255) cgTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAGGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACg < 1:538509/251‑1 (MQ=255) cgTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGTGCATTTGGAACGATTCGCACAACg < 1:756061/251‑1 (MQ=255) gATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACa > 1:121458/1‑251 (MQ=255) tattTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATAc < 1:10218/251‑1 (MQ=255) tGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCaaa > 1:145746/1‑251 (MQ=255) gTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTg > 1:792645/1‑250 (MQ=255) ttGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATATGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGccc < 1:303682/251‑1 (MQ=255) cgcCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGAt > 1:731515/1‑251 (MQ=255) cccTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGa > 1:93823/1‑251 (MQ=255) cccTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGa > 1:50020/1‑251 (MQ=255) aTTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAg < 1:384854/251‑1 (MQ=255) tACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCttt > 1:197925/1‑251 (MQ=255) ggATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAAc > 1:180295/1‑169 (MQ=255) aaGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAAc < 1:743099/158‑1 (MQ=255) aaGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTgg > 1:836709/1‑236 (MQ=255) gTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCggg < 1:828818/242‑1 (MQ=255) gTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGcac < 1:251972/144‑1 (MQ=255) gTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAAc > 1:891925/1‑210 (MQ=255) gTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGt < 1:713200/220‑1 (MQ=255) tACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGc < 1:532969/250‑1 (MQ=255) aTTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTTCTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCGCAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGt < 1:14235/215‑1 (MQ=255) aTTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGttt > 1:843492/1‑251 (MQ=255) ccTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGttttg > 1:349138/1‑249 (MQ=255) ccTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTa > 1:803812/1‑250 (MQ=255) ccTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAAATAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCCGGccgcttta > 1:162797/1‑243 (MQ=255) caacaaTCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGgtg > 1:81416/1‑251 (MQ=255) caacaaTCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACAATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGGGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGgtg > 1:660871/1‑251 (MQ=255) caatAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAAt > 1:574729/1‑251 (MQ=255) caatAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAAt > 1:588422/1‑251 (MQ=255) gaaTCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGccc < 1:603746/143‑1 (MQ=255) aaTCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGc < 1:74404/250‑1 (MQ=255) aaTCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCa < 1:838353/251‑1 (MQ=255) tCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCgg > 1:195410/1‑205 (MQ=255) cAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTg > 1:891647/1‑251 (MQ=255) gATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGt > 1:185780/1‑251 (MQ=255) aTGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGtt > 1:541306/1‑251 (MQ=255) aaaaTGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCg < 1:323361/251‑1 (MQ=255) aaaTGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGt < 1:392412/251‑1 (MQ=255) aTGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAAc > 1:630218/1‑159 (MQ=255) gTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGcc > 1:146636/1‑119 (MQ=255) cacaTTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGTCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGAGGATGCTTTGGTTTTCGGGCGCGGGCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGATGGCGTTCGgcg > 1:657647/1‑251 (MQ=255) caTTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAAc > 1:78758/1‑153 (MQ=255) caTTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGaa < 1:589995/213‑1 (MQ=255) gCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGc > 1:414679/1‑161 (MQ=255) gCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCa > 1:77770/1‑187 (MQ=255) cTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAG‑AAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAAc < 1:589565/143‑1 (MQ=255) acggCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCGCCAATTTGCCGGTGATTcc < 1:20090/248‑1 (MQ=255) cggCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCGCCAATTTGCCGGTGATTcc > 1:157729/3‑250 (MQ=255) ggCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCGCCAATTTGCCGGTGATTCCCg > 1:165634/2‑251 (MQ=255) aGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAggg < 1:245529/208‑1 (MQ=255) gTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCGCCAATTTGCCGGTGATTCCCGTGTCAAATTTAgc > 1:574276/1‑250 (MQ=255) gTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGaa > 1:247163/1‑175 (MQ=255) gTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGGTGGCGTTCGGCGCCAATTTGCCGGTGATTCCCGTGTCAAATTTAGCCGCCAt > 1:343114/1‑251 (MQ=255) gCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGgcg < 1:678677/143‑1 (MQ=255) gCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCGCCAATTTGCCGGTGATTCCCGTGTCAAATTTAGCCGCCAt > 1:228230/1‑248 (MQ=255) gCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCGCCAATTTGCCGGTGATTCCCGTGTCAAATTTAGCCGCCATgg > 1:32766/1‑250 (MQ=255) gCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCGCCAATTTGCCGGTGATTCCCGTGTCAAATTTAGCCGCCATgg > 1:405537/1‑250 (MQ=255) ttGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCGCCAATTTGCCGGTGATTCCCGTGTCAAATTTAGCCGCCATGGCGCa > 1:630774/1‑251 (MQ=255) ttGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCGCCAATTTGCCGGTGATTCCCGTGTCAAATTTAGCCGCCATGGCGCa > 1:300309/1‑251 (MQ=255) gTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCGCCAATTTGCCGGTGATTCCCGTGTCAAATTTAGCCGCCATGgcg > 1:62231/1‑247 (MQ=255) gcgGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTAcc < 1:790043/152‑1 (MQ=255) gTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATc < 1:519570/161‑1 (MQ=255) gTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCGCCAATTTGCCGGTGATTCCCGTGTCAAATTTAGCCGCCATGGCGCAAGCCGCTTa < 1:205518/250‑1 (MQ=255) gTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCGCCAATTTGCCGGTGATTCCCGTGTCAAATTTAGCCGCCATGGCGCAAGCCGCTTa < 1:333111/250‑1 (MQ=255) gTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCGCCAATTTGCCGGTGATTCCCGTGTCAAATTTAGCCGCCATGGCGCAAGCCGCTTAt > 1:470330/1‑251 (MQ=255) gTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCGCCAATTTGCCGGTGATTCCCGTGTCAAATTTAGCCGCCATGGCGCAAGCCGCTTAt > 1:276540/1‑251 (MQ=255) gTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGGGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCGCCAATTTGCCGGTGATTCCCGTGTCAAATTTAGCCGCCATGGCGCAAGCCGCTTAt > 1:108854/1‑251 (MQ=255) gTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTCTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCGCCAATTTGCCGGTGATTCCCGTGTCAAATTTAGCCGCCATGGCGCAAGCCGCTTAt > 1:605515/1‑251 (MQ=255) tGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCGCCAATTTGCCGGTGATTCCCGTGTCAAATTTAGCCGCCATGGCGCAAGCCGCTTAtg < 1:369822/251‑1 (MQ=255) gagagaAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCAtggt < 1:734991/64‑1 (MQ=255) gagaAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCGCCAATTTGCCGGTGATTCCCGTGTCAAATTTAGCCGCCATGGCGCAAGCCGCTTATGTGCa > 1:12539/1‑251 (MQ=255) agaAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCGCCAATTTGCCGGTGATTCCCGTGTCAAATTTAGCCGCCATGGCGCAAGCCGCTTAtg < 1:353873/246‑1 (MQ=255) agaAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCGCCAATTTGCCGGTGATTCCCGTGTCAAATTTAGCCGCCATGGCGCAAGCCGCTTATGTGc < 1:764858/249‑1 (MQ=255) agaAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCGCCAATTTGCCGGTGATTCCCGTGTCAAATTTAGCCGCCATGGCGCAAGCCGCTTATGTGc < 1:711978/249‑1 (MQ=255) agaAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCGCCAATTTGCCGGTGATTCCCGTGTCAAATTTAGCCGCCATGGCGCAAGCCGCTTATGTGc < 1:675286/249‑1 (MQ=255) agaAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCGCCAATTTGCCGGTGATTCCCGTGTCAAATTTAGCCGCCATGGCGCAAGCCGCTTATGTGc < 1:662165/249‑1 (MQ=255) agaAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCGCCAATTTGCCGGTGATTCCCGTGTCAAATTTAGCCGCCATGGCGCAAGCCGCTTATGTGc < 1:588833/249‑1 (MQ=255) cgcATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCGCCAATTTGCCGGTGATTCCCGTGTCAAATTTAGCCGCCATGGCGCAAGCCGCTTATGTGCAATATCa < 1:606265/250‑1 (MQ=255) | ATCGCGGCGTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACTGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACAATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCGCCAATTTGCCGGTGATTCCCGTGTCAAATTTAGCCGCCATGGCGCAAGCCGCTTATGTGCAATATCA > 1/528356‑528838 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |