Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	443,672	C→T	R132Q (CGG→CAG)	unknown ←	Inner membrane protein YebS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	443,672	0	C	T	100.0%	216.3 / NA	79	R132Q (CGG→CAG)	unknown	Inner membrane protein YebS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/39);  total (40/39)

TGACCGCTTTGCTTTGCGGGTAAAAATCGTTCCACACTTCGTCGGGGTTAATTTTAATAAACAAGAGCGTGGTTAAAAGTGCGGTAAAAATAAAAGCGATTAAGTAAATATTCAGTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCCGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATGCTTAAAAGCGGGTAAGTTAAAGCGAAAGGCATCAGGATTAAAATAGAGATGGCGATAATCGAACAGCGTTTTAAACTCCAGCGG  >  1/443427‑443914                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   tGACCGCTTTGCTTTGCGGGTAAAAATCGTTCCACACTTCGTCGGGGTTAATTTTAATAAACAAGAGCGTGGTTAAAAGTGCGGTAAAAATAAAAGCGATTAAGTAAATATTCAGTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGAc                                                                                                                                                                                                                                               <  1:532199/251‑1 (MQ=255) tGACCGCTTTGCTTTGCGGGTAAAAATCGTTCCACACTTCGTCGGGGTTAATTTTAATAAACAAGAGCGTGGTTAAAAGTGCGGTAAAAATAAAAGCGATTAAGTAAATATTCAGTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGAc                                                                                                                                                                                                                                               <  1:180149/251‑1 (MQ=255) tGACCGCTTTGCTTTGCGGGTAAAAATCGTTCCACACTTCGTCGGGGTTAATTTTAATAAACAAGAGCGTGGTTAAAAGTGCGGTAAAAATAAAAGCGATTAAGTAAATATTCAGTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGAc                                                                                                                                                                                                                                               <  1:736963/251‑1 (MQ=255) tGACCGCTTTGCTTTGCGGGTAAAAATCGTTCCACACTTCGTCGGGGTTAATTTTAATAAACAAGAGCGTGGTTAAAAGTGCGGTAAAAATAAAAGCGATTAAGTAAATATTCAGTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGAc                                                                                                                                                                                                                                               <  1:338160/251‑1 (MQ=255)     cGCTTTGCTTTGCGGGTAAAAATCGTTCCACACTTCGTCGGGGTTAATTTTAATAAACAAGAGCGTGGTTAAAAGTGCGGTAAAAATAAAAGCGATTAAGTAAATATTCAGTCCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGcc                                                                                                                                                                                                                                           <  1:835067/251‑1 (MQ=255)               tgcGGGTAAAAATCGTTCCACACTTCGTCGGGGTTAATTTTAATAAACAAGAGCGTGGTTAAAAGTGCGGTAAAAATAAAAGCGATTAAGTAAATATTCAGTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAAt                                                                                                                                                                                                                                 <  1:875455/251‑1 (MQ=255)                 cGGGTAAAAATCGTTCCACACTTCGTCGGGGTTAATTTTAATAAACAAGAGCGTGGTTAAAAGTGCGGTAAAAATAAAAGCGATTAAGTAAATATTCAGTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATaa                                                                                                                                                                                                                               <  1:193143/251‑1 (MQ=255)                    gTAAAAATCGTTCCACACTTCGTCGGGGTTAATTTTAATAAACAAGAGCGTGGTTAAAAGTGCGGTAAAAATAAAAGCGATTAAGTAAATATTCAGTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAAcc                                                                                                                                                                                                                             <  1:714562/250‑1 (MQ=255)                                   cacTTCGTCGGGGTTAATTTTAATAAACAAGAGCGTGGTTAAAAGTGCGGTAAAAATAAAAGCGATTAAGTAAATATTCAGTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGAc                                                                                                                                                                                                                                               <  1:664034/217‑1 (MQ=255)                                   cacTTCGTCGGGGTTAATTTTAATAAACAAGAGCGTGGTTAAAAGTGCGGTAAAAATAAAAGCGATTAAGTAAATATTCAGTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGAc                                                                                                                                                                                                                                               <  1:799352/217‑1 (MQ=255)                                        cgtcgGGGTTAATTTTAATAAACAAGAGCGTGGTTAAAAGTGCGGTAAAAATAAAAGCGATTAAGTAAATATTCAGTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGc                                                                                                                                                                                                        <  1:656218/251‑1 (MQ=255)                                                       taataaACAAGAGCGTGGTTAAAAGTGCGGTAAAAATAAAAGCGATTAAGTAAATATTCAGTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAac                                                                                                                                                                                                  >  1:105551/1‑242 (MQ=255)                                                        aataaACAAGAGCGTGGTTAAAAGTGCGGTAAAAATAAAAGCGATTAAGTAAATATTCAGTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCaaa                                                                                                                                                                                        >  1:390271/1‑251 (MQ=255)                                                             aCAAGAGCGTGGTTAAAAGTGCGGTAAAAATAAAAGCGATTAAGTAAATATTCAGTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGaaa                                                                                                                                                                                    >  1:255913/1‑250 (MQ=255)                                                              cAAGAGCGTGGTTAAAAGTGCGGTAAAAATAAAAGCGATTAAGTAAATATTCAGTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAaca                                                                                                                                                                                  >  1:892711/1‑251 (MQ=255)                                                                       ggTTAAAAGTGCGGTAAAAATAAAAGCGATTAAGTAAATATTCAGTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAac                                                                                                                                                                                                  <  1:7449/226‑1 (MQ=255)                                                                         ttAAAAGTGCGGTAAAAATAAAAGCGATTAAGTAAATATTCAGTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGc                                                                                                                                                                        <  1:229807/250‑1 (MQ=255)                                                                                        aaaTAAAAGCGATTAAGTAAATATTCAGTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGcc                                                                                                                                                                                                                                           >  1:370782/1‑168 (MQ=255)                                                                                                 cGATTAAGTAAATATTCAGTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGc                                                                                                                                                >  1:729776/1‑250 (MQ=255)                                                                                                        gTAAATATTCAGTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTc                                                                                                                                         >  1:269679/1‑250 (MQ=255)                                                                                                           aaTATTCAGTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATAGAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCcaca                                                                                                                                     <  1:383343/251‑1 (MQ=255)                                                                                                              atTCAGTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAac                                                                                                                                                                                                  <  1:416955/187‑1 (MQ=255)                                                                                                              atTCAGTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACg                                                                                                                                   >  1:607431/1‑250 (MQ=255)                                                                                                                 cAGTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAGATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGaa                                                                                                                                                              >  1:482691/1‑220 (MQ=255)                                                                                                                   gTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGcc                                                                                                                                                                                                                                           >  1:680178/1‑141 (MQ=255)                                                                                                                   gTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCGATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCccag                                                                                                                              >  1:423639/1‑250 (MQ=255)                                                                                                                   gTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCccag                                                                                                                              >  1:775362/1‑250 (MQ=255)                                                                                                                         aTGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAAc                                                                                                                                                          <  1:314105/216‑1 (MQ=255)                                                                                                                             ggCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGa                                                                                                                                                                                      >  1:22278/1‑184 (MQ=255)                                                                                                                              gCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAgg                                                                                                                   <  1:446260/250‑1 (MQ=255)                                                                                                                                   cTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCt                                                                                                                                                        <  1:511008/208‑1 (MQ=255)                                                                                                                                   cTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCccagcc                                                                                                                            <  1:371936/236‑1 (MQ=255)                                                                                                                                   cTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCaa                                                                                                             >  1:691759/1‑251 (MQ=255)                                                                                                                                    tCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAAt                                                                                                            <  1:624206/251‑1 (MQ=255)                                                                                                                                           cTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCg                                                                                                     >  1:479909/1‑251 (MQ=255)                                                                                                                                           cTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCg                                                                                                     >  1:331336/1‑251 (MQ=255)                                                                                                                                             ttGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGc                                                                                                    >  1:650734/1‑250 (MQ=255)                                                                                                                                                      cTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATc                                                                                          <  1:169037/251‑1 (MQ=255)                                                                                                                                                          cGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGaa                                                                                                                                                              >  1:200435/1‑179 (MQ=255)                                                                                                                                                           gCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATg                                                                                      >  1:730308/1‑250 (MQ=255)                                                                                                                                                            cccAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGc                                                                                                                                                                        <  1:185150/167‑1 (MQ=255)                                                                                                                                                                   ccaccaAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATGCTTAAAAg                                                                              <  1:22377/250‑1 (MQ=255)                                                                                                                                                                   ccaccaAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATGCTTAAAAg                                                                              <  1:786338/250‑1 (MQ=255)                                                                                                                                                                     accaAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAac                                                                                                                                                                                                  >  1:695008/1‑132 (MQ=255)                                                                                                                                                                       caAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGaa                                                                                                                                                              <  1:661378/166‑1 (MQ=255)                                                                                                                                                                               cGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGAc                                                                                                                                                                                                                                               >  1:754529/1‑77 (MQ=255)                                                                                                                                                                               cGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGaa                                                                                                                                                              <  1:342046/158‑1 (MQ=255)                                                                                                                                                                                        tCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATGCTTAAAAGCGGGTAAGTTAAAGCGAAAGGc                                                        <  1:616079/251‑1 (MQ=255)                                                                                                                                                                                               gggTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAac                                                                                                                                                                                                  >  1:319517/1‑106 (MQ=255)                                                                                                                                                                                                 gTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAac                                                                                                                                                                                                  >  1:794066/1‑104 (MQ=255)                                                                                                                                                                                                 gTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATc                                                                                          >  1:898924/1‑208 (MQ=255)                                                                                                                                                                                                 gTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATGCt                                                                                    <  1:319256/214‑1 (MQ=255)                                                                                                                                                                                                 gTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATGCTTAAAAGCGGGTAAGTTAAAGCGAAAGGCATCAGGAtt                                               >  1:891009/1‑251 (MQ=255)                                                                                                                                                                                                 gTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATGCTTAAAAGCGGGTAAGTTAAAGCGAAAGGCATCAGGAtt                                               >  1:728455/1‑251 (MQ=255)                                                                                                                                                                                                    tAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAAtt                                                                                                           <  1:841409/188‑1 (MQ=255)                                                                                                                                                                                                    tAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATc                                                                                          <  1:16289/205‑1 (MQ=255)                                                                                                                                                                                                    tAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATGCTTaaa                                                                                >  1:279589/1‑215 (MQ=255)                                                                                                                                                                                                    tAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATGCTTAAAAGCGGGTAAGTTAAAGCGAAAGGCATCAGGATTaaa                                            >  1:356659/1‑251 (MQ=255)                                                                                                                                                                                                    tAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATGCTTAAAAGCGGGTAAGTTAAAGCGAAAGGCATCAGGATTaaa                                            >  1:352388/1‑251 (MQ=255)                                                                                                                                                                                                    tAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATGCTTAAAAGCGGGTAAGTTAAAGCGAAAGGCATCAGGATTaaa                                            >  1:620041/1‑251 (MQ=255)                                                                                                                                                                                                    tAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATGCTTAAAAGCGGGTAAGTTAAAGCGAAAGGCATCAGGATTaaa                                            >  1:788460/1‑251 (MQ=255)                                                                                                                                                                                                     aaTATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCTATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATGCTTAAAAGCGGGTAAGTTAAAGCGAAAGGCATCAGGATTaaaa                                           <  1:50585/251‑1 (MQ=255)                                                                                                                                                                                                      atatAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCTCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATGCTTAAAAGCGGGTAAGTTAAAGCGAAAGGCATCAGGATTAAAAt                                          <  1:121563/251‑1 (MQ=255)                                                                                                                                                                                                      atatAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAAt                                                                                                                                                                          >  1:11472/1‑123 (MQ=255)                                                                                                                                                                                                      atatAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATGCTTAAAAGCGGGTAAGTTAAAGCGAAAGGCATCAGGATTAAAAt                                          >  1:358884/1‑251 (MQ=255)                                                                                                                                                                                                      atatAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCACGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAg                                                                                                                    >  1:260856/1‑177 (MQ=255)                                                                                                                                                                                                         taACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATGCTTAAAAGCGGGTAAGTTAAAg                                                                <  1:364369/226‑1 (MQ=255)                                                                                                                                                                                                          aaCTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATc                                                                                          <  1:494545/199‑1 (MQ=255)                                                                                                                                                                                                          aaCTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATGCTTAAAAGCGGGTAAGTTAAAGCGAAAGGCATCAGGATTAAAATagag                                      <  1:735540/251‑1 (MQ=255)                                                                                                                                                                                                          aaCTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATGCTTAAAAGCGGGTAAGTTAAAGCGAAAGGCATCAGGATTAAAATagag                                      <  1:748320/251‑1 (MQ=255)                                                                                                                                                                                                           aCTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATGCTTAAAAGCGGGTAAGTTAAAGCGAAAGGCATCAGGATTAAAATAgaga                                     <  1:690983/251‑1 (MQ=255)                                                                                                                                                                                                            cTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGaa                                                                                                                                                              >  1:821900/1‑129 (MQ=255)                                                                                                                                                                                                                                    ggTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATGCTTAAAAGCGGGTAAGTTAAAGCGAAAGGCATCAGGATTAAAATAGAGATGGCGATAATCGAACAGCGTTTTaa            >  1:856119/1‑251 (MQ=255)                                                                                                                                                                                                                                     gTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATGCTTAAAAGCGGGTAAGTTAAAGCGAAAGGCATCAGGATTAAAATAgaga                                     >  1:231546/1‑225 (MQ=255)                                                                                                                                                                                                                                     gTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATGCTTAAAAGCGGGTAAGTTAAAGCGAAAGGCATCAGGATTAAAATAGAGATGGCGATAATCGAAc                      <  1:272987/240‑1 (MQ=255)                                                                                                                                                                                                                                     gTTTTATGTGCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATGCTTAAAAGCGGGTAAGTTAAAGCGAAAGGCATCAGGATTAAAATAGAGATGGCGATAATCGAACAGCGTTTTaaa           >  1:773327/1‑251 (MQ=255)                                                                                                                                                                                                                                            gtgCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATGCTTAAAAGCGGGTAAGTTAAAGCGAAAGGCATCAGGATTAAAATAGAGATGGCGATAATCGAACAGCGTTTTAAACTCCAg     >  1:845122/1‑250 (MQ=255)                                                                                                                                                                                                                                            gtgCATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATGCTTAAAAGCGGGTAAGTTAAAGCGAAAGGCATCAGGATTAAAATAGAGATGGCGATAATCGAACAGCGTTTTAAACTCCAg     >  1:10440/1‑250 (MQ=255)                                                                                                                                                                                                                                               cATGATCTGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATGCTTAAAAGCGGGTAAGTTAAAGCGAAAGGCATCAGGATTAAAATAGAGATGGCGATAATCGAACAGCGTTTTAAACTCCAGCgg  <  1:190483/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   TGACCGCTTTGCTTTGCGGGTAAAAATCGTTCCACACTTCGTCGGGGTTAATTTTAATAAACAAGAGCGTGGTTAAAAGTGCGGTAAAAATAAAAGCGATTAAGTAAATATTCAGTTCTAATGTGGCGTACTCCTGCACTTTGAAAATGCTCACGCCCAATGCCACCAAATACACGTCAAACATCACCCAGGGTTTAATATAACTGAGGGAAATCAACAAATTGCGTGGTTTTATGTGCATGATCCGTGACAGCCGCAGCAGAATAACCAGCAGCGCAAAGGCAATGGGCATAAACACGGCGCAAAGAAACACTAAAAATGCAGTGTAAGAAAAACCTTGTGTCGCCATTTTCCACACGCCCAGCCAGACCGAGGCGTCAATTTTTTCGCCTAATAAATCTATGCTTAAAAGCGGGTAAGTTAAAGCGAAAGGCATCAGGATTAAAATAGAGATGGCGATAATCGAACAGCGTTTTAAACTCCAGCGG  >  1/443427‑443914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑