Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	4	2,295	C→T	V125V (GTG→GTA)	unknown ←	Predicted membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	4	2,295	0	C	T	100.0%	175.9 / NA	64	V125V (GTG→GTA)	unknown	Predicted membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/45);  total (19/45)

CCCGCGCCCAATAAGGCTAAAGAAAATCCCATCGGTACCTGAAAGACGGCTTTGCCACCAAATCCGGTGAACCAGCCGCTGGCGCTATCTGCCATCACGCGCAGACCATTGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAACAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTCACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAGTGTCCCCGCAGAAGAGGCTTGGGTTTGCACCATATTATTTTCTAAAATACTGGAATCCTTGTAGAATTTCAGTACCGCCATGGAAATCACGGCTG  >  4/2050‑2536                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  cccGCGCCCAATAAGGCTAAAGAAAATCCCATCGGTACCTGAAAGACGGCTTTGCCACCAAATCCGGTGAACCAGCCGCTGGCGCTATCTGCCATCACGCGCAGACCATTGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTtactac                                                                                                                                                                                                                                              <  1:851837/251‑1 (MQ=255) cccGCGCCCAATAAGGCTAAAGAAAATCCCATCGGTACCTGAAAGACGGCTTTGCCACCAAATCCGGTGAACCAGCCGCTGGCGCTATCTGCCATCACGCGCAGACCATTGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTtactac                                                                                                                                                                                                                                              <  1:787535/251‑1 (MQ=255) cccGCGCCCAATAAGGCTAAAGAAAATCCCATCGGTACCTGAAAGACGGCTTTGCCACCAAATCCGGTGAACCAGCCGCTGGCGCTATCTGCCATCACGCGCAGACCATTGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTtactac                                                                                                                                                                                                                                              <  1:402488/251‑1 (MQ=255)   cgcgCCCAATAAGGCTAAAGAAAATCCCATCGGTCCCTGAAAGACGGCTTTGCCACCAAATCCGGTGAACCAGCCGCTGGCGCTATCTGCCATCACGCGCAGACCATTGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCa                                                                                                                                                                                                                                            <  1:36652/251‑1 (MQ=255)         cAATAAGGCTAAAGAAAATCCCATCGGTACCTGAAAGACGGCTTTGCCACCAAATCCGGTGAACCAGCCGCTGGCGCTATCTGCCATCACGCGCAGACCATTGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCac                                                                                                                                                                                                                                      <  1:489752/251‑1 (MQ=255)         cAATAAGGCTAAAGAAAATCCCATCGGTACCTGAAAGACGGCTTTGCCACCAAATCCGGTGAACCAGCCGCTGGCGCTATCTGCCATCACGCGCAGACCATTGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCa                                                                                                                                                                                                                                       >  1:615888/1‑250 (MQ=255)               ggCTAAAGAAAATCCCATCGGTACCTGAAAGACGGCTTTGCCACCAAATCCGGTGAACCAGCCGCTGGCGCTATCTGCCATCACGCGCAGACCATTGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACt                                                                                                                                                                                                                                                <  1:499498/235‑1 (MQ=255)               ggCTAAAGAAAATCCCATCGGTACCTGAAAGACGGCTTTGCCACCAAATCCGGTGAACCAGCCGCTGGCGCTATCTGCCATCACGCGCAGACCATTGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTa                                                                                                                                                                                                                                >  1:267874/1‑251 (MQ=255)                   aaaGAAAATCCCATCGGTACCTGAAAGACGGCTTTGCCACCAAATCCGGTGAACCAGCCGCTGGCGCTATCTGCCATCACGCGCAGACCATTGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTTACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACgg                                                                                                                                                                                                                            <  1:9226/251‑1 (MQ=255)                   aaaGAAAATCCCATCGGTACCTGAAAGACGGCTTTGCCACCAAATCCGGTGAACCAGCCGCTGGCGCTATCTGCCATCACGCGCAGACCATTGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACgg                                                                                                                                                                                                                            <  1:47308/251‑1 (MQ=255)                     aGAAAATCCCATCGGTACCTGAAAGACGGCTTTGCCCCCAAATCCGGTGAACCAGCCGCTGGCGCTATCTGCCATCACGCGCAGACCATTGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTtactac                                                                                                                                                                                                                                              <  1:808192/231‑1 (MQ=255)                      gAAAATCCCATCGGTACCTGAAAGACGGCTTTGCCACCAAATCCGGTGAACCAGCCGCTGGCGCTATCTGCCATCACGCGCAGACCATTGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTtactac                                                                                                                                                                                                                                              <  1:459893/230‑1 (MQ=255)                       aaaaTCCCATCGGTACCTGAAAGACGGCTTTGCCACCAAATCCGGTGAACCAGCCGCTGGCGCTATCTGCCATCACGCGCAGACCATTGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAAt                                                                                                                                                                                                                         <  1:483721/250‑1 (MQ=255)                               aTCGGTACCTGAAAGACGGCTTTGCCACCAAATCCGGTGAACCAGCCGCTGGCGCTATCTGCCATCACGCGCAGACCATTGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAaataa                                                                                                                                                                                                                <  1:75235/251‑1 (MQ=255)                                tCGGTACCTGAAAGACGGCTTTGCCACCAAATCCGGTGAACCAGCCGCTGGCGCTATCTGCCATCACGCGCAGACCATTGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAaataat                                                                                                                                                                                                               <  1:219105/251‑1 (MQ=255)                                     aCCTGAAAGACGGCTTTGCCACCAAATCCGGTGAACCAGCCGCTGGCGCTATCTGCCATCACGCGCAGACCATTGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCa                                                                                                                                                                                                                                            <  1:81568/217‑1 (MQ=255)                                       cTGAAAGACGGCTTTGCCACCAAATCCGGTGAACCAGCCGCTGGCGCTATCTGCCATCACGCGCAGACCATTGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCa                                                                                                                                                                                                        >  1:263763/1‑251 (MQ=255)                                              aCGGCTTTGCCACCAAATCCGGTGAACCAGCCGCTGGCGCTATCTGCCATCACGCGCAGACCATTGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTAc                                                                                                                                                                                                  <  1:663616/250‑1 (MQ=255)                                                        caccaAATCCGGTGAACCAGCCGCTGGCGCTATCTGCCATCACGCGCAGACCATTGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTAc                                                                                                                                                                                                  >  1:646455/1‑240 (MQ=255)                                                         accaAATCCGGTGAACCAGCCGCTGGCGCTATCTGCCATCACGCGCAGACCATTGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATgg                                                                                                                                                                                                                                         <  1:208438/200‑1 (MQ=255)                                                                 cGGTGAACCAGCCGCTGGCGCTATCTGCCATCACGCGCAGACCATTGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAAc                                                                                                                                                                              <  1:551570/251‑1 (MQ=255)                                                                                     cTATCTGCCATCACGCGCAGACCATTGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACgg                                                                                                                                                                                                                            <  1:685927/185‑1 (MQ=255)                                                                                       aTCTGCCATCACGCGCAGACCATTGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTaa                                                                                                                                                                                                                    <  1:239733/191‑1 (MQ=255)                                                                                           gCCATCACGCGCAGACCATTGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTAc                                                                                                                                                                                                  <  1:854302/205‑1 (MQ=255)                                                                                                cACGCGCAGACCATTGGTTAAAACCGCCACTATCCTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAAt                                                                                                                                               <  1:790664/251‑1 (MQ=255)                                                                                                    cgcAGACCATTGGTTAAAAACGCCCCTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGc                                                                                                                                            <  1:175131/250‑1 (MQ=255)                                                                                                      cAGACCATTGGTTAAAAACCCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGccc                                                                                                                                          <  1:782067/250‑1 (MQ=255)                                                                                                        gACCATTGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTtactac                                                                                                                                                                                                                                              <  1:117593/148‑1 (MQ=255)                                                                                                              tGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAAc                                                                                                                                 <  1:740936/251‑1 (MQ=255)                                                                                                               ggTTAAAACCGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGc                                                                                                                                            <  1:771484/239‑1 (MQ=255)                                                                                                               ggTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACgg                                                                                                                                                                                                                            <  1:492077/159‑1 (MQ=255)                                                                                                                 ttAAAAACGCCACTATACTCGCCAGCCCGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACaa                                                                                                                               <  1:258187/250‑1 (MQ=255)                                                                                                                        cGCCACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTAATTACCGGCTTTTAAAATTTCTGCCGCTGCCACGCCCTCAGGATAAGGCAAATCACTGTTTACTACCATgg                                                                                                                                                                                                                                         <  1:661401/137‑1 (MQ=255)                                                                                                                          ccACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACgg                                                                                                                                                                                                                            <  1:102883/148‑1 (MQ=255)                                                                                                                          ccACTATACTCGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGt                                                                                                                                                                                                    <  1:102712/172‑1 (MQ=255)                                                                                                                                    cGCCAGCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACCCCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATAccc                                                                                                                                                                                                           <  1:519118/155‑1 (MQ=255)                                                                                                                                         gCACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTtactac                                                                                                                                                                                                                                              <  1:302083/115‑1 (MQ=255)                                                                                                                                          cACGCCGCCGTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGgaag                                                                                                     <  1:891118/251‑1 (MQ=255)                                                                                                                                               cgccgTAAAAAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAgt                                                                                                <  1:468676/251‑1 (MQ=255)                                                                                                                                                               tttCACCCCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAGTGTCCCCGCAGAAGAgg                                                                                <  1:164095/251‑1 (MQ=255)                                                                                                                                                                 tCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAGTGTCCCCGCAGAAGAGGCt                                                                              <  1:369480/251‑1 (MQ=255)                                                                                                                                                                 tCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAGTGTCCCCGCAGAAGAGGCt                                                                              <  1:264227/251‑1 (MQ=255)                                                                                                                                                                     cccGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAGTGTCCCCGCAGAAGAGGCTTggg                                                                          <  1:582744/250‑1 (MQ=255)                                                                                                                                                                      ccGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAaataat                                                                                                                                                                                                               <  1:749262/117‑1 (MQ=255)                                                                                                                                                                           gTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATgg                                                                                                                                                                                                                                         <  1:277131/86‑1 (MQ=255)                                                                                                                                                                           gTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTaaa                                                                                                                                                                                                                   <  1:703506/108‑1 (MQ=255)                                                                                                                                                                                 aTCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAGTGTCCCCGCAGAAGAGGCTTGGGTTTGCACCatat                                                              <  1:722974/251‑1 (MQ=255)                                                                                                                                                                                      tgtgATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAGTGTCCCCGCAGAAGAGGCTTGGGTTTGCACCAtattat                                                           <  1:1407/249‑1 (MQ=255)                                                                                                                                                                                      tgtgATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAGTGTCCCCGCAGAAGAGGCTTGGGTTTGCACCAtattat                                                           <  1:471003/249‑1 (MQ=255)                                                                                                                                                                                      tgtgATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAGTGTCCCCGCAGAAGAGGCTTGGGTTTGCACCATATTAttt                                                         <  1:103713/251‑1 (MQ=255)                                                                                                                                                                                      tgtgATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAGTGTCCCCGCAGAAGAGGCTTGGGTTTGCACCATATTAttt                                                         <  1:346084/251‑1 (MQ=255)                                                                                                                                                                                                       aaaaTTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAGTGTCCCCGCAGAAGAGGCTGGGGTTTGCACCATATTATTTTCTAAAATACTGGAATc                                        <  1:843752/251‑1 (MQ=255)                                                                                                                                                                                                                      gCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAGTGTCCCCGCAGAAGAGGCt                                                                              >  1:892018/1‑198 (MQ=255)                                                                                                                                                                                                                      gCCACACCCTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAGTGTCCCCGCAGAAGAGGCTTGGGTTTGCACCATATTAttt                                                         <  1:558388/219‑1 (MQ=255)                                                                                                                                                                                                                            cccTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGga                                                                                                       >  1:455108/1‑167 (MQ=255)                                                                                                                                                                                                                            cccTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAGTGTCCCCGCAGAAGAGGCTTGGGTTTGCACCATATTATTTTCTAAAATACTGGAATCCTTGTAGAATTTCAGTACCg                    >  1:267894/1‑250 (MQ=255)                                                                                                                                                                                                                            cccTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAGTGTCCCCGCAGAAGAGGCTTGGGTTTGCACCATATTATTTTCTAAAATACTGGAATCCTTGTAGAATTTCAGTACCg                    >  1:721109/1‑250 (MQ=255)                                                                                                                                                                                                                            cccTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAGTGTCCCCGCAGAAGAGGCTTGGGTTTGCACCATATTATTTTCTAAAATACTGGAATCCTTGTAGAATTTCAGTACCg                    >  1:137121/1‑250 (MQ=255)                                                                                                                                                                                                                              cTCAGGATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAGTGTCCCCGCAGAAGAGGCTTGGGTTTGCAc                                                                   >  1:371438/1‑201 (MQ=255)                                                                                                                                                                                                                                  ggATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTaa                                                                                                                                                                                                                    >  1:555902/1‑52 (MQ=255)                                                                                                                                                                                                                                  ggATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTaa                                                                                                                                                                                                                    >  1:538127/1‑52 (MQ=255)                                                                                                                                                                                                                                  ggATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAaataat                                                                                                                                                                                                               >  1:136564/1‑57 (MQ=255)                                                                                                                                                                                                                                  ggATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAgt                                                                                                <  1:711521/168‑1 (MQ=255)                                                                                                                                                                                                                                  ggATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAGTGTCCCCGCAGAAGAGGCTTGGGTTTGCACCATATTAttt                                                         >  1:428617/1‑207 (MQ=255)                                                                                                                                                                                                                                  ggATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAGTGTCCCCGCAGAAGAGGCTTGGGTTTGCACCATATTATTTTCTAAAATACTGGAATCCTTGTAGAATTTCAGTACCGCCATGGa             >  1:429606/1‑251 (MQ=255)                                                                                                                                                                                                                                  ggATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAGTGTCCCCGCAGAAGAGGCTTGGGTTTGCACCATATTATTTTCTAAAATACTGGAATCCTTGTAGAATTTCAGTACCGCCATGGa             >  1:440453/1‑251 (MQ=255)                                                                                                                                                                                                                                  ggATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAGTGTCCCCGCAGAAGAGGCTTGGGTTTGCACCATATTATTTTCTAAAATACTGGAATCCTTGTAGAATTTCAGTACCGCCATGGa             >  1:236461/1‑251 (MQ=255)                                                                                                                                                                                                                                  ggATAAGGCAAATCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACAGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAGTGTCCCCGCAGAAGAGGCTTGGGTTTGCACCATATTAttt                                                         >  1:402755/1‑207 (MQ=255)                                                                                                                                                                                                                                    atagggaaaaTCACTTTTTGCTACCATGGCACAACGTAACGAAATAGTAAATAATACCCCCAAGGAACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAGTGTCCCCGCAGAAGAGGCTTGGGTTTGCACCATATTATTTTCTAAAATACTGGAATCCTTGTAGAATTTCAGTACCGCCATGGaa            <  1:360740/243‑1 (MQ=255)                                                                                                                                                                                                                                              tCACTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAGTGTCCCCGCAGAAGAGGCTTGGGTTTGCACCATATTATTTTCTAAAATACTGGAATCCTTGTAGAATTTCAGTACCGCCATGGAAATCACGGCTg  >  1:413440/1‑250 (MQ=255)                                                                                                                                                                                                                                                 cTGTTTACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAg                                                                                                 <  1:692514/152‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  CCCGCGCCCAATAAGGCTAAAGAAAATCCCATCGGTACCTGAAAGACGGCTTTGCCACCAAATCCGGTGAACCAGCCGCTGGCGCTATCTGCCATCACGCGCAGACCATTGGTTAAAAACGCCACTATACTCGCCAGCACGCCGCCGTAAACAATGTCTTTCACACCGCTGTCACCATCAGTGTGATTACCGGCTTTTAAAATTTCTGCCGCTGCCACACCCTCAGGATAAGGCAAATCACTGTTCACTACCATGGCACGACGTAACGGAATAGTAAATAATACCCCCAAAGTACCGCCCGCCGCACAAATCAACATGGTTTGCCAAAACGGAAAATCCTGCCAATAGCCCATCATTAACAAACCCGGCAATACGAAAATCACGGAAGAAAGTGTCCCCGCAGAAGAGGCTTGGGTTTGCACCATATTATTTTCTAAAATACTGGAATCCTTGTAGAATTTCAGTACCGCCATGGAAATCACGGCTG  >  4/2050‑2536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑