Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	6	5,527	G→A	E28E (GAG→GAA)	unknown →	probable periplasmic protein NMA1059

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	6	5,527	0	G	A	100.0%	217.4 / NA	75	E28E (GAG→GAA)	unknown	probable periplasmic protein NMA1059
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (42/33);  total (42/33)

GATGCCGATGATGACGAATATGGCGATGACGGTGAATTTTTTGACGAAGATGATGCCTTAGATGATGACGATGAGTTCGTTAAACGTTAAAAAACATTTGTAAAAACAACCGCACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAGCCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGCGGTGCAAATTAAGAAAAATACGCGTAACACACAAGTGCGTTATGACAACAAAGGCAAACAATCCGGTT  >  6/5304‑5765                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               gATGCCGATGATGACGAATATGGCGATGACGGTGAATTTTTTGACGAAGATGATGCCTTAGATGATGACGATGAGTTCGTTAAACGTTAAAAAACATTTGTAAAAACAACCGCACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTaa                                                                                                                                                                                                                      >  1:219922/1‑250 (MQ=255)               cGAATATGGCGATGACGGTGAATTTTTTGACGAAGATGATGCCTTAGATGATGACGATGAGTTCGTTAAACGTTAAAAAACATTTGTAAAAACAACCGCACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAacac                                                                                                                                                                                                                   >  1:489406/1‑239 (MQ=255)               cGAATATGGCGATGACGGTGAATTTTTTGACGAAGATGATGCCTTAGATGATGACGATGAGTTCGTTAAACGTTAAAAAACATTTGTAAAAACAACCGCACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCGCGaa                                                                                                                                                                                                       >  1:324396/1‑251 (MQ=255)                gAATATGGCGATGACGGTGAATTTTTTGACGAAGATGATGCCTTAGATGATGACGATGAGTTCGTTAAACGTTAAAAAACATTTGTAAAAACAACCGCACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGaaa                                                                                                                                                                                                      >  1:788793/1‑251 (MQ=255)                      ggCGATGACGGTGAATTTTTTGACGAAGATGATGCCTTAGATGATGACGATGAGTTCGTTAAACGTTAAAAAACATTTGTAAAAACAACCGCACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTg                                                                                                                                                                                                >  1:291542/1‑251 (MQ=255)                          aTGACGGTGAATTTTTTGACGAAGATGATGCCTTAGATGATGACGATGAGTTCGTTAAACGTTAAAAAACATTTGTAAAAACAACCGCACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGAt                                                                                                                                                                                             <  1:678717/250‑1 (MQ=255)                             aCGGTGAATTTTTTGACGAAGATGATGCCTTAGATGATGACGATGAGTTCGTTAAACGTTAAAAAACATTTGTAAAAACAACCGCACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTg                                                                                                                                                                                                >  1:564012/1‑244 (MQ=255)                              cGGTGAATTTTTTGACGAAGATGATGCCTTAGATGATGACGATGAGTTCGTTAAACGTTAAAAAACATTTGTAAAAACAACCGCACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGa                                                                                                                                                                                        <  1:85369/251‑1 (MQ=255)                                      tttttGACGAAGATGATGCCTTAGATGATGACGATGAGTTCGTTAAACGTTAAAAAACATTTGTAAAAACAACCGCACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATgg                                                                                                                                                                                <  1:224833/251‑1 (MQ=255)                                         ttGACGAAGATGATGCCTTAGATGATGACGATGAGTTCGTTAAACGTTAAAAAACATTTGTAAAAACAACCGCACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAg                                                                                                                                                                             <  1:752864/251‑1 (MQ=255)                                                aGATGATGCCTTAGATGATGACGATGAGTTCGTTAAACGTTAAAAAACATTTGTAAAAACAACCGCACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCAct                                                                                                                                                                        >  1:342890/1‑249 (MQ=255)                                                 gatgatGCCTTAGATGATGACGATGAGTTCGTTAAACGTTAAAAAACATTTGTAAAAACAACCGCACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCtt                                                                                                                                                                     >  1:896734/1‑251 (MQ=255)                                                       gCCTTAGATGATGACGATGAGTTCGTTAAACGTTAAAAAACATTTGTAAAAACAACCGCACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTg                                                                                                                                                               >  1:721770/1‑251 (MQ=255)                                                       gCCTTAGATGATGACGATGAGTTCGTTAAACGTTAAAAAACATTTGTAAAAACAACCGCACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTg                                                                                                                                                               >  1:259490/1‑251 (MQ=255)                                                       gCCTTAGATGATGACGATGAGTTCGTTAAACGTTAAAAAACATTTGTAAAAACAACCGCACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTg                                                                                                                                                               >  1:309161/1‑251 (MQ=255)                                                        ccTTAGATGATGACGATGAGTTCGTTAAACGTTAAAAAACATTTGTAAAAACAACCGCACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGa                                                                                                                                                              >  1:589109/1‑251 (MQ=255)                                                        ccTTAGATGATGACGATGAGTTCGTTAAACGTTAAAAAACATTTGTAAAAACAACCGCACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGa                                                                                                                                                              >  1:542172/1‑251 (MQ=255)                                                            aGATGATGACGATGAGTTCGTTAAACGTTAAAAAACATTTGTAAAAACAACCGCACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGa                                                                                                                                                                                        >  1:656553/1‑221 (MQ=255)                                                                 atgaCGATGAGTTCGTTAAACGTTAAAAAACATTTGTAAAAACAACCGCACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGaa                                                                                                                                                      <  1:658107/250‑1 (MQ=255)                                                                   gaCGATGAGTTCGTTAAACGTTAAAAAACATTTGTAAAAACAACCGCACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAAt                                                                                                                                                   >  1:401179/1‑251 (MQ=255)                                                                                     cgttaaaAAACATTTGTAAAAACAACCGCACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAac                                                                                                                                                                                                                     <  1:328068/167‑1 (MQ=255)                                                                                                   tGTAAAAACAACCGCACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAAAATTAAACCAAGCGATTa                                                                                                                    >  1:567938/1‑250 (MQ=255)                                                                                                       aaaaCAACCGCACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACgag                                                                                                               <  1:321386/251‑1 (MQ=255)                                                                                                        aaaCAACCGCACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACgaga                                                                                                              >  1:293971/1‑251 (MQ=255)                                                                                                           caacCGCACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAg                                                                                                           <  1:686590/251‑1 (MQ=255)                                                                                                                 cACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACg                                                                                                                                                                                                         >  1:45618/1‑151 (MQ=255)                                                                                                                  aCTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAAtgc                                                                                                    <  1:148579/251‑1 (MQ=255)                                                                                                                  aCTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAAtgc                                                                                                    <  1:224607/251‑1 (MQ=255)                                                                                                                   cTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGc                                                                                                                         <  1:165208/229‑1 (MQ=255)                                                                                                                     ttttAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAAtgctg                                                                                                  >  1:79290/1‑250 (MQ=255)                                                                                                                       ttAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCAt                                                                                               <  1:251893/251‑1 (MQ=255)                                                                                                                            ttttATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGAt                                                                                          <  1:291455/251‑1 (MQ=255)                                                                                                                                   ggAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAAc                                                                                                                              <  1:547040/208‑1 (MQ=255)                                                                                                                                   ggAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAAtgc                                                                                                    >  1:398519/1‑234 (MQ=255)                                                                                                                                   ggAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTa                                                                                   >  1:189591/1‑251 (MQ=255)                                                                                                                                      gaAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGAtg                                                                                         <  1:278446/242‑1 (MQ=255)                                                                                                                                          aCCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGaa                                                                                                                                                      >  1:530006/1‑177 (MQ=255)                                                                                                                                             aTGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAg                                                                         <  1:104699/251‑1 (MQ=255)                                                                                                                                                aaaTTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGc                                                                      <  1:511731/251‑1 (MQ=255)                                                                                                                                                  aTTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGCgg                                                                    >  1:332694/1‑251 (MQ=255)                                                                                                                                                  aTTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGCgg                                                                    >  1:286467/1‑251 (MQ=255)                                                                                                                                                         gATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTc                                                                                                                                                                           <  1:729510/141‑1 (MQ=255)                                                                                                                                                         gATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGCGGTGCAAAt                                                             >  1:686850/1‑251 (MQ=255)                                                                                                                                                               gTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGAtg                                                                                         <  1:760255/217‑1 (MQ=255)                                                                                                                                                               gTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGAt                                                                                          <  1:519860/216‑1 (MQ=255)                                                                                                                                                               gTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGCGGTGCAAATTAAGaa                                                       >  1:713005/1‑251 (MQ=255)                                                                                                                                                               gTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGCGGTGCAAATTAAGaa                                                       >  1:794895/1‑251 (MQ=255)                                                                                                                                                                  ttATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGCGGTGCAAATTAAGaaaaa                                                    >  1:462039/1‑251 (MQ=255)                                                                                                                                                                       gctattgcCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGCGGTGCAAATTAAGAAAAATAcg                                                >  1:110076/1‑250 (MQ=255)                                                                                                                                                                             gcCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATgg                                                                                                                                                                                <  1:885754/116‑1 (MQ=255)                                                                                                                                                                             gcCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTc                                                                                                                                                                           >  1:96173/1‑121 (MQ=255)                                                                                                                                                                             gcCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTg                                                                                             <  1:122813/199‑1 (MQ=255)                                                                                                                                                                             gcCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAg                                                                         >  1:1275/1‑219 (MQ=255)                                                                                                                                                                             gcCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGCGGTGCAAATTAAGAAAAATACGCGTAaca                                         >  1:521807/1‑251 (MQ=255)                                                                                                                                                                             gcCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGCGGTGCAAATTAAGAAAAATACGCGTAaca                                         >  1:618288/1‑251 (MQ=255)                                                                                                                                                                              cccTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTc                                                                                                                                                                           <  1:105155/120‑1 (MQ=255)                                                                                                                                                                              cccTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGCGGTGCAAATTAAGAAAAATa                                                  <  1:662404/241‑1 (MQ=255)                                                                                                                                                                              cccTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGCGGTGCAAATTAAGAAAAATACGCGTAacac                                        <  1:522407/251‑1 (MQ=255)                                                                                                                                                                                cTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGCGGTGCAAATTAAGAAAAATACGCGTAacaca                                       >  1:493559/1‑250 (MQ=255)                                                                                                                                                                                 ttAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGCGGTGCAAATTAAGAAAAATACGCGTAAcacaca                                     <  1:623352/251‑1 (MQ=255)                                                                                                                                                                                 ttAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGCGGTGCAAATTAAGAAAAATACGCGTAAcacaca                                     <  1:807141/251‑1 (MQ=255)                                                                                                                                                                                   aaGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTTACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGCGGTGCAAATTAAGAAAAATACGCGTAACACACAAg                                   <  1:451001/251‑1 (MQ=255)                                                                                                                                                                                   aaGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGCGGTGCAAATTAAGAAAAATACGCGTAACACACAAg                                   <  1:90516/251‑1 (MQ=255)                                                                                                                                                                                   aaGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGCGGTGCAAATTAAGAAAAATACGCGTAACACACAAg                                   <  1:843931/251‑1 (MQ=255)                                                                                                                                                                                      cGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGCGGTGCAAATTAAGAAAAATACGCGTAACACACAAGTGc                                <  1:273019/251‑1 (MQ=255)                                                                                                                                                                                         cGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTg                                                                                                                                                               >  1:833363/1‑121 (MQ=255)                                                                                                                                                                                          gCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGCGGTGCAAATTAAGAAAAATACGCGTAACACACAAGTGCGTTa                            >  1:614863/1‑251 (MQ=255)                                                                                                                                                                                           cTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGCGGTGCAAATTAAGAAAAATACGCGTAACACACAAGTGCGTTa                            <  1:763620/250‑1 (MQ=255)                                                                                                                                                                                           cTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGCGGTGCAAATTAAGAAAAATACGCGTAACACACAAGTGCGTTAt                           >  1:545352/1‑251 (MQ=255)                                                                                                                                                                                                        cGCCGAAGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGCGGTGCAAATTAAGAAAAATACGCGTAACACACAAGTGCGTTATGACAACAAAGGCa              >  1:542730/1‑251 (MQ=255)                                                                                                                                                                                                              aGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGc                                                                                                                         >  1:272576/1‑138 (MQ=255)                                                                                                                                                                                                              aGCTAAGGTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCAt                                                                                               >  1:168429/1‑164 (MQ=255)                                                                                                                                                                                                                    ggTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGCGGTGCAAATTAAGAAAAATACGCGTAACACACAAGTGCGTTATGACAACAAAGGCAAACAATCCGGtt  >  1:628694/1‑251 (MQ=255)                                                                                                                                                                                                                    ggTTACCGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGCGGTGCAAATTAAGAAAAATACGCGTAACACACAAGTGCGTTATGACAACAAAGGCAAACAATCCGGtt  >  1:629767/1‑251 (MQ=255)                                                                                                                                                                                                                         ccGACGAACCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGCGGTGCAAATTAAGAAAAATACGCGTAACACACAAGTGCGTTATGa                         <  1:580942/223‑1 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               GATGCCGATGATGACGAATATGGCGATGACGGTGAATTTTTTGACGAAGATGATGCCTTAGATGATGACGATGAGTTCGTTAAACGTTAAAAAACATTTGTAAAAACAACCGCACTTTTTAATTTTTATAGGAGAAAACCATGAAATTCAAAGATTTAGTTTTATTGCTATTGCCCTTAAGCGTCGCTTTAACTGCCAACGCCGAAGCTAAGGTTACCGACGAGCCGGAAAAAAACGTTATTTCTTTTAACACTGAAGTCACGAAAGAAGTGGATTATGACGTAATGGAAGTCACTCTTTTCGTGAAACAGGAAAATAAAAATCTAAAAGAATTGAACCAAGCGATTAACGAGAAAGTGAATGCTGCATTGGATGTGATTAAAAAACAAAGTGCGGTGCAAATTAAGAAAAATACGCGTAACACACAAGTGCGTTATGACAACAAAGGCAAACAATCCGGTT  >  6/5304‑5765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑