Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	489,319	T→C	I381V (ATC→GTC)	unknown ←	putative flippase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	489,319	0	T	C	100.0%	161.2 / NA	60	I381V (ATC→GTC)	unknown	putative flippase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/29);  total (31/29)

CGATAAATAAATTAAACCCAATAAAAAGGCAAAAATAATGCCGATATTCAGTAAGTATGAGACAAATTCTTTTCGATTAAATTTAATTTTGTTGTTAATAAAATACAGACGGTAAATTTGCATGAGTGAGGCGGTGCAGAATTTGCCGATGAACGCAAACACCGGATTGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGATGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCAATGTATGGCTGAAATACAACACCGGCGCCGATAAAATGGCGATTAAATAGAAGGAATATTTTGACGTGCCTAAAATTAACTTTTTGTTGCGATCGT  >  1/489086‑489564                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      cGATAAATAAATTAAACCCAATAAAAAGGCAAAAATAATGCCGATATTCAGTAAGTATGAGACAAATTCTTTTCGATTAAATTTAATTTTGTTGTTAATAAAATACAGACGGTAAATTTGCATGAGTGAGGCGGTGCAGAATTTGCCGATGAACGCAAACACCGGATTGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATgg                                                                                                                                                                                                                                      <  1:809327/251‑1 (MQ=255)                                         ccGATATTCAGTAAGTATGAGACAAATTCTTTTCGATTAAATTTAATTTTGTTGTTAATAAAATACAGACGGTAAATTTGCATGAGTGAGGCGGTGCAGAATTTGCCGATGAACGCAAACACCGGATTGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATcc                                                                                                                                                                                                        <  1:618158/241‑1 (MQ=255)                                                       gtaTGAGACAAATTCTTTTCGATTAAATTTAATTTTGTTGTTAATAAAATACAGACGGTAAATTTGCATGAGTGAGGCGGTGCAGAATTTGCCGATGAACGCAAACACCGGATTGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGa                                                                                                                                                                                >  1:35910/1‑251 (MQ=255)                                                              aCAAATTCTTTTCGATTAAATTTAATTTTGTTGTTAATAAAATACAGACGGTAAATTTGCATGAGTGAGGCGGTGCAGAATTTGCCGATGAACGCAAACACCGGATTGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGa                                                                                                                                                                         <  1:304035/251‑1 (MQ=255)                                                                                    taatttTGTTGTTAATAAAATACAGACGGTAAATTTGCATGAGTGAGGCGGTGCAGAATTTGCCGATGAACGCAAACACCGGATTGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCAcc                                                                                                                                                   <  1:851248/251‑1 (MQ=255)                                                                                               ttAATAAAATACAGACGGTAAATTTGCATGAGTGAGGCGGTGCAGAATTTGCCGATGAACGCAAACACCGGATTGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCgg                                                                                                                                         <  1:341006/250‑1 (MQ=255)                                                                                                  ataaAATACAGACGGTAAATTTGCATGAGTGAGGCGGTGCAGAATTTGCCGATGAACGCAAACACCGGATTGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAata                                                                                                                                     >  1:503649/1‑251 (MQ=255)                                                                                                  ataaAATACAGACGGTAAATTTGCATGAGTGAGGCGGTGCAGAATTTGCCGATGAACGCAAACACCGGATTGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAata                                                                                                                                     >  1:184515/1‑251 (MQ=255)                                                                                                  ataaAATACAGACGGTAAATTTGCATGAGTGAGGCGGTGCAGAATTTGCCGATGAACGCAAACACCGGATTGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAata                                                                                                                                     >  1:324701/1‑251 (MQ=255)                                                                                                            gACGGTAAATTTGCATGAGTGAGGCGGTGCAGAATTTGCCGATGAACGCAAACACCGGATTGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGa                                                                                                                                                                         <  1:300880/205‑1 (MQ=255)                                                                                                                gTAAATTTGCATGAGTGAGGCGGTGCAGAATTTGCCGATGAACGCAAACACCGGATTGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTAt                                                                                                                                                                                                                                                <  1:857338/130‑1 (MQ=255)                                                                                                                gTAAATTTGCATGAGTGAGGCGGTGCAGAATTTGCCGATGAACGCAAACACCGGATTGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGgcgc                                                                                                                                                                                   <  1:420566/191‑1 (MQ=255)                                                                                                                            gagtgagGCGGTGCAGAATTTGCCGATGAACGCAAACACCGGATTGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAg                                                                                                                                                 <  1:209775/213‑1 (MQ=255)                                                                                                                            gagtgagGCGGTGCAGAATTTGCCGATGAACGCAAACACCGGATTGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGc                                                                                                                                 >  1:185193/1‑229 (MQ=255)                                                                                                                            gagtgagGCGGTGCAGAATTTGCCGATGAACGCAAACACCGGATTGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGccc                                                                                                                  <  1:691018/244‑1 (MQ=255)                                                                                                                              gtgagGCGGTGCAGAATTTGCCGATGAACGCAAACACCGGATTGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAgg                                                                                                         >  1:148134/1‑251 (MQ=255)                                                                                                                               tgagGCGGTGCAGAATTTGCCGATGAACGCAAACACCGGATTGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAGCCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAggg                                                                                                        <  1:746153/251‑1 (MQ=255)                                                                                                                                     ggTGCAGAATTTGCCGATGAACGCAAACACCGGATTGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGcac                                                                                                                            >  1:675621/1‑225 (MQ=255)                                                                                                                                      gTGCAGAATTTGCCGATGAACGCAAACACCGGATTGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCaa                                                                                                 >  1:610418/1‑251 (MQ=255)                                                                                                                                           gAATTTGCCGATGAACGCAAACACCGGATTGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAggg                                                                                                        >  1:839332/1‑239 (MQ=255)                                                                                                                                              tttGCCGATGAACGCAAACACCGGATTGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCAATGTATGGc                                                                                         <  1:543404/251‑1 (MQ=255)                                                                                                                                                                 cccGGATGGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTAGCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCAATGTATGGCTGAAATACAACACCGgcgc                                                                      <  1:255014/250‑1 (MQ=255)                                                                                                                                                                    ggATTGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCAATGTATGGc                                                                                         >  1:61211/1‑229 (MQ=255)                                                                                                                                                                     gATTGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGAt                                                                                                                       >  1:54322/1‑198 (MQ=255)                                                                                                                                                                       ttGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCAATGTATGGCTGAAATACAACACCGGCGCCGATaa                                                                >  1:717390/1‑251 (MQ=255)                                                                                                                                                                       ttGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAAT‑GTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCAATGTATGGCTGAAATACAACACCGGCGCCGATaaa                                                               >  1:574095/1‑251 (MQ=255)                                                                                                                                                                         gTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAggg                                                                                                        <  1:613214/209‑1 (MQ=255)                                                                                                                                                                           aGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCAATGTATGGCTGAAATac                                                                                 <  1:834028/230‑1 (MQ=255)                                                                                                                                                                              ccAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCAATGTATGGCTGAAATACAACACCGGCGCCGATAAAATGGCg                                                         <  1:171358/251‑1 (MQ=255)                                                                                                                                                                                   gtgAATAAATAATAGGCGAGCGGTTCGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGa                                                                                                                                                                         <  1:162901/134‑1 (MQ=255)                                                                                                                                                                                     gAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCAATGTATGGCTGAAATACAACACCGGCGCCGATAAAATGGCGATTAAAt                                                  >  1:453203/1‑251 (MQ=255)                                                                                                                                                                                       ataaataaTAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCAATGTATGGCTGAAATACAACACCGGCGCCGATAAAATGGCGATTAAATAg                                                >  1:649802/1‑251 (MQ=255)                                                                                                                                                                                          aataatAGGCGAGCGGCACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAggg                                                                                                        >  1:191556/1‑192 (MQ=255)                                                                                                                                                                                           ataataGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGAt                                                                                                                       >  1:355063/1‑176 (MQ=255)                                                                                                                                                                                           ataataGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCAATGTATGGCTGAAATACAACACCGGCGCCGATAAAATGGCGATTAAATAGAAgg                                            >  1:237315/1‑251 (MQ=255)                                                                                                                                                                                                  gCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCAATGTATGGCTGAAATACAACACCGGCGCCGATAAAATGGCGATTAAATAGAAGGAatat                                       <  1:56133/249‑1 (MQ=255)                                                                                                                                                                                                    gAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGc                                                                                                   >  1:443960/1‑187 (MQ=255)                                                                                                                                                                                                      gCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCAATGTATGGCTGAAATACAACACCGGCGCCGATAAAATGGCGATTAAATAGAAg                                             <  1:609699/239‑1 (MQ=255)                                                                                                                                                                                                      gCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCAATGTATGGCTGAAATACAACACCGGCGCCGATAAAATGGCGATTAAATAGAAGGAATATTTTGa                                  >  1:511455/1‑250 (MQ=255)                                                                                                                                                                                                         gTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAggg                                                                                                        >  1:532714/1‑177 (MQ=255)                                                                                                                                                                                                         gTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAggg                                                                                                        <  1:351479/177‑1 (MQ=255)                                                                                                                                                                                                         gTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCAATGTATGGCTGAAATACAACACCGGCGCCGATAAAATGGCGATTAAATAGAAGGAATATTTTGACGTg                              >  1:601138/1‑251 (MQ=255)                                                                                                                                                                                                         gTACGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCAATGTATGGCCGAAATACAACACCGGCGCCGATAAAATGGCGATTAAATAGAAGGAATATTTTGACGTg                              >  1:539946/1‑251 (MQ=255)                                                                                                                                                                                                           aCGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATgg                                                                                                                                                                                                                                      >  1:393241/1‑49 (MQ=255)                                                                                                                                                                                                           aCGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGTAGGAAcc                                                                                                                                                                                              >  1:186523/1‑89 (MQ=255)                                                                                                                                                                                                           aCGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCAATGTATGGCTGAAATacaac                                                                              <  1:2670/201‑1 (MQ=255)                                                                                                                                                                                                           aCGGTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCAATGTATGGCTGAAATACAACACCGGCGCCGATAAAATGGCGATTAAATAGAAGGAATATTTTGACGTGc                             >  1:74542/1‑250 (MQ=255)                                                                                                                                                                                                              gTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAgg                                                                                                                                                                          <  1:278821/106‑1 (MQ=255)                                                                                                                                                                                                              gTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCaa                                                                                                 <  1:411271/179‑1 (MQ=255)                                                                                                                                                                                                              gTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCAATGTATGGCTGAAATACAACACCGGCGCCGATAAAATGGCGATTAAATAGAAGGAATATTTTGACGTGCCTaa                         >  1:99572/1‑251 (MQ=255)                                                                                                                                                                                                              gTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCAATGTATGGCTGAAATACAACACCGGCGCCGATAAAATGGCGATTAAATAGAAGGAATATTTTGACGTGCCTaa                         >  1:828759/1‑251 (MQ=255)                                                                                                                                                                                                              gTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCAATGTATGGCTGAAATACAACACCGGCGCCGATAAAATGGCGATTAAATAGAAGGAATATTTTGACGTGCCTaa                         >  1:294602/1‑251 (MQ=255)                                                                                                                                                                                                              gTGCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGAGGCACATGATCGCCCAGCCAAAGGGTGAGCAATGTATGGCTGAAATACAACACCGGCGCCGATAAAATGGCGATTAAATAGAAGGAATATTTTGACGTGCCTaa                         >  1:803120/1‑251 (MQ=255)                                                                                                                                                                                                                gCACAGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAatat                                                                                                                                    <  1:64708/142‑1 (MQ=255)                                                                                                                                                                                                                 cacaGGTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCAATGTATGGCTGAAATACAACACCGGCGCCGATAAAATGGCGATTAAATAGAAGGAATATTTTGACGTGCCTAAAAt                      <  1:586913/251‑1 (MQ=255)                                                                                                                                                                                                                     ggTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAg                                                                                                                                                 >  1:676107/1‑124 (MQ=255)                                                                                                                                                                                                                     ggTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAggg                                                                                                        <  1:88702/165‑1 (MQ=255)                                                                                                                                                                                                                     ggTCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCAATGTATGGCTGAAATACAACACCGGCGCCGATAAAATgg                                                           <  1:816153/210‑1 (MQ=255)                                                                                                                                                                                                                       tCAATGGTTGCCAAAACGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCAATGTATGGCTGAAATACAACACCGGCGCCGATAAAATGGCGATTAAATAGAAGGAATATTTTGACGTGCCTAAAATTAACt                 <  1:354152/250‑1 (MQ=255)                                                                                                                                                                                                                                     aaCGACGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCAATGTATGGCTGAAATACAACACCGGCGCCGATAAAATGGCGATTAAATAGAAGGAATATTTTGACGTGCCTAAAATTAACTTTTTGTTGCGATCGt  <  1:63795/251‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      CGATAAATAAATTAAACCCAATAAAAAGGCAAAAATAATGCCGATATTCAGTAAGTATGAGACAAATTCTTTTCGATTAAATTTAATTTTGTTGTTAATAAAATACAGACGGTAAATTTGCATGAGTGAGGCGGTGCAGAATTTGCCGATGAACGCAAACACCGGATTGTAGCCCAGTGTGAATAAATAATAGGCGAGCGGTACGGTGCACAGGTCAATGGTTGCCAAAACGATGTTGTATCGTTTGATGGTGTGCGAACCGATGGCAAGCGCCGTCATCCAGAAGGAACCCGCCATGGCGCTGATTAAGGAACAAACAATGGTGGCTTGCACCAGTTTATCGGAATATTGCGGCACATGATCGCCCAGCCAAAGGGTGAGCAATGTATGGCTGAAATACAACACCGGCGCCGATAAAATGGCGATTAAATAGAAGGAATATTTTGACGTGCCTAAAATTAACTTTTTGTTGCGATCGT  >  1/489086‑489564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑