Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	484,887	T→C	V528A (GTG→GCG)	unknown →	ATP‑dependent DNA helicase UvrD/PcrA (EC 3.6.4.12)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	484,887	0	T	C	100.0%	192.6 / NA	71	V528A (GTG→GCG)	unknown	ATP‑dependent DNA helicase UvrD/PcrA (EC 3.6.4.12)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/39);  total (32/39)

GAAAACAAACTCGCCGGGCGCGCCGCTACGGCATTGTTACGCTTTATGGAGTTAATTAATTCTTTGCAGCGGGACACGGAAGAAATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTACGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGTGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTCGAAATGATGACGCTGCATTCGGTAAAAGGCTTGGAGTTTCCGCGTGTTTTCATGATTGGCGTGGAAGAAGGATTATTCCCGAGTTTCCGTTCTTTTGAAGAAGCGGGGCGTCTGGAAGAAGAGCGCCGTTTGGCATACGTCGGGATTAC  >  1/484652‑485124                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                              gAAAACAAACTCGCCTGGCGCGCTGCTACGGCATTGTTACGCTTTATGGAGTTAATTAATTCTTTGCAGCGGGACACGGAAGAAATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGAc                                                                                                                                                                                                                                   <  1:543343/248‑1 (MQ=255) gAAAACAAACTCGCCTGGCGCGCTGCTACGGCATTGTTACGCTTTATGGAGTTAATTAATTCTTTGCAGCGGGACACGGAAGAAATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGAc                                                                                                                                                                                                                                   <  1:806905/248‑1 (MQ=255) gAAAACAAACTCGCCTGGCGCGCTGCTACGGCATTGTTACGCTTTATGGAGTTAATTAATTCTTTGCAGCGGGACACGGAAGAAATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACgg                                                                                                                                                                                                                                 <  1:482220/250‑1 (MQ=255)  aaaaCAAACTCGCCTGGCGCGCTGCTACGGCATTGTTACGCTTTATGGAGTTAATTAATTCTTTGCAGCGGGACACGGAAGAAATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGa                                                                                                                                                                                                                                <  1:709317/250‑1 (MQ=255)   aaacaaacTCGCCTGGCGCGCTGCTACGGCATTGTTACGCTTTATGGAGTTAATTAATTCTTTGCAGCGGGACACGGAAGAAATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATc                                                                                                                                                                                                                              <  1:770728/251‑1 (MQ=255)        aacTCGCCTGGCGCGCTGCTACGGCATTGTTACGCTTTATGGAGTTAATTAATTCTTTGCAGCGGGACACGGAAGAAATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACg                                                                                                                                                                                                                         <  1:556795/251‑1 (MQ=255)                     cgctgcTACGGCATTGTTACGCTTTATGGAGTTAATTAATTCTTTGCAGCGGGACACGGAAGAAATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACt                                                                                                                                                                                                             <  1:403211/246‑1 (MQ=255)                     cgctgcTACGGCATTGTTACGCTTTATGGAGTTAATTAATTCTTTGCAGCGGGACACGGAAGAAATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTc                                                                                                                                                                                                            <  1:755563/247‑1 (MQ=255)                       ctgcTACGGCATTGTTACGCTTTATGGAGTTAATTAATTCTTTGCAGCGGGACACGGAAGAAATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCa                                                                                                                                                                                                           >  1:531213/3‑250 (MQ=255)                       ctgcTACGGCATTGTTACGCTTTATGGAGTTAATTAATTCTTTGCAGCGGGACACGGAAGAAATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCa                                                                                                                                                                                                           >  1:331277/3‑250 (MQ=255)                                 aTTGTTACGCTTTATGGAGTTAATTAATTCTTTGCAGCGGGACACGGAAGAAATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTgg                                                                                                                                                                                                <  1:371483/251‑1 (MQ=255)                                     ttACGCTTTATGGAGTTAATTAATTCTTTGCAGCGGGACACGGAAGAAATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAgg                                                                                                                                                                                             <  1:156473/250‑1 (MQ=255)                                        cGCTTTATGGGGTTAATTAATTCTTTGCAGCGGGACACGGAAGAAATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAggcggg                                                                                                                                                                                         >  1:892089/1‑251 (MQ=255)                                         gCTTTATGGAGTTAATTAATTCTTTGCAGCGGGACACGGAAGAAATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGAc                                                                                                                                                                                                                                   <  1:74681/208‑1 (MQ=255)                                          cTTTATGGAGTTAATTAATTCTTTGCAGCGGGACACGGAAGAAATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAGACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAggcgggcg                                                                                                                                                                                       >  1:376517/1‑251 (MQ=255)                                          cTTTATGGAGTTAATTAATTCTTTGCAGCGGGACACGGAAGAAATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAggcgggcg                                                                                                                                                                                       >  1:899684/1‑251 (MQ=255)                                            ttATGGAGTTAATTAATTCTTTGCAGCGGGACACGGAAGAAATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCgag                                                                                                                                                                                     >  1:410702/1‑251 (MQ=255)                                                      aattaattCTTTGCAGCGGGACACGGAAGAAATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAgg                                                                                                                                                                              >  1:119144/1‑248 (MQ=255)                                                          aattCTTTGCAGCGGGACACGGAAGAAATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGcc                                                                                                                                                                       <  1:360832/251‑1 (MQ=255)                                                             tCTTTGCAGCGGGACACGGAAGAAATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTa                                                                                                                                                                                                                >  1:541663/1‑207 (MQ=255)                                                               tttGCAGCGGGACACGGAAGAAATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATc                                                                                                                                                                  <  1:28706/251‑1 (MQ=255)                                                                      cGGGACACGGAAGAAATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGc                                                                                                                                                              <  1:224412/248‑1 (MQ=255)                                                                      cGGGACACGGAAGAAATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCt                                                                                                                                                             <  1:780508/249‑1 (MQ=255)                                                                      cGGGACACGGAAGAAATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCt                                                                                                                                                             <  1:465670/249‑1 (MQ=255)                                                                          acacGGAAGAAATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGc                                                                                                                                                                             <  1:863444/229‑1 (MQ=255)                                                                               gaagaaATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGaaa                                                                                                                                                   <  1:235853/250‑1 (MQ=255)                                                                               gaagaaATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGaaa                                                                                                                                                   <  1:792460/250‑1 (MQ=255)                                                                               gaagaaATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAat                                                                                                                                                  >  1:281614/1‑251 (MQ=255)                                                                                                  cgcAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGTCGGATCTGGCGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGATGCTGCATTCGGt                                                                                                                                <  1:78286/250‑1 (MQ=255)                                                                                                  cgcAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGt                                                                                                                                <  1:660590/250‑1 (MQ=255)                                                                                                      aaCGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTgg                                                                                                                                                                                                >  1:477425/1‑182 (MQ=255)                                                                                                        cGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGc                                                                                                                         <  1:539257/251‑1 (MQ=255)                                                                                                          gATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGCtt                                                                                                                       >  1:314693/1‑251 (MQ=255)                                                                                                            ttttGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGc                                                                                                                         >  1:121768/1‑247 (MQ=255)                                                                                                              ttGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGCTTGGAg                                                                                                                   <  1:21712/251‑1 (MQ=255)                                                                                                              ttGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGCTTGGAg                                                                                                                   <  1:702108/251‑1 (MQ=255)                                                                                                                 tgATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGCTTGGAGttt                                                                                                                >  1:730571/1‑251 (MQ=255)                                                                                                                     tAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGt                                                                                                                                <  1:577582/231‑1 (MQ=255)                                                                                                                          aTTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGCTTGGAGTTTCCGCGTGtt                                                                                                       >  1:769372/1‑251 (MQ=255)                                                                                                                           ttCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAggc                                                                                                                                                                                            <  1:767417/165‑1 (MQ=255)                                                                                                                               gggTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGAc                                                                                                                                                                                                                                   >  1:871816/1‑122 (MQ=255)                                                                                                                               gggTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGc                                                                                                                         <  1:622001/228‑1 (MQ=255)                                                                                                                               gggTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGCTTGGAGTTTCCGCGTGTTTTCAt                                                                                                  >  1:792026/1‑251 (MQ=255)                                                                                                                                    gTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAgg                                                                                                                                                                                             >  1:169768/1‑155 (MQ=255)                                                                                                                                    gTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGc                                                                                                                                          >  1:699673/1‑206 (MQ=255)                                                                                                                                    gTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGc                                                                                                                         <  1:632251/223‑1 (MQ=255)                                                                                                                                    gTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGCTTGGAGTTTCCGCGTGTTTTCATGATTg                                                                                             >  1:853839/1‑251 (MQ=255)                                                                                                                                        cAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGCTTGGAGTTTCCGCGTGTTTTCATGATTGGCgg                                                                                         >  1:173154/1‑250 (MQ=255)                                                                                                                                          aaTGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGAc                                                                                                                                                                                                                                   <  1:617908/111‑1 (MQ=255)                                                                                                                                             gTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAgg                                                                                                                                                                                             >  1:870549/1‑146 (MQ=255)                                                                                                                                                  aaCAGGAAAAGGGCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGCTTGGAGTTTCCGCGTGTTTTCATGATTGGCGTGGAAGAAGGa                                                                               >  1:848241/1‑251 (MQ=255)                                                                                                                                                            gggCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGCTTGGAGTTTCCGCGTGTTTTCATGATTGGCGTGGAAGAAGGATTATTCCCGa                                                                     >  1:753392/1‑251 (MQ=255)                                                                                                                                                            gggCGAAAAAGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGCTTGGAGTTTCCGCGTGTTTTCATGATTGGCGTGGAAGAAGGATTATTCCCGa                                                                     >  1:577202/1‑251 (MQ=255)                                                                                                                                                                    aaGGGGAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGCTTGGAGTTTCCGCGTGTTTTCATGATTGGCGTGGAAGAAGGATTATTCCCGAGTTTCCGt                                                             >  1:344721/1‑251 (MQ=255)                                                                                                                                                                      ggggAGGTGCGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGACGACGCTGCATTCGGTAAAAGGCTTGGAGTTTCCGCGTGTTTTCATGATTGGCGTGGAAGAAGGATTATTCCCGAGTTTCCGtt                                                            >  1:194663/1‑250 (MQ=255)                                                                                                                                                                              gcGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCt                                                                                                                                                             <  1:306674/144‑1 (MQ=255)                                                                                                                                                                               cGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATCTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGCTTGGAGTTTCCGCGTGTTTTCATGATTGGCGTGGAAGAAGGATTATTCCCGAGTTTCCGTTCTTTTgaag                                                   <  1:232659/250‑1 (MQ=255)                                                                                                                                                                                   ttGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGCTTGGAGTTTCCGCGTGTTTTCATGATTGGCGTGGAAGAAGGATTATTCCCg                                                                      <  1:396035/227‑1 (MQ=255)                                                                                                                                                                                         aCCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGCTTGGAGTTTCCGCGTGTTTTCATGATTGGCGTGGAAGAAGGATTATTCCCGAGTTTCCGTTCTTTTGAAGAAGCGGGGCGt                                        >  1:571353/1‑251 (MQ=255)                                                                                                                                                                                                 gaaTTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGCTTGGAGTTTCCGCGTGTTTTCATGATTGGCGTGGAAGAAGGa                                                                               <  1:388727/204‑1 (MQ=255)                                                                                                                                                                                                 gaaTTGGTGTCAGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGCTTGGAGTTTCCGCGTGTTTTCATGATTGGCGTGgaa                                                                                     >  1:235705/1‑198 (MQ=255)                                                                                                                                                                                                     tGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCGCGCTTTATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGCTTGGAGTTTCCGCGTGTTTTCATGATTGGCGTGGAAGAAGGATTATTCCCGAGTTTCCGTTCTTTTGAAGAAGCGGGGCGTCTGGAAGAagag                            <  1:434712/251‑1 (MQ=255)                                                                                                                                                                                                     tGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGCTTGGAGTTTCCGCGTGTTTTCATGATTGGCGTGGAAGAAGGATTATTCCCGAGTTTCCGTTCTTTTGAAGAAGCGGGGCGTCTGGAAGAagag                            <  1:227467/251‑1 (MQ=255)                                                                                                                                                                                                      ggTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGCTTGGAGTTTCCGCGTGTTTTCATGATTGGCGTGGAAGAAGGATTATTCCCGAGTTTCCGTTCTTTTGAAGAAGCGGGGCGTCTGGAAGAagag                            >  1:852175/1‑250 (MQ=255)                                                                                                                                                                                                                   cGTGAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGCTTGGAGTTTCCGCGTGTTTTCATGATTGGCGTGGAAGAAGGATTATTCCCGAGTTTCCGTTCTTTTGAAGAAGCGGGGCGTCTGGAAGAAGAGCGCCGTTTGGCATa              <  1:532592/251‑1 (MQ=255)                                                                                                                                                                                                                      gAATTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGCTTGGAGTTTCCGCGTGTTTTCATGATTGGCGTGGAAGAAGGATTATTCCCGAGTTTCCGTTCTTTTGAAGAAGCGGGGCGTCTGGAAGAAGAGCGCCGTTTGGCATACGt           >  1:894966/1‑251 (MQ=255)                                                                                                                                                                                                                       aaTTTATCAAACCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGCTTGGAGTTTCCGCGTGTTTTCATGATTGGCGTGGAAGAAGGATTATTCCCGAGTTTCCGTTCTTTTGAAGAAGCGGGGCGTCTGGAAGAAGAGCGCCGTTTGGCATACGt           <  1:20983/250‑1 (MQ=255)                                                                                                                                                                                                                                aaCCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGCTTGGAGTTTCCGCGTGTTTTCATGAtt                                                                                              <  1:703286/158‑1 (MQ=255)                                                                                                                                                                                                                                aaCCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGCTTGGAGTTTCCGCGTGTTTTCATGATTGGCGTGGAAGAAGGATTATTCCCGAGTTTCCGTTCTTTTGAAGAAGCGGGGCGTCTGGAAGAAGAGCGCCGTTTGGCATACGTCGGGATTAc  >  1:95355/1‑250 (MQ=255)                                                                                                                                                                                                                                aaCCTGAAGACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGACGCTGCATTCGGTAAAAGGCTTGGAGTTTCCGCGTGTTTTCATGATTGGCGTGGAAGAAGGATTATTCCCGAGTTTCCGTTCTTTTGAAGAAGCGGGGCGTCTGGAAGAAGAGCGCCGTTTGGCATACGTCGGGATTAc  >  1:880498/1‑250 (MQ=255)                                                                                                                                                                                                                                        gACGCGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTTGAAATGATGAc                                                                                                                                            <  1:502665/104‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                              GAAAACAAACTCGCCGGGCGCGCCGCTACGGCATTGTTACGCTTTATGGAGTTAATTAATTCTTTGCAGCGGGACACGGAAGAAATGCCGTTGTTTGCGCAAACGGATTTTGTGATTAAGCATTCCGGGTTGTATCAAATGTACAAACAGGAAAAGGGCGAAAAAGGGGAGGTACGTATTGAAAACCTGGAAGAATTGGTGTCTGCCGCCCGTGAATTTATCAAACCTGAAGACGTGGAAGATATGACGGATCTGACGGCATTTTTAACTCACGCTTCATTGGAGGCGGGCGAGGAGCAGGCTGCGCCACATCAGGCTTGCGTCGAAATGATGACGCTGCATTCGGTAAAAGGCTTGGAGTTTCCGCGTGTTTTCATGATTGGCGTGGAAGAAGGATTATTCCCGAGTTTCCGTTCTTTTGAAGAAGCGGGGCGTCTGGAAGAAGAGCGCCGTTTGGCATACGTCGGGATTAC  >  1/484652‑485124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑