Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	5	24,288	C→T	G366D (GGC→GAC)	unknown ←	TonB‑dependent receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	5	24,288	0	C	T	100.0%	157.8 / NA	56	G366D (GGC→GAC)	unknown	TonB‑dependent receptor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/32);  total (24/32)

TCATATAGTCCGCTTTTTTGCGAAGGACCGTCGTAAAAAGGACTGAGTTCTTCCGGGAAACGATTTTTAGTATATTGATTTTTAAATAAATTCACGCCTAAGGTCGTTTTTAGATCGGTTTCTTTTGGCAGCGAAAAGGTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGCCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATGATTATCAAGCGTTCGGTATTGTAAATTTAAGGTATGTCGATCATCCGAAAATTCCACTTTCACTAAATGGCTTTTTGAACGCTGAACCAAACTTGAAGGATCTATCGGTGTTAAATCCCATTGCGGCACTTCGTTTT  >  5/24045‑24528                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 tCATATAGTCCGCTTTTTTGCGAAGGACCGTCGTAAAAAGGACTGAGTTCTTCCGGGAAACGATTTTTAGTATATTGATTTTTAAATAAATTCACGCCTAAGGTCGTTTTTAGATCGGTTTCTTTTGGCAGCGAAAAGGTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGt                                                                                                                                                                                                                                            <  1:158824/250‑1 (MQ=255)      taGTCCGCTTTTTTGCGAAGGACCGTCGTAAAAAGGACTGAGTTCTTCCGGGAAACGATTTTTAGTATATTGATTTTTAAATAAATTCACGCCTAAGGTCGTTTTTAGATCGGTTTCTTTTGGCAGCGAAAAGGTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATgcg                                                                                                                                                                                                                                      <  1:726231/251‑1 (MQ=255)                  ttGCGAAGGACCGTCGTAAAAAGGACTGAGTTCTTCCGGGAAACGATTTTTAGTATATTGATTTTTAAATAAATTCACGCCTAAGGTCGTTTTTAGATCGGTTTCTTTTGGCAGCGAAAAGGTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACAt                                                                                                                                                                                                                          <  1:183239/251‑1 (MQ=255)                  ttGCGAAGGACCGTCGTAAAAAGGACTGAGTTCTTCCGGGAAACGATTTTTAGTATATTGATTTTTAAATAAATTCACGCCTAAGGTCGTTTTTAGATCGGTTTCTTTTGGCAGCGAAAAGGTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACAt                                                                                                                                                                                                                          <  1:520927/251‑1 (MQ=255)                  ttGCGAAGGACCGTCGTAAAAAGGACTGAGTTCTTCCGGGAAACGATTTTTAGTATATTGATTTTTAAATAAATTCACGCCTAAGGTCGTTTTTAGATCGGTTTCTTTTGGCAGCGAAAAGGTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACAt                                                                                                                                                                                                                          <  1:73620/251‑1 (MQ=255)                   tGCGAAGGACCGTCGTAAAAAGGACTGAGTTCTTCCGGGAAACGATTTTTAGTATATTGATTTTTAAATAAATTCACGCCTAAGGTCGTTTTTAGATCGGTTTCTTTTGGCAGCGAAAAGGTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACAtt                                                                                                                                                                                                                         <  1:260335/251‑1 (MQ=255)                       aaGGACCGTCGTAAAAAGGACTGAGTTCTTCCGGGAAACGATTTTTAGTATATTGATTTTTAAATAAATTCACGCCTAAGGTCGTTTTTAGATCGGTTTCTTTTGGCAGCGAAAAGGTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTgaga                                                                                                                                                                                                                     <  1:60642/251‑1 (MQ=255)                       aaGGACCGTCGTAAAAAGGACTGAGTTCTTCCGGGAAACGATTTTTAGTATATTGATTTTTAAATAAATTCACGCCTAAGGTCGTTTTTAGATCGGTTTCTTTTGGCAGCGAAAAGGTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTgaga                                                                                                                                                                                                                     <  1:416787/251‑1 (MQ=255)                         ggACCGTCGTAAAAAGGACTGAGTTCTTCCGGGAAACGATTTTTAGTATATTGATTTTTAAATAAATTCACGCCTAAGGTCGTTTTTAGATCGGTTTCTTTTGGCAGCGAAAAGGTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGAt                                                                                                                                                                                                                    >  1:680010/1‑250 (MQ=255)                                   aaaaaGGACTGAGTTCTTCCGGGAAACGATTTTTAGTATATTGATTTTTAAATAAATTCACGCCTAAGGTCGTTTTTAGATCGGTTTCTTTTGGCAGCGAAAAGGTATAACTGTTTCTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTgaga                                                                                                                                                                                                                     <  1:225985/239‑1 (MQ=255)                                   aaaaaGGACTGAGTTCTTCCGGGAAACGATTTTTAGTATATTGATTTTTAAATAAATTCACGCCTAAGGTCGTTTTTAGATCGGTTTCTTTTGGCAGCGAAAAGGTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCa                                                                                                                                                                                                         <  1:37241/251‑1 (MQ=255)                                   aaaaaGGACTGAGTTCTTCCGGGAAACGATTTTTAGTATATTGATTTTTAAATAAATTCACGCCTAAGGTCGTTTTTAGATCGGTTTCTTTTGGCAGCGAAAAGGTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCa                                                                                                                                                                                                         <  1:839461/251‑1 (MQ=255)                                         gACTGAGTTCTTCCGGGAAACGATTTTTAGTATATTGATTTTTAAATAAATTCACGCCTAAGGTCGTTTTTAGATCGGTTTCTTTTGGCAGCGAAAAGGTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCattat                                                                                                                                                                                                     <  1:555523/249‑1 (MQ=255)                                                  cttcCGGGAAACGATTTTTAGTATATTGATTTTTAAATAAATTCACGCCTAAGGTCGTTTTTAGATCGGTTTCTTTTGGCAGCGAAAAGGTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTg                                                                                                                                                                                          >  1:597144/1‑251 (MQ=255)                                                  cttcCGGGAAACGATTTTTAGTATATTGATTTTTAAATAAATTCACGCCTAAGGTCGTTTTTAGATCGGTTTCTTTTGGCAGCGAAAAGGTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTg                                                                                                                                                                                          >  1:459886/1‑251 (MQ=255)                                                                   ttAGTATATTGATTTTTAAATAAATTCACGCCTAAGGTCGTTTTTAGATCGGTTTCTTTTGGCAGCGAAAAGGTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAAtt                                                                                                                                                                         >  1:718482/1‑251 (MQ=255)                                                                     aGTATATTGATTTTTAAATAAATTCACGCCTAAGGTCGTTTTTAGATCGGTTTCTTTTGGCAGCGAAAAGGTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAAtt                                                                                                                                                                         >  1:839906/1‑249 (MQ=255)                                                                        atatTGATTTTTAAATAAATTCACGCCTAAGGTCGTTTTTAGATCGGTTTCTTTTGGCAGCGAAAAGGTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCAttattatta                                                                                                                                                                                                <  1:239894/223‑1 (MQ=255)                                                                              aTTTTTAAATAAATTCACGCCTAAGGTCGTTTTTAGATCGGTTTCTTTTGGCAGCGAAAAGGTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCaa                                                                                                                                                              >  1:767336/1‑251 (MQ=255)                                                                                          atTCACGCCTAAGGTCGTTTTTAGATCGGTTTCTTTTGGCAGCGAAAAGGTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTgaga                                                                                                                                                                                                                     <  1:321317/184‑1 (MQ=255)                                                                                               cgcgtAAGGTCGTTTTTAGATCGGTTTCTTTTGGCAGCGAAAAGGTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGata                                                                                                                                             >  1:650115/5‑251 (MQ=255)                                                                                               cGCCTAAGGTCGTTTTTAGATCGGTTTCTTTTGGCAGCGAAAAGGTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATAc                                                                                                                                                                                                                            <  1:348925/172‑1 (MQ=255)                                                                                                  cTAAGGTCGTTTTTAGATCGGTTTCTTTTGGCAGCGAAAAGGTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGTCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATGa                                                                                                                                          <  1:876913/251‑1 (MQ=255)                                                                                                                  aTCGGTTTCTTTTGGCAGCGAAAAGGTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATGATTATCAAGCGTTCGGt                                                                                                                          <  1:395931/251‑1 (MQ=255)                                                                                                                                    cGAAAAGGTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATGATTATCAAGCGTTCGGTATTGTAAATTTAAGGt                                                                                                          <  1:866537/249‑1 (MQ=255)                                                                                                                                          ggTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAAtt                                                                                                                                                                                     >  1:295736/1‑168 (MQ=255)                                                                                                                                          ggTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATg                                                                                                                                           >  1:22995/1‑210 (MQ=255)                                                                                                                                                 cTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACAt                                                                                                                                                                                                                          >  1:3613/1‑124 (MQ=255)                                                                                                                                                    ttACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATGATTATCAAGCGTTCGGTATTGTAAATTTAAGGTATGTCGATCATCCGaaa                                                                                         <  1:308158/250‑1 (MQ=255)                                                                                                                                                        tAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATGATTATCAAGCGTTCGGTATTGTAAATTTAAGGTATGTCGATCATCCGAAAATTc                                                                                     >  1:647358/1‑250 (MQ=255)                                                                                                                                                         aaTATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGAc                                                                                                                                                     <  1:460260/185‑1 (MQ=255)                                                                                                                                                         aaTATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATGATTATCAAGCGTTCGGTATTGTAAATTTAAGGTATGTCGATCATCCGAAAATTCCa                                                                                   >  1:276314/1‑251 (MQ=255)                                                                                                                                                                 caaTATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCa                                                                                                                                                                                                         <  1:506303/125‑1 (MQ=255)                                                                                                                                                                  aaTATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATGATTATCAAGCGTTCGGTATTGTAAATTTAAGGTATGTCGATCATCCGAAAATTCCACTTTCACTa                                                                          <  1:400812/251‑1 (MQ=255)                                                                                                                                                                        cGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGc                                                                                                                                                                                                           <  1:195359/116‑1 (MQ=255)                                                                                                                                                                            gTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGtt                                                                                                                                                                                                                                           <  1:10901/80‑1 (MQ=255)                                                                                                                                                                            gTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATGATTATCAAGCGTTCGGTATTGTAAATTTAAGGTATGTCGat                                                                                                  <  1:325051/217‑1 (MQ=255)                                                                                                                                                                            gTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATGATTATCAAGCGTTCGGTATTGTAAATTTAAGGTATGTCGATCATCCGAAAATTCCACTTTCACTAAATGGCtttt                                                                >  1:663174/1‑251 (MQ=255)                                                                                                                                                                                                tttCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATGATTATCAAGCGTTCGGTATTGTAAATTTaa                                                                                                             >  1:209124/1‑186 (MQ=255)                                                                                                                                                                                                tttCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATGATTATCAAGCGTTCGGTATTGTAAATTTAAGGTATGTCGa                                                                                                   >  1:255849/1‑196 (MQ=255)                                                                                                                                                                                                tttCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATGATTATCAAGCGTTCGGTATTGTAAATTTAAGGTATGTCGATCATCCGAAAATTCCACTTTCACTAAATGGCTTTTTGAACGCTGAACCAAACTTg                                            >  1:456685/1‑251 (MQ=255)                                                                                                                                                                                                tttCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATGATTATCAAGCGTTCGGTATTGTAAATTTAAGGTATGTCGATCATCCGAAAATTCCACTTTCACTAAATGGCTTTTTGAACGCTGAACCAAACTTg                                            >  1:823174/1‑251 (MQ=255)                                                                                                                                                                                                tttCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGATTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATGATTATCAAGCGTTCGGTATTGTAAATTTAAGGTATGTCGATCATCCGAAAATTCCACTTTCACTAAATGGCTTTTTGAACGCTGAACCAAACTTg                                            >  1:591305/1‑251 (MQ=255)                                                                                                                                                                                                   cAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTAAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATGATTATCAAGCGTTCGGTATTGTAAATTTAAGGTATGTCGATCATCCGAAAATTCCACTTTCACTAAATGGCTTTTTGAACGCTGAACCAAACTTGAAg                                         >  1:322227/1‑251 (MQ=255)                                                                                                                                                                                                     aTACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATGATTATCAAGCGTTCGGTATTGTAAATTTAAGGTATGTCGATCATCCGAAAATTCCACTTTCACTAAATGGCTTTTTGAACGCTGAACCAAACTTGAAGGa                                       <  1:292933/251‑1 (MQ=255)                                                                                                                                                                                                     aTACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATGATTATCAAGCGTTCGGTATTGTAAATTTAAGGTATGTCGATCATCCGAAAATTCCACTTTCACTAAATGGCTTTTTGAACGCTGAACCAAACTTGAAGGa                                       <  1:722423/251‑1 (MQ=255)                                                                                                                                                                                                        ccTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTg                                                                                                                                                                                                                        <  1:109012/71‑1 (MQ=255)                                                                                                                                                                                                                ccgccAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATGATTATCAAGCGTTCGGTATTGTAAATTTAAGGTATGTCGATCATCCGAAAATTCCACTTTCACTAAATGGCTTTTTGAACGCTGAACCAAACTTGAAGGATCTATCgg                               <  1:862079/248‑1 (MQ=255)                                                                                                                                                                                                                  gccAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATAc                                                                                                                                                                                                                            >  1:412711/1‑57 (MQ=255)                                                                                                                                                                                                                  gccAAAACGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATGATTATCAAGCGTTCGGTATTGTAAATTTAAGGTATGTCGATCATCCGAAAATTCCACTTTCACTAAATGGCTTTTTGAACGCTGAACCAAACTTGAAGGATCTATCgg                               <  1:146616/246‑1 (MQ=255)                                                                                                                                                                                                                      aaaCGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATGATTATCAAGCGTTCGGTATTGTAAATTTAAGGTATGTCGATCATCCGAAAATTCCACTTTCACTAAATGGCTTTTTGAACGCTGAACCAAACTTGAAGGATCTATCGGTGTTAAATc                      <  1:324933/251‑1 (MQ=255)                                                                                                                                                                                                                       aaCGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATGATTATCAAGCGTTCGGTATTGTAAATTTAAGGTATGTCGATCATCCGAAAATTCCACTTTCACTAAATGGCTTTTTGAACGCTGAACCAAACTTGAAGGATCTATCGGTGTTAAATc                      >  1:633874/1‑250 (MQ=255)                                                                                                                                                                                                                       aaCGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATGATTATCAAGCGTTCGGTATTGTAAATTTAAGGTATGTCGATCATCCGAAAATTCCACTTTCACTAAATGGCTTTTTGAACGCTGAACCAAACTTGAAGGATCTATCGGTGTTAAATc                      >  1:363673/1‑250 (MQ=255)                                                                                                                                                                                                                        aCGTGAACCGCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATGATTATCAAGCGTTCGGTATTGTAAATTTAAGGTATGTCGATCATCCGAAAATTCCACTTTCACt                                                                           <  1:275957/196‑1 (MQ=255)                                                                                                                                                                                                                                 gCTCGGATACTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATGATTATCAAg                                                                                                                                  >  1:238702/1‑132 (MQ=255)                                                                                                                                                                                                                                          cTTTTGTTTGTCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATGATTATCAAGCGTTCGGTATTGTAAATTTAAGGTATGTCGATCATCCGAAAATTCCACTTTCACTAAATGGCTTTTTGAACGCTGAACCAAACTTGAAGGATCTATCGGTGTTAAATCCCATTGCGGCACTTCGtttt  >  1:292723/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 TCATATAGTCCGCTTTTTTGCGAAGGACCGTCGTAAAAAGGACTGAGTTCTTCCGGGAAACGATTTTTAGTATATTGATTTTTAAATAAATTCACGCCTAAGGTCGTTTTTAGATCGGTTTCTTTTGGCAGCGAAAAGGTATAACTGTTACTAATATCAACAATATCCGCTGTATTTTTGGTTTCCAAATATTTCAATACCTGCCAGCCGCCAAAACGTGAACCGCTCGGATACTTTTGTTTGCCTACGTTATGCGCCAGCAATACATTGAGATCAAGATAGCCATTATTATTAAAATTGTAATTTAACTGATAATTCTGATTCTCAATTTTTCGACTACCGATATGATTATCAAGCGTTCGGTATTGTAAATTTAAGGTATGTCGATCATCCGAAAATTCCACTTTCACTAAATGGCTTTTTGAACGCTGAACCAAACTTGAAGGATCTATCGGTGTTAAATCCCATTGCGGCACTTCGTTTT  >  5/24045‑24528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑