Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	4	13,057	C→T	E146K (GAG→AAG)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	4	13,057	0	C	T	100.0%	121.5 / NA	46	E146K (GAG→AAG)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/25);  total (21/25)

CTCTTTAAGCAACATTTTTCCTAAATGAGTTATATATCTAGAACCTTTAGGTTTTCCTGATGATTTATCATAAATAGGCATATTCGTATTTCTATCTATATTAAATTTAGTTTTTATTAAACCTAATCTTTCTAAATGCTCTTTATAGCTCTCTTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTCATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCAAATAATCTCTTCATCATTTAAGTCTGATAGTAAATTGAGTAAATGGATAGATTGAGATATTTGTACTTGTTCTTTATTTAATCCATTCGCCACTATAGAGGCAATATGCTCAAGACGCTTTTCACTAGCAGCTCGAAAAGCGTAGAATAAACCATCTTCAAACAATGCTATATATTTAGATAATTTTTCAGCTTCTGAAC  >  4/12812‑13296                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                ctctTTAAGCAACATTTTTCCTAAATGAGTTATATATCTAGAACCTTTAGGTTTTCCTGATGATTTATCATAAATAGGCATATTCGTATTTCTATCTATATTAAATTTAGTTTTTATTAAACCTAATCTTTCTAGATGCTCTTTATAGCTCTCTTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATcac                                                                                                                                                                                                                                            <  1:160719/251‑1 (MQ=255)      tAAGCAACATTTTTCCTAAATGAGTTATATATCTAGAACCTTTAGGTTTTCCTGATGATTTATCATAAATAGGCATATTCGTATTTCTATCTATATTAAATTTAGTTTTTATTAAACCTAATCTTTCTAAATGCTCTTTATAGCTCTCTTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCAccc                                                                                                                                                                                                                                       <  1:150866/251‑1 (MQ=255)                             gTTATATATCTAGAACCTTTAGGTTTTCCTGATGATTTATCATAAATAGGCATATTCGTATTTCTATCTATATTAAATTTAGTTTTTATTAAACCTAATCTTTCTAAATGCTCTTTATAGCTCTCTTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAAt                                                                                                                                                                                                                  >  1:184891/1‑249 (MQ=255)                                    atCTAGAACCTTTAGGTTTTCCTGATGATTTATCATAAATAGGCATATTCGTATTTCTATCTATATTAAATTTAGTTTTTATTAAACCTAATCTTTCTAAATGCTCTTTATAGCTCTCTTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGgtg                                                                                                                                                                                                                            >  1:576795/1‑232 (MQ=255)                                    atCTAGAACCTTTAGGTTTTCCTGATGATTTATCATAAATAGGCATATTCGTATTTCTATCTATATTAAATTTAGTTTTTATTAAACCTAATCTTTCTAAATGCTCTTTATAGCTCTCTTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCaa                                                                                                                                                                                                         >  1:650816/1‑251 (MQ=255)                                                                       tAAATAGGCATATTCGTATTTCTATCTATATTAAATTTAGTTTTTATTAAACCTAATCTTTCTAAATGCTCTTTATAGCTCTCTTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCAccc                                                                                                                                                                                                                                       >  1:598373/1‑186 (MQ=255)                                                                                    tCGTATTTCTATCTATATTAAATTTAGTTTTTATTAAACCTAATCTTTCTAAATGCTCTTTATAGCTCTCTTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCttatc                                                                                                                                                                                                                                              >  1:628420/1‑166 (MQ=255)                                                                                        aTTTCTATCTATATTAAATTTAGTTTTTATTAAACCTAATCTTTCTAAATGCTCTTTATAGCTCTCTTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGgtg                                                                                                                                                                                                                            <  1:582497/180‑1 (MQ=255)                                                                                                  atatTAAATTTAGTTTTTATTAAACCTAATCTTTCTAAATGCTCTTTATAGCTCTCTTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTg                                                                                                                                                                                                                                >  1:781147/1‑166 (MQ=255)                                                                                                  atatTAAATTTAGTTTTTATTAAACCTAATCTTTCTAAATGCTCTTTATAGCTCTCTTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCAAATAAtct                                                                                                                                                                                                  <  1:879332/196‑1 (MQ=255)                                                                                                                       aaaCCTAATCTTTCTAAATGCTCTTTATAGCTCTCTTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCAAATAATCTCTTCATCATTTAAGTCTGATAGTAAATTGAGTAAATGGATAGATTGAGATATTTGTACTTGTTCTTTATTTAATcc                                                                                                                      <  1:256094/251‑1 (MQ=255)                                                                                                                        aaCCTAATCTTTCTAAATGCTCTTTATAGCTCTCTTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAgtg                                                                                                                                                                                                                                   <  1:111409/141‑1 (MQ=255)                                                                                                                        aaCCTAATCTTTCTAAATGCTCTTTATAGCTCTCTTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAATc                                                                                                                                                                                                                 <  1:444975/159‑1 (MQ=255)                                                                                                                         aCCTAATCTTTCTAAATGCTCTTTATAGCTCTCTTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCAccc                                                                                                                                                                                                                                       <  1:339072/136‑1 (MQ=255)                                                                                                                         aCCTAATCTTTCTAAATGCTCTTTATAGCTCTCTTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGgtg                                                                                                                                                                                                                            <  1:71797/147‑1 (MQ=255)                                                                                                                                  ttCTAAATGCTCTTTATAGCTCTCTTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAgtgt                                                                                                                                                                                                                                  <  1:155748/132‑1 (MQ=255)                                                                                                                                   tCTAAATGCTCTTTATAGCTCTCTTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGgtg                                                                                                                                                                                                                            >  1:52715/1‑137 (MQ=255)                                                                                                                                             ctTTATAGCTCTCTTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGtt                                                                                                                                                                                                                                 >  1:428823/1‑122 (MQ=255)                                                                                                                                             ctTTATAGCTCTCTTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGgtg                                                                                                                                                                                                                            >  1:378025/1‑127 (MQ=255)                                                                                                                                             ctTTATAGCTCTCTTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCAAATAATCTCTTCATCATTTAAGTCTGATAGTAAATTGAGTAAATGGATAGATTGAGata                                                                                                                                               <  1:64594/204‑1 (MQ=255)                                                                                                                                             ctTTATAGCTCTCTTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCAAATAATCTCTTCATCATTTAAGTCTGATAGTAAATTGAGTAAATGGATAGATTGAGATATTTGTACTTGTTCTTTATTTAATCCATTCGCCACTATAGAGGCAata                                                                                                >  1:274937/1‑251 (MQ=255)                                                                                                                                               ttATAGCTCTCTTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATcac                                                                                                                                                                                                                                            >  1:718215/1‑109 (MQ=255)                                                                                                                                                    gCTCTCTTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCAAATAATCTCTTCATCATTTAAGTCTGATAGTAAATTGAGTAAATGGATAGATTGAGat                                                                                                                                                <  1:60108/196‑1 (MQ=255)                                                                                                                                                       ctctTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCGATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCAAATAATCTCTTCATCATTTAAGTCTGATAGTAAATTGAGTAAATg                                                                                                                                                             <  1:56825/180‑1 (MQ=255)                                                                                                                                                       ctctTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAgtgt                                                                                                                                                                                                                                  >  1:590831/1‑111 (MQ=255)                                                                                                                                                         ctTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGgtg                                                                                                                                                                                                                            >  1:170502/1‑115 (MQ=255)                                                                                                                                                                       ttattaGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGGTGtaaata                                                                                                                                                                                                                      >  1:284835/1‑107 (MQ=255)                                                                                                                                                                       ttattaGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCAAATAATCTCTTCATCATTTAAGTCTGATAGTAAAt                                                                                                                                                                       <  1:17595/154‑1 (MQ=255)                                                                                                                                                                       ttattaGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCAAATAATCTCTTCATCATTTAAGTCTGATAGTAAATTGAGTAAATGGATAGATTGAGATATTTGTACTTGTTCTTTATTTAATCCATTCGCCACTATAGAGGCAATATGCTCAAGACGCTTTTCACTagcag                                                                       >  1:847768/1‑250 (MQ=255)                                                                                                                                                                             gCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCAAATAATCTCTTCATCATTTAAGTCTGATAGTAAATTGAGTAAATGGATAGATTGAGATATTTGTACTTGTTCTTTATTTAATCCATTCGCCACTATAGAgg                                                                                                     <  1:6535/214‑1 (MQ=255)                                                                                                                                                                               ctctTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCAAATAATCTCTTCATCATTTa                                                                                                                                                                                      >  1:502776/1‑131 (MQ=255)                                                                                                                                                                                    cttctGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCAAATAATCTCTTCATCATTTAAGTCTGATAGTAAATTGAGTAAATGGATAGATTGAGATATTTGTACTTGTTCTTTATTTAATCCATTCGCCACTATAGAgg                                                                                                     <  1:25261/207‑1 (MQ=255)                                                                                                                                                                                             cTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCGGTGTTGGGTGTAAATAAAATCTTAACCAAATAATCTCTTCATCATTTAAGTCTGATAGTAAATTGAGTAAATGGATAGATTGAGATATTTGTACTTGTTCTTTATTTAATCCATTCGCCACTATAGAGGCAATATGCTCAAGACGCTTTTCACTAGCAGCTCGAAAAGCGTAGAATAAAcc                                                 <  1:389556/250‑1 (MQ=255)                                                                                                                                                                                               ccAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCAAATAATCTCTTCATCATTTAAGTCTGATAGTAAATTGAGTAAATGGATAGATTGAGat                                                                                                                                                <  1:11779/153‑1 (MQ=255)                                                                                                                                                                                                  atatAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCAAATAATCTCTTCATCATTTAAGTCTGATAGTAAAt                                                                                                                                                                       >  1:741767/1‑127 (MQ=255)                                                                                                                                                                                                               ctagAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCAAATAATCTCTTCATCATTTAAGTCTGATAGTAAATTGAGTAAATGGATAGATTGAGATATTTGTACTTGTTCTTTATTTAATCCATTCGCCACTATAGAGGCAATATGCTCAAGACGCTTTTCACTAGCAGCTCGAAAAGCGTAGAATAAACCATCTTCAAACAATGCtat                               <  1:432802/250‑1 (MQ=255)                                                                                                                                                                                                                  gagTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCAAATAATCTCTTCATCATTTAAGTCTGATAGTAAATTGAGTAAATGGATAGATTGAGATATTTGTACTTGTTCTTTATTTAATCCATTCGCCACTATag                                                                                                        >  1:435980/1‑174 (MQ=255)                                                                                                                                                                                                                  gagTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCAAATAATCTCTTCATCATTTAAGTCTGATAGTAAATTGAGTAAATGGATAGATTGAGATATTTGTACTTGTTCTTTATTTAATCCATTCGCCACTATAGAGGCAATATGc                                                                                             <  1:796396/185‑1 (MQ=255)                                                                                                                                                                                                                   agTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCAAATAATCTCTTCATCATTTAAGTCTGATAGTAAATTGAGTAAATGGATAGATTGAGATATTTGTACTTGTTCTTTATTTAATCCATTCGCCACTATAGAGGCAATATGCTCAAGACGCTTTTCACTAGCAGCTCGAAAAGCGTAGAATAAACCATCTTCAAACAATGCTatatat                           <  1:48544/250‑1 (MQ=255)                                                                                                                                                                                                                    gTTAAGGTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCAAATAATCTCTTCATCATTTAAGTCTGATAGTAAATTGAGTAAATGGATAGATTGAGATATTTGTACTTGTTCTTTATTTAATCCATTCGCCACTATAGAGGCAATATGCTCAAGACGCTTTTCACTAGCAGCTCGAAAAGCGTAGAATAAACCATCTTCAAACAATGc                                  <  1:361259/242‑1 (MQ=255)                                                                                                                                                                                                                         ggTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCAAATAATCTCTTCATCATTTAAGTCTGATAGTAAATTGAGTAAATGGATAGATTGAGata                                                                                                                                               >  1:483822/1‑128 (MQ=255)                                                                                                                                                                                                                         ggTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCAAATAATCTCTTCATCATTTAAGTCTGATAGTAAATTGAGTAAATGGATAGATTGAGATATTTGTACTTg                                                                                                                                     <  1:737926/138‑1 (MQ=255)                                                                                                                                                                                                                          gTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCAAATAATCTCTTCATCATTTAAGTCTGATAGTAAATTGAGTAAATGGATAGATTGAGATATTTGTACTTGTTCTTTATTTAATCCATTCGCCACTATAGAGGCAATATGCTCAAGACGCTTTTCACTag                                                                          >  1:695655/1‑196 (MQ=255)                                                                                                                                                                                                                          gTACTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCAAATAATCTCTTCATCATTTAAGTCTGATAGTAAATTGAGTAAATGGATAGATTGAGATATTTGTACTTGTTCTTTATTTAATCCATTCGCCACTATAGAGGCAATATGCTCAAGACGCTTTTCACTAGCAGCTCGAAAAGCGTAGAATAAACCATCTTCAAACAATGCTatatat                           >  1:268142/1‑243 (MQ=255)                                                                                                                                                                                                                            aCTCTTATGTTTATCTCTAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCAAATAATCTCTTCATCATTTAAGTCTGATAGTAAATTGAGTAAATGGATAGATTGAGATATTTGTACTTGTTCTTTATTTAATCCATTCGCCACTATAGAGGCAATATGCTCAAGACGCTTTTCACTAGCAGCTCGAAAAGCGTAGAATAAACCATCTTCAAACAATGCtat                               <  1:115358/237‑1 (MQ=255)                                                                                                                                                                                                                                           tctAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCAAATAATCTCTTCATCATTTAAGTCTGATAGTAAATTGAGTAAATGGATAGATTGAGATATTTGTACTTGTTCTTTATTTAATCCATTCGCCACTATAGAGGCAATATGCTCAAGACGCTTTTCACTAGCAGCTCGAAAAGCGTAGAATAAACCATCTTCAAACAATGCTATATATTTAGATAATTTTTCAGCTTCTGAAc  <  1:485092/251‑1 (MQ=255)                                                                                                                                                                                                                                            ctAAATTCCTTATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCAAATAATCTCTTCATCATTTAAGTCTGATAGTAAATTGAGTAAATGGATAGATTGAGATATTTGTACTTGTTCTTTATTTAATCCATTCGCCACTATAGAGGCAATATGCTCAAGACGCTTTTCACTAGCAGCTCGAAAAGCGTAGAATAAACCATCTTCAAACAATGCTATATATTTAGATAATTTTTCAGCTTCTGAAc  >  1:717986/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                CTCTTTAAGCAACATTTTTCCTAAATGAGTTATATATCTAGAACCTTTAGGTTTTCCTGATGATTTATCATAAATAGGCATATTCGTATTTCTATCTATATTAAATTTAGTTTTTATTAAACCTAATCTTTCTAAATGCTCTTTATAGCTCTCTTGAATAGCAGATTTATTAGCCTCTTCTTCTGAAGCTCCAATATAATTTCTAGCTAGAGTTAAGGTACTCTTATGTTTATCTCTAAATTCCTCATCACCACCCAGTGTTGGGTGTAAATAAAATCTTAACCAAATAATCTCTTCATCATTTAAGTCTGATAGTAAATTGAGTAAATGGATAGATTGAGATATTTGTACTTGTTCTTTATTTAATCCATTCGCCACTATAGAGGCAATATGCTCAAGACGCTTTTCACTAGCAGCTCGAAAAGCGTAGAATAAACCATCTTCAAACAATGCTATATATTTAGATAATTTTTCAGCTTCTGAAC  >  4/12812‑13296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑