Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 499,971 G→A G59G (GGG→GGA)  unknown → Teichoic acid export ATP‑binding protein TagH (EC 3.6.3.40)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1499,9710GA100.0% 48.3 / NA 19G59G (GGG→GGA) unknownTeichoic acid export ATP‑binding protein TagH (EC 3.6.3.40)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/9);  total (10/9)

AACAAGATGAATTTATATTGTACATATAGCTATGAATACAGAAATAATTAACGTTGAAAATGCTACTGTTCGCTTTAATAAAGCAATGGAAAATTATAGCGGCTTAAAAGACTACATTATAAAAATGCTAAAAGGAAAGTTAATGTTCCAAGAATTTTTATCATTAAATGATGTTTCTTTTAAAGTTAAAAAGGGTGAATCTTGGGGGTTGATTGGTACTAATGGCTCAGGAAAATCTACACTACTAAAACTTATCTGTGGAATTCTAAAACCATATAAAGGAAAACTTGAAGTTCATGGAAAGATTTCTCCTCTTATAGAGCTAGGAGCTGGATTTGATGGAGAATTAACGGCAAGAGAAAACATATATTTAAATGGGGCATTGTTAGGATATGATAAATCTTTAATGGATACTCATTTTGAAGAAATAATTGAAT  >  1/499764‑500200
                                                                                                                                                                                                               |                                                                                                                                                                                                                                     
aacaagaTGAATTTATATTGTACATATAGCTATGAATACAGAAATAATTAACGTTGAAAATGCTACTGTTCGCTTTAATAAAGCAATGGAAAATTATAGCGGCTTAAAAGACTACATTATAAAAATGCTAAAAGGAAAGTTAATGTTCCAAGAATTTTTATCATTAAATGATGTTTCTTTTAAAGTTAAAAAGGGTGAATCTTGGGGATTGATTGGTACTAATGGCTCAGGAAAATCTACACTACTAAAAc                                                                                                                                                                                            <  1:291642/251‑1 (MQ=255)
    agaTGAATTTATATTGTACATATAGCTATGAATACAGAAATAATTAACGTTGAAAATGCTACTGTTCGCTTTAATAAAGCAATGGAAAATTATAGCGGCTTAAAAGACTACATTATAAAAATGCTAAAAGGAAAGTTAATGTTCCAAGAATTTTTATCATTAAATGATGTTTCTTTTAAAGTTAAAAAGGGTGAATCTTGGGGATTGATTGGTACTAATGGCTCAGGAAAATCTACact                                                                                                                                                                                                    <  1:591968/239‑1 (MQ=255)
              atatTGTACATATAGCTATGAATACAGAAATAATTAACGTTGAAAATGCTACTGTTCGCTTTAATAAAGCAATGGAAAATTATAGCGGCTTAAAAGACTACATTATAAAAATGCTAAAAGGAAAGTTAATGTTCCAAGAATTTTTATCATTAAATGATGTTTCTTTTAAAGTTAAAAAGGGTGAATCTTGGGGATTGATTGGTACTAATGGCTCAGGAAAATCTACACTACTAAAACTTATCTGTGGAAtt                                                                                                                                                                              >  1:8877/1‑251 (MQ=255)
                     aCATATAGCTATGAATACAGAAATAATTAACGTTGAAAATGCTACTGTTCGCTTTAATAAAGCAATGGAAAATTATAGCGGCTTAAAAGACTACATTATAAAAATGCTAAAAGGAAAGTTAATGTTCCAAGAATTTTTATCATTAAATGATGTTTCTTTTAAAGTTAAAAAGGGTGAATCTTGGGGATTGATTGGTACTaa                                                                                                                                                                                                                         >  1:764410/1‑201 (MQ=255)
                            gCTATGAATACAGAAATAATTAACGTTGAAAATGCTACTGTTCGCTTTAATAAAGCAATGGAAAATTATAGCGGCTTAAAAGACTACATTATAAAAATGCTAAAAGGAAAGTTAATGTTCCAAGAATTTTTATCATTAAATGATGTTTCTTTTAAAGTTAAAAAGGGTGCATCTTGGGGATTGATTGGTACTAATGGCTCAGGAAAATCTacac                                                                                                                                                                                                     >  1:431690/1‑214 (MQ=255)
                              tATGAATACAGAAATAATTAACGTTGAAAATGCTACTGTTCGCTTTAATAAAGCAATGGAAAATTATAGCGGCTTAAAAGACTACATTATAAAAATGCTAAAAGGAAAGTTAATGTTCCAAGAATTTTTATCATTAAATGATGTTTCTTTTAAAGTTAAAAAGGGTGAATCTTGGGGATTGATTGGTACTAATGGCTCAGGAAAATCTACACTACTAAAACTTATCTGTGGAATTCTAAAACCATATAAAg                                                                                                                                                              >  1:678567/1‑251 (MQ=255)
                                                    gTTGAAAATGCTACTGTTCGCTTTAATAAAGCAATGGAAAATTATAGCGGCTTAAAAGACTACATTATAAAAATGCTAAAAGGAAAGTTAATGTTCCAAGAATTTTTATCATTAAATGATGTTTCTTTTAAAGTTAAAAAGGGTGAATCTTGGGGATTGATTGGTACTAATGGCTCAGGAAAATCTACACTACTAAAACTTATCTGTGGAATTCTAAAACCATATaaa                                                                                                                                                               <  1:320209/228‑1 (MQ=255)
                                                                        cTTTAATAAAGCAATGGAAAATTATAGCGGCTTAAAAGACTACATTATAAAAATGCTAAAAGGAAAGTTAATGTTCCAAGAATTTTTATCATTAAATGATGTTTCTTTTAAAGTTAAAAAGGGTGAATCTTGGGGATTGATTGGTAc                                                                                                                                                                                                                            <  1:709366/147‑1 (MQ=255)
                                                                          ttAATAAAGCAATGGAAAATTATAGCGGCTTAAAAGACTACATTATAAAAATGCTAAAAGGAAAGTTAATGTTCCAAGAATTTTTATCATTAAATGATGTTTCTTTTAAAGTTAAAAAGGGTGAATCTTGGGGATTGATTGGTACTAATGGCTCAGGAAAATCTACACTACTAAAACTTATc                                                                                                                                                                                       >  1:200644/1‑182 (MQ=255)
                                                                           taataaAGCAATGGAAAATTATAGCGGCTTAAAAGACTACATTATAAAAATGCTAAAAGGAGAGTTAATGTTCCAAGAATTCTTATCATTAAATGATGTTTCTTTTAAAGTTAAAAAGGGTGAATCTTGGGGATTGATTGGTACTAATGGCTCAGGAAAATCTACACTACTAAAACTTATCTGTGGAATTCTAAAACCATATAAAGGAAAACTTGAAGTTCATGGAAAGATTTCTCCTCTTATAGAGCTAg                                                                                                                 >  1:486153/1‑251 (MQ=255)
                                                                                       ggAAAATTATAGCGGCTTAAAAGACTACATTATAAAAATGCTAAAAGGAAAGTTAATGTTCCAAGAATTTTTATCATTAAATGATGTTTCTTTTAAAGTTAAAAAGGGTGAATCTTGGGGATTGATTGGTACTAATGGCTCAGGAAAATCTACACTACTAAAACTTATCTGTGGAATTCTAAAACCATATAAAGGAAAACTTGAAGTTCATGGAAAGATTTCTCCTCTTATAGAGCTAGGAGCTGGATTTg                                                                                                     >  1:489915/1‑251 (MQ=255)
                                                                                                                    ttATAAAAATGCTAAAAGGAAAGTTAATGTTCCAAGAATTTTTATCATTAAATGATGTTTCTTTTAAAGTTAAAAAGGGTGAATCTTGGGGATTGATTGGTACTAATGGCTCAGGAAAATCTACACTACTAAAACTTATCTGTGGAATTCTAAAACCATATAAAGGAAAACTTGAAGTTCATGGAAAGATTTCTCCTCTTATAGAGCTAGGAGCTGGATTTGATGGAGAATTAACgg                                                                                      <  1:499174/237‑1 (MQ=255)
                                                                                                                                   aaGGAAAGTTAATGTTCCAAGAATTTTTATCATTAAATGATGTTTCTTTTAAAGTTAAAAAGGGTGAATCTTGGGGATTGATTGGTACTAATGGCTCAGGAAAATCTACACTACTAAAACTTATCTGTGGAATTCTAAAACCATATAAAGGAAAACTTGAAGTTCATGGAAAGATTTCTCCTCTTATAGAGCTAGGAGCTGGATTTGATGGAGAATTAACGGCAAGAGAAAACATATATTTAAATGGGGCa                                                         >  1:523715/1‑251 (MQ=255)
                                                                                                                                          gTTAATGTTCCAAGAATTTTTATCATTAAATGATGTTTCTTTTAAAGTTAAAAAGGGTGAATCTTGGGGATTGATTGGTACTAATGGCTCAGGAAAATCTACACTACTAAAACt                                                                                                                                                                                           <  1:683162/114‑1 (MQ=255)
                                                                                                                                                                         gatgTTTCTTTTAAAGTTAAAAAGGGTGAATCTTGGGGATTGATTGGTACTAATGGCTCAGGCAAATCTACACTACTAAAACTTATCTGTGGAATTCTAAAACCATATAAAGGAAAACTTGAAGTTCATGGAAAGATTTCTCCTCTTATAGAGc                                                                                                                    <  1:422476/154‑1 (MQ=255)
                                                                                                                                                                                cTTTTAAAGTTAAAAAGGGTGAATCTTGGGGATTGATTGGTACTAATGGCTCAGGAAAATCTACACTACTAAAACTTATCTGTGGAATTCTAAAACCATATAAAGGAAAACTTGAAGTTCATGGAAAGATTTCTCCTCTTATagag                                                                                                                     >  1:606268/1‑146 (MQ=255)
                                                                                                                                                                                          tAAAAAGGGTGAATCTTGGGGATTGATTGGTACTAATGGCTCAGGAAAATCTACACTACTAAAACTTATCTGTGGAATTCTAAAACCATATAAAGGAAAACTTGAAGTTCATGGAAAGATTTCTCCTCTTATAGAGCTAGGAGCTGGATTTGATgg                                                                                                 >  1:742369/1‑156 (MQ=255)
                                                                                                                                                                                          tAAAAAGGGTGAATCTTGGGGATTGATTGGTACTAATGGCTCAGGAAAATCTACACTACTAAAACTTATCTGTGGAATTCTAAAACCATATAAAGGAAAACTTGAAGTTCATGGAAAGATTTCTCCTCTTATAGAGCTAGGAGCTGGATTTGATGGAGAATTAACGGCAAGAGAAAACATATATTTAAATGGGGCATTGTTAGGATATGATAAATCTTTAATGGATACTCATTTTGAAGAAATAATTGAAt  <  1:344322/251‑1 (MQ=255)
                                                                                                                                                                                                 ggTGAATCTTGGGGATTGATTGGTACTAATGGCTCAGGAAAATCTACACTACTAAAACTTATCTGTGGAATTCTAAAACCATATAAAGGAAAACTTGAAGTTCATGGAAAGATTTCTCCTCTTATagag                                                                                                                     <  1:210217/129‑1 (MQ=255)
                                                                                                                                                                                                               |                                                                                                                                                                                                                                     
AACAAGATGAATTTATATTGTACATATAGCTATGAATACAGAAATAATTAACGTTGAAAATGCTACTGTTCGCTTTAATAAAGCAATGGAAAATTATAGCGGCTTAAAAGACTACATTATAAAAATGCTAAAAGGAAAGTTAATGTTCCAAGAATTTTTATCATTAAATGATGTTTCTTTTAAAGTTAAAAAGGGTGAATCTTGGGGGTTGATTGGTACTAATGGCTCAGGAAAATCTACACTACTAAAACTTATCTGTGGAATTCTAAAACCATATAAAGGAAAACTTGAAGTTCATGGAAAGATTTCTCCTCTTATAGAGCTAGGAGCTGGATTTGATGGAGAATTAACGGCAAGAGAAAACATATATTTAAATGGGGCATTGTTAGGATATGATAAATCTTTAATGGATACTCATTTTGAAGAAATAATTGAAT  >  1/499764‑500200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑