Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	449,544	A→C	*78S (TAA→TCA)	unknown →	Hypothetical metal‑binding enzyme, YcbL homolog

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	449,544	0	A	C	100.0%	213.6 / NA	75	*78S (TAA→TCA)	unknown	Hypothetical metal‑binding enzyme, YcbL homolog
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (37/38);  total (37/38)

GGTCATTATGAATATTGAAATTATTCCCGTCACCGCATTTCAACAAAATTGTTCGTTAATTTGGGATGATAAGAAAAATGCGGCAATTATTGATCCCGGCGGCGAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTAAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGACACACGCCGGGGCATATCGGTTTTATCGAACATCAAAAACGTGTGGCGTTTACCGGTGATGTGTTATTTAAAAG  >  1/449305‑449781                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                              ggTCATTATGAATATTGAAATTATTCCCGTCACCGCATTTCAACAAAATTGTTCGTTAATTTGGGATGATAAGAAAAATGCGGCAATTATTGATCCCGGCGGCGAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAg                                                                                                                                                                                                                                    >  1:402421/1‑251 (MQ=255)  gTCATTATGAATATTGAAATTATTCCCGTCACCGCATTTCAACAAAATTGTTCGTTAATTTGGGATGATAAGAAAAATGCGGCAATTATTGATCCCGGCGGCGAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAga                                                                                                                                                                                                                                   >  1:858599/1‑251 (MQ=255)          gAATATTGAAATTATTCCCGTCACCGCATTTCAACAAAATTGTTCGTTAATTTGGGATGATAAGAAAAATGCGGCAATTATTGATCCCGGCGGCGAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGattatt                                                                                                                                                                                                                           >  1:691299/1‑251 (MQ=255)            atatTGAAATTATTCCCGTCACCGCATTTCAACAAAATTGTTCGTTAATTTGGGATGATAAGAAAAATGCGGCAATTATTGATCCCGGCGGCGAAGCGGATAAATTACTCAAACGAATTGAAGAATTAGGCTTGAATCTACAGGCGATTTTACTCACTCATGGGTATTTGGATCATGCGGGCGCGGCGGAAAAAGTCAAACAACAGTTTCATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTgg                                                                                                                                                                                                                         >  1:577626/1‑251 (MQ=255)            atatTGAAATTATTCCCGTCACCGCATTTCAACAAAATTGTTCGTTAATTTGGGATGATAAGAAAAATGCGGCAATTATTGATCCCGGCGGCGAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTgg                                                                                                                                                                                                                         <  1:640129/251‑1 (MQ=255)                 gAAATTATTCCCGTCACCGCATTTCAACAAAATTGTTCGTTAATTTGGGATGATAAGAAAAATGCGGCAATTATTGATCCCGGCGGCGAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTaa                                                                                                                                                                                                                    >  1:374271/1‑251 (MQ=255)                   aaTTATTCCCGTCACCGCATTTCAACAAAATTGTTCGTTAATTTGGGATGATAAGAAAAATGCGGCAATTATTGATCCCGGCGGCGAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAgg                                                                                                                                                                                                                  >  1:72638/1‑251 (MQ=255)                     ttattCCCGTCACCGCATTTCAACAAAATTGTTCGTTAATTTGGGATGATAAGAAAAATGCGGCAATTATTGATCCCGGCGGCGAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAggg                                                                                                                                                                                                                 >  1:81965/1‑250 (MQ=255)                            cGTCACCGCATTTCAACAAAATTGTTCGTTAATTTGGGATGATAAGAAAAATGCGGCAATTATTGATCCCGGCGGCGAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCg                                                                                                                                                                                                          >  1:676421/1‑250 (MQ=255)                                        tCAACAAAATTGTTCGTTAATTTGGGATGATAAGAAAAATGCGGCAATTATTGATCCCGGCGGCGAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGaaa                                                                                                                                                                                             <  1:267528/251‑1 (MQ=255)                                           acaaAATTGTTCGTTAATTTGGGATGATAAGAAAAATGCGGCAATTATTGATCCCGGCGGCGAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAAt                                                                                                                                                                                          >  1:823466/1‑251 (MQ=255)                                                      cGTTAATTTGGGATGATAAGAAAAATGCGGCAATTATTGATCCCGGCGGCGAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGAtgttg                                                                                                                                                                               >  1:118445/1‑251 (MQ=255)                                                          aaTTTGGGATGATAAGAAAAATGCGGCAATTATTGATCCCGGCGGCGAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTg                                                                                                                                                                           >  1:898545/1‑251 (MQ=255)                                                          aaTTTGGGATGATAAGAAAAATGCGGCAATTATTGATCCCGGCGGCGAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTg                                                                                                                                                                           >  1:411807/1‑251 (MQ=255)                                                          aaTTTGGGATGATAAGAAAAATGCGGCAATTATTGATCCCGGCGGCGAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTg                                                                                                                                                                           >  1:275298/1‑251 (MQ=255)                                                          aaTTTGGGATGATAAGAAAAATGCGGCAATTATTGATCCCGGCGGCGAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCACTTGGTTCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTg                                                                                                                                                                           >  1:174419/1‑251 (MQ=255)                                                              tGGGATGATAAGAAAAATGCGGCAATTATTGATCCCGGCGGCGAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCCTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGt                                                                                                                                                                       <  1:441921/251‑1 (MQ=255)                                                                ggATGATAAGAAAAATGCGGCAATTATTGATCCCGGCGGCGAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAgg                                                                                                                                                                                                                  <  1:189587/206‑1 (MQ=255)                                                                    gatAAGAAAAATGCGGCAATTATTGATCCCGGCGGCGAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTcgccg                                                                                                                                                                  >  1:238654/1‑250 (MQ=255)                                                                            aaaTGCGGCAATTATTGATCCCGGCGGCGAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAAc                                                                                                                                                         <  1:312175/251‑1 (MQ=255)                                                                                   gCAATTATTGATCCCGGCGGCGAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCg                                                                                                                                                  >  1:700177/1‑251 (MQ=255)                                                                                       ttattGATCCCGGCGGCGAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGcc                                                                                                                                                                                                           <  1:36022/190‑1 (MQ=255)                                                                                       ttattGATCCCGGCGGCGAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCgttgg                                                                                                                                              >  1:249601/1‑251 (MQ=255)                                                                                        tattGATCCCGGCGGCGAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCgttggt                                                                                                                                             <  1:162828/251‑1 (MQ=255)                                                                                          ttGATCCCGGCGGCGAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCgttggttg                                                                                                                                           <  1:127882/251‑1 (MQ=255)                                                                                                 cggcggCGAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATaa                                                                                                                                      >  1:815641/1‑249 (MQ=255)                                                                                                    cggcgAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAaagaa                                                                                                                                  <  1:211433/250‑1 (MQ=255)                                                                                                           gCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAAt                                                                                                                          >  1:558429/1‑251 (MQ=255)                                                                                                             ggATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAggg                                                                                                                                                                                                                 >  1:570158/1‑162 (MQ=255)                                                                                                             ggATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAAttt                                                                                                                        >  1:682013/1‑251 (MQ=255)                                                                                                                 aaaTTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATaa                                                                                                                                      >  1:174670/1‑233 (MQ=255)                                                                                                                 aaaTTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTaa                                                                                                                     >  1:663694/1‑250 (MQ=255)                                                                                                                  aaTTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACt                                                                                                                   <  1:25317/251‑1 (MQ=255)                                                                                                                   aTTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTa                                                                                                                  <  1:641420/251‑1 (MQ=255)                                                                                                                           aaCGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGAt                                                                                                          <  1:471674/251‑1 (MQ=255)                                                                                                                              gAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGAtttt                                                                                                       >  1:559652/1‑251 (MQ=255)                                                                                                                                 ttGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGAACATGTGGGCGCGGCGGAAAAAGACAAACAACAGCTTAATGTGCCCATCTTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTAc                                                                                                     >  1:729786/1‑250 (MQ=255)                                                                                                                                      gaaTTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCgttgg                                                                                                                                              <  1:283344/204‑1 (MQ=255)                                                                                                                                       aaTTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTATTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGaa                                                                                              >  1:435855/1‑251 (MQ=255)                                                                                                                                        aTTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAg                                                                                             <  1:539600/251‑1 (MQ=255)                                                                                                                                                           gCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCggg                                                                             <  1:579415/248‑1 (MQ=255)                                                                                                                                                            cGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGacac                                                                         <  1:26700/251‑1 (MQ=255)                                                                                                                                                              aTTTTACTCACTCATGGGCAGTTGGATCATGTGGGCGCGGCAGAAAAAGTCAAACATCAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTGTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGacacac                                                                       <  1:377427/251‑1 (MQ=255)                                                                                                                                                                      cactcaTGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGACACACGCCGGGGc                                                               <  1:72438/251‑1 (MQ=255)                                                                                                                                                                      cactcaTGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGACACACGCCGGGGc                                                               <  1:642244/251‑1 (MQ=255)                                                                                                                                                                        ctcaTGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGAt                                                                                                                                                    >  1:8361/1‑164 (MQ=255)                                                                                                                                                                          caTGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGACACACg                                                                      >  1:615196/1‑240 (MQ=255)                                                                                                                                                                           aTGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGACACACGCCGGGGCATATc                                                          <  1:214314/251‑1 (MQ=255)                                                                                                                                                                              ggCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGACACACGCCGGGGCATATCgg                                                        <  1:392496/250‑1 (MQ=255)                                                                                                                                                                                cATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTAcc                                                                                <  1:651266/224‑1 (MQ=255)                                                                                                                                                                                     ggATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACt                                                                                                                   <  1:196042/184‑1 (MQ=255)                                                                                                                                                                                     ggATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGACACACGCCGGGGCATATCGGTTTTATCg                                                >  1:571231/1‑251 (MQ=255)                                                                                                                                                                                      gATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGACACACGCCGGGGCATATCgg                                                        <  1:520810/242‑1 (MQ=255)                                                                                                                                                                                      gATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGACACACGCCGGGGCATATCGGTTTTATCGa                                               >  1:13020/1‑251 (MQ=255)                                                                                                                                                                                        tCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGACACACGCCGGGGCATATCGGTTTTATCGAAc                                             <  1:65513/251‑1 (MQ=255)                                                                                                                                                                                                 cgcgGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGACACACGCCGGGGCATATCGGTTTTATCGAACATCaaaaa                                     <  1:126961/250‑1 (MQ=255)                                                                                                                                                                                                              gTCAAACAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGACACACGcc                                                                    >  1:483513/1‑206 (MQ=255)                                                                                                                                                                                                                 aaaCAACAGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGACACACGCCGGGGCATATCGGTTTTATCGAACATCAAAAACGTGTGGCGTTTACCgg                    <  1:281251/251‑1 (MQ=255)                                                                                                                                                                                                                 aaaCAACAGTTTAATGTGCCCAACCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGACACACGCCGGGGCATATCGGTTTTATCGAACATCAAAAACGTGTGGCGTTTACCgg                    <  1:826686/251‑1 (MQ=255)                                                                                                                                                                                                                      acaGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGACACACGCCGGGGCATATCGGTTTTATCGAACATCAAAAACGTGTGGCGTTTACCGGTGAtg               >  1:359917/1‑251 (MQ=255)                                                                                                                                                                                                                       caGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGacacac                                                                       >  1:255952/1‑194 (MQ=255)                                                                                                                                                                                                                        aGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACt                                                                                                                   <  1:515081/149‑1 (MQ=255)                                                                                                                                                                                                                        aGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAGGTTCTGCATTTACCGGGacac                                                                         >  1:430516/1‑191 (MQ=255)                                                                                                                                                                                                                        aGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGACACACGCCGGGGCATATCGGttt                                                     >  1:858164/1‑211 (MQ=255)                                                                                                                                                                                                                        aGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGACACACGCCGGGGCATATCGGTTTTATCGAACATCAAAAACGTGTGGCGTTTAcc                      <  1:337668/242‑1 (MQ=255)                                                                                                                                                                                                                        aGTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGACACACGCCGGGGCATATCGGTTTTATCGAACATCAAAAACGTGTGGCGTTTACCGGTGAtgtg             >  1:267177/1‑251 (MQ=255)                                                                                                                                                                                                                         gTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGACACACGCCGGGGCATATCGGTTTTATCGAACATCAAAAACg                                   <  1:898306/228‑1 (MQ=255)                                                                                                                                                                                                                         gTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGACACACGCCGGGGCATATCGGTTTTATCGAACATCAAAAACGTGTGGCGTTTAcc                      <  1:882100/241‑1 (MQ=255)                                                                                                                                                                                                                         gTTTAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGACACACGCCGGGGCATATCGGTTTTATCGAACATCAAAAACGTGTGGCGTTTACCgg                    <  1:851103/243‑1 (MQ=255)                                                                                                                                                                                                                          tttAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGACACACGCCGGGGCATATCGGTTTTATCGAACATCAAAAACGTGTGGCGTTTACCGGTGAtgtg             <  1:229086/249‑1 (MQ=255)                                                                                                                                                                                                                          tttAATGTGCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGACACACGCCGGGGCATATCGGTTTTATCGAACATCAAAAACGTGTGGCGTTTACCGGTGAtg               <  1:116203/247‑1 (MQ=255)                                                                                                                                                                                                                                  gCCCATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAAtt                                                                                                                         <  1:274528/133‑1 (MQ=255)                                                                                                                                                                                                                                   cccATCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGACACACGCCGGGGCATATCGGTTTTATCGAACATCAAAAACGTGTGGCGTTTACCGGTGATGTGTTATTTAAAAg  <  1:15847/251‑1 (MQ=255)                                                                                                                                                                                                                                       tCCTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGACACACGCCGGGGCATATCGGTTTTATCGAACATCAAAAACg                                   >  1:168178/1‑214 (MQ=255)                                                                                                                                                                                                                                        ccTCGGTTCAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGACACACGCCGGGGCATATCGGTTTTATCGAACATCAAAAACGTGTGGCGTTTACCGGTGAtg               <  1:290673/233‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                              GGTCATTATGAATATTGAAATTATTCCCGTCACCGCATTTCAACAAAATTGTTCGTTAATTTGGGATGATAAGAAAAATGCGGCAATTATTGATCCCGGCGGCGAAGCGGATAAATTAATCAAACGAATTGAAGAATTAGGCTTGAATTTACAGGCGATTTTACTCACTCATGGGCATTTGGATCATGTGGGCGCGGCGGAAAAAGTCAAACAACAGTTTAATGTGCCCATCCTCGGTTAAAATTCCAAAGATGATTATTGGTTTAAGGGCTTGCCGCAACAATCGGAAAAATTCGGGATGTTGTTTGAAGTCGCCGCCTTTGAACCGGATCGTTGGTTGGATAAAGAAGGCGAAATTTTAACTATCGGCGATTTTACCTTTGAAGTTCTGCATTTACCGGGACACACGCCGGGGCATATCGGTTTTATCGAACATCAAAAACGTGTGGCGTTTACCGGTGATGTGTTATTTAAAAG  >  1/449305‑449781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑