BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	4	10,616	C→T	intergenic (+203/+9)	unknown → / ← unknown	Porphobilinogen synthase (EC 4.2.1.24)/Glycyl‑tRNA synthetase beta chain (EC 6.1.1.14)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	4	10,616	0	C	T	100.0%	272.5 / NA	100	intergenic (+203/+9)	unknown/unknown	Porphobilinogen synthase (EC 4.2.1.24)/Glycyl‑tRNA synthetase beta chain (EC 6.1.1.14)
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (41/59); total (41/59)

Only 100 of 101 total aligned reads displayed.

AAACAAGTGGCGGAATGGTTGTACTTGGAAGGGAAAAATAAATAGTTCCGATAACATAAAAACGAAAAGCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACACTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTAAGTTCGAGAGTTTATCCAACACGGCGGTGTATTCGCCTTTCGCGATAAGTGGTTGCACTTCCGTTTGTAACACAAGCACGGCTTCGGCGAG  >  4/10369‑10855

aaaCAAGTGGCGGAATGGTTGTACTTGGAAGGGAAAAATAAATAGTTCCGATAACATAAAAACGAAAAGCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACAtttt                                                                                                                                                                                                                                               >  1:617217/1‑251 (MQ=255)

   cAAGTGGCGGAATGGTTGTACTTGGAAGGGAAAAATAAATAGTTCCGATAACATAAAAACGAAAAGCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGGGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTgg                                                                                                                                                                                                                                            >  1:883766/1‑251 (MQ=255)

              aTGGTTGTACTTGGAAGGGAAAAATAAATAGTTCCGATAACATAAAAACGAAAAGCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGcaa                                                                                                                                                                                                                                 <  1:274136/251‑1 (MQ=255)

               tGGTTGTACTTGGAAGGGAAAAATAAATAGTTCCGATAACATAAAAACGAAAAGCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGcaa                                                                                                                                                                                                                                 >  1:446464/1‑250 (MQ=255)

                 gTTGTACTTGGAAGGGAAAAATAAATAGTTCCGATAACATAAAAACGAAAAGCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGAGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGcaacaa                                                                                                                                                                                                                              >  1:405768/1‑251 (MQ=255)

                      aCTTGGAAGGGAAAAATAAATAGTTCCGATAACATAAAAACGAAAAGCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGaaa                                                                                                                                                                                                                         <  1:62539/251‑1 (MQ=255)

                       cTTGGAAGGGAAAAATAAATAGTTCCGATAACATAAAAACGAAAAGCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAat                                                                                                                                                                                                                        >  1:240727/1‑251 (MQ=255)

                           gAAGGGAAAAATAAATAGTTCCGATAACATAAAAACGAAAAGCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATc                                                                                                                                                                                                                     >  1:352753/1‑250 (MQ=255)

                           gAAGGGAAAAATAAATAGTTCCGATAACATAAAAACGAAAAGCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATc                                                                                                                                                                                                                     <  1:340701/250‑1 (MQ=255)

                                  aaaaTAAATAGTTCCGATAACATAAAAACGAAAAGCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAatat                                                                                                                                                                                                                      >  1:648653/1‑242 (MQ=255)

                                  aaaaTAAATAGTTCCGATAACATAAAAACGAAAAGCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCAc                                                                                                                                                                                                               >  1:54875/1‑249 (MQ=255)

                                  aaaaTAAATAGTTCCGATAACATAAAAACGAAAAGCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCAc                                                                                                                                                                                                               >  1:144398/1‑249 (MQ=255)

                                    aataaataGTTCCGATAACATAAAAACGAAAAGCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCAc                                                                                                                                                                                                               >  1:257283/1‑247 (MQ=255)

                                       aaataGTTCCGATAACATAAAAACGAAAAGCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAg                                                                                                                                                                                                       <  1:563387/251‑1 (MQ=255)

                                         ataGTTCCGATAACATAAAAACGAAAAGCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGaa                                                                                                                                                                                                     <  1:125631/251‑1 (MQ=255)

                                            gTTCCGATAACATAAAAACGAAAAGCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGGGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCaacaac                                                                                                                                                                                                                             >  1:624654/1‑225 (MQ=255)

                                               ccGATAACATAAAAACGAAAAGCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAAcc                                                                                                                                                                                               <  1:242511/251‑1 (MQ=255)

                                               ccGATAACATAAAAACGAAAAGCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAAcc                                                                                                                                                                                               <  1:747664/251‑1 (MQ=255)

                                                cGATAACATAAAAACGAAAAGCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAAc                                                                                                                                                                                                >  1:620623/1‑249 (MQ=255)

                                                cGATAACATAAAAACGAAAAGCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCt                                                                                                                                                                                              <  1:875987/251‑1 (MQ=255)

                                                     aCATAAAAACGAAAAGCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAg                                                                                                                                                                                         <  1:569080/251‑1 (MQ=255)

                                                      cATAAAAACGAAAAGCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTCAAATCACGAGCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGc                                                                                                                                                                                        <  1:522359/251‑1 (MQ=255)

                                                        tAAAAACGAAAAGCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGt                                                                                                                                                                                      <  1:319458/251‑1 (MQ=255)

                                                            aaCGAAAAGCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTc                                                                                                                                                                                  <  1:475274/251‑1 (MQ=255)

                                                                aaaaGCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCaaaa                                                                                                                                                                              <  1:514931/251‑1 (MQ=255)

                                                                    gCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTg                                                                                                                                                                                                                                    <  1:400192/194‑1 (MQ=255)

                                                                         atatGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGCCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAg                                                                                                                                                                                         <  1:435600/231‑1 (MQ=255)

                                                                           atGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCgg                                                                                                                                                                   >  1:809748/1‑251 (MQ=255)

                                                                              ttATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGttt                                                                                                                                                                >  1:79372/1‑251 (MQ=255)

                                                                              ttATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGttt                                                                                                                                                                >  1:795932/1‑251 (MQ=255)

                                                                                aTTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTg                                                                                                                                                              >  1:851431/1‑251 (MQ=255)

                                                                                    ggCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCACAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGACTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAg                                                                                                                                                                                         >  1:423327/1‑220 (MQ=255)

                                                                                     gCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAg                                                                                                                                                                                         >  1:272731/1‑219 (MQ=255)

                                                                                     gCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGa                                                                                                                                                         >  1:767784/1‑251 (MQ=255)

                                                                                           tatttatGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAAT‑AACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTgg                                                                                                                                                  <  1:238548/251‑1 (MQ=255)

                                                                                             tttatGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCG‑ATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGAt                                                                                                                                                <  1:835046/251‑1 (MQ=255)

                                                                                              ttatGTGAAAAGTGTGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGAt                                                                                                                                                <  1:265853/251‑1 (MQ=255)

                                                                                              ttatGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAGCCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGAt                                                                                                                                                <  1:759596/251‑1 (MQ=255)

                                                                                              ttatGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGAt                                                                                                                                                <  1:757885/251‑1 (MQ=255)

                                                                                              ttatGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGAt                                                                                                                                                <  1:570954/251‑1 (MQ=255)

                                                                                              ttatGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGAt                                                                                                                                                <  1:898291/251‑1 (MQ=255)

                                                                                              ttatGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGAt                                                                                                                                                <  1:890409/251‑1 (MQ=255)

                                                                                              ttatGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGAt                                                                                                                                                <  1:340167/251‑1 (MQ=255)

                                                                                              ttatGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGAt                                                                                                                                                <  1:731045/251‑1 (MQ=255)

                                                                                                atGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAAt                                                                                                                                                                             >  1:358874/1‑220 (MQ=255)

                                                                                                 tgtgAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGcaac                                                                                                                                                                                                                                >  1:276714/1‑169 (MQ=255)

                                                                                                 tgtgAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCgg                                                                                                                                                          >  1:705489/1‑238 (MQ=255)

                                                                                                      aaaGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCg                                                                                                                                        <  1:347109/251‑1 (MQ=255)

                                                                                                             ggTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCa                                                                                                                                 >  1:284752/1‑251 (MQ=255)

                                                                                                                  gAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATaa                                                                                                                                                                                                  <  1:623442/181‑1 (MQ=255)

                                                                                                                        aCGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCg                                                                                                                      <  1:179791/251‑1 (MQ=255)

                                                                                                                           gCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCgaag                                                                                                                   >  1:486694/1‑251 (MQ=255)

                                                                                                                           gCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCgaag                                                                                                                   >  1:809155/1‑251 (MQ=255)

                                                                                                                              ttttttAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGaagaag                                                                                                                <  1:856716/251‑1 (MQ=255)

                                                                                                                               tttttAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGaagaag                                                                                                                >  1:780331/1‑250 (MQ=255)

                                                                                                                                ttttAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGtt                                                                                                              >  1:97982/1‑251 (MQ=255)

                                                                                                                                ttttAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGtt                                                                                                              >  1:100059/1‑251 (MQ=255)

                                                                                                                                ttttAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGtt                                                                                                              >  1:267848/1‑251 (MQ=255)

                                                                                                                                       cccTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCAcc                                                                                                       <  1:169787/251‑1 (MQ=255)

                                                                                                                                           cAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAg                                                                                                   <  1:443814/251‑1 (MQ=255)

                                                                                                                                             aCCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAGCG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGAtct                                                                                                                                              <  1:686021/206‑1 (MQ=255)

                                                                                                                                             aCCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCAcc                                                                                                                                                                                                              >  1:276861/1‑143 (MQ=255)

                                                                                                                                             aCCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGaa                                                                                                                                                                                                     <  1:609524/151‑1 (MQ=255)

                                                                                                                                             aCCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCaaa                                                                                                                                                                               <  1:626108/173‑1 (MQ=255)

                                                                                                                                             aCCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGt                                                                                                                           >  1:371268/1‑225 (MQ=255)

                                                                                                                                             aCCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTa                                                                                                 >  1:559243/1‑251 (MQ=255)

                                                                                                                                                tGAAATCAGGATCGGTGACCTTGTCCACCCATAAGTGATTTTACCAAAG‑ACGAAGGCAAGATTCTTGC‑TCGAACAAGTTCAGAAACCGTCTTGATTTGTCCCATTTTTGGCTTATTTGAAACAAGGAATTATCGGCC‑CCTGTTAAG‑ATTAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTa                                                                                             <  1:320121/251‑1 (MQ=255)

                                                                                                                                                tGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGt                                                                                              <  1:635443/251‑1 (MQ=255)

                                                                                                                                                 gAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGa                                                                                                                                                                                                      >  1:648787/1‑146 (MQ=255)

                                                                                                                                                 gAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGAt                                                                                                                                                <  1:12353/200‑1 (MQ=255)

                                                                                                                                                  aaaTCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTa                                                                                             <  1:861911/250‑1 (MQ=255)

                                                                                                                                                  aaaTCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTa                                                                                             <  1:624598/250‑1 (MQ=255)

                                                                                                                                                   aaTCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTAAg                                                                                           <  1:857705/251‑1 (MQ=255)

                                                                                                                                                     tCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTAAGtt                                                                                         <  1:92323/251‑1 (MQ=255)

                                                                                                                                                        cGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTAAGTTCg                                                                                       >  1:708825/1‑250 (MQ=255)

                                                                                                                                                              gTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCgaagaa                                                                                                                 <  1:342464/218‑1 (MQ=255)

                                                                                                                                                                  aCTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTAAGTTCGCGAGTTTATcc                                                                            <  1:44130/251‑1 (MQ=255)

                                                                                                                                                                  aCTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTAAGTTCGCGAGTTTATcc                                                                            <  1:617629/251‑1 (MQ=255)

                                                                                                                                                                       tCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTAAGTTCGCGAGTTTATCCAacac                                                                       <  1:113506/251‑1 (MQ=255)

                                                                                                                                                                            ccATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTAAGTTCGCGAGTTTATCCAACAcggcgg                                                                  >  1:652757/1‑251 (MQ=255)

                                                                                                                                                                             cATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCAcc                                                                                                                                  >  1:25884/1‑186 (MQ=255)

                                                                                                                                                                               tAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTAAGTTCGCGAGTTTATCCAACACGGCGgtgt                                                               <  1:731289/251‑1 (MQ=255)

                                                                                                                                                                                     aTTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTAAGTTCGCGAGTTTATCCAACACGGCGGTGTATTCGc                                                         <  1:50242/251‑1 (MQ=255)

                                                                                                                                                                                          aaCTAAGAACG‑ACGCAAGATTCATGCTTCG‑TCAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTAAGTTCGCGAGTTTATcc                                                                            >  1:838509/1‑227 (MQ=255)

                                                                                                                                                                                           aCTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTAAGTTCGCGAGTTTATCCAacac                                                                       <  1:571488/231‑1 (MQ=255)

                                                                                                                                                                                           aCTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTAAGTTCGCGAGTTTATCCAACACGGCGGTGTATTCGCCTTTcg                                                   >  1:527162/1‑251 (MQ=255)

                                                                                                                                                                                             tAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGT‑TTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTAAGTTCGCGAGTTTATCCAACACGGCGGTGTATTCGCCTTTCGCGa                                                <  1:557363/251‑1 (MQ=255)

                                                                                                                                                                                                  acg‑acgCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTAAGTTCGCGAGTTTATCCAACACGGCGGTGTATTCGCCTTTCGCGATAAg                                            <  1:377918/251‑1 (MQ=255)

                                                                                                                                                                                                      acgCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGAt                                                                                                                                                <  1:532831/148‑1 (MQ=255)

                                                                                                                                                                                                      acgCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTAAGTTCGCGAGTTTATCCAACACGGCGGTGTATTCGCCTTTCGCGATAAGTgg                                         >  1:779508/1‑251 (MQ=255)

                                                                                                                                                                                                      acgCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTAAGTTCGCGAGTTTATCCAACACGGCGGTGTATTCGCCTTTCGCGATAAGTg                                          <  1:136998/250‑1 (MQ=255)

                                                                                                                                                                                                                 aTGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTAAGTTCGCGAGTTTATCCAACACGGCGGTGTATTCGCCTTTCGCGATAAGTGGTTGCACTTCCg                              <  1:603576/251‑1 (MQ=255)

                                                                                                                                                                                                                              gCTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTAAGTTCGCGAGTTTATCCAACACGGCGGTGTATTCGcc                                                        <  1:229539/213‑1 (MQ=255)

                                                                                                                                                                                                                               cTCAGCAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTAAGTTCGCGAGTTTATCCAACACGGCGGTGTATTCGCCTTTCGCGATAAGTGGTTGCACTTCCGTTTGTAac                      <  1:539333/246‑1 (MQ=255)

                                                                                                                                                                                                                                    cAACCGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTAAGTTCGCGAGTTTATCCAACACGGCGGTGTATTCGCCTTTCGCGATAAGTGGTTGCACTTCCGTTTGTAACACAAGCAc              <  1:151548/249‑1 (MQ=255)

                                                                                                                                                                                                                                       ccGTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTAAGTTCGCGAGTTTATCCAACACGGCGGTGTATTCGCCTTTCGCGATAAGTGGTTGCACTTCCGTTTGTAACACAAGCACGGCtt         >  1:799245/1‑251 (MQ=255)

                                                                                                                                                                                                                                         gTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTAAGTTCGCGAGTTTATCCAac                                                                         <  1:549929/185‑1 (MQ=255)

                                                                                                                                                                                                                                         gTCTTGCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTAAGTTCGCGAGTTTATCCAACACGGCGGTGTAtt                                                            >  1:226183/1‑198 (MQ=255)

                                                                                                                                                                                                                                              gCATTGTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTAAGTTCGCGAGTTTATCCAACACGGCGGTGTATTCGCCTTTCGCGATAAGTGGTTGCACTTCCGTTTGTAACACAAGCACGGCTTCGGCGAg  >  1:242192/1‑251 (MQ=255)

                                                                                                                                                                                                                                                   gTCACATTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTAAGTTCGCGAGTTTATCCAACACGGCGGTGTATTCGCCTTTCGCGATAAGTGGTTGCACTTCCGTTTGTAac                      <  1:402086/226‑1 (MQ=255)

AAACAAGTGGCGGAATGGTTGTACTTGGAAGGGAAAAATAAATAGTTCCGATAACATAAAAACGAAAAGCCGAATATGTTATTCGGCTTTTTATTTATGTGAAAAGTGCGGTCAGAATTGACGGCGTTTTTTAGTCCCTCAACCTGAAATCACGATCGGTGAACTTGTCGAACCATAAGTGATTTTAACTAAGAACG‑ACGCAAGATTCATGCTTCG‑ACAAGCTCAGCAACCGTCTTGCATTGTCACACTTTTGGCTTTATTGCAACAACGAAATATCCGCCACCTG‑TAAGAATAAACCTTGTAGCGTATTCAAAATCGCCAAGCGGTTTTGGCGGAGTGTTGGATCTTCTGCGTTCACCATCACGTTGTCGAAGAAGTTATCCACCGGAGTACGTAAGTTCGAGAGTTTATCCAACACGGCGGTGTATTCGCCTTTCGCGATAAGTGGTTGCACTTCCGTTTGTAACACAAGCACGGCTTCGGCGAG  >  4/10369‑10855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`