| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 519,267 | A→G | A88A (GCT→GCC) | unknown ← | Acyl‑CoA thioesterase II (EC 3.1.2.‑) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 519,267 | 0 | A | G | 98.6% | 177.0 / NA | 69 | A88A (GCT→GCC) | unknown | Acyl‑CoA thioesterase II (EC 3.1.2.‑) |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (36/32); total (37/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAACGGGTTATTCAGGTATTTGGTGCGAATATCAAAGGGGCGTTCCGCCGTAAATTTATCGCGCACCGCTTCCGGAATCATTTGCGCCATTTGCTGGATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATAGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGAATGTAAAAAGCGATCTGTCGGCGCCACCTGAATGGCGGCAGAAAGGGCTTGCGCCACGACTTGTCCGCCGAAAACCTGACGGAAGCCTAAATCCTGACTTTCGCCGCGAAAGAGTAAATCGTCGATTTTTTCCAGTTTTAACAGGT > 1/519027‑519505 | gAACGGGTTATTCAGGTATTTGGTGCGAATATCAAAGGGGCGTTCCGCCGTAAATTTATCGCGCACCGCTTCCGGAATCATTTGCGCCATTTGCTGGATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGGAAt > 1:176218/1‑251 (MQ=255) aCGGGTTATTCAGGTATTTGGTGCGAATATCAAAGGGGCGTTCCGCCGTAAATTTATCGCGCACCGCTTCCGGAATCATTTGCGCCATTTGCTGGATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAAttt < 1:853044/251‑1 (MQ=255) ttATTCAGGTATTTGGTGCGAATATCAAAGGGGCGTTCCGCCGTAAATTTATCGCGCACCGCTTCCGGAATCATTTGCGCCATTTGCTGGATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGc > 1:468732/1‑250 (MQ=255) ggTATTTGGTGCGAATATCAAAGGGGCGTTCCGCCGTAAATTTATCGCGCACCGCTTCCGGAATCATTTGCGCCATTTGCTGGATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGGGAAATTTTTGCCTTcgggg > 1:710956/1‑248 (MQ=255) ggTATTTGGTGCGAATATCAAAGGGGCGTTCCGCCGTAAATTTATCGCGCACCGCTTCCGGAATCATTTGCGCCATTTGCTGGATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGGGAAATTTTTGCCTTcgcgg > 1:384568/1‑250 (MQ=255) aTTTGGTGCGAATATCAAAGGGGCGTTCCGCCGTAAATTTATCGCGCACCGCTTCCGGAATCATTTGCGCCATTTGCTGGATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGCGGGGGAAATTTTTG‑CTTCCCG‑TAGa > 1:18861/1‑251 (MQ=255) cGAATATCAAAGGGGCGTTCCGCCGTAAATTTATCGCGCACCGCTTCCGGAATCATTTGCGCCATTTGCTGGATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCcac < 1:217117/251‑1 (MQ=255) tatCAAAGGGGCGTTCCGCCGTAAATTTATCGCGCACCGCTTCCGGAATCATTTGCGCCATTTGCTGGATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATTTGGTAAATCGGTGATTTGTGCTGAATAGCTTTGGAGCGCATGGC‑GGTGTGATTTTTGCCGTCGCGATGGAATTTcacgtca > 1:487081/1‑245 (MQ=255) tatCAAAGGGGCGTTCCGCCGTAAATTTATCGCGCACCGCTTCCGGAATCATTTGCGCCATTTGCTGGATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCAcatca > 1:14380/1‑251 (MQ=255) aaaGGGGCGTTCCGCCGTAAATTTATCGCGCACCGCTTCCGGAATCATTTGCGCCATTTGCTGGATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATaaa < 1:41355/251‑1 (MQ=255) ggggCGTTCCGCCGTAAATTTATCGCGCACCGCTTCCGGAATCATTTGCGCCATTTGCTGGATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAAATTCTGCCTTCGCG‑TAGAATTTTCACATCATaaa > 1:852758/1‑248 (MQ=255) ggggCGTTCCGCCGTAAATTTATCGCGCACCGCTTCCGGAATCATTTGCGCCATTTGCTGGATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGGGGAATTTCTGCCTTCGCG‑GAGAATTTCCACATCATAAATGa > 1:528234/1‑251 (MQ=255) gggCGTTCCGCCGTAAATTTATCGCGCACCGCTTCCGGAATCATTTGCGCCATTTGCTGGATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATg < 1:791930/249‑1 (MQ=255) ggCGTTCCGCCGTAAATTTATCGCGCACCGCTTCCGGAATCATTTGCGCCATTTGCTGGATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCTTTCGCG‑TAGAATTTCCACATCATAAATGA‑t < 1:161377/250‑1 (MQ=255) ggCGTTCCGCCGTAAATTTATCGCGCACCGCTTCCGGAATCATTTGCGCCATTTGCTGGATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑t < 1:137274/250‑1 (MQ=255) tCCGCCGTAAATTTATCGCGCACCGCTTCCGGAATCATTTGCGCCATTTGCTGGATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGata < 1:610651/251‑1 (MQ=255) cgTAAATTTATCGCGCACCGCTTCCGGAATCATTTGCGCCATTTGCTGGATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGc < 1:826511/251‑1 (MQ=255) cgTAAATTTATCGCGCACCGCTTCCGGAATCATTTGCGCCATTTGCTGGATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGc < 1:665183/251‑1 (MQ=255) cACCGCTTCCGGAATCATTTGCGCCATTTGCTGGATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGCAAAACGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGGGAAAATTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATAA‑ACGGATATTTGCTGTCcccccggcgt > 1:126602/1‑244 (MQ=255) cGCTTCCGGAATCATTTGCGCCATTTGCTGGATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTaaaa < 1:747642/251‑1 (MQ=255) cATTTGCGCCATTTGCTGGATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGGGAAATTTTTGCCTTCGCG‑TAGAATTTTCACATCATAAATGG‑TCGGGTATTGGCTGGCGCCCGGGGGTaaaa > 1:353775/1‑239 (MQ=255) cgcCATTTGCTGGATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGaa < 1:113600/250‑1 (MQ=255) ccATTTGCTGGATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTTCACATCATAAATTA‑TCGGGTATTg > 1:538276/1‑211 (MQ=255) aTTTGCTGGATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGAATGTaa < 1:473138/251‑1 (MQ=255) cTGGATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTTCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGAATGTAAAAAg < 1:427685/250‑1 (MQ=255) ggATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTTTGCCTTCGCG‑CAGAATTTCCACATCATAAATTA‑TCGGATATTGGGTGTCGCCCGGGGCTAAAAAATACGCCTGGCAAGAATGTAAAAAGCGa > 1:751162/1‑251 (MQ=255) tttCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGAATGTAAAAAGCGATCTGTCGGCGcc < 1:867510/251‑1 (MQ=255) ttCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTTCTGAATGGTCTTGAACCGCATAGC‑CGGGGAATTTTTCCCTTCGCG‑GAGCACTTTCACCTCATTACTGATTCGAATTTTGCCT‑TCTCCCGGGGCTAAAAAATACGCCTGGGAAGAAAGTAAAAAGGGCTCTGTCCGCGcc > 1:545007/1‑250 (MQ=255) tCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATg > 1:680734/1‑177 (MQ=255) ccGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTaaaa < 1:462636/207‑1 (MQ=255) gACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGGGAAATTTTTGCCCTCGCG‑GAGAAttttc > 1:436719/1‑160 (MQ=255) gACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGGGAAATTTCTGCCTTCGCG‑TAGAATTTTCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGGTAAAAAAAACGCATGGCAAGAATGTAAAAAGCGAACTGTCGGCGCCAc > 1:7291/1‑248 (MQ=255) aCATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTTTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTCAAAAATACGCATGGCAAGAATGTAAAAAGCGATCTGTCGGCGCCACCTGa > 1:3143/1‑251 (MQ=255) tGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCcac > 1:72768/1‑160 (MQ=255) gAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGatat < 1:658045/178‑1 (MQ=255) gCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGAATGTAAAAAGCGATCTGTCGGCGCCACCTGAATGGCGGCAGAAAggg < 1:531386/250‑1 (MQ=255) ccTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGAATGTAAAAAGCGATCTGTCGGCGCCACCTGAATGGCGGCAGAAAGGGCt < 1:7819/251‑1 (MQ=255) cTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTTTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGAATGTAAAAAGCGATCTGTCGGCGCCACCTGAATGGCGGGAGAAAGGGCtt > 1:300021/1‑251 (MQ=255) cTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGAATGTaaa < 1:15865/203‑1 (MQ=255) cTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGGGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCCGGGCTAAAAAAAAAGCAAGGCaaaaaattaaaaaa > 1:176908/1‑194 (MQ=255) aCTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATg < 1:878909/136‑1 (MQ=255) cTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGGGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCT‑AAAAATACGCATGGCAAGAATGTAAAAAACGATCTGTCGGCGCCACCTGAATGGCGGCAGAAAGGGCTTGCGCCACGACTTGTccgccg > 1:467037/1‑251 (MQ=255) ttGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGAATGTAAAAAGCGATCTGTCGGCGCCACCTGAAt < 1:33538/213‑1 (MQ=255) gCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGGAATTTTTGCCTTCGCG‑TAGAATTTTCACAACATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGAATGTAAAAAGCGATCTGTCGGCGCCACCTGAATGGGGGCAGAAAGGGGTTGCGCCACGAATTTTCCGCCGAAAAc > 1:768762/1‑250 (MQ=255) gCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGAATGTAAAAAGCGATCTGTCGGCGCCACCCGAAAGGCGGCAGAAAGGGCTTGCGCCACGACTTGTCCGCCGAAAAc > 1:9154/1‑250 (MQ=255) gCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGGAAATCGGGGATTTGTGCTGAATGGCCTTGACGCGCATGGC‑GGTGGAACTTCTTCCTTCCCG‑TAAAATTTCCACCTCAAAAATGA‑CCGGGCATTGGGTCGCGCCCCGGGGTAAAAAATATGCATGGCGAGAAAGTAAAAAACGGTCTGTCGGCGCCCCCCTAAAGGGGGGAAAAAGGGCTTGTGCCAcccgttgtcccgcggaaac > 1:816576/1‑232 (MQ=255) cTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATg < 1:276438/127‑1 (MQ=255) cTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGc < 1:477646/165‑1 (MQ=255) ggTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTTTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGGCGCCCGGGGCTAAAAAATACGCAAGGCAAGAATGTAAAAAGCGAACTGGCGGCGCCACCTGAAAGGCGGCAGAAAGGGCTTGCGCCCCGACTTGTCCGCCGAAAACCTGACGGAAGCCTAAATcc > 1:271104/1‑250 (MQ=255) ggTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCcac < 1:62859/99‑1 (MQ=255) gTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGatat > 1:220340/1‑117 (MQ=255) gTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGAATGTAAAAAGCGATCTGTCGGCGCCACCTGAATGGCGGCAGAAAGGGCTTGCGCCACGACTTGTc < 1:735460/220‑1 (MQ=255) tGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGAATGTAAAAAGCGATCTGTCGGCGCCACCTGAATGGCGGCAGAAAGGGCTTGCGCCACGACTTGTCCGCCGAAAACCTGACGGAAGCCTAAATCCTGACt < 1:211085/251‑1 (MQ=255) gAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGc < 1:872600/141‑1 (MQ=255) agGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGaa < 1:766815/146‑1 (MQ=255) gCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGaa > 1:141681/1‑144 (MQ=255) gATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATg > 1:536521/1‑91 (MQ=255) gATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGAATGTAAAAAGCGATCTGTCGGCGCCACCTGAATGGCGGCAGAAAGGGCTTGCGCCACGACTTGTCCGCCGAAAACCTGACGGAAGCCTAAATCCTGACTTTCGCCGCGAAaga > 1:879962/1‑251 (MQ=255) gtgGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGaa < 1:4868/136‑1 (MQ=255) gtgGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGAATGTAAAAAGCGATCTGTCGGCGCCACCTGAATGGCGGCAGAAAGGGCTTGCGCCACGACTTGTCCGCCGAAAACCTGACGGAAGCCTAAATCCTGACTTTCGCCGCGAAAGAGTa > 1:467503/1‑251 (MQ=255) aaaTCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGAATGTAAAAAGCGATCTGTCGGCGCCACCTGAATGGCGGCAGAAAGGGCTTGCGCCACGACTTGTCCGCCGAAAACCTGACGGAAGCCTAAATCCTGACTTTCGCCGCGAAAGAGTAAAtcg < 1:779698/251‑1 (MQ=255) aaaTCGGTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGAATGTAAAAAGCGATCTGTCGGCGCCACCTGAATGGCGGCAGAAAGGGCTTGCGCCACGACTTGTCCGCCGAAAACCTGACGGAAGCCTAAATCCTGACTTTCGCCGCGAAAGAGTAAAtcg < 1:553815/251‑1 (MQ=255) ggTGATTTGTGCTGAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGAATGTAAAAAGCGATCTGTCGGCGCCACCTGAATGGCGGCAGAAAGGGCTTGCGCCACGACTTGTCCGCCGAAAACCTGACGGAAGCCTAAATCCTGACTTTCGCCGCGAAAGAGTAAATCGTCGAt > 1:837177/1‑251 (MQ=255) gAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGAATGTAAAAAg < 1:432374/122‑1 (MQ=255) gAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGAATGTAAAAAGCGATCTGTCGGCGCCACCTGAATGGCGGCAGAAAGGGCTTGCGCCACGACTTGTCCGCCGAAAACCTGACGGAAGCCTAAATCCTGACTTTCGCCGCGAAAGAGTAAATCGTCGATTTTTTCCAGtttt > 1:662702/1‑251 (MQ=255) gAATGGCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGAATGTAAAAAGCGATCTGTCGGCGCCACCTGAATGGCGGCAGAAAGGGCTTGCGCCACGACTTGTCCGCCGAAAACCTGACGGAAGCCTAAATCCTGACTTTCGCCGCGAAAGAGTAAATCGTCGATTTTTTCCAGtttt > 1:865451/1‑251 (MQ=255) gCTTTGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGAATGTAAAAAGCGATCTGTCGGCGCCACCTGAATGGCGGCAGAAAGGGCTTGCGCCACGACTTGTCCGCCGAAAACCTGACGGAAGCCTAAATCCTGACTTTCGCCGCGAAAGAGTAAATCGTCGATTTTTTCCAGTTTTAACAg > 1:91279/1‑251 (MQ=255) ttGACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGAATGTAAAAAGCGATCTGTCGGCGCCACCTGAATGGCGGCAGAAAGGGCTTGCGCCACGACTTGTCCGCCGAAAACCTGACGGAAGCCTAAATCCTGACTTTCGCCGCGAAAGAGTAAATCGTCGATTTTTTCCAGTTTTAACAGGt > 1:571909/1‑250 (MQ=255) gACGCGCATGGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGc > 1:530413/1‑68 (MQ=255) | GAACGGGTTATTCAGGTATTTGGTGCGAATATCAAAGGGGCGTTCCGCCGTAAATTTATCGCGCACCGCTTCCGGAATCATTTGCGCCATTTGCTGGATCACCGAGCTTTCCGACATGAGTGCATCGGGGGCCTCCACTTTTGGCATCTCACTTTGATGCTCAAAGCCTTGCTCCAAGGTTTGGAAAGAGGCGGTGATGTGGTAAATCGGTGATTTGTGCTGAATGGCTTTGACGCGCATAGC‑GGTGAAATTTCTGCCTTCGCG‑TAGAATTTCCACATCATAAATGA‑TCGGATATTGGCTGTCGCCCGGGGCTAAAAAATACGCATGGCAAGAATGTAAAAAGCGATCTGTCGGCGCCACCTGAATGGCGGCAGAAAGGGCTTGCGCCACGACTTGTCCGCCGAAAACCTGACGGAAGCCTAAATCCTGACTTTCGCCGCGAAAGAGTAAATCGTCGATTTTTTCCAGTTTTAACAGGT > 1/519027‑519505 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |