Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	483,847	G→A	Q181Q (CAG→CAA)	unknown →	ATP‑dependent DNA helicase UvrD/PcrA (EC 3.6.4.12)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	483,847	0	G	A	100.0%	202.9 / NA	74	Q181Q (CAG→CAA)	unknown	ATP‑dependent DNA helicase UvrD/PcrA (EC 3.6.4.12)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/40);  total (34/40)

ATTGCTACGCGCCCATCACCTTGATGCGGATCTGCCGCAAGATTTCCAGATTTTGGATTCGGAAGATCAGTTGCGTTTAATCAAACGCTTAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAGGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAATTCAATATGAATGGATTCGCTTGCTGGCGGGCAAAACCGGCAAAGTGATGATTGTGGGCGATGACGACCAGTCTATTTACGGC  >  1/483601‑484078                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        aTTGCTACGCGCCCATCACCTTGATGCGGATCTGCCGCAAGATTTCCAGATTTTGGATTCGGAAGATCAGTTGCGTTTAATCAAACGCTTAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATg                                                                                                                                                                                                                                     <  1:2166/251‑1 (MQ=255)     cTACGCGCCCATCACCTTGATGCGGATCTGCCGCAAGATTTCCAGATTTTGGATTCGGAAGATCAGTTGCGTTTAATCAAACGCTTAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTg                                                                                                                                                                                                                                 <  1:838981/251‑1 (MQ=255)          cgcCCATCACCTTGATGCGGATCTGCCGCAAGATTTCCAGATTTTGGATTCGGAAGATCAGTTGCGTTTAATCAAACGCTTAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGAt                                                                                                                                                                                                                             <  1:67798/250‑1 (MQ=255)          cgcCCATCACCTTGATGCGGATCTGCCGCAAGATTTCCAGATTTTGGATTCGGAAGATCAGTTGCGTTTAATCAAACGCTTAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGAt                                                                                                                                                                                                                             <  1:280179/250‑1 (MQ=255)            cccATCACCTTGATGCGGATCTGCCGCAAGATTTCCAGATTTTGGATTCGGAAGATCAGTTGCGTTTAATCAAACGCTTAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGt                                                                                                                                                                                                                          >  1:180755/1‑251 (MQ=255)              cATCACCTTGATGCGGATCTGCCGCAAGATTTCCAGATTTTGGATTCGGAAGATCAGTTGCGTTTAATCAAACGCTTAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTg                                                                                                                                                                                                                         >  1:858436/1‑250 (MQ=255)              cATCACCTTGATGCGGATCTGCCGCAAGATTTCCAGATTTTGGATTCGGAAGATCAGTTGCGTTTAATCAAACGCTTAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGc                                                                                                                                                                                                                        <  1:895530/251‑1 (MQ=255)                  aCCTTGATGCGGATCTGCCGCAAGATTTCCAGATTTTGGATTCGGAAGATCAGTTGCGTTTAATCAAACGCTTAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAggg                                                                                                                                                                                                                    <  1:489617/251‑1 (MQ=255)                   ccTTGATGCGGATCTGCCGCAAGATTTCCAGATTTTGGATTCGGAAGATCAGTTGCGTTTAATCAAACGCTTAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGt                                                                                                                                                                                                                   <  1:789877/251‑1 (MQ=255)                   ccTTGATGCGGATCTGCCGCAAGATTTCCAGATTTTGGATTCGGAAGATCAGTTGCGTTTAATCAAACGCTTAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGt                                                                                                                                                                                                                   <  1:225901/251‑1 (MQ=255)                       gATGCGGATCTGCCGCAAGATTTCCAGATTTTGGATTCGGAAGATCAGTTGCGTTTAATCAAACGCTTAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTtggt                                                                                                                                                                                                               >  1:604028/1‑251 (MQ=255)                       gATGCGGATCTGCCGCAAGATTTCCAGATTTTGGATTCGGAAGATCAGTTGCGTTTAATCAAACGCTTAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTtggt                                                                                                                                                                                                               >  1:813899/1‑251 (MQ=255)                        aTGCGGATCTGCCGCAAGATTTCCAGATTTTGGATTCGGAAGATCAGTTGCGTTTAATCAAACGCTTAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATg                                                                                                                                                                                                                                     <  1:758472/228‑1 (MQ=255)                             gATCTGCCGCAAGATTTCCAGATTTTGGATTCGGAAGATCAGTTGCGTTTAATCAAACGCTTAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCCCAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGAttt                                                                                                                                                                                                         >  1:430373/1‑251 (MQ=255)                                  gCCGCAAGATTTCCAGATTTTGGATTCGGAAGATCAGTTGCGTTTAATCAAACGCTTAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGt                                                                                                                                                                                                                   >  1:777913/1‑236 (MQ=255)                                        aGATTTCCAGATTTTGGATTCGGAAGATCAGTTGCGTTTAATCAAACGCTTAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAggg                                                                                                                                                                                                                    >  1:28343/1‑229 (MQ=255)                                             tCCAGATTTCGGATTCGGAAGATCAGTTGCGTTTAATCAAACGCTTAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTttatta                                                                                                                                                                                         <  1:629586/251‑1 (MQ=255)                                                      tGGATTCGGAAGATCAGTTGCGTTTAATCAAACGCTTAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTAc                                                                                                                                                                                <  1:202280/251‑1 (MQ=255)                                                        gATTCGGAAGATCAGTTGCGTTTAATCAAACGCTTAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACg                                                                                                                                                                               <  1:312756/250‑1 (MQ=255)                                                              gAAGATCAGTTGCGTTTAATCAAACGCTTAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGtt                                                                                                                                                                        >  1:763276/1‑251 (MQ=255)                                                                   tCAGTTGCGTTTAATCAAACGCTTAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTa                                                                                                                                                                    >  1:652447/1‑250 (MQ=255)                                                                       ttGCGTTTAATCAAACGCTTAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTacac                                                                                                                                                                 <  1:59417/249‑1 (MQ=255)                                                                                aTCAAACGCTTAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTac                                                                                                                                                                   <  1:232767/238‑1 (MQ=255)                                                                                      cGCTTAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCa                                                                                                                                                >  1:739441/1‑251 (MQ=255)                                                                                          tAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGt                                                                                                                                            >  1:394166/1‑251 (MQ=255)                                                                                          tAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTCGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGt                                                                                                                                            >  1:28633/1‑251 (MQ=255)                                                                                                 aCTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATcaac                                                                                                                                     <  1:36817/251‑1 (MQ=255)                                                                                                     cacaATTTCGACG‑AAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACg                                                                                                                                 <  1:286214/250‑1 (MQ=255)                                                                                                                                         gCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGa                                                                                                                                                       <  1:288568/193‑1 (MQ=255)                                                                                                                                           cTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAg                                                                                                                          >  1:109537/1‑220 (MQ=255)                                                                                                                                             gTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGAt                                                                                               >  1:542337/1‑245 (MQ=255)                                                                                                                                             gTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACcaa                                                                                          >  1:478598/1‑250 (MQ=255)                                                                                                                                               tGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGAGTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGa                                                                                                <  1:652028/242‑1 (MQ=255)                                                                                                                                                 gTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATc                                                                                                                                        <  1:85863/200‑1 (MQ=255)                                                                                                                                                    cATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAAtt                                                                                   >  1:218697/1‑250 (MQ=255)                                                                                                                                                    cATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAAtt                                                                                   >  1:144801/1‑250 (MQ=255)                                                                                                                                                      tCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATTAAGATGCCTGCGACCGTGCAGGGTTGGAGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGc                                                                                                                         >  1:179428/1‑210 (MQ=255)                                                                                                                                                          taataaGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATcaac                                                                                                                                     >  1:680820/1‑194 (MQ=255)                                                                                                                                                           aataaGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAATTCAATATGa                                                                           >  1:336616/1‑251 (MQ=255)                                                                                                                                                                gaaagaTGAAGGGCTGCGTCCGAATCACATTGACGATCTCAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTtggtg                                                                                                                                                                                                              <  1:69359/115‑1 (MQ=255)                                                                                                                                                                  aagaTGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTGCGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAATTCAATATGAATGGAtt                                                                    <  1:172719/251‑1 (MQ=255)                                                                                                                                                                   agaTGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAATTCAATATGAATGGAtt                                                                    >  1:725270/1‑250 (MQ=255)                                                                                                                                                                    gaTGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGtt                                                                                                      <  1:3470/215‑1 (MQ=255)                                                                                                                                                                    gaTGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAATTc                                                                                  >  1:481207/1‑235 (MQ=255)                                                                                                                                                                     aTGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAg                                                                                                 <  1:679655/219‑1 (MQ=255)                                                                                                                                                                       gAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAATTCAATATGAATGGATTCGCtt                                                               >  1:534263/1‑251 (MQ=255)                                                                                                                                                                           ggCTGCGTCCTAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAATTCAatat                                                                             <  1:235841/233‑1 (MQ=255)                                                                                                                                                                             cTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCTTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAATTCAATATGAATGGATTCGCTTGCTgg                                                          <  1:446238/250‑1 (MQ=255)                                                                                                                                                                               gCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAATTCAATATGAATGGATTCGCTTGCTGGCgg                                                       >  1:12166/1‑251 (MQ=255)                                                                                                                                                                                 gTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAAt                                                                                    >  1:555483/1‑220 (MQ=255)                                                                                                                                                                                          acaTTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACcaac                                                                                         <  1:337235/206‑1 (MQ=255)                                                                                                                                                                                          acaTTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAATTCAATATGAATGGATTCGCTTGCTGGCGGGCAAAACCgg                                             >  1:850305/1‑250 (MQ=255)                                                                                                                                                                                           caTTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTacac                                                                                                                                                                 <  1:482436/133‑1 (MQ=255)                                                                                                                                                                                                aCGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCATCAAAATTCAATATGAATGGATTCGCTTGCTGGCGGGCAAAACCGGCAAAGt                                       <  1:864374/250‑1 (MQ=255)                                                                                                                                                                                                   aTCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATcaac                                                                                                                                     <  1:287729/153‑1 (MQ=255)                                                                                                                                                                                                    tCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGAt                                                                                               <  1:540406/190‑1 (MQ=255)                                                                                                                                                                                                     cacaACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAATTCAATATGAATGGATTCGCTTGCTGGCGGGCAAAACCGGCAAAGTgatgat                                 <  1:754991/251‑1 (MQ=255)                                                                                                                                                                                                      acaACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAATTCAATATGAATGGATTCGCTTGCTGGCGGGCAAAACCGGCAAAGTGATGAtt                                <  1:622200/251‑1 (MQ=255)                                                                                                                                                                                                       caACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAATTCAATATGAATGGATTCGCTTGCTGGCGGTCAAAACCGGCAAAGTGATGATtg                               >  1:773167/1‑251 (MQ=255)                                                                                                                                                                                                       caACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAATTCAATATGAATGGATTCGCTTGCTGGCGGGCAAAACCGGCAAAGTGATGATtg                               <  1:551920/251‑1 (MQ=255)                                                                                                                                                                                                       caACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAATTCAATATGAATGGATTCGCTTGCTGGCGGGCAAAACCGGCAAAGTGATGATtg                               <  1:298061/251‑1 (MQ=255)                                                                                                                                                                                                       caACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAATTCAATATGAATGGATTCGCTTGCTGGCGGGCAAAACCGGCAAAGTGATGATtg                               <  1:343983/251‑1 (MQ=255)                                                                                                                                                                                                                    gAACGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCGAGATACCAACAAAATTCAATATGAATGGATTCGCTTGCTGGCGGGCAAAACCGGCAAAGTGATGATTGTGGGCGATgacga                  >  1:433823/1‑251 (MQ=255)                                                                                                                                                                                                                     aaCGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAATTCAATATGAATGGATTCGCTTGCTgg                                                          >  1:26812/1‑210 (MQ=255)                                                                                                                                                                                                                     aaCGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAATTCAATATGAATGGATTCGCTTGCTGGCGGGCAAAACCGGCAAAg                                        >  1:286230/1‑228 (MQ=255)                                                                                                                                                                                                                       cGTGAATGGATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAATTCAATATGAATGGATTCGCTTGCTGGCGGGCAAAACCGGCAAAGTgatgat                                 >  1:526776/1‑233 (MQ=255)                                                                                                                                                                                                                               gATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATcaac                                                                                                                                     >  1:547209/1‑125 (MQ=255)                                                                                                                                                                                                                               gATCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAATTCAATATGAATGGATTCGCTTGCTGGCGGGCAAAACCGGCAAAGTGATGATTGTGGGCGATGACGACCAGTCTAttt       >  1:67797/1‑251 (MQ=255)                                                                                                                                                                                                                                aTCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAATTCAATATGAATGGATTCGCTTGCTGGCGGGCAAAACCGGCAAAGTGATGATTGTGGGCGATGACGACCAGTCTATTTa      <  1:866781/251‑1 (MQ=255)                                                                                                                                                                                                                                 tCAAAATTTATCAAATTTATCAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAATTCAATATGAATGGATTCGCTTGCTGGCGGGCAAAACCGGCAAAGTGATGATTGTGGGCGATGACGACCAGTCTATTTAc     <  1:439686/251‑1 (MQ=255)                                                                                                                                                                                                                                    aaatttatcaaatttatcAAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAATTCAATATGAATGGATTCGCTTGCTGGCGGGCAAAACCGGCAAAGTGATGATTGTGGGCGATGACGACCAGTCTATTTACGGc  <  1:580216/251‑1 (MQ=255)                                                                                                                                                                                                                                                tttatcaAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAATTCAATATGAATGGATTCGCTTGCTGGCGGGCAAAACCGGCaa                                          <  1:157192/199‑1 (MQ=255)                                                                                                                                                                                                                                                   atcaAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATcaac                                                                                                                                     >  1:423882/1‑105 (MQ=255)                                                                                                                                                                                                                                                   atcaAGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAATTCAatat                                                                             <  1:518779/161‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        ATTGCTACGCGCCCATCACCTTGATGCGGATCTGCCGCAAGATTTCCAGATTTTGGATTCGGAAGATCAGTTGCGTTTAATCAAACGCTTAATGAAACTGCACAATTTCGACGAAAAAAGTTTTCCACCGAAACAAGCCTGTTGGTACATCAATAATAAGAAAGATGAAGGGCTGCGTCCGAATCACATTGACGATCACAACGATCGTCAGGAACGTGAATGGATCAAAATTTATCAAATTTATCAGGATGCCTGCGATCGTGCAGGGTTGGTGGATTTTGCCGAAATTTTATTACGTTCTTACGAACTGTTTTTACACAAACCGCTGATTTTGCAACGTTATCAACAACGTTTTCAGCATATTTTGGTGGATGAGTTCCAAGATACCAACAAAATTCAATATGAATGGATTCGCTTGCTGGCGGGCAAAACCGGCAAAGTGATGATTGTGGGCGATGACGACCAGTCTATTTACGGC  >  1/483601‑484078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑