Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	482,207	A→G	P142P (CCA→CCG)	unknown →	Low‑affinity gluconate/H+ symporter GntU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	482,207	0	A	G	100.0%	190.3 / NA	72	P142P (CCA→CCG)	unknown	Low‑affinity gluconate/H+ symporter GntU
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/34);  total (38/34)

GGCGTTATTAGTCGGTTTGGGTACCATGATCGGGCGTTTATTGGAAGTCACCGGCGGGGCAAAAGTGCTTGCGGATACGTTGATTAATAAATTCGGCGAAAAGAAAGCGCCTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCAGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTACTTATTTGTTCAGTATGTACATCAGTAAAAAATTTTTTGTGGAACTGCCGAAAACCTTTTTGAACACCGCCGCCATCAGCGAAACCTCCGTGCAAAAT  >  1/481964‑482447                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 ggCGTTATTAGTCGGTTTGGGTACCATGATCGGGCGTTTATTGGAAGTCACCGGCGGGGCAAAAGTGCTTGCGGATACGTTGATTAATAAATTCGGCGAAAAGAAAGCGCCTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGGGGGTCCGTCTTGCGTTATGCCTTCCCGGTTGCCg                                                                                                                                                                                                                                           >  1:545012/1‑251 (MQ=255)    gTTATTAGTCGGTTTGGGTACCATGATCGGGCGTTTATTGGAAGTCACCGGCGGGGCAAAAGTGCTTGCGGATACGTTGATTAATAAATTCGGCGAAAAGAAAGCGCCTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGGTGCCGGTg                                                                                                                                                                                                                                        >  1:156087/1‑251 (MQ=255)       attaGTCGGTTTGGGTACCATGATCGGGCGTTTATTGGAAGTCACCGGCGGGGCAAAAGTGCTTGCGGATACGTTGATTAATAAATTCGGCGAAAAGAAAGCGCCTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGGGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCtt                                                                                                                                                                                                                                     >  1:698103/1‑251 (MQ=255)            tCGGTTTGGGTACCATGATCGGGCGTTTATTGGAAGTCACCGGCGGGGCAAAAGTGCTTGCGGATACGTTGATTAATAAATTCGGCGAAAAGAAAGCGCCTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTg                                                                                                                                                                                                                                  <  1:11219/249‑1 (MQ=255)            tCGGTTTGGGTACCATGATCGGGCGTTTATTGGAAGTCACCGGCGGGGCAAAAGTGCTTGCGGATACGTTGATTAATAAATTCGGCGAAAAGAAAGCGCCTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTg                                                                                                                                                                                                                                  <  1:823170/249‑1 (MQ=255)               gTTTGGGTACCATGATCGGGCGTTTATTGGAAGTCACCGGCGGGGCAAAAGTGCTTGCGGATACGTTGATTAATAAATTCGGCGAAAAGAAAGCGCCTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGGCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGGTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCCTTTGCGGt                                                                                                                                                                                                                              >  1:826992/1‑250 (MQ=255)                   gggTACCATGATCGGGCGTTTATTGGAAGTCACCGGCGGGGCAAAAGTGCTTGCGGATACGTTGATTAATAAATTCGGCGAAAAGAAAGCGCCTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATg                                                                                                                                                                                                                          >  1:476762/1‑250 (MQ=255)                   gggTACCATGATCGGGCGTTTATTGGAAGTCACCGGCGGGGCAAAAGTGCTTGCGGATACGTTGATTAAT‑AATTCGGCGAAAAGAAAGCGCCTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGGTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCa                                                                                                                                                                                                                        >  1:501429/1‑251 (MQ=255)                          aTGATCGGGCGTTTATTGGAAGTCACCGGCGGGGCAAAAGTGCTTGCGGATACGTTGATTTATAAATTCGGCGAAAAGAAAGCGCCTTTTGCGCTGGGGGTTGCCGCGTTGCTGTTCGGCTTCCCG‑GTTTTCTTTTATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCCTTTGCGGTCATGCACGCGtt                                                                                                                                                                                                                  >  1:366325/1‑251 (MQ=255)                             aTCGGGCGTTTATTGGAAGTCACCGGCGGGGCAAAAGAGCTTGCGGGTACGTTGATTTATAAATTCGGCGAAAAGAAAGCGACTTTTGCGCTGGGGCTTGGCGCGTTGATGTTTTGCTTCCCG‑ATTTTTTTTTATGCCGGGCTGGTGGGCATGTTTCCCTTTGTTCTTAGTCTCGGAAAACGTTTTGGTGGGTTCGTCTTGGGTTATGCCTTTCCGGTTGTCGGGGCTTTTGGGGTCATGCACGCGgtttt                                                                                                                                                                                                               >  1:580417/1‑251 (MQ=255)                                     tttATTGGAAGTCACCGGCGGGGCAAAAGTGCTTGCGGATACGTTGATTAATAAATTCGGCGAAAAGAAAGCGCCTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCACAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCAc                                                                                                                                                                                                                       >  1:443288/1‑235 (MQ=255)                                       tATTGGAAGTCACCGGCGGGGCAAAAGTGCTTGCGGATACGTTGATTAATAAATTCGGCGAAAAGAAAGCGCCTTTTGCGCTGGGGGTTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTTGGTGGTTCCGTCTTGCGTTATGCCTTTCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCAt                                                                                                                                                                                                     >  1:712394/1‑251 (MQ=255)                                                tCACCGGCGGGGCAAAAGTGCTTGCGGATACGTTGATTAATAAATTCGGCGAAAAGAAAGCGCCTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTc                                                                                                                                                                                              <  1:262035/249‑1 (MQ=255)                                                           gCAAAAGTGCTTGCGGATACGTTGATTAATAAATTCGGCGAAAAGAAAGCGCCTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTc                                                                                                                                                                                              <  1:396707/238‑1 (MQ=255)                                                                 gTGCTTGCGGATACGTTGATTAATAAATTCGGCGAAAAGAAAGCGCCTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGAttt                                                                                                                                                                           >  1:724483/1‑251 (MQ=255)                                                                     ttGCGGATACGTTGATTAATAAATTCGGCGAAAAGAAAGCGCCTTTTGCGTTGGGGATTGCCGCGTTGCTGTTCGGCTTCCTG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTGGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTAACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTc                                                                                                                                                                       <  1:429149/251‑1 (MQ=255)                                                                      tGCGGATACGTTGATTAATAAATTCGGCGAAAAGAAAGCGCCTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTg                                                                                                                                                                                                                                  <  1:173204/191‑1 (MQ=255)                                                                        cGGATACGTTGATTAATAAATTCGGCGAAAAGAAAGCGCCTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCgg                                                                                                                                                                     >  1:99264/1‑250 (MQ=255)                                                                          gATACGTTGATTAATAAATTCGGCGAAAAGAAAGCGCCTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCgg                                                                                                                                                                  >  1:565643/1‑250 (MQ=255)                                                                             aCGTTGATTAATAAATTCGGCGAAAAGAAAGCGCCTTTTGCGTTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGaa                                                                                                                                                               <  1:57966/251‑1 (MQ=255)                                                                             aCGTTGATTAATAAATTCGGCGAAAAGAAAGCGCCTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTAc                                                                                                                                                                                                             >  1:421972/1‑205 (MQ=255)                                                                             aCGTTGATTAATAAATTCGGCGAAAAGAAAGCGCCTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGaa                                                                                                                                                               >  1:315498/1‑251 (MQ=255)                                                                                ttGATTAATAAATTCGGCGAAAAGAAAGCGCCTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAata                                                                                                                                                             <  1:76148/250‑1 (MQ=255)                                                                                ttGATTAATAAATTCGGCGAAAAGAAAGCGCCTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAata                                                                                                                                                             <  1:745200/250‑1 (MQ=255)                                                                                   aTTAATAAATTCGGCGAAAAGAAAGCGCCTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATggg                                                                                                                                                         <  1:755398/251‑1 (MQ=255)                                                                                    ttAATAAATTCGGCGAAAAGAAAGCGCCTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATggg                                                                                                                                                         >  1:251736/1‑250 (MQ=255)                                                                                    ttAATAAATTCGGCGAAAAGAAAGCGCCTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATggg                                                                                                                                                         >  1:739098/1‑250 (MQ=255)                                                                                                gCGAAAAGAAGGCGTCTTTTGAGATGCGGTTTACCGTGTTGTTGTTC‑GTATCCCGCATTTTCTTTGCTGCCGGTCTGGTGGCCGTGTTGCCTTTCGTCTTTATTGCCGCAAAACCTTGCGGTGGTACTGCCTTGCGTTATGCCTCCCCGGTTGAGGGTGGTTTTGCGGTCATGCACGTGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTAATCGGCGTGAATATGCGCTTGATGGTGAt                                                                                                                                             <  1:550626/250‑1 (MQ=255)                                                                                                gCGAAAAGAAAGCGCCTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTATTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGAt                                                                                                                                             <  1:57821/250‑1 (MQ=255)                                                                                                       gaaagCGCCTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTg                                                                                                                                                                                         <  1:495380/199‑1 (MQ=255)                                                                                                            cgcCTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAAtt                                                                                                                                <  1:8776/251‑1 (MQ=255)                                                                                                              ccTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTg                                                                                                                              >  1:885050/1‑251 (MQ=255)                                                                                                               cTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAatat                                                                                                                                                            <  1:620120/220‑1 (MQ=255)                                                                                                                    gcgcTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGAtt                                                                                                                                            >  1:17104/1‑231 (MQ=255)                                                                                                                    gcgcTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGAt                                                                                                                        >  1:900411/1‑251 (MQ=255)                                                                                                                         ggggATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTAc                                                                                                                                                                                                             >  1:165455/1‑161 (MQ=255)                                                                                                                         ggggATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAata                                                                                                                                                             >  1:644884/1‑209 (MQ=255)                                                                                                                         ggggATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAAtt                                                                                                                                >  1:174725/1‑238 (MQ=255)                                                                                                                            gATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGAcc                                                                                                                 <  1:482145/250‑1 (MQ=255)                                                                                                                            gATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGAcc                                                                                                                 <  1:758541/250‑1 (MQ=255)                                                                                                                                      ttGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCgg                                                                                                      >  1:553432/1‑251 (MQ=255)                                                                                                                                         cTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTAc                                                                                                   <  1:544247/251‑1 (MQ=255)                                                                                                                                              cGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTACTTAtt                                                                                              <  1:627809/251‑1 (MQ=255)                                                                                                                                                 cTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTACTTATTTGt                                                                                           <  1:522759/251‑1 (MQ=255)                                                                                                                                                         tttttCTTTGATGCCGGTCTGGTGGTCATGTTGCCTTTCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTACTTATTTGTTCAGTAt                                                                                    <  1:348137/250‑1 (MQ=255)                                                                                                                                                         aTTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCt                                                                                                                                                       >  1:695467/1‑184 (MQ=255)                                                                                                                                                              cTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCTAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGATTATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTACTTATTTGTTCAGTATGTACa                                                                               >  1:513180/1‑251 (MQ=255)                                                                                                                                                              cTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAACCGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGTTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTACTTATTTGTTCAGTATGTACa                                                                               <  1:88883/251‑1 (MQ=255)                                                                                                                                                              cTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTACTTATTTGTTCAGTATGTACa                                                                               <  1:106516/251‑1 (MQ=255)                                                                                                                                                              cTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTACTTATTTGTTCAGTATGTACa                                                                               <  1:309462/251‑1 (MQ=255)                                                                                                                                                                ttGATGCCGTTGTGGGGGTCAGTTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTGGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTGCCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTACTTATTTGTTCAGTATGTACATc                                                                             <  1:195441/251‑1 (MQ=255)                                                                                                                                                                ttGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTACTTATTTGTTCAGTATGTACATc                                                                             <  1:463352/251‑1 (MQ=255)                                                                                                                                                                   aTGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTACTTAtt                                                                                              <  1:699523/231‑1 (MQ=255)                                                                                                                                                                   aTGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTACTTATTTGTTCAGTATGTACATCAg                                                                           <  1:846813/250‑1 (MQ=255)                                                                                                                                                                             ggtggtCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTACTTATTTGTTCAGTATGTACATc                                                                             >  1:107689/1‑238 (MQ=255)                                                                                                                                                                             ggtggtCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCCTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTACTTATTTGTTCAGTATGTACATc                                                                             >  1:884244/1‑238 (MQ=255)                                                                                                                                                                                 gtCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTACTTATTTGTTCAGTATGTACATCAGTAAAAAATTTTTtgt                                                            >  1:661103/1‑251 (MQ=255)                                                                                                                                                                                  tCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTc                                                                                                                                                                       >  1:24185/1‑143 (MQ=255)                                                                                                                                                                                        tGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTACTTATTTGTTCAGTATGTACATCAGTAAAAAATTTTTTGTGGaa                                                        >  1:836550/1‑248 (MQ=255)                                                                                                                                                                                         gCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGAcc                                                                                                                 <  1:6635/190‑1 (MQ=255)                                                                                                                                                                                             aTCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGc                                                                                                                                                        <  1:177962/147‑1 (MQ=255)                                                                                                                                                                                                gTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTACTTATTTGTTCAGTATGTACa                                                                               <  1:592357/217‑1 (MQ=255)                                                                                                                                                                                                           cGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTACTTATTTGTTCAGTATGTACATCAGTAAAAAATTTTTTGTGGAACTGCCGAAAACCTTTTTGAaca                                  >  1:833792/1‑251 (MQ=255)                                                                                                                                                                                                            gCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTACTTATTTGTTCAGTATGTACATCAGTAAAAAATTTTTTGTGGAACTGCCGAAAACCTTTTTGAaca                                  >  1:371374/1‑250 (MQ=255)                                                                                                                                                                                                                    tttCGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACGTCGGTAc                                                                                                   >  1:446388/1‑177 (MQ=255)                                                                                                                                                                                                                       cGGTGGTTCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTACTTATTTGTTCAGTATGTACa                                                                               >  1:794097/1‑194 (MQ=255)                                                                                                                                                                                                                              tCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTAc                                                                                                   >  1:847261/1‑167 (MQ=255)                                                                                                                                                                                                                              tCCGTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTACTTATTTGTTCAGTATGTACATCAGTAAAAAATTTTTTGTGGAACTGCCGAAAACCTTTTTGAACACCGCCGCCATCAGCGAAAc               <  1:387776/251‑1 (MQ=255)                                                                                                                                                                                                                                 gTCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTACTTATTTGTTCAGTATGTACATCAGTAAAAAATTTTTTGTGGAACTGCCGAAAACCTTTTTGAACACCGCCGCCATCAGCGAAACCt             >  1:612541/1‑250 (MQ=255)                                                                                                                                                                                                                                  tCTTGCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTACTTATTTGTTCAGTATGTACATCAGTAAAAAATTTTTTGTGGAACTGCCGAAAACCTTTTTGAACACCGCCGCCATCAGCGAAACCTc            <  1:688252/250‑1 (MQ=255)                                                                                                                                                                                                                                      gCGTTATGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTACTTATTTGTTCAGTATGTACATCAGTAAAAAATTTTTTGTGGAACTGCCGAAAACCTTTTTGAACACCGCCGCCATCAGCGAAACCTCCGTg        >  1:364201/1‑250 (MQ=255)                                                                                                                                                                                                                                           aTGCCTTCCCGGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTACTTATTTGTTCAGTATGTACATCAGTAAAAAATTTTTTGTGGAACTGCCGAAAACCTTTTTGAACACCGCCGCCATCAGCGAAACCTCCGTGCAAAAt  <  1:497710/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 GGCGTTATTAGTCGGTTTGGGTACCATGATCGGGCGTTTATTGGAAGTCACCGGCGGGGCAAAAGTGCTTGCGGATACGTTGATTAATAAATTCGGCGAAAAGAAAGCGCCTTTTGCGCTGGGGATTGCCGCGTTGCTGTTCGGCTTCCCG‑ATTTTCTTTGATGCCGGTCTGGTGGTCATGTTGCCTATCGTCTTTAGTGTCGCAAAACGTTTCGGTGGTTCCGTCTTGCGTTATGCCTTCCCAGTTGCCGGTGCTTTTGCGGTCATGCACGCGTTTTTACCGCCGCATCCGGGTCCGGTGGCTTCCGGTGATTTACTCGGCGTGAATATGGGCTTGATGGTGATTGTTGGCTTAATTTGCGCGATCCCGACCTGGTACATCGGTACTTATTTGTTCAGTATGTACATCAGTAAAAAATTTTTTGTGGAACTGCCGAAAACCTTTTTGAACACCGCCGCCATCAGCGAAACCTCCGTGCAAAAT  >  1/481964‑482447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑