Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	528,542	T→G	T11T (ACT→ACG)	unknown →	tRNA threonylcarbamoyladenosine biosynthesis protein TsaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	528,542	0	T	G	100.0%	214.5 / NA	77	T11T (ACT→ACG)	unknown	tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (42/35);  total (42/35)

TCCTGAAGCGGTGATTACTTTAAAACAGGGCGTCGGGCGTTTAATTCGTGACGTCACCGATCGCGGCGTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACTGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACAATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCG  >  1/528297‑528771                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                      tCCTGAAGCGGTGATTACTTTAAAACAGGGCGTCGGGCGTTTAATTCGTGACGTCACCGATCGCGGCGTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTa                                                                                                                                                                                                                                   >  1:306454/1‑250 (MQ=255)    tGAAGCGGTGATTACTTTAAAACAGGGCGTCGGGCGTTTAATTCGTGACGTCACCGATCGCGGCGTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACCTGCTTTAGATACGGCTACAGa                                                                                                                                                                                                                               <  1:583343/251‑1 (MQ=255)             gATTACTTTAAAACAGGGCGTCGGGCGTTTAATTCGTGACGTCACCGATCGCGGCGTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAg                                                                                                                                                                                                                                >  1:694030/1‑241 (MQ=255)                  cTTTAAAACAGGGCGTCGGGCGTTTAATTCGTGACGTCACCGATCGCGGCGTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTg                                                                                                                                                                                                                 >  1:340523/1‑251 (MQ=255)                    ttAAAACAGGGCGTCGGGCGTTTAATTCGTGACGTCACCGATCGCGGCGTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCa                                                                                                                                                                                                               >  1:887855/1‑251 (MQ=255)                    ttAAAACAGGGCGTCGGGCGTTTAATTCGTGACGTCACCGATCGCGGCGTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCa                                                                                                                                                                                                               <  1:274435/251‑1 (MQ=255)                      aaaaCAGGGCGTCGGGCGTTTAATTCGTGACGTCACCGATCGCGGCGTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTc                                                                                                                                                                                                                      >  1:572569/1‑242 (MQ=255)                       aaaCAGGGCGTCGGGCGTTTAATTCGTGACGTCACCGATCGCGGCGTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTg                                                                                                                                                                                                            >  1:22205/1‑251 (MQ=255)                             ggCGTCGGGCGTTTAATTCGTGACGTCACCGATCGCGGCGTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTa                                                                                                                                                                                                         <  1:884878/248‑1 (MQ=255)                              gCGTCGGGCGTTTAATTCGTGACGTCACCGATCGCGGCGTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTAcgc                                                                                                                                                                                                      <  1:795499/250‑1 (MQ=255)                               cGTCGGGCGTTTAATTCGTGACGTCACCGATCGCGGCGTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCgg                                                                                                                                                                                                    <  1:68261/251‑1 (MQ=255)                                      cGTTTAATTCGTGACGTCACCGATCGCGGCGTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGgagag                                                                                                                                                                                             <  1:514210/251‑1 (MQ=255)                                        tttAATTCGTGACGTCACCGATCGCGGCGTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGgagaga                                                                                                                                                                                            <  1:234860/250‑1 (MQ=255)                                        tttAATTCGTGACGTCACCGATCGCGGCGTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGgagaga                                                                                                                                                                                            <  1:463895/250‑1 (MQ=255)                                        tttAATTCGTGACGTCACCGATCGCGGCGTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGaa                                                                                                                                                                                           <  1:264449/251‑1 (MQ=255)                                        tttAATTCGTGACGTCACCGATCGCGGCGTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGaa                                                                                                                                                                                           <  1:307335/251‑1 (MQ=255)                                         ttAATTCGTGACGTCACCGATCGCGGCGTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGaaa                                                                                                                                                                                          <  1:3055/251‑1 (MQ=255)                                             ttCGTGACGTCACCGATCGCGGCGTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAgcg                                                                                                                                                                                       >  1:474727/1‑250 (MQ=255)                                                   aCGTCACCGATCGCGGCGTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGgagaga                                                                                                                                                                                            >  1:516139/1‑239 (MQ=255)                                                   aCGTCACCGATCGCGGCGTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGgagaga                                                                                                                                                                                            <  1:305276/239‑1 (MQ=255)                                                     gTCACCGATCGCGGCGTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTAcg                                                                                                                                                                                                       >  1:497049/1‑226 (MQ=255)                                                            aTCGCGGCGTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCg                                                                                                                                                                       >  1:692620/1‑251 (MQ=255)                                                                cggcgTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGcac                                                                                                                                                                    <  1:16807/250‑1 (MQ=255)                                                                cggcgTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGcac                                                                                                                                                                    <  1:583111/250‑1 (MQ=255)                                                                   cgTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAGGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACg                                                                                                                                                                <  1:538509/251‑1 (MQ=255)                                                                   cgTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGTGCATTTGGAACGATTCGCACAACg                                                                                                                                                                <  1:756061/251‑1 (MQ=255)                                                                       gtgATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAg                                                                                                                                                                                                                                >  1:645830/1‑183 (MQ=255)                                                                         gATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACa                                                                                                                                                          >  1:121458/1‑251 (MQ=255)                                                                            tattTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATAc                                                                                                                                                       <  1:10218/251‑1 (MQ=255)                                                                                tGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCaaa                                                                                                                                                   >  1:145746/1‑251 (MQ=255)                                                                                          gTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTg                                                                                                                                          >  1:792645/1‑250 (MQ=255)                                                                                            ttGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATATGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGccc                                                                                                                                       <  1:303682/251‑1 (MQ=255)                                                                                                     cgcCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGAt                                                                                                                              >  1:731515/1‑251 (MQ=255)                                                                                                       cccTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCgg                                                                                                                                                                                                    >  1:519715/1‑179 (MQ=255)                                                                                                       cccTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGa                                                                                                                            >  1:93823/1‑251 (MQ=255)                                                                                                       cccTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGa                                                                                                                            >  1:50020/1‑251 (MQ=255)                                                                                                                                          aTTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAg                                                                                         <  1:384854/251‑1 (MQ=255)                                                                                                                                                    tACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCttt                                                                               >  1:197925/1‑251 (MQ=255)                                                                                                                                                          ggATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAAc                                                                                                                                                           >  1:180295/1‑169 (MQ=255)                                                                                                                                                                     aaGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAAc                                                                                                                                                           <  1:743099/158‑1 (MQ=255)                                                                                                                                                                     aaGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTgg                                                                             >  1:836709/1‑236 (MQ=255)                                                                                                                                                                       gTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCggg                                                                     <  1:828818/242‑1 (MQ=255)                                                                                                                                                                          gTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGcac                                                                                                                                                                    <  1:251972/144‑1 (MQ=255)                                                                                                                                                                          gTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAAc                                                                                                  >  1:891925/1‑210 (MQ=255)                                                                                                                                                                          gTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGt                                                                                        <  1:713200/220‑1 (MQ=255)                                                                                                                                                                           tACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGc                                                         <  1:532969/250‑1 (MQ=255)                                                                                                                                                                             cAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAg                                                                                                                                                                                                                             <  1:445858/84‑1 (MQ=255)                                                                                                                                                                               aTTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTTCTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCGCAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGt                                                                                        <  1:14235/215‑1 (MQ=255)                                                                                                                                                                               aTTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGttt                                                    >  1:843492/1‑251 (MQ=255)                                                                                                                                                                                  ccTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTg                                                                                                                                                                                                                 >  1:460700/1‑91 (MQ=255)                                                                                                                                                                                  ccTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGttttg                                                  >  1:349138/1‑249 (MQ=255)                                                                                                                                                                                  ccTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTa                                                  >  1:803812/1‑250 (MQ=255)                                                                                                                                                                                  ccTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAAATAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCCGGccgcttta                                                  >  1:162797/1‑243 (MQ=255)                                                                                                                                                                                       caacaaTCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGgtg                                            >  1:81416/1‑251 (MQ=255)                                                                                                                                                                                       caacaaTCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACAATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGGGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGgtg                                            >  1:660871/1‑251 (MQ=255)                                                                                                                                                                                              caatAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAAt                                     >  1:574729/1‑251 (MQ=255)                                                                                                                                                                                              caatAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAAt                                     >  1:588422/1‑251 (MQ=255)                                                                                                                                                                                                        gaaTCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGccc                                                                                                                                       <  1:603746/143‑1 (MQ=255)                                                                                                                                                                                                         aaTCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGc                           <  1:74404/250‑1 (MQ=255)                                                                                                                                                                                                         aaTCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCa                          <  1:838353/251‑1 (MQ=255)                                                                                                                                                                                                           tCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCgg                                                                      >  1:195410/1‑205 (MQ=255)                                                                                                                                                                                                            cAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTg                       >  1:891647/1‑251 (MQ=255)                                                                                                                                                                                                              gAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCgg                                                                                                                                                                                                    <  1:855314/76‑1 (MQ=255)                                                                                                                                                                                                                     gATGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGt              >  1:185780/1‑251 (MQ=255)                                                                                                                                                                                                                      aTGAAAAATGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGtt             >  1:541306/1‑251 (MQ=255)                                                                                                                                                                                                                          aaaaTGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCg         <  1:323361/251‑1 (MQ=255)                                                                                                                                                                                                                           aaaTGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGt        <  1:392412/251‑1 (MQ=255)                                                                                                                                                                                                                             aTGTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAAc                                                                                                  >  1:630218/1‑159 (MQ=255)                                                                                                                                                                                                                               gTCACATTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGcc                                                                                                                                        >  1:146636/1‑119 (MQ=255)                                                                                                                                                                                                                                 cacaTTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGTCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGAGGATGCTTTGGTTTTCGGGCGCGGGCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGATGGCGTTCGgcg  >  1:657647/1‑251 (MQ=255)                                                                                                                                                                                                                                   caTTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAAc                                                                                                  >  1:78758/1‑153 (MQ=255)                                                                                                                                                                                                                                   caTTACTTGCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGaa                                      <  1:589995/213‑1 (MQ=255)                                                                                                                                                                                                                                           gCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGgagaga                                                                                                                                                                                            >  1:265508/1‑55 (MQ=255)                                                                                                                                                                                                                                           gCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGaa                                                                                                                                                                              <  1:306342/69‑1 (MQ=255)                                                                                                                                                                                                                                           gCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGc                                                                                  >  1:414679/1‑161 (MQ=255)                                                                                                                                                                                                                                           gCTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCa                                                        >  1:77770/1‑187 (MQ=255)                                                                                                                                                                                                                                            cTTTAGATACGGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAG‑AAGCGCATTTGGAACGATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAAc                                                                                                  <  1:589565/143‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                      TCCTGAAGCGGTGATTACTTTAAAACAGGGCGTCGGGCGTTTAATTCGTGACGTCACCGATCGCGGCGTGGTGATTATTTGCGATAATCGTTTGGTGATGCGCCCTTACGGTGAAACCTTTTTGCGCAGTTTGCCGAATTTTAAACGTACGCGGGATTTGCACAAAGTGGTACAATTCCTCACAACAATCAATAAAGAAGAATCAGAATAACGATGAAAAATGTCACATTACTTGCTTTAGATACTGCTACAGAAGCTTGTTCTGTTGCATTGTTACGCGGTGGAGAGAAAGCGCATTTGGAACAATTCGCACAACGTGAACATACCAAACATATTTTGCCCATGGTGGATGAAATTCTGGCGCAGGCGGGAATTAAACTCTATCAAGTGGATGCTTTGGTTTTCGGGCGCGGCCCGGGCAGTTTTACCGGTGTTCGAATCGGTGCCGGCATTGCGCAAGGGTTGGCGTTCGGCG  >  1/528297‑528771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑