Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	5	28,623	C→T	S26N (AGC→AAC)	unknown ←	putative membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	5	28,623	0	C	T	100.0%	210.9 / NA	75	S26N (AGC→AAC)	unknown	putative membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/34);  total (41/34)

ATGCCCACGCGTGCGCAGCTTGGAGACGAAAATCTCATACCCGCCGATAATCACCATCACCAGCAAATTGGCGATCATGACAACATCAATCAGATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGCTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTACGCCTAAAATTCAACGTTTTTTTAACATCATGGGGAAATTGTAAAAACACATTAAAAATCCACCGCACTTTTATTCTTCTAAATTTTCTTTTGTCACCAACAACCGCTCCG  >  5/28388‑28864                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   aTGCCCACGCGTGCGCAGCTTGGAGACGAAAATCTCATACCCGCCGATAATCACCATCACCAGCAAATTGGCGATCATGACAACATCAATCAGATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAAttt                                                                                                                                                                                                                                     >  1:357334/1‑251 (MQ=255)   gCCCACGCGTGCGCAGCTTGGAGACGAAAATCTCATACCCGCCGATAATCACCATCACCAGCAAATTGGCGATCATGACAACATCAATCAGATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTa                                                                                                                                                                                                                                   >  1:149298/1‑251 (MQ=255)                        aaacaaaaatcacacccccGCCGATAATCACCATCACCAGCAAATTGGCGATCATGACAACATCAATCAGATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATaa                                                                                                                                                                                                              <  1:210822/235‑1 (MQ=255)                          acaaaaacctaacccccGCCGATAATCACCATCACCAGCAAATTGGCGATCATGACAACATCAATCAGATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAAcg                                                                                                                                                                                                            <  1:487533/237‑1 (MQ=255)                                     ctccccGCCGATAATCACCATCACCAGCAAATTGGCGATCATGACAACATCAATCAGATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑ttt                                                                                                                                                                                                <  1:859995/248‑1 (MQ=255)                                     acccccGCCGATAATCACCATCACCAGCAAATTGGCGATCATGACAACATCAATCAGATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCAGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑ttt                                                                                                                                                                                                <  1:504329/248‑1 (MQ=255)                                       ccccGCCGATAATCACCATCACCAGCAAATTGGCGATCATGACAACATCAATCAGATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑Tttgt                                                                                                                                                                                              <  1:785665/250‑1 (MQ=255)                                          cgccgATAATCACCATCACCAGCAAATTGGCGATCATGACAACATCAATCAGATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTtgttgt                                                                                                                                                                                           >  1:819137/1‑251 (MQ=255)                                               aTAATCACCATCACCAGCAAATTGGCGATCATGACAACATCAATCAGATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTg                                                                                                                                                                                      >  1:548919/1‑251 (MQ=255)                                                   tcaccatcaccaGCAAATTGGCGATCATGACAACATCAATCAGATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTc                                                                                                                                                                                  <  1:257605/251‑1 (MQ=255)                                                      ccatcaccaGCAAATTGGCGATCATGACAACATCAATCAGATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGt                                                                                                                                                                                    <  1:21383/246‑1 (MQ=255)                                                         tcaccaGCAAATTGGCGATCATGACAACATCAATCAGATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCttgt                                                                                                                                                                            >  1:674590/1‑251 (MQ=255)                                                          caccaGCAAATTGGCGATCATGACAACATCAATCAGATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCCGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCttgtt                                                                                                                                                                           >  1:445895/1‑251 (MQ=255)                                                                       gCGATCATGACAACATCAATCAGATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTccc                                                                                                                                                               >  1:351901/1‑250 (MQ=255)                                                                        cGATCATGACAACATCAATCAGATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTccccc                                                                                                                                                             <  1:546730/251‑1 (MQ=255)                                                                        cGATCATGACAACATCAATCAGATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTccccc                                                                                                                                                             <  1:201259/251‑1 (MQ=255)                                                                          aTCATGACAACATCAATCAGATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑ttt                                                                                                                                                                                                <  1:477883/214‑1 (MQ=255)                                                                          aTCATGACAACATCAATCAGATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCaa                                                                                                                                                           >  1:829099/1‑251 (MQ=255)                                                                           tCATGACAACATCAATCAGATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCAt                                                                                                                                                                                                                >  1:368533/1‑198 (MQ=255)                                                                            cATGACAACATCAATCAGATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑ttt                                                                                                                                                                                                >  1:130442/1‑212 (MQ=255)                                                                              tGACAACATCAATCAGATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAATTTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCaataa                                                                                                                                                        <  1:896095/251‑1 (MQ=255)                                                                                   acaTCAATCAGATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATaa                                                                                                                                                                                                              <  1:148591/192‑1 (MQ=255)                                                                                   acaTCAATCAGATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCaataaaaaata                                                                                                                                                  >  1:124937/1‑251 (MQ=255)                                                                                    caTCAATCAGATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCaataaaaaataa                                                                                                                                                 >  1:314455/1‑251 (MQ=255)                                                                                       caatcaGATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑ttt                                                                                                                                                                                                >  1:240015/1‑201 (MQ=255)                                                                                             gATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑ttt                                                                                                                                                                                                <  1:810852/195‑1 (MQ=255)                                                                                             gATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑tt                                                                                                                                                                                                 <  1:551294/194‑1 (MQ=255)                                                                                             gATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATaaa                                                                                                                                        >  1:431764/1‑251 (MQ=255)                                                                                              aTTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCaataaaaaat                                                                                                                                                   <  1:236541/239‑1 (MQ=255)                                                                                                     aCCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGt                                                                                                                                                                                    >  1:695274/1‑199 (MQ=255)                                                                                                        gCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGaaaaa                                                                                                                             >  1:499230/1‑251 (MQ=255)                                                                                                            aCATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTccccc                                                                                                                                                             >  1:871598/1‑215 (MQ=255)                                                                                                               tCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTg                                                                                                                      <  1:395040/251‑1 (MQ=255)                                                                                                               tCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTg                                                                                                                      <  1:711706/251‑1 (MQ=255)                                                                                                                  cGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACgg                                                                                                                                                                     <  1:453698/201‑1 (MQ=255)                                                                                                                    gtgtTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑ttt                                                                                                                                                                                                <  1:847831/172‑1 (MQ=255)                                                                                                                    gtgtTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑ttt                                                                                                                                                                                                <  1:157330/172‑1 (MQ=255)                                                                                                                    gtgtTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑Tttgttg                                                                                                                                                                                            >  1:799219/1‑176 (MQ=255)                                                                                                                    gtgtTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTccccc                                                                                                                                                             <  1:296518/207‑1 (MQ=255)                                                                                                                    gtgtTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCaataa                                                                                                                                                        <  1:134562/212‑1 (MQ=255)                                                                                                                    gtgtTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTa                                                                                                                 >  1:125591/1‑251 (MQ=255)                                                                                                                    gtgtTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTa                                                                                                                 >  1:731465/1‑251 (MQ=255)                                                                                                                       ttGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTACGc                                                                                                              <  1:167388/251‑1 (MQ=255)                                                                                                                         ggAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTACGc                                                                                                              >  1:216782/1‑249 (MQ=255)                                                                                                                            aTCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTACGCCTaaa                                                                                                         >  1:280169/1‑251 (MQ=255)                                                                                                                             tCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑ttt                                                                                                                                                                                                <  1:675902/163‑1 (MQ=255)                                                                                                                                    ccTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGt                                                                                                                                                                                    >  1:60170/1‑168 (MQ=255)                                                                                                                                       gTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGtt                                                                                                                                                                                   >  1:560936/1‑166 (MQ=255)                                                                                                                                                cAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGAATGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAgg                                                                                                                                     <  1:558746/203‑1 (MQ=255)                                                                                                                                                  aTTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTACGCCTAAAATTCAACGTTTTTTTAAcatca                                                                                   >  1:356076/1‑251 (MQ=255)                                                                                                                                                          ccACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTACGCCTAAAATTCAACGTTTTTTTAACATCATGGGGaa                                                                            <  1:795093/250‑1 (MQ=255)                                                                                                                                                          ccACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGATGCAGCCAACGGTTGGCGAAAATAATTTTACTGAATGCCGCCGGCTCATAACGAGGTTTAA‑TTTGTCGTCCTTGGTTCGCTTGTTGCACGGttttcctc                                                                                                                                                             >  1:64972/1‑164 (MQ=255)                                                                                                                                                               gggATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGCTTCCCCCAATAAAAAATAAAAg                                                                                                                                              >  1:19501/1‑179 (MQ=255)                                                                                                                                                                ggATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTACGCCTAAAATTCAACGTTTTTTTAACATCATGGGGAAATTg                                                                        <  1:601674/248‑1 (MQ=255)                                                                                                                                                                    ttCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTACGCCTAAAATTCAACGTTTTTTTAACATCATGGGGAAATTGTAAAAac                                                                 <  1:245434/251‑1 (MQ=255)                                                                                                                                                                           aTTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTACGCCTAAAATTCAACGTTTTTTTAACATCATGGGGAAATTGTAAAAACACATTaa                                                          >  1:497668/1‑251 (MQ=255)                                                                                                                                                                            tttATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑ttt                                                                                                                                                                                                <  1:49578/116‑1 (MQ=255)                                                                                                                                                                                    cATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTACGc                                                                                                              >  1:709360/1‑190 (MQ=255)                                                                                                                                                                                    cATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTACGCCTAAAATTCAACGTTTTTTTAACATCATGGGGAAATTGTAAAAACACATTAAAAATCCAc                                                  >  1:392800/1‑250 (MQ=255)                                                                                                                                                                                    cATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTACGCCTAAAATTCAACGTTTTTTTAACATCATGGGGAAATTGTAAAAACACATTAAAAATCCAc                                                  >  1:850079/1‑250 (MQ=255)                                                                                                                                                                                      tAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTg                                                                                                                      >  1:608202/1‑180 (MQ=255)                                                                                                                                                                                          tGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTACGCCTAAAATTCAACGTTTTTTTAACATCATGGGGAAATTGTAAAAACACATTAAAAATCCACCGCACtt                                           >  1:492419/1‑251 (MQ=255)                                                                                                                                                                                              ttGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTACGCCTAAAATTCAACGTTTTTTTAACATCATGGGGAAATTGTAAAAACACATTAAAAATCCACCGCACTTTTAt                                       <  1:112464/251‑1 (MQ=255)                                                                                                                                                                                               tGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTACGCCTAAAATTCAACGTTTTTTTAACATCATGGGGAAATTGTAAAAACACATTAAAAATCCACCGCACTTTTAtt                                      <  1:337433/251‑1 (MQ=255)                                                                                                                                                                                                     aTAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTACGCCTAAAATTCAACGTTTTTTTAACATCATGGGGAAATTGTAAAAACACATTAAAAATCCACCGCACTTTTATTCTTCTa                                >  1:582127/1‑251 (MQ=255)                                                                                                                                                                                                     aTAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTACGCCTAAAATTCAACGTTTTTTTAACATCATGGGGAAATTGTAAAAACACATTAAAAATCCACCGCACTTTTATTCTTCTa                                >  1:576803/1‑251 (MQ=255)                                                                                                                                                                                                       aaTCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATCAACTGTTTTACGCCTAAAATTCAACGTTTTTTTAACATCATGGGGAAATTGTAAAAACACATTaaaaa                                                       <  1:738583/226‑1 (MQ=255)                                                                                                                                                                                                               cGAGATAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTACGCCTAAAATTCAACGTTTTTTTAACATCATGGGGAAATTGTAAAAACACATTAAAAATCCACCGCACTTTTATTCTTCTAAATTTTCttt                      <  1:362229/251‑1 (MQ=255)                                                                                                                                                                                                                gagaTAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTACGCCTAAAATTCAACGTTTTTTTAACATCATGGGGAAATTGTAAAAACACATTAAAAATCCACCGCACTTTTATTCTTCTAAATTTTCtttt                     >  1:93425/1‑251 (MQ=255)                                                                                                                                                                                                                 agaTAAATCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTACGc                                                                                                              >  1:827476/1‑161 (MQ=255)                                                                                                                                                                                                                      aaTCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTACGCCTAAAATTCAACGTTTTTTTAACATCATGGGGAAATTGTAAAAACACATTAAAAATCCACCGCACTTTTAttc                                     <  1:545537/229‑1 (MQ=255)                                                                                                                                                                                                                      aaTCGGCAGTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTACGCCTAAAATTCAACGTTTTTTTAACATCATGGGGAAATTGTAAAAACACATTAAAAATCCACCGCACTTTTAttc                                     >  1:297412/1‑229 (MQ=255)                                                                                                                                                                                                                              gTTGCAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTACGCCTAAAATTCAACGTTTTTTTAACATCATGGGGAAATTGTAAAAACACATTAAAAATCCACCGCACTTTTATTCTTCTAAATTTTCTTTTGTCACCAACAACCg       >  1:707951/1‑251 (MQ=255)                                                                                                                                                                                                                                  cAGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTACGCCTAAAATTCAACGTTTTTTTAACATCATGGGGAAATTGTAAAAACACATTAAAAATCCACCGCACTTTTATTCTTCTAAATTTTCTTTTGTCACCAACAACCGCTc    >  1:105002/1‑250 (MQ=255)                                                                                                                                                                                                                                   aGCCAACGGTTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTACGCCTAAAATTCAACGTTTTTTTAACATCATGGGGAAATTGTAAAAACACATTAAAAATCCACCGCACTTTTATTCTTCTAAATTTTCTTTTGTCACCAACAACCGCTCCg  >  1:244714/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   ATGCCCACGCGTGCGCAGCTTGGAGACGAAAATCTCATACCCGCCGATAATCACCATCACCAGCAAATTGGCGATCATGACAACATCAATCAGATTCAATACCGCCAACATCACGGTGTTGGAATCCATTTCCTGTAAATTGACAATTAAATGCCACAGGGATTTCATAAATTTATAGGCATAAATGCCTTGCACGATAATCAACCCGAGATAAATCGGCAGTTGCAGCCAACGGCTGGCGAAAATAATTTTACTGATTGCCGCCGGCTCATAACGCGGTTTAA‑TTTGTTGTCCTTGGTTCGCTTGTTGCACGGTTTCCCCCAATAAAAAATAAAAGTATAAAAGGCGGAAAAATTAACTGTTTTACGCCTAAAATTCAACGTTTTTTTAACATCATGGGGAAATTGTAAAAACACATTAAAAATCCACCGCACTTTTATTCTTCTAAATTTTCTTTTGTCACCAACAACCGCTCCG  >  5/28388‑28864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑