Predicted mutation
evidence seq id position mutation annotation gene description
RA 4 7,124 T→C R240R (CGT→CGC)  unknown → 33 kDa chaperonin HslO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*47,1240TC100.0% 172.5 / NA 63R240R (CGT→CGC) unknown33 kDa chaperonin HslO
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (52/11);  total (52/11)

CATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTCGAACTCTTCCTGCCGCAAACAGTAAGCTTCCACTGTGGTTGCTCGCAAGAACGTTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAATTTTTATGCAATATCGTTGCTGTAGACAAACAAAAACTGCCTTACTGG  >  4/6881‑7368
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    
catTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCgggg                                                                                                                                                                                                                                               >  1:31606/1‑249 (MQ=255)
      cACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCcgcg                                                                                                                                                                                                                                          >  1:449607/1‑250 (MQ=255)
                    aCGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTc                                                                                                                                                                                                                            >  1:490088/1‑250 (MQ=255)
                              ggTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATAACGTTTATATCACGAAGAAAGCGTGGAAATCTTCCCGCCACAAGCGGTAAGTTTTCAATGCGGTTGCTCGCAAAAACGCTCCGGCGCCGCGCTGTTATTGAATTCCGAAGGAGaaa                                                                                                                                                                                                                 >  1:663190/1‑251 (MQ=255)
                               gTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAAt                                                                                                                                                                                                                >  1:518737/1‑251 (MQ=255)
                                   gcgcGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGGAAGTTTTCACTGCGGTTTCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGaa                                                                                                                                                                                                            >  1:381989/1‑251 (MQ=255)
                                        ggCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAAt                                                                                                                                                                                                       >  1:682309/1‑251 (MQ=255)
                                        ggCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAAt                                                                                                                                                                                                       >  1:777509/1‑251 (MQ=255)
                                                                    gttccggttcACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGat                                                                                                                                                                           >  1:8157/1‑251 (MQ=255)
                                                                              aCCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCg                                                                                                                                                                 >  1:828565/1‑251 (MQ=255)
                                                                               ccGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGc                                                                                                                                                                                    >  1:245482/1‑231 (MQ=255)
                                                                               ccGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGc                                                                                                                                                                       >  1:838897/1‑244 (MQ=255)
                                                                               ccGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGa                                                                                                                                                                >  1:834870/1‑251 (MQ=255)
                                                                                                        cGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGACGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGtt                                                                                                                                        >  1:434637/1‑250 (MQ=255)
                                                                                                        cGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGtt                                                                                                                                        >  1:210798/1‑250 (MQ=255)
                                                                                                            aaCGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTc                                                                                                                                       >  1:37248/1‑247 (MQ=255)
                                                                                                            aaCGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATa                                                                                                                                   >  1:494499/1‑251 (MQ=255)
                                                                                                            aaCGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATa                                                                                                                                   >  1:370980/1‑251 (MQ=255)
                                                                                                              cGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGc                                                                                                                                                                                                 >  1:19343/1‑187 (MQ=255)
                                                                                                                    ccATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGAt                                                                                                                                                                                                                               >  1:612844/1‑151 (MQ=255)
                                                                                                                    ccATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGc                                                                                                                                                                                                 >  1:675847/1‑181 (MQ=255)
                                                                                                                    ccATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCg                                                                                                                           >  1:193732/1‑251 (MQ=255)
                                                                                                                    ccATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCg                                                                                                                           >  1:3216/1‑251 (MQ=255)
                                                                                                                     cATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTg                                                                                                                                          >  1:777609/1‑235 (MQ=255)
                                                                                                                                            gTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCgg                                                                                                     >  1:11487/1‑249 (MQ=255)
                                                                                                                                              ttAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTg                                                                                                                                          >  1:104653/1‑210 (MQ=255)
                                                                                                                                                          gaaTTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACg                                                                                     >  1:649633/1‑251 (MQ=255)
                                                                                                                                                             ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAAtgc                                                                                                                                                            >  1:140040/1‑177 (MQ=255)
                                                                                                                                                             ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCattatt                                                                                                                                            >  1:587459/1‑193 (MQ=255)
                                                                                                                                                             ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAgg                                                                                                                             >  1:218447/1‑208 (MQ=255)
                                                                                                                                                             ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCg                                                                                  >  1:726706/1‑251 (MQ=255)
                                                                                                                                                             ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCg                                                                                  >  1:659612/1‑251 (MQ=255)
                                                                                                                                                             ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCg                                                                                  >  1:14724/1‑251 (MQ=255)
                                                                                                                                                             ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATACGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCg                                                                                  >  1:433478/1‑251 (MQ=255)
                                                                                                                                                               gTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGaa                                                                                >  1:238204/1‑251 (MQ=255)
                                                                                                                                                                  ataCCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAAttt                                                                             >  1:467549/1‑250 (MQ=255)
                                                                                                                                                                      cGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGc                                                                                                                                                                       >  1:546976/1‑157 (MQ=255)
                                                                                                                                                                       gTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCa                                                                                                                                                                                        >  1:641554/1‑139 (MQ=255)
                                                                                                                                                                       gTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAAt                                                                         >  1:58008/1‑250 (MQ=255)
                                                                                                                                                                                            tgaacccctccccgccacaagcggTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTc                                                    <  1:578483/227‑1 (MQ=255)
                                                                                                                                                                                                   cttcccgccacaagcggTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATACTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGc                                                               >  1:887247/17‑232 (MQ=255)
                                                                                                                                                                                                        cgccacaagcggTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCattatt                                                                                                                                            <  1:159921/139‑1 (MQ=255)
                                                                                                                                                                                                        cgccacaagcggTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAAtttt                                              >  1:222518/12‑244 (MQ=255)
                                                                                                                                                                                                        cgccacaagcggTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAATTTTTATGCaa                                       >  1:113202/12‑251 (MQ=255)
                                                                                                                                                                                                            acaagcggTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGA‑TTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAATTTTTATGCAATATCg                                  <  1:521073/244‑1 (MQ=255)
                                                                                                                                                                                                               agcggTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAATTTTTATGCAATATCGtt                                >  1:570074/5‑251 (MQ=255)
                                                                                                                                                                                                                 cggTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGc                                                                                                                                                                       >  1:189800/3‑114 (MQ=255)
                                                                                                                                                                                                                 cggTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAATTTTTATGCAATATCGTTg                               <  1:394738/248‑1 (MQ=255)
                                                                                                                                                                                                                 cggTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAATTTTTATGCAATATCGTTg                               >  1:70957/3‑250 (MQ=255)
                                                                                                                                                                                                                  ggTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCaa                                                                                                                                                                     <  1:730855/114‑1 (MQ=255)
                                                                                                                                                                                                                   gTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAATTTTTATGCAATATCGTTg                               >  1:649088/1‑248 (MQ=255)
                                                                                                                                                                                                                     aagttttcACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAATTTTTATGCAATATCGTTGCTGTa                          >  1:192229/8‑251 (MQ=255)
                                                                                                                                                                                                                       gttttcACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAATTTTTATGCAATATCGTTGCTGTAGa                        >  1:132612/6‑251 (MQ=255)
                                                                                                                                                                                                                       gttttcACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAATTTTTATGCAATATCGTTGCTGTAGa                        >  1:763037/6‑251 (MQ=255)
                                                                                                                                                                                                                        ttttcACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAgg                                                                                           >  1:317368/5‑183 (MQ=255)
                                                                                                                                                                                                                        ttttcACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAATTTTTATGCAACATCGTTGCTGTAg                         <  1:478762/245‑1 (MQ=255)
                                                                                                                                                                                                                            cACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAATTTTTATGCAATATCGTTGCTGTAGacaaac                   <  1:630020/251‑1 (MQ=255)
                                                                                                                                                                                                                                      gCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTg                                                                                                                                          >  1:174515/1‑122 (MQ=255)
                                                                                                                                                                                                                                       cTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAATTTTTATGCAATATCGTTGCTGTAGACAAACAAAAACTGcc         <  1:751916/250‑1 (MQ=255)
                                                                                                                                                                                                                                         cGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAATTTTTATGCAATATCGTTGCTGTAGACAAACAAAAACTGCCTTa      >  1:106462/1‑251 (MQ=255)
                                                                                                                                                                                                                                           cAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAAtttt                                              <  1:285330/209‑1 (MQ=255)
                                                                                                                                                                                                                                           cAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAATTTTTATGCAATATCGTTGCTGTAGACAAACAAAAACTGCCTTAc     <  1:552597/250‑1 (MQ=255)
                                                                                                                                                                                                                                               aaCGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAATTTTTATGCAATATCGTTGCTGTAGACAAACAAAAACTGCCTTACTgg  <  1:844108/249‑1 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    
CATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTCGAACTCTTCCTGCCGCAAACAGTAAGCTTCCACTGTGGTTGCTCGCAAGAACGTTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAATTTTTATGCAATATCGTTGCTGTAGACAAACAAAAACTGCCTTACTGG  >  4/6881‑7368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑