| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 6 | 15,850 | T→G | R217R (AGG→CGG) | unknown ← | Chloride channel protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 6 | 15,850 | 0 | T | G | 95.5% | 51.0 / ‑3.2 | 22 | R217R (AGG→CGG) | unknown | Chloride channel protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (20/1); minor base C (1/0); total (21/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01 | |||||||||||
GTATAAATGGGATGATTGCGCACCCAGCCACGGCATAAATTCGGCAAGACGCCGCCAACGCCTTTTGCCAGCAGGCGGGCGAAAATGCCGCCGAACACCCCGCAAACCACGCCACACAACAAAACCC‑ATAAATACAAATAAGGAATGTCGGTGACGCCGTGGTATTTAGGAAAATACGGG‑TTGTTGCCGTCGATCGCAACCAAAATGAAACCCGCCGCCAATACACCCAGCAGCACCCTCCGCTCCCAACGTAATAATACGCCGCGTCCAAGTTCTTCAATGGCGAAAATCACTCCGGCTAACGGCGCGTTAAATGCTGCCGCCAACCCGCCCGCCGCGCCCGTTGCCATCAGTTCATTGGCACTAAGTCCTTTGAATGCCACGCCGTATTGACGACAAAGATTGCCCCACCATAACATCACCGCCGCGCCCACTTGCACAGA > 6/15612‑16054 | gTATAAATGGGATGATTGCGCACCCAGCCACGGCATAAATTCGGCAAGACGCCGCCAACGCCTTTTGCCAGCAGGCGGGAGAAAATGCCGCCGAACACCCCGCAAACCACGCCACACAACAAAACCC‑ATAAATACAAATAAGGAATGTCGGTGACGCCGTGGTATTTAGGAAAATACGGG‑TTGTTGCCGTCGATCGCAACCAAAATGAAACCCGCCGCCAATACACCCAGCAGCACCcgccgc < 1:217392/243‑1 (MQ=255)gTATAAATGGGATGATTGCGCACCCAGCCACGGCATAAATTCGGCAAGACGCCGCCAACGCCTTTTGCCAGCAGGCGGGAGAAAATGCCGCCGAACACCCCGCAAACCACGCCACACAACAAAACCC‑ATAAATACAAATAAGGAATGTCGGTGACGCCGTGGTATTTAGGAAAATACGGG‑TTGTTGCCGTCGATCGCAACCAAAATGAAACCCGCCGCCAATACACCCAGCAGCACCCGCCGCTCCCAACg > 1:736777/1‑251 (MQ=255)gTATAAATGGGATGATTGCGCACCCAGCCACGGCATAAATTCGGCAAGACGCCGCCAACGCCTTTTGCCAGCAGGCGGGAGAAAATGCCGCCGAACACCCCGCAAACCACGCCACACAACAAAACCC‑ATAAATACAAATAAGGAATGTCGGTGACGCCGTGGTATTTAGGAAAATACGGG‑TTGTTGCCGTCGATCGCAACCAAAATGAAACCCGCCGCCAATACACCCAGCAGCACCCGCCGCTCCCAACg > 1:710482/1‑251 (MQ=255)gTATAAATGGGATGATTGCGCACCCAGCCACGGCATAAATTCGGCAAGACGCCGCCAACGCCTTTTGCCAGCAGGCGGGAGAAAATGCCGCCGAACACCCCGCAAACCACGCCACACAACAAAACCC‑ATAAATACAAATAAGGAATGTCGGTGACGCCGTGGTATTTAGGAAAATACGGG‑TTGTTGCCGTCGATCGCAACCAAAATGAAACCCGCCGCCAATACACCCAGCAGCACCCGCCGCTCCCAACg > 1:659557/1‑251 (MQ=255)gTATAAATGGGATGATTGCGCACCCAGCCACGGCATAAATTCGGCAAGACGCCGCCAACGCCCTTTGCCAGCAGGCGGGAGAAAATGCCGCCGAACACCCCGCAACCCACGCCACACAACAAAACCC‑ATAAATACAACTAAGGAATGTCGGTGACGCCGTGGCATTTAGGAAAATACGGG‑TTGTTGCCGTCGATCGCAACCAAAATGAAACCCGCCGCCAACACACCCAACAGCACCCGCCGCCCCCAACg > 1:136478/1‑251 (MQ=255) ttccccAGCGGGCGGAAGAAAACGCCCCCGACCCCCCCACAACCCACCCCACACAA‑AAAACCCAAAAAAAAAAAAAAAGAAATGTGGGGGACGCCGTGTTATTAAGAAAAAT‑CGGGTTTGTGCCCGCCGACCCCAACAAAAAAAAACCCCCCCGCCAATACCCCCAGCAGCACCCGCCGCTCCCAACGTAATAATACGCCGCGTCCAAGTTCTTCAATGGCGAAAATCACTCCGGCTAACGGCGCGTTaaa < 1:145406/247‑1 (MQ=255) acCCCGCAAACCACGCCACACAACAAAACCC‑ATAAATACAAATAAGGAATGTCGGTGACGCCGTGGTATTTAGGAAAATACGGG‑TTGTTGCCGTCGATCGCAACCAAAATGAAACCCGCCGCCAATACACCCAGCAGCACCCGCCGCTCCCAACGTAATAATACGCCGCGTCCAAGTTCTTCAATGGCGAAAATCACTCCGGCTAACGGCGCGTTAAAtgc > 1:86773/1‑221 (MQ=255) aaCCACGCCACACAACAAAACCC‑ATAAATACAAATAAGGAATGTCGGTGACGCCGTGGTATTTAGGAAAATACGGG‑TTGTTGCCGTCGATCGCAACCAAAATGAAACCCGCCGCCAATACACCCAGCAGCACCCGCCGCTCCCAACGTAATAATACGCCGCGTCCAAGTTCTTCa > 1:111419/1‑175 (MQ=255) caacaaAACCC‑ATAAATACAAATAAGGAATGTCGGTGACGCCGTGGTATTTAGGAAAATACGGG‑TTGCTGCCGTCGATCGCAACCAAAATGAAACCCGCCGCCAATACACCCAGCAGCACCCGCCGCTCCCAACGTAATAATAc > 1:706191/1‑144 (MQ=255) acaaAACCC‑ATAAATACAAATAAGGAATGTCGGTGACGCCGTGGTATTTAGGAAAATACGGG‑TTGTTGCCGTCGATCGCAACCAAAATGAAACCCGCCGCCAATACACCCAGCAGCACCCGCCGCTCCCAACGTAATAATACGCCGCGTCCAAGTTCTTCAATGGCGAAAATCACTCCGGCTAACGGCGCGTTAAAt > 1:816135/1‑197 (MQ=255) c‑ATAAATACAAATAAGGAATGTCGGTGACGCCGTGGCATTTAGGCAAATACGGG‑TTGTTGCCGTCGATCGCAACCAAAATGAAACCCGCCGCCAATACACCCAGCAGCACCCGCCGCTCCCAACGTAATAATACGCCGCGTCCAAGTTCTTCAATGGCGAAAATCACTCCGGCTAACGGCGCGTTAAATGCTGCCGCCAAcccgccc > 1:83699/1‑207 (MQ=255) aTAAATACAAATAAGGAATGTCGGTGACGCCGTGGAATTTAGGAAAATACGGG‑TTGTTGCCGTCGATCGCAACCAAAATGAAACCCGCCGCCAATACACCCAGCAGCACCCGCCGCTCCCAACGTAATAATACGCCGCGTCCAAGTTCTTCAATGGCGAAAATCACTCCGGCTAACGGCGCGTTAAATGCTGCCGCCAACCCGCCCGCCGCGCCCGTTGCCATCAGTTCATTGGCACTAAGTCCTTTGAAt > 1:223447/1‑251 (MQ=255) gTCGGTGACGCCGTGGTATTTAGGAAAATACGGG‑TTGTTGCCGTCGATCGCAACCAAAATGAAACCCGCCGCCAATACACCCAGCAGCACCCGCCGCTCCCAACGTAATAATACGCCGCGTCCAAGTTCTTCAATGGCGAAAATCACTCCGGCTAACGGCGCGTTAAATGCTGCCGCCAACCCGCCCGCCGCGCCCGTTGCCATCAGTTCATTGGCACTAAGTCCTTTGAATGCCACGcc > 1:643475/1‑240 (MQ=255) cGCCGTGGTATTTAGGAAAATACGGG‑TTGTTGCCGTCGATCGCAACCAAAATGAAACCCGCCGCCAATACACCCAGCAGCACCCGCCGCTCCCAACGTAATAATAc > 1:435561/1‑106 (MQ=255) ccGTGGTATTTAGGAAAATACGGG‑TTGTTGCCGTCGATCGCAACCAAAATGAAACCCGCCGCCAATACACCCAGCAGCACCCGCCGCTCCCAACGTAATAATACGCCGCGTCCAAGTTCTTCAATGGCGAAAATCACTCCGGCTAACGGCGCGTTAAATGCTGCCGCCAAcc > 1:768985/1‑172 (MQ=255) gTATTTAGGAAAATACGGG‑TTGTTGCCGTCGATCGCAACCAAAATGAAACCCGCCGCCAATACACCCAGCAGCACCCGCCGCTCCCAACGTAATAATACGCCGCGTCCAAGTTCTTCAATGGCGAAAATCACTCCGGCTAACGGCGCGTTAAATGCTGCCGCCAACCCGCCCGCCGCGCCCGTTGCCATCAGTTCATTgg > 1:52806/1‑200 (MQ=255) gTATTTAGGAAAATACGGG‑TTGTTGCCGTCGATCGCAACCAAAATGAAACCCGCCGCCAATACACCCAGCAGCACCCGCCGCTCCCAACGTAATAATACGCCGCGTCCAAGTTCTTCAATGGCGAAAATCACTCCGGCTAACGGCGCGTTAAATGCTGCCGCCAACCCGCCCGCCGCGCCCGTTGCCATCAGTTCATTGGCACTAAGTCCTTTGAATGCCACGCCGTATTGACGACAAAGATTGCCccac > 1:189277/1‑250 (MQ=255) ggAAAATACGGG‑TTGTTGCCGTCGATCGCAACCAAAATGAAACCCGCCGCCAATACACCCAGCAGCACCCGCCGCTCCCAACGTAATAATACGCCGCGTCCAAGTTCTTCAATGGCGAAAATCACTCCGGCTAACGGCGCGTTAAATGCTGCCGCCAACCCGCCCGCCGCGCCCGTTGCCATCAGTTCATTGGCACTAAGTCCTTTGAATGCCACGCCGTATTGACGACAAAGATTGCCCCACCATAACAt > 1:830310/1‑251 (MQ=255) ggAAAATACGGG‑TTGTTGCCGTCGATCGCAACCAAAATGAAACCCGCCGCCAATACACCCAGCAGCACCCCCCGCTCCCAACGTAATAATACGCCGCGTCCAAGTTCTTCAAt > 1:194120/1‑113 (MQ=255) aaaTACGGG‑TTGTTGCCGTCGATCGCAACCAAAATGAAACCCGCCGCCAATACACCCAGCAGCACCCGCCGCTCCCAACGTAATAATACGCCGCGTCCAAGTTCTTCAATGGCGAAAATCACTCCGGCTAACGGCGCGTTAAATGCTGCCGCCAACCCGCCCGCCGCGCCCGTTGCCATCAGTTCATTGGCACTAAGTCCTTTGAATGCCACGCCGTATTGACGACAAAGATTGCCCCACCATAACATCa > 1:695653/1‑250 (MQ=255) aaTACGGG‑TTGTTGCCGTCGATCGCAACCAAAATGAAACCCGCCGCCAATACACCCAGCAGCACCCGCCGCTCCCAACGTAATAATACGCCGCGTCCAAGTTCTTCAATGGCGAAAATCACTCCGGCTAACGGCGCGTTAAATGCTGCCGCCAACCCGCCCGCCGCGCCCGTTGCCATCAGTTCATTGGCACTAAGTCCTTTGAATGCCACGCCGTATTGACGACAAAGATTGCCCCACCATAACATCAc > 1:322149/1‑250 (MQ=255) aTACGGG‑TTGTTGCCGTCGATCGCAACCAAAATGAAACCCGCCGCCAATACACCCAGCAGCACCCGCCGCTCCCAACGTAATAATAc > 1:398337/1‑87 (MQ=255) aTCGCAACCAAAATGAAACCCGCCGCCAATACACCCAGCAGCACCCGCCGCTCCCAACGTAATAATACGCCGCGTCCAAGTTCTTCAATGGCGAAAATCACTCCGGCTAACGGCGCGTTAAATGCTGCCGCCAACCCGCCCGCCGCGCCCGTTGCCATCAGTTCATTGGCACTAAGTCCTTTGAATGCCACGCCGTATTGACGACAAAGATTGCCCCACCATAACATCACCGCCGCGCCCACTTGCACAGa > 1:744123/1‑251 (MQ=255) | GTATAAATGGGATGATTGCGCACCCAGCCACGGCATAAATTCGGCAAGACGCCGCCAACGCCTTTTGCCAGCAGGCGGGCGAAAATGCCGCCGAACACCCCGCAAACCACGCCACACAACAAAACCC‑ATAAATACAAATAAGGAATGTCGGTGACGCCGTGGTATTTAGGAAAATACGGG‑TTGTTGCCGTCGATCGCAACCAAAATGAAACCCGCCGCCAATACACCCAGCAGCACCCTCCGCTCCCAACGTAATAATACGCCGCGTCCAAGTTCTTCAATGGCGAAAATCACTCCGGCTAACGGCGCGTTAAATGCTGCCGCCAACCCGCCCGCCGCGCCCGTTGCCATCAGTTCATTGGCACTAAGTCCTTTGAATGCCACGCCGTATTGACGACAAAGATTGCCCCACCATAACATCACCGCCGCGCCCACTTGCACAGA > 6/15612‑16054 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |