Predicted mutation
evidence seq id position mutation annotation gene description
RA 4 7,097 C→T S231S (AGC→AGT)  unknown → 33 kDa chaperonin HslO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*47,0970CT98.3% 144.6 / NA 59S231S (AGC→AGT) unknown33 kDa chaperonin HslO
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (53/5);  total (54/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTCCGAACAGCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTCGAACTCTTCCTGCCGCAAACAGTAAGCTTCCACTGTGGTTGCTCGCAAGAACGTTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAATTTTTATGCAATATCGTTG  >  4/6853‑7339
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  
gcTCCGAACAGCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGCTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCCAGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGCtttca                                                                                                                                                                                                                                               >  1:646568/1‑247 (MQ=255)
     gAACAGCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACtgcgg                                                                                                                                                                                                                                         >  1:149220/1‑248 (MQ=255)
        cAGCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTg                                                                                                                                                                                                                                      >  1:189749/1‑251 (MQ=255)
          gCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTg                                                                                                                                                                                                                                      >  1:724829/1‑249 (MQ=255)
          gCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTg                                                                                                                                                                                                                                      >  1:626191/1‑249 (MQ=255)
          gCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGAAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTg                                                                                                                                                                                                                                      >  1:427845/1‑249 (MQ=255)
                      gCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCCCAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACgc                                                                                                                                                                                                                         >  1:289226/1‑249 (MQ=255)
                       cTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACt                                                                                                                                                                                                                                             >  1:108758/1‑229 (MQ=255)
                            catTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCgggg                                                                                                                                                                                                                  >  1:31606/1‑249 (MQ=255)
                                  cACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCcgcg                                                                                                                                                                                                             >  1:449607/1‑250 (MQ=255)
                                                aCGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTc                                                                                                                                                                                               >  1:490088/1‑250 (MQ=255)
                                                          ggTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATAACGTTTATATCACGAAGAAAGCGTGGAAATCTTCCCGCCACAAGCGGTAAGTTTTCAATGCGGTTGCTCGCAAAAACGCTCCGGCGCCGCGCTGTTATTGAATTCCGAAGGAGaaa                                                                                                                                                                                    >  1:663190/1‑251 (MQ=255)
                                                           gTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAAt                                                                                                                                                                                   >  1:518737/1‑251 (MQ=255)
                                                               gcgcGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGGAAGTTTTCACTGCGGTTTCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGaa                                                                                                                                                                               >  1:381989/1‑251 (MQ=255)
                                                                    ggCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAAt                                                                                                                                                                          >  1:682309/1‑251 (MQ=255)
                                                                    ggCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAAt                                                                                                                                                                          >  1:777509/1‑251 (MQ=255)
                                                                                                gttccggttcACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGat                                                                                                                                              >  1:8157/1‑251 (MQ=255)
                                                                                                          aCCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCg                                                                                                                                    >  1:828565/1‑251 (MQ=255)
                                                                                                           ccGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGc                                                                                                                                                       >  1:245482/1‑231 (MQ=255)
                                                                                                           ccGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGc                                                                                                                                          >  1:838897/1‑244 (MQ=255)
                                                                                                           ccGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGa                                                                                                                                   >  1:834870/1‑251 (MQ=255)
                                                                                                                                    cGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGACGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGtt                                                                                                           >  1:434637/1‑250 (MQ=255)
                                                                                                                                    cGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGtt                                                                                                           >  1:210798/1‑250 (MQ=255)
                                                                                                                                        aaCGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTc                                                                                                          >  1:37248/1‑247 (MQ=255)
                                                                                                                                        aaCGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATa                                                                                                      >  1:370980/1‑251 (MQ=255)
                                                                                                                                        aaCGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATa                                                                                                      >  1:494499/1‑251 (MQ=255)
                                                                                                                                          cGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGc                                                                                                                                                                    >  1:19343/1‑187 (MQ=255)
                                                                                                                                                ccATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGAt                                                                                                                                                                                                  >  1:612844/1‑151 (MQ=255)
                                                                                                                                                ccATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGc                                                                                                                                                                    >  1:675847/1‑181 (MQ=255)
                                                                                                                                                ccATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCg                                                                                              >  1:193732/1‑251 (MQ=255)
                                                                                                                                                ccATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCg                                                                                              >  1:3216/1‑251 (MQ=255)
                                                                                                                                                 cATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTg                                                                                                             >  1:777609/1‑235 (MQ=255)
                                                                                                                                                                        gTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCgg                                                                        >  1:11487/1‑249 (MQ=255)
                                                                                                                                                                          ttAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTg                                                                                                             >  1:104653/1‑210 (MQ=255)
                                                                                                                                                                                      gaaTTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACg                                                        >  1:649633/1‑251 (MQ=255)
                                                                                                                                                                                         ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAAtgc                                                                                                                               >  1:140040/1‑177 (MQ=255)
                                                                                                                                                                                         ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCattatt                                                                                                               >  1:587459/1‑193 (MQ=255)
                                                                                                                                                                                         ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAgg                                                                                                >  1:218447/1‑208 (MQ=255)
                                                                                                                                                                                         ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCg                                                     >  1:14724/1‑251 (MQ=255)
                                                                                                                                                                                         ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCg                                                     >  1:659612/1‑251 (MQ=255)
                                                                                                                                                                                         ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCg                                                     >  1:726706/1‑251 (MQ=255)
                                                                                                                                                                                         ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATACGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCg                                                     >  1:433478/1‑251 (MQ=255)
                                                                                                                                                                                           gTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGaa                                                   >  1:238204/1‑251 (MQ=255)
                                                                                                                                                                                              ataCCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAAttt                                                >  1:467549/1‑250 (MQ=255)
                                                                                                                                                                                                  cGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGc                                                                                                                                          >  1:546976/1‑157 (MQ=255)
                                                                                                                                                                                                   gTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCa                                                                                                                                                           >  1:641554/1‑139 (MQ=255)
                                                                                                                                                                                                   gTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAAt                                            >  1:58008/1‑250 (MQ=255)
                                                                                                                                                                                                                        tgaacccctccccgccacaagcggTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTc                       <  1:578483/227‑1 (MQ=255)
                                                                                                                                                                                                                               cttcccgccacaagcggTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATACTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGc                                  >  1:887247/17‑232 (MQ=255)
                                                                                                                                                                                                                                    cgccacaagcggTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCattatt                                                                                                               <  1:159921/139‑1 (MQ=255)
                                                                                                                                                                                                                                    cgccacaagcggTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAAtttt                 >  1:222518/12‑244 (MQ=255)
                                                                                                                                                                                                                                    cgccacaagcggTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAATTTTTATGCaa          >  1:113202/12‑251 (MQ=255)
                                                                                                                                                                                                                                        acaagcggTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGA‑TTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAATTTTTATGCAATATCg     <  1:521073/244‑1 (MQ=255)
                                                                                                                                                                                                                                           agcggTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAATTTTTATGCAATATCGtt   >  1:570074/5‑251 (MQ=255)
                                                                                                                                                                                                                                             cggTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGc                                                                                                                                          >  1:189800/3‑114 (MQ=255)
                                                                                                                                                                                                                                             cggTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAATTTTTATGCAATATCGTTg  >  1:70957/3‑250 (MQ=255)
                                                                                                                                                                                                                                             cggTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAATTTTTATGCAATATCGTTg  <  1:394738/248‑1 (MQ=255)
                                                                                                                                                                                                                                              ggTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCaa                                                                                                                                        <  1:730855/114‑1 (MQ=255)
                                                                                                                                                                                                                                               gTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAATTTTTATGCAATATCGTTg  >  1:649088/1‑248 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  
GCTCCGAACAGCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTCGAACTCTTCCTGCCGCAAACAGTAAGCTTCCACTGTGGTTGCTCGCAAGAACGTTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAATCCGGTTAAGCAAAGTACGGTCAATTTTTATGCAATATCGTTG  >  4/6853‑7339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑