Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	4	7,070	C→G	V222V (GTC→GTG)	unknown →	33 kDa chaperonin HslO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	4	7,070	0	C	G	100.0%	155.2 / NA	59	V222V (GTC→GTG)	unknown	33 kDa chaperonin HslO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (55/4);  total (55/4)

CAGCGAATGTTTGGAAGATTATTTCATGCGCTCCGAACAGCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTCGAACTCTTCCTGCCGCAAACAGTAAGCTTCCACTGTGGTTGCTCGCAAGAACGTTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAAT  >  4/6824‑7297                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                    aacaatagccagggttgATTATTTCATGCGCTCCGAACAGCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGaa                                                                                                                                                                                                                                  <  1:888777/234‑1 (MQ=255)    cGAATGTTTGGAAGATTATTTCATGCGCTCCGAACAGCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAAc                                                                                                                                                                                                                                 <  1:451/248‑1 (MQ=255)    cGAATGTTTGGAAGATTATTTCATGCGCTCCGAACAGCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAAc                                                                                                                                                                                                                                 <  1:375342/248‑1 (MQ=255)        tGTTTGGAAGATTATTTCATGCGCTCCGAACAGCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTcc                                                                                                                                                                                                                           >  1:327771/1‑250 (MQ=255)          tttGGAAGATTATTTCATGCGCTCCGAACAGCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTcccg                                                                                                                                                                                                                         >  1:398352/1‑248 (MQ=255)                              gcTCCGAACAGCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGCTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCCAGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGCtttca                                                                                                                                                                                                     >  1:646568/1‑247 (MQ=255)                                 ccGAACAGCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGGTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACaagcgggaaggtttcactg                                                                                                                                                                                                  >  1:901760/1‑233 (MQ=255)                                   gAACAGCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACtgcgg                                                                                                                                                                                               >  1:149220/1‑248 (MQ=255)                                      cAGCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGcc                                                                                                                                                                                                                       <  1:468046/224‑1 (MQ=255)                                      cAGCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTg                                                                                                                                                                                            >  1:189749/1‑251 (MQ=255)                                        gCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACaagcggta                                                                                                                                                                                                             >  1:490698/1‑226 (MQ=255)                                        gCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACaagc                                                                                                                                                                                                                 >  1:45500/1‑226 (MQ=255)                                        gCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTg                                                                                                                                                                                            >  1:724829/1‑249 (MQ=255)                                        gCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTg                                                                                                                                                                                            >  1:626191/1‑249 (MQ=255)                                        gCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGAAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTg                                                                                                                                                                                            >  1:427845/1‑249 (MQ=255)                                                    gCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCCCAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACgc                                                                                                                                                                               >  1:289226/1‑249 (MQ=255)                                                     cTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACt                                                                                                                                                                                                   >  1:108758/1‑229 (MQ=255)                                                          catTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCgggg                                                                                                                                                                        >  1:31606/1‑249 (MQ=255)                                                                cACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCcgcg                                                                                                                                                                   >  1:449607/1‑250 (MQ=255)                                                                    ggCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAAc                                                                                                                                                                                                                                 >  1:690463/1‑184 (MQ=255)                                                                              aCGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTc                                                                                                                                                     >  1:490088/1‑250 (MQ=255)                                                                                        ggTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATAACGTTTATATCACGAAGAAAGCGTGGAAATCTTCCCGCCACAAGCGGTAAGTTTTCAATGCGGTTGCTCGCAAAAACGCTCCGGCGCCGCGCTGTTATTGAATTCCGAAGGAGaaa                                                                                                                                          >  1:663190/1‑251 (MQ=255)                                                                                         gTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAAt                                                                                                                                         >  1:518737/1‑251 (MQ=255)                                                                                             gcgcGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGGAAGTTTTCACTGCGGTTTCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGaa                                                                                                                                     >  1:381989/1‑251 (MQ=255)                                                                                                  ggCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAAt                                                                                                                                >  1:682309/1‑251 (MQ=255)                                                                                                  ggCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAAt                                                                                                                                >  1:777509/1‑251 (MQ=255)                                                                                                                              gttccggttcACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGat                                                                                                    >  1:8157/1‑251 (MQ=255)                                                                                                                                        aCCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCg                                                                                          >  1:828565/1‑251 (MQ=255)                                                                                                                                         ccGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAgttttcac                                                                                                                                                                                                    >  1:524866/1‑137 (MQ=255)                                                                                                                                         ccGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGc                                                                                                             >  1:245482/1‑231 (MQ=255)                                                                                                                                         ccGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGc                                                                                                >  1:838897/1‑244 (MQ=255)                                                                                                                                         ccGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGa                                                                                         >  1:834870/1‑251 (MQ=255)                                                                                                                                                                  cGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGACGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGtt                                                                 >  1:434637/1‑250 (MQ=255)                                                                                                                                                                  cGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGtt                                                                 >  1:210798/1‑250 (MQ=255)                                                                                                                                                                      aaCGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTc                                                                >  1:37248/1‑247 (MQ=255)                                                                                                                                                                      aaCGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATa                                                            >  1:370980/1‑251 (MQ=255)                                                                                                                                                                      aaCGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATa                                                            >  1:494499/1‑251 (MQ=255)                                                                                                                                                                        cGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGc                                                                                                                          >  1:19343/1‑187 (MQ=255)                                                                                                                                                                              ccATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAgttttcac                                                                                                                                                                                                    >  1:753301/1‑100 (MQ=255)                                                                                                                                                                              ccATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGAt                                                                                                                                                        >  1:612844/1‑151 (MQ=255)                                                                                                                                                                              ccATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGc                                                                                                                          >  1:675847/1‑181 (MQ=255)                                                                                                                                                                              ccATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCg                                                    >  1:3216/1‑251 (MQ=255)                                                                                                                                                                              ccATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCg                                                    >  1:193732/1‑251 (MQ=255)                                                                                                                                                                               cATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTg                                                                   >  1:777609/1‑235 (MQ=255)                                                                                                                                                                                                      gTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCgg                              >  1:11487/1‑249 (MQ=255)                                                                                                                                                                                                        ttAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTg                                                                   >  1:104653/1‑210 (MQ=255)                                                                                                                                                                                                                    gaaTTGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACg              >  1:649633/1‑251 (MQ=255)                                                                                                                                                                                                                       ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAAtgc                                                                                     >  1:140040/1‑177 (MQ=255)                                                                                                                                                                                                                       ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCattatt                                                                     >  1:587459/1‑193 (MQ=255)                                                                                                                                                                                                                       ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAgg                                                      >  1:218447/1‑208 (MQ=255)                                                                                                                                                                                                                       ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCg           >  1:726706/1‑251 (MQ=255)                                                                                                                                                                                                                       ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCg           >  1:659612/1‑251 (MQ=255)                                                                                                                                                                                                                       ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCg           >  1:14724/1‑251 (MQ=255)                                                                                                                                                                                                                       ttGTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATACGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCg           >  1:433478/1‑251 (MQ=255)                                                                                                                                                                                                                         gTTATACCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGaa         >  1:238204/1‑251 (MQ=255)                                                                                                                                                                                                                            ataCCGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAAttt      >  1:467549/1‑250 (MQ=255)                                                                                                                                                                                                                                cGTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGc                                                                                                >  1:546976/1‑157 (MQ=255)                                                                                                                                                                                                                                 gTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCa                                                                                                                 >  1:641554/1‑139 (MQ=255)                                                                                                                                                                                                                                 gTTTATATCACGAAGAAAGCGTGGAACTCTTCCCGCCACAAGCGGTAAGTTTTCACTGCGGTTGCTCGCAAGAACGCTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAAt  >  1:58008/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                    CAGCGAATGTTTGGAAGATTATTTCATGCGCTCCGAACAGCTACAAACCCAGCTCATCATTCGCACCGGCGAATATGACGGCAGACCGGTCAGCGCGGGCGTGTTGTTGCAAATCGTGCCGGACGGTTCCGGTTCACCGGAAGATTTCGAACATTTGGCAACGCTAACGGCAACCATTAAAGAAGAAGAAATTTTCGGTTTAACGGCGGAAGAATTGTTATACCGTTTATATCACGAAGAAAGCGTCGAACTCTTCCTGCCGCAAACAGTAAGCTTCCACTGTGGTTGCTCGCAAGAACGTTCCGGCGCCGCGCTGTTATTGATTTCCGAAGGAGAAATCGAACAAATTCTGGCAGAACACCAAGGCACCATCGATATGCAATGCGAATGCTGCGGCACTCATTATTTGTTCAATAAAGAGGCGATTGAGAAGTTGAAGCAATCGGCTTCTTAAGGTTAACGCCGAATTCTAAT  >  4/6824‑7297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑