Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	497,371	C→G	A233G (GCG→GGG)	unknown →	Glucose‑1‑phosphate thymidylyltransferase (EC 2.7.7.24)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	497,371	0	C	G	100.0%	125.4 / NA	47	A233G (GCG→GGG)	unknown	Glucose‑1‑phosphate thymidylyltransferase (EC 2.7.7.24)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (22/25);  total (22/25)

TCTATTGAAGAAAAACCTGTTCAACCTAAATCTAACTATGCTGTAACAGGTTTATATTTCTACGATAATCGTGTGGTCGATTTCGCGAAACAGGTGAAACCATCTGCGCGCGGTGAGCTTGAAATCACCACATTAAATGAAATGTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGCGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGCAAGTAGAAACATTAGCGAGACCAATGGTAAAAAATGAATACGGTCAATACTTGCTACGTTTAATCAACGAGGAGAAATAAATGGCAAAATTTCTGATTACAGGCGCAAACGGGCAAGTGGGCTACTGTTTAACCCAACAATTG  >  1/497139‑497609                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               tCTATTGAAGAAAAACCTGCTCAACCTAAATCTAACTATGCTGTAACAGGTTTATATTTCTACGATAATCGTGTGGTCGATTTCGCGAAACAGGTGAAACCATCTGCGCGCGGTGAGCTTGAAATCACCACATTAAATGAAATGTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCAc                                                                                                                                                                                                                              <  1:489656/251‑1 (MQ=255)      tGAAGAAAAACCTGCTCAACCTAAATCTAACTATGCTGTAACAGGTTTATATTTCTACGATAATCGTGTGGTCGATTTCGCGAAACAGGTGAAACCATCTGCGCGCGGTGAGCTTGAAATCACCACATTAAATGAAATGTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTg                                                                                                                                                                                                                         <  1:636731/251‑1 (MQ=255)                         ccTAAATCTAACTATGCTGTAACAGGTTTATATTTCTACGATAATCGTGTGGTCGATTTCGCGAAACAGGTGAAACCATCTGCGCGCGGTGAGCTTGAAATCACCACATTAAATGAAATGTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCa                                                                                                                                                                                                      <  1:238713/251‑1 (MQ=255)                                      aTGCTGTAACAGGTTTATATTTCTACGATAATCGTGTGGTCGATTTCGCGAAACAGGTGAAACCATCTGCGCGCGGTGAGCTTGAAATCACCACATTAAATGAAATGTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAgg                                                                                                                                                                                                            <  1:636793/232‑1 (MQ=255)                                      aTGCTGTAACAGGTTTATATTTCTACGATAATCGTGTGGTCGATTTCGCGAAACAGGTGAAACCATCTGCGCGCGGTGAGCTTGAAATCACCACATTAAATGAAATGTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTg                                                                                                                                                                                         >  1:137790/1‑251 (MQ=255)                                       tGCTGTAACAGGTTTATATTTCTACGATAATCGTGTGGTCGATTTCGCGAAACAGGTGAAACCATCTGCGCGCGGTGAGCTTGAAATCACCACATTAAATGAAATGTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGt                                                                                                                                                                                            >  1:519711/1‑247 (MQ=255)                                           gTAACAGGTTTATATTTCTACGATAATCGTGTGGTCGATTTCGCGAAACAGGTGAAACCATCTGCGCGCGGTGAGCTTGAAATCACCACATTAAATGAAATGTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGgaaga                                                                                                                                                                                    >  1:682858/1‑251 (MQ=255)                                                 ggTTTATATTTCTACGATAATCGTGTGGTCGATTTCGCGAAACAGGTGAAACCATCTGCGCGCGGTGAGCTTGAAATCACCACATTAAATGAAATGTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCTTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTg                                                                                                                                                                               >  1:134274/1‑250 (MQ=255)                                                 ggTTTATATTTCTACGATAATCGTGTGGTCGATTTCGCGAAACAGGTGAAACCATCTGCGCGCGGTGAGCTTGAAATCACCACATTAAATGAAATGTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGt                                                                                                                                                                                                   <  1:64321/230‑1 (MQ=255)                                                 ggTTTATATTTCTACGATAATCGTGTGGTCGATTTCGCGAAACAGGTGAAACCATCTGCGCGCGGTGAGCTTGAAATCACCACATTAAATGAAATGTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTg                                                                                                                                                                               >  1:87574/1‑250 (MQ=255)                                                             tACGATAATCGTGTGGTCGATTTCGCGAAACAGGTGAAACCATCTGCGCGCGGTGAGCTTGAAATCACCACATTAAATGAAATGTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCGCTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATgg                                                                                                                                                                  <  1:185375/251‑1 (MQ=255)                                                                 aTAATCGTGTGGTCGATTTCGCGAAACAGGTGAAACCATCTGCGCGCGGTGAGCTTGAAATCACCACATTAAATGAAATGTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGATACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATgg                                                                                                                                                                  <  1:63979/247‑1 (MQ=255)                                                                      cGTGTGGTCGATTTCGCGAAACAGGTGAAACCATCTGCGCGCGGTGAGCTTGAAATCACCACATTAAATGAAATGTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAAc                                                                                                                                                         <  1:857564/251‑1 (MQ=255)                                                                              cGATTTCGCGAAACAGGTGAAACCATCTGCGCGCGGTGAGCTTGAAATCACCACATTAAATGAAATGTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGc                                                                                                                                                 <  1:60153/251‑1 (MQ=255)                                                                              cGATTTCGCGAAACAGGTGAAACCATCTGCGCGCGGTGAGCTTGAAATCACCACATTAAATGAAATGTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGc                                                                                                                                                 <  1:468197/251‑1 (MQ=255)                                                                                             ggTGAAACCATCTGCGCGCGGTGAGCTTGAAATCACCACATTAAATGAAATGTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATgg                                                                                                                                                                  <  1:73612/219‑1 (MQ=255)                                                                                                  aaCCATCTGCGCGCGGTGAGCTTGAAATCACCACATTAAATGAAATGTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGCAAGTAGAAACa                                                                                                                                      <  1:21787/242‑1 (MQ=255)                                                                                                    ccATCTGCGCGCGGTGAGCTTGAAATCACCACATTAAATGAAATGTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGCAAGTAGAAACATTAGCGAGAcc                                                                                                                           <  1:62114/251‑1 (MQ=255)                                                                                                     cATCTGCGCGCGGTGAGCTTGAAATCACCACATTAAATGAAATGTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATgg                                                                                                                                                                  >  1:376582/1‑211 (MQ=255)                                                                                                                 gTGAGCTTGAAATCACCACATTAAATGAAATGTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGCAAGTAGAAACATTAGCGAGACCAATgg                                                                                                                      <  1:268773/243‑1 (MQ=255)                                                                                                                             tCACCACATTAAATGAAATGTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATgg                                                                                                                                                                  <  1:437834/187‑1 (MQ=255)                                                                                                                                   caTTAAATGAAATGTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGCAAGTAGAAACATTAGCGAGACCAATGGTAAAAAATGAATACGGTCAATACTTg                                                                                            >  1:351358/1‑251 (MQ=255)                                                                                                                                   caTTAAATGAAATGTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGCAAGTAGAAACATTAGCGAGACCAATGGTAAAAAATGAATACGGTCAATACTTg                                                                                            >  1:169165/1‑251 (MQ=255)                                                                                                                                      tAAATGAAATGTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGCAAGTAGAAACATTAGCGAGACCAATGGTAAAAAATGAATACGGTCAATACTTGCTa                                                                                         <  1:70140/251‑1 (MQ=255)                                                                                                                                          tgaaatgTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGCAAGTAGAAACATTAGCGAGACCAATGGTAAAAAATGAATACGGTCAATACTTGCTACGtt                                                                                     <  1:438177/251‑1 (MQ=255)                                                                                                                                                gTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGCAAGTAGAAACATTAGCGAGACCAATGGTAAAAAATGAATACGGTCAATACTTGCTa                                                                                         <  1:627013/241‑1 (MQ=255)                                                                                                                                                  aTCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGCAAGTAGAAACATTAGCGAGACCAATGGTAAAAAATGAATACGGTCAATACTTGCTACGTTTAATCaa                                                                              >  1:116855/1‑250 (MQ=255)                                                                                                                                                  aTCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGCAAGTAGAAACATTAGCGAGACCAATGGTAAAAAATGAATACGGTCAATACTTGCTACGTTTAATCaa                                                                              >  1:577402/1‑250 (MQ=255)                                                                                                                                                  aTCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGACTAACTTCTGAGCAAGTAGAAACATTAGCGAGACCAATGGTAAAAAATGAATACGGTCAATACTTGCTACGTTTAATCaa                                                                              >  1:454279/1‑250 (MQ=255)                                                                                                                                                      aaaaGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGCAAGTAGAAACATTAGCg                                                                                                                                >  1:604499/1‑196 (MQ=255)                                                                                                                                                              gTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGc                                                                                                                                                                                                                    >  1:337265/1‑104 (MQ=255)                                                                                                                                                              gTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGCAAGTAGAAACATTAGCGAGACCAATGGTAAAAAATGAATACGGTCAATACTTGCTACGTTTAATCAACGAGGAGaaataa                                                                 >  1:743540/1‑251 (MQ=255)                                                                                                                                                                 cATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGCAAGTAGAAACATTAGCGAGACCAATGGTAAAAAATGAATACGGTCAATACTTGCTACGTTTAATCAACGAGGAGAAATAAATg                                                              >  1:866352/1‑251 (MQ=255)                                                                                                                                                                 cATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGCAAGTAGAAACATCAGCGAGACCAATGGTAAAAAATGAATACGGTCAATACTTGCTACGTTTAATCAACGAGGAGAAATAAATg                                                              >  1:25307/1‑251 (MQ=255)                                                                                                                                                                            cAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGc                                                                                                                                                 >  1:474643/1‑157 (MQ=255)                                                                                                                                                                                      gTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGCAAGTAGAAACATTAGCGAGACCAATGGTAAAAAATGAATACGGTCAATACTTGCTACGTTTAATCAACGAGGAGAAATAAATGGCAAAATTTCTGATTACAgg                                          >  1:878751/1‑250 (MQ=255)                                                                                                                                                                                           ggTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGgaag                                                                                                                                                                                     <  1:167195/106‑1 (MQ=255)                                                                                                                                                                                                     ggTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGCAAGTAGAAACATTAGCGAGACCAATGGTAAAAAATGAATACGGTCAATACTTGCTACGTTTAATCAACGAGGAGAAATAAATGGCAAAATTTCTGATTACAGGCGCAAACGGGCAAGTg                          >  1:562096/1‑251 (MQ=255)                                                                                                                                                                                                      gTTAGACACAGGAACGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGCAAGTAGAAACATTAGCGAGACCAATGGTAAAAAATGAATACGGTCAATACTTGCTACGTTTAATCAACGAGGAGAAATAAATGGCAAAATTTCTGATTACAGGCGCAAACGGGCAAGTgg                         >  1:693174/1‑251 (MQ=255)                                                                                                                                                                                                                   aCGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAAc                                                                                                                                                         <  1:239849/110‑1 (MQ=255)                                                                                                                                                                                                                    cGCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGCAAGTAGAAACATTAGCGAGACCAATGGTAAAAAATGAATACGGTCAATACTTGCTACGTTTAATCAACGAGGAGAAATAAATGGCAAAATTTCTGATTACAGGCGCAAACGGGCAAGTGGGCTACTGTTTAAcc           <  1:451804/251‑1 (MQ=255)                                                                                                                                                                                                                     gCACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGCAAGTAGAAACATTAGCGAGACCAATGGTAAAAAATGAATACGGTCAATACTTGCTACGTTTAATCAACGAGGAGAAATAAATGGCAAAATTTCTGATTACAGGCGCAAACGGGCAAGTGGGCTACTGTTTAAcc           >  1:509187/1‑250 (MQ=255)                                                                                                                                                                                                                      cACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGCAAGTAGAAACATTAGCGAGACCAATGGTAAAAAATGAATACGGTCAATACTTGCTACGTTTAATCAACGAGGAGAAATAAATGGCAAAATTTCTGATTACAGGCGCAAACGGGCAAGTGGGCTACTGTTTAAccc          <  1:674651/250‑1 (MQ=255)                                                                                                                                                                                                                      cACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGCAAGTAGAAACATTAGCGAGACCAATGGTAAAAAATGAATACGGTCAATACTTGCTACGTTTAATCAACGAGGAGAAATAAATGGCAAAATTTCTGATTACAGGCGCAAACGGGCAAGTGGGCTACTGTTTAAccc          <  1:159541/250‑1 (MQ=255)                                                                                                                                                                                                                      cACGAAAGCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGCAAGTAGAAACATTAGCGAGACCAATGGTAAAAAATGAATACGGTCAATACTTGCTACGTTTAATCAACGAGGAGAAATAAATGGCAAAATTTCTGATTACAGGCGCAAACGGGCAAGTGGGCTACTGTTTAAccc          <  1:377791/250‑1 (MQ=255)                                                                                                                                                                                                                             gCCTACACGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGCAAGTAGAAACATTAGCGAGACCAATGGTAAAAAATGAATACGGTCAATACTTGCTACGTTTAATCAACGAGGAGAAATAAATGGCAAAATTTCTGATTACAGGCGCAAACGGGCAAGTGGGCTACTGTTTAACCCAACAATTg  >  1:314993/1‑251 (MQ=255)                                                                                                                                                                                                                                 acacGAAGGGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGCAAGTAGAAACATTAGCGAGACCAATGGTAAAAAATGAATACGGTCAATACTTGCTACGTTTAATCAACGAGGAGAAATAAATGGCAAAATTTCTGATTACAGGCGCAAACGGGCAAGTGGGCTAc                    <  1:805166/229‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               TCTATTGAAGAAAAACCTGTTCAACCTAAATCTAACTATGCTGTAACAGGTTTATATTTCTACGATAATCGTGTGGTCGATTTCGCGAAACAGGTGAAACCATCTGCGCGCGGTGAGCTTGAAATCACCACATTAAATGAAATGTATCTAAAAGATGGTTCATTAAATGTGCAATTACTCGGTCGTGGTTTTGCGTGGTTAGACACAGGAACGCACGAAAGCCTACACGAAGCGGCATCTTTCGTTCGCACTGTTGAAAGCGTACAAGGTTTGCAAGTTGCATGTTTGGAAGAAATTGCCTGGCGTAATGGTTGGCTAACTTCTGAGCAAGTAGAAACATTAGCGAGACCAATGGTAAAAAATGAATACGGTCAATACTTGCTACGTTTAATCAACGAGGAGAAATAAATGGCAAAATTTCTGATTACAGGCGCAAACGGGCAAGTGGGCTACTGTTTAACCCAACAATTG  >  1/497139‑497609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑