Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 482,652 A→G S291G (AGT→GGT)  unknown → Low‑affinity gluconate/H+ symporter GntU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1482,6520AG100.0% 173.3 / NA 64S291G (AGT→GGT) unknownLow‑affinity gluconate/H+ symporter GntU
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/30);  total (34/30)

AACACCGCCGCCATCAGCGAAACCTCCGTGCAAAATCCACCGGCGTTTGGGCGCGTTCTGTTTATTTTGGTTTTACCGATTTTCCTTATTTTGTTTGATACCGGGTTAAATACCCTCAGCGTGGCAAAAGTGATTGACGGTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCAGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGTATGCCGATCATCATCACCGCCTTTATTATTTCTATGGCATTGCGTGTGGCACAAGGTTCGGCAACGGTAGCCCTGACTACTGC  >  1/482412‑482884
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                        
aaCACCGCCGCCATCAGCGAAACCTCCGTGCAAAATCCACCGGCGTTTGGGCGCGTTCTGTTTATTTTGGTTTTACCGATTTTCCTTATTTTGTTTGATACCGGGTTAAATACCCTCAGCGTGGCAAAAGTGATTGACGGTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAAc                                                                                                                                                                                                                                 <  1:759073/250‑1 (MQ=255)
     cgccgcCATCAGCGAAACCTCCGTGCAAAATCCACCGGCGTTTGGGCGCGTTCTGTTTATTTTGGTTTTACCGATTTTCCTTATTTTGTTTGATACCGGGTTAAATACCCTCAGCGTGGCAAAAGTGATTGACGGTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCg                                                                                                                                                                                                                           <  1:677977/251‑1 (MQ=255)
                   aaaCCTCCGTGCAAAATCCACCGGCGTTTGGGCGCGTTCTGTTTATTTTGGTTTTACCGATTTTCCTTATTTTGTTTGATGCCGGGTTAAATACCCTCAGCGTGGCAAAAGTGATTGACGGTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACAGTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAAc                                                                                                                                                                                                             <  1:406053/251‑1 (MQ=255)
                   aaaCCTCCGTGCAAAATCCACCGGCGTTTGGGCGCGTTCTGTTTATTTTGGTTTTACCGATTTTCCTTATTTTGTTTGATACCGGGTTAAATACCCTCAGCGTGGCAAAAGTGATTGACGGTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAAc                                                                                                                                                                                                             <  1:612683/251‑1 (MQ=255)
                   aaaCCTCCGTGCAAAATCCACCGGCGTTTGGGCGCGTTCTGTTTATTTTGGTTTTACCGATTTTCCTTATTTTGTTTGATACCGGGTTAAATACCCTCAGCGTGGCAAAAGTGATTGACGGTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAAc                                                                                                                                                                                                             <  1:16871/251‑1 (MQ=255)
                   aaaCCTCCGTGCAAAATCCACCGGCGTTTGGGCGCGTTCTGTTTATTTTGGTTTTACCGATTTTCCTTATTTTGTTTGATACCGGGTTAAATACCCTCAGCGTGGCAAAAGTGATTGACGGTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAAc                                                                                                                                                                                                             <  1:276324/251‑1 (MQ=255)
                                               tGGGCGCGTTCTGTTTATTTTGGTTTTACCGATTTTCCTTATTTTGTTTGATACCGGGTTAAATACCCTCAGCGTGGCAAAAGTGATTGACGGTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCatta                                                                                                                                                                                 <  1:101670/251‑1 (MQ=255)
                                                     cgTTCTGTTTATTTTGGTTTTACCGATTTTCCTTATTTTGTTTGATACCGGGTTAAATACCCTCAGCGTGGCAAAAGTGATTGACGGTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAg                                                                                                                                                                           <  1:241978/251‑1 (MQ=255)
                                                         cTGTTTATTTTGGTTTTACCGATTTTCCTTATTTTGTTTGATACCGGGTTAAATACCCTCAGCGTGGCAAAAGTGATTGACGGTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGa                                                                                                                                                                        >  1:613492/1‑250 (MQ=255)
                                                           gTTTATTTTGGTTTTACCGATTTTCCTTATTTTGTTTGATACCGGGTTAAATGCCCTCAGCGTGGCAAAAGTGATTGGCGGTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCattatt                                                                                                                                                                               <  1:529818/241‑1 (MQ=255)
                                                           gTTTATTTTGGTTTTACCGATTTTCCTTATTTTGTTTGATACCGGGTTAAATACCCTCAGCGTGGCAAAAGTGATTGACGGTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGAc                                                                                                                                                                                                >  1:550024/1‑224 (MQ=255)
                                                           gTTTATTTTGGTTTTACCGATTTTCCTTATTTTGTTTGATACCGGGTTAAATACCCTCAGCGTGGCAAAAGTGATTGACGGTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCg                                                                                                                                                                     >  1:437662/1‑251 (MQ=255)
                                                                                       aTTTTGTTTGATACCGGGTTAAATACCCTCAGCGTGGCAAAAGTGATTGACGGTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAAc                                                                                                                                                                                                             >  1:803093/1‑183 (MQ=255)
                                                                                                 aTACCGGGTTAAATACCCTCAGCGTGGCAAAAGTGATTGACGGTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAg                                                                                                                                <  1:192048/250‑1 (MQ=255)
                                                                                                       ggTTAAATACCCTCAGCGTGGCAAAAGTGATTGACGGTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTgg                                                                                                                                                                                                   <  1:866807/177‑1 (MQ=255)
                                                                                                        gTTAAATACCCTCAGCGTGGCAAAAGTGATTGACGGTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCg                                                                                                                        >  1:86899/1‑251 (MQ=255)
                                                                                                               aCCCTCAGCGTGGCAAAAGTGATTGACGGTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGa                                                                                                                 >  1:730994/1‑251 (MQ=255)
                                                                                                                cccTCAGCGTGGCAAAAGTGATTGACGGTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAg                                                                                                                >  1:305097/1‑251 (MQ=255)
                                                                                                                 ccTCAGCGTGGCAAAAGTGATTGACGGTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGt                                                                                                               <  1:567337/251‑1 (MQ=255)
                                                                                                                         tGGCAAAAGTGATTGACGGTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGc                                                                                                        >  1:605796/1‑250 (MQ=255)
                                                                                                                          ggCAAAAGTGATTGACGGTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCa                                                                                                      >  1:176464/1‑251 (MQ=255)
                                                                                                                            cAAAAGTGATTGACGGTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCAtg                                                                                                    <  1:708898/251‑1 (MQ=255)
                                                                                                                                 gTGATTGACGGTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCAtgttgt                                                                                                >  1:14592/1‑250 (MQ=255)
                                                                                                                                   gATTGACGGTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTgcgggcg                                                                                                                                                            >  1:239940/1‑188 (MQ=255)
                                                                                                                                         cGGTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATAc                                                                                         >  1:72457/1‑249 (MQ=255)
                                                                                                                                           gTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCgg                                                                                      <  1:687545/250‑1 (MQ=255)
                                                                                                                                           gTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGt                                                                                     >  1:763738/1‑251 (MQ=255)
                                                                                                                                                  cTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGc                                                                                                        <  1:817060/225‑1 (MQ=255)
                                                                                                                                                  cTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGTATGc                                                                                 >  1:356696/1‑248 (MQ=255)
                                                                                                                                                  cTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTAATAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTGGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGTATGCCGa                                                                              <  1:95358/251‑1 (MQ=255)
                                                                                                                                                      tgGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGTATGCCGatcat                                                                          <  1:557953/251‑1 (MQ=255)
                                                                                                                                                               aGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCAtgttg                                                                                                 >  1:577074/1‑219 (MQ=255)
                                                                                                                                                                gCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGa                                                                                                                                                                                                 >  1:845415/1‑122 (MQ=255)
                                                                                                                                                                       ccTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGTATGCCGATCATCATCGCCGCCTttatta                                                         <  1:363312/251‑1 (MQ=255)
                                                                                                                                                                       ccTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGTATGCCGATCATCATCGCCGCCTttatta                                                         <  1:41383/251‑1 (MQ=255)
                                                                                                                                                                       ccTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGTATGCCGATCATCATCGCCGCCTttatta                                                         <  1:539705/251‑1 (MQ=255)
                                                                                                                                                                               gCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAg                                                                                                                                                                                                           <  1:534802/97‑1 (MQ=255)
                                                                                                                                                                                 aaaaCCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGTATGCCGATCATCATCGCCGCCTTTATTATTTCTATGGc                                               <  1:394982/251‑1 (MQ=255)
                                                                                                                                                                                           tCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTg                                                                                                                                        >  1:620007/1‑152 (MQ=255)
                                                                                                                                                                                              cgTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGTATGCCGATCATCATCGCCGCCTttatta                                                         <  1:174603/228‑1 (MQ=255)
                                                                                                                                                                                              cgTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGTATGCCGATCATCATCGCCGCCTTTATTATTTCTATGGCATTGCGTGTGGca                                  >  1:297730/1‑251 (MQ=255)
                                                                                                                                                                                                ttattaATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGTATGCCGATCATCATCGCCGCCTTTATTATTTCTATGGCATTGCGTGTGGc                                   >  1:762866/1‑248 (MQ=255)
                                                                                                                                                                                                  attaATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAAc                                                                                                                                                                                                                                 >  1:56620/1‑56 (MQ=255)
                                                                                                                                                                                                  attaATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGc                                                                                                                                                                                                                      <  1:318383/67‑1 (MQ=255)
                                                                                                                                                                                                  attaATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGTATGCCGATCATCATCGCCGCCTTTATTATTTCTATGGCATTGCGTGTGGCACAAg                              >  1:680437/1‑251 (MQ=255)
                                                                                                                                                                                                  attaATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGTATGCCGATCATCATCGCCGCCTTTATTATTTCTATGGCATTGCGTGGGGCACAAg                              >  1:814531/1‑251 (MQ=255)
                                                                                                                                                                                                   ttaATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGTATGCCGATCATCATCGCCGCCTTTATTATTTCTATGGCATTGCGTGTGGCACAAgg                             <  1:531054/251‑1 (MQ=255)
                                                                                                                                                                                                    taATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTAt                                                                                                                                          >  1:702352/1‑141 (MQ=255)
                                                                                                                                                                                                          caTTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGTATGc                                                                                 >  1:519978/1‑192 (MQ=255)
                                                                                                                                                                                                          caTTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGTATGCCGATCATCATCGCCGCCTTTATTATTTCTATGGCATTGCGTGTGGCACAAGGTTCGGCa                      >  1:643324/1‑251 (MQ=255)
                                                                                                                                                                                                              attaATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGTATGCCGATCATCATCGCCGCCTTTATTATTTCTATGGCATTGCGTGTGGCACAAGGTTCGGCaa                     <  1:186713/248‑1 (MQ=255)
                                                                                                                                                                                                              attaATTGCCATTGCCTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGTATGCCGATCATCATCGCCGCCTTTATTATTTCTATGGCATTGCGTGTGGCACAAGGTTCGGCAACgg                  >  1:292717/1‑251 (MQ=255)
                                                                                                                                                                                                                   ttgccattgccTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGTATGCCGATCATCATCGCCGCCTTTATTATTTCTATGGCATTGCGTGTGGCACAAGGTTCGGCAACGGTAGc              >  1:316394/1‑250 (MQ=255)
                                                                                                                                                                                                                    tgccattgccTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGTATGCCGATCATCATCGCCGCCTTTATTATTTCTATGGCATTGCGTGTGGCACAAGGTTCGGCAACGGTAGccc            <  1:774600/251‑1 (MQ=255)
                                                                                                                                                                                                                    tgccattgccTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGTATGCCGATCATCATCGCCGCCTTTATTATTTCTATGGCATTGCGTGTGGCACAAGGTTCGGCAACGGTAGccc            <  1:724236/251‑1 (MQ=255)
                                                                                                                                                                                                                     gccattgccTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAg                                                                                                                                                                                                           >  1:265900/1‑59 (MQ=255)
                                                                                                                                                                                                                         ttgccTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGc                                                                                                        >  1:84871/1‑154 (MQ=255)
                                                                                                                                                                                                                           gccTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTa                                                                                                                                                                                                               >  1:551414/1‑49 (MQ=255)
                                                                                                                                                                                                                           gccTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGTATGCCGATCATCATCGCCGCCTTTATTATTTCTATGGCAt                                             <  1:190113/211‑1 (MQ=255)
                                                                                                                                                                                                                           gccTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGTATGCCGATCATCATCGCCGCCTTTATTATTTCTATGGCATTGCGTGTGGCACAAGGTTCGGCAACGGTAGCCCTGacta      >  1:728649/1‑250 (MQ=255)
                                                                                                                                                                                                                           gccTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGAATGCCGATCATCATCGCCGCCTTTATTATTTCTATGGCATTGCGTGTGGCACAAGGTTCGGCAACGGTAGCCCTGacta      >  1:653001/1‑250 (MQ=255)
                                                                                                                                                                                                                            ccTTATTGCGCGGAGAACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGTATGCCGATCATCATCGCCGCCTTTATTATTTCTATGGCATTGCGTGTGGCACAAGGTTCGGCAACGGTAGCCCTGacta      >  1:852040/1‑249 (MQ=255)
                                                                                                                                                                                                                                        gagaACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGTATGCCGATCATCATCGCCGCCTTTATTATTTCTATGGCATTGCGTGTGGCACAAGGTTCGGCAACGGTAGCCCTGACTACTGc  >  1:790307/1‑241 (MQ=255)
                                                                                                                                                                                                                                          gaACGCGGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTg                                                                                                                                        <  1:446264/105‑1 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                        
AACACCGCCGCCATCAGCGAAACCTCCGTGCAAAATCCACCGGCGTTTGGGCGCGTTCTGTTTATTTTGGTTTTACCGATTTTCCTTATTTTGTTTGATACCGGGTTAAATACCCTCAGCGTGGCAAAAGTGATTGACGGTTCTTCCTTGTGGGTCGAAAGCCTGCGCCTCATCGGCAAAACCCCGATCGCGTTATTAATCACATTATTAATTGCCATTGCCTTATTGCGCGGAGAACGCAGTTATGAACAAATCGAAAGCCTTTGTAACAGTGCGCTGGGACCGATTTGCTCCATTATTTTAGTGACCGGTGCGGGCGGTATGTTCGGTGGCGTATTGCGCGCCAGCGGCATCGGCGATGAGTTATCCGCCATGTTGTCAAATACCGGTATGCCGATCATCATCACCGCCTTTATTATTTCTATGGCATTGCGTGTGGCACAAGGTTCGGCAACGGTAGCCCTGACTACTGC  >  1/482412‑482884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑