Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	4	7,085	G→A	P227P (CCG→CCA)	unknown →	33 kDa chaperonin HslO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	4	7,085	0	G	A	98.1%	143.8 / NA	52	P227P (CCG→CCA)	unknown	33 kDa chaperonin HslO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (51/0);  minor base C (1/0);  total (52/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTCCGAACAGCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTCGAACTCTTCCTGCCGCAAACAGTAAGCTTCCACTGTGGTTGCTCGCAAGAACGTTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAAT  >  4/6853‑7297                                                                                                                                                                                                                                         |                                                                                                                                                                                                                     gcTCCGAACAGCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGCTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCCAGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGCtttca                                                                                                                                                                                                     >  1:646568/1‑247 (MQ=255)    ccGAACAGCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGGTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACaagcgggaaggtttcactg                                                                                                                                                                                                  >  1:901760/1‑233 (MQ=255)      gAACAGCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACtgcgg                                                                                                                                                                                               >  1:149220/1‑248 (MQ=255)         cAGCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTg                                                                                                                                                                                            >  1:189749/1‑251 (MQ=255)           gCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACaagcggta                                                                                                                                                                                                             >  1:490698/1‑226 (MQ=255)           gCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACaagc                                                                                                                                                                                                                 >  1:45500/1‑226 (MQ=255)           gCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTg                                                                                                                                                                                            >  1:724829/1‑249 (MQ=255)           gCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTg                                                                                                                                                                                            >  1:626191/1‑249 (MQ=255)           gCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGAAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTg                                                                                                                                                                                            >  1:427845/1‑249 (MQ=255)                       gCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCCCAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACgc                                                                                                                                                                               >  1:289226/1‑249 (MQ=255)                        cTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACt                                                                                                                                                                                                   >  1:108758/1‑229 (MQ=255)                             catTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCgggg                                                                                                                                                                        >  1:31606/1‑249 (MQ=255)                                   cACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCcgcg                                                                                                                                                                   >  1:449607/1‑250 (MQ=255)                                                 aCGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTc                                                                                                                                                     >  1:490088/1‑250 (MQ=255)                                                           ggTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATAACGTTTATATCACGAAGAAAGCGTGGAAATCTTCCCGCCACAAGCGGTAAGTTTTCAATGCGGTTGCTCGCAAAAACGCTCCGGCGCCGCGCTGTTATTGAATTCCGAAGGAGaaa                                                                                                                                          >  1:663190/1‑251 (MQ=255)                                                            gTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAAt                                                                                                                                         >  1:518737/1‑251 (MQ=255)                                                                gcgcGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGGAAGTTTTCACTGCGGTTTCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGaa                                                                                                                                     >  1:381989/1‑251 (MQ=255)                                                                     ggCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAAt                                                                                                                                >  1:777509/1‑251 (MQ=255)                                                                     ggCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAAt                                                                                                                                >  1:682309/1‑251 (MQ=255)                                                                                                 gttccggttcACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGat                                                                                                    >  1:8157/1‑251 (MQ=255)                                                                                                           aCCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCg                                                                                          >  1:828565/1‑251 (MQ=255)                                                                                                            ccGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAgttttcac                                                                                                                                                                                                    >  1:524866/1‑137 (MQ=255)                                                                                                            ccGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGc                                                                                                             >  1:245482/1‑231 (MQ=255)                                                                                                            ccGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGc                                                                                                >  1:838897/1‑244 (MQ=255)                                                                                                            ccGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGa                                                                                         >  1:834870/1‑251 (MQ=255)                                                                                                                                     cGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGACGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGtt                                                                 >  1:434637/1‑250 (MQ=255)                                                                                                                                     cGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGtt                                                                 >  1:210798/1‑250 (MQ=255)                                                                                                                                         aaCGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTc                                                                >  1:37248/1‑247 (MQ=255)                                                                                                                                         aaCGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATa                                                            >  1:494499/1‑251 (MQ=255)                                                                                                                                         aaCGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATa                                                            >  1:370980/1‑251 (MQ=255)                                                                                                                                           cGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGc                                                                                                                          >  1:19343/1‑187 (MQ=255)                                                                                                                                                 ccATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAgttttcac                                                                                                                                                                                                    >  1:753301/1‑100 (MQ=255)                                                                                                                                                 ccATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGAt                                                                                                                                                        >  1:612844/1‑151 (MQ=255)                                                                                                                                                 ccATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGc                                                                                                                          >  1:675847/1‑181 (MQ=255)                                                                                                                                                 ccATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCg                                                    >  1:3216/1‑251 (MQ=255)                                                                                                                                                 ccATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCg                                                    >  1:193732/1‑251 (MQ=255)                                                                                                                                                  cATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTg                                                                   >  1:777609/1‑235 (MQ=255)                                                                                                                                                                         gTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCgg                              >  1:11487/1‑249 (MQ=255)                                                                                                                                                                           ttAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTg                                                                   >  1:104653/1‑210 (MQ=255)                                                                                                                                                                                       gaaTTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACg              >  1:649633/1‑251 (MQ=255)                                                                                                                                                                                          ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAAtgc                                                                                     >  1:140040/1‑177 (MQ=255)                                                                                                                                                                                          ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCattatt                                                                     >  1:587459/1‑193 (MQ=255)                                                                                                                                                                                          ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAgg                                                      >  1:218447/1‑208 (MQ=255)                                                                                                                                                                                          ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCg           >  1:726706/1‑251 (MQ=255)                                                                                                                                                                                          ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCg           >  1:14724/1‑251 (MQ=255)                                                                                                                                                                                          ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCg           >  1:659612/1‑251 (MQ=255)                                                                                                                                                                                          ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATACGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCg           >  1:433478/1‑251 (MQ=255)                                                                                                                                                                                            gTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGaa         >  1:238204/1‑251 (MQ=255)                                                                                                                                                                                               ataCCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAAttt      >  1:467549/1‑250 (MQ=255)                                                                                                                                                                                                   cGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGc                                                                                                >  1:546976/1‑157 (MQ=255)                                                                                                                                                                                                    gTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCa                                                                                                                 >  1:641554/1‑139 (MQ=255)                                                                                                                                                                                                    gTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAAt  >  1:58008/1‑250 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                     GCTCCGAACAGCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTCGAACTCTTCCTGCCGCAAACAGTAAGCTTCCACTGTGGTTGCTCGCAAGAACGTTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAAT  >  4/6853‑7297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑