Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	4	1,587	T→C	A361A (GCA→GCG)	unknown ←	Predicted membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	4	1,587	0	T	C	98.6%	185.5 / NA	70	A361A (GCA→GCG)	unknown	Predicted membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (20/49);  total (20/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTCCACCAACAAGCCGGTTTTCAAATCCTGCAGGTTGTCGTTGGAAATGGTTGCTGTGGTGAGCACGATGGAAGCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGACGCCCCTACAAGCCCGGCCATGTAACCGGATGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAGTGATGTAAAGAAATCACAATCAAGATAACAGTCGCGATCAAAATATAAATCATGGTTTTCGGCGATAGGTCAATATCAATACGATGTTCGTATTCTGCCTGATGTCCCTTCAACATACGGAAAGAATGCGCCATACCTTGTAC  >  4/1342‑1829                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   gCTTCCACCAACAAGCCGGTTTTCAAATCCTGCAGGTTGTCGTTGGAAATGGTTGCTGTGGTGAGCACGATGGAAGCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGAcgctg                                                                                                                                                                                                                                                >  1:486802/1‑250 (MQ=255)                ccGGTTTTCAAATCCTGCAGGTTGTCGTTGGAAATGGTTGCTGTGGTGAGCACGATGGAAGCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAAc                                                                                                                                                                                                                                 <  1:267325/250‑1 (MQ=255)                             ccTGCAGGTTGTCGTTGGAAATGGTTGCTGTGGTGAGCACGATGGAAGCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCa                                                                                                                                                                                                                   >  1:84932/1‑251 (MQ=255)                                   ggTTGTCGTTGGAAATGGTTGCTGTGGTGAGCACGATGGAAGCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAAt                                                                                                                                                                                                             >  1:190657/1‑251 (MQ=255)                                        tCGTTGGAAATGGTTGCTGTGGTGAGCACGATGGAAGCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTAc                                                                                                                                                                                                         <  1:164784/250‑1 (MQ=255)                                        tCGTTGGAAATGGTTGCTGTGGTGAGCACGATGGAAGCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTAc                                                                                                                                                                                                         <  1:483732/250‑1 (MQ=255)                                         cGTTGGAAATGGTTGCTGTGGTGAGCACGATGGAAGCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACaa                                                                                                                                                                                                       >  1:256072/1‑251 (MQ=255)                                            tGGAAATGGTTGCTGTGGTGAGCACGATGGAAGCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAacc                                                                                                                                                                                                    <  1:584704/251‑1 (MQ=255)                                            tGGAAATGGTTGCTGTGGTGAGCACGATGGAAGCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAacc                                                                                                                                                                                                    <  1:881216/251‑1 (MQ=255)                                                 aTGGTTGCTGTGGTGAGCACGATGGAAGCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAaccaccac                                                                                                                                                                                               <  1:415446/251‑1 (MQ=255)                                                     ttGCTGTGGTGAGCACGATGGAAGCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAacc                                                                                                                                                                                                    <  1:637382/242‑1 (MQ=255)                                                      tGCTGTGGTGAGCACGATGGAAGCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATa                                                                                                                                                                                          <  1:806741/251‑1 (MQ=255)                                                      tGCTGTGGTGAGCACGATGGAAGCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATa                                                                                                                                                                                          <  1:330189/251‑1 (MQ=255)                                                       gCTGTGGTGAGCACGATGGAAGCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCTCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAacc                                                                                                                                                                                                    <  1:682568/240‑1 (MQ=255)                                                       gCTGTGGTGAGCACGATGGAAGCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAAc                                                                                                                                                                                                                       <  1:354235/221‑1 (MQ=255)                                                          gtggtgAGCACGATGGAAGCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATaaaa                                                                                                                                                                                                                         <  1:645144/216‑1 (MQ=255)                                                           tggtgAGCACGATGGAAGCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAagag                                                                                                                                                                                      <  1:138565/250‑1 (MQ=255)                                                           tggtgAGCACGATGGAAGCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAagag                                                                                                                                                                                      <  1:660792/250‑1 (MQ=255)                                                            ggtgAGCACGATGGAAGCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAag                                                                                                                                                                                        <  1:695602/247‑1 (MQ=255)                                                            ggtgAGCACGATGGAAGCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCCATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAgtg                                                                                                                                                                                    >  1:582714/1‑251 (MQ=255)                                                              tgAGCACGATGGAAGCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAgtgt                                                                                                                                                                                   <  1:868192/250‑1 (MQ=255)                                                                   aCGATGGAAGCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTc                                                                                                                                                                             <  1:781818/251‑1 (MQ=255)                                                                    cGATGGAAGCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAacc                                                                                                                                                                                                    >  1:707118/1‑227 (MQ=255)                                                                    cGATGGAAGCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCa                                                                                                                                                                            >  1:46182/1‑251 (MQ=255)                                                                         gAAGCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTg                                                                                                                                                                        <  1:868166/250‑1 (MQ=255)                                                                            gCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAagag                                                                                                                                                                                      >  1:374893/1‑233 (MQ=255)                                                                             cGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAAt                                                                                                                                                                    >  1:583124/1‑250 (MQ=255)                                                                             cGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAAt                                                                                                                                                                    >  1:286/1‑250 (MQ=255)                                                                             cGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATc                                                                                                                                                                   <  1:509520/251‑1 (MQ=255)                                                                               gTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAaccaccac                                                                                                                                                                                               <  1:724755/221‑1 (MQ=255)                                                                                 gAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTg                                                                                                                                                               >  1:559563/1‑251 (MQ=255)                                                                                 gAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTg                                                                                                                                                               >  1:117671/1‑251 (MQ=255)                                                                                 gAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTg                                                                                                                                                               >  1:706413/1‑251 (MQ=255)                                                                                      aGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAagag                                                                                                                                                                                      >  1:118085/1‑223 (MQ=255)                                                                                       ggTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAg                                                                                                                                                         >  1:824811/1‑251 (MQ=255)                                                                                       ggTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAg                                                                                                                                                         >  1:682004/1‑251 (MQ=255)                                                                                               cGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAg                                                                                                                                                 <  1:638921/251‑1 (MQ=255)                                                                                                              gTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTa                                                                                                                                                                                                                                          >  1:882114/1‑147 (MQ=255)                                                                                                                ccATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAGTGATGTAAAGAAATcac                                                                                                                                <  1:215345/251‑1 (MQ=255)                                                                                                                    cGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAagag                                                                                                                                                                                      <  1:660813/193‑1 (MQ=255)                                                                                                                    cGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAGTGATGTAAAGAAATCACAATc                                                                                                                            <  1:473961/251‑1 (MQ=255)                                                                                                                         gtTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAGTGATGTAAAGAAATCACAATCAAGa                                                                                                                        <  1:393164/250‑1 (MQ=255)                                                                                                                         gtTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAGTGATGTAAAGAAATCACAATCAAGAt                                                                                                                       >  1:579067/1‑251 (MQ=255)                                                                                                                           ttCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAagag                                                                                                                                                                                      >  1:484920/1‑186 (MQ=255)                                                                                                                            tCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAGTGATGTAAAGAAATCACAATCAAGATAAc                                                                                                                    <  1:761527/251‑1 (MQ=255)                                                                                                                              aaaTAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAGTGATGTAAAGAAATCACAATCAAGATAACAg                                                                                                                  <  1:822086/251‑1 (MQ=255)                                                                                                                                            cATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTAc                                                                                                                                                                                                         <  1:663552/150‑1 (MQ=255)                                                                                                                                            cATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAGTGATGTAAAGAAATCACAATCAAGATAACAGTCGCGATCAAAata                                                                                                    <  1:528561/251‑1 (MQ=255)                                                                                                                                                  cGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAGTGATGTAAAGAAATCACAATCAAGATAACAGTCGCGATCAAAATATAAATc                                                                                              <  1:865477/251‑1 (MQ=255)                                                                                                                                                    aTAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTc                                                                                                                                                                             <  1:37506/170‑1 (MQ=255)                                                                                                                                                       tgtatagagacagaaGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAGTGATGTAAAGAAATCACAATCAAGATAACAGTCGCGATCAAAATATAAATCATGGt                                                                                         <  1:834400/238‑1 (MQ=255)                                                                                                                                                         aaCCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAAGCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAagag                                                                                                                                                                                      <  1:492782/156‑1 (MQ=255)                                                                                                                                                             aaCACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAGTGATGTAAAGAAATCACAATCAAGATAACAGTCGCGATCAAAATATAAATCATGGTTTTCgg                                                                                   <  1:747084/251‑1 (MQ=255)                                                                                                                                                               cacCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCAGTGCGGCAGCGATAAAGTGATGTAAAGAAATCACAATCAAGATAACAGTCGCGATCAAAATATaaa                                                                                                >  1:435955/1‑236 (MQ=255)                                                                                                                                                                           cgcgATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAGTGATGTAAAGAAATCACAATCAAGATAACAGTCGCGATCAAAATATAAATCATGGTTTTCGGCGATAGGtcaatat                                                                     <  1:265304/251‑1 (MQ=255)                                                                                                                                                                             cgATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAGTGATGTAAAGAAATCACAATCAAGATAACAGTCGCGATCAAAATATAAATCATGGTTTTCGGCGATAGGtcaatatc                                                                    <  1:35021/250‑1 (MQ=255)                                                                                                                                                                             cgATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAGTGATGTAAAGAAATCACAATCAAGATAACAGTCGCGATCAAAATATAAATCATGGTTTTCGGCGATAGGtcaatatc                                                                    <  1:301929/250‑1 (MQ=255)                                                                                                                                                                                 gACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAAtt                                                                                                                                                                              <  1:75168/140‑1 (MQ=255)                                                                                                                                                                                   cGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAGTGATGTAAAGAAATCACAATCAAGATAACAGTCGCGATCAAAATATAAATCATGGTTTTCGGCGATAGGTCAATATCAATACGa                                                             <  1:709593/251‑1 (MQ=255)                                                                                                                                                                                     gAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAGTGATGTAAAGAAATCACAATCAAGATAACAGTCGCGATCAAAATATAAATCATGGTTTTCGGCGATAGGtcaatatcaat                                                                 <  1:326560/245‑1 (MQ=255)                                                                                                                                                                                     gAAATAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAGTGATGTAAAGAAATCACAATCAAGATAACAGTCGCGATCAAAATATAAATCATGGTTTTCGGCGATAGGTCAATATCAATAc                                                               <  1:195809/247‑1 (MQ=255)                                                                                                                                                                                      aaaTAATCCCGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGATGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAGTGATGTAAAGAAATCACAATCAAGATAACAGTCGCGATCAAAATATAAATCATGGTTTTCGGCGATAGGt                                                                           <  1:426085/234‑1 (MQ=255)                                                                                                                                                                                             cccGATACCGGAAATCGGGCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAGTGATGTAAAGAAATCACAATCAAGATAACAGTCGCGATCAAAATATAAATCATGGTTTTCGGCGATAGGTCAATATCAATACGATGTTCGTAtt                                                   <  1:808991/251‑1 (MQ=255)                                                                                                                                                                                                              ggCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTc                                                                                                                                                                             <  1:585336/112‑1 (MQ=255)                                                                                                                                                                                                              ggCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAGTGATGTAAAGAAATCACAATCAAGATAACAGTCGCGATCAAAATATAAATCATGGTTTTCGGc                                                                                  <  1:391051/203‑1 (MQ=255)                                                                                                                                                                                                              ggCTGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAGTGATGTAAAGAAATCACAATCAAGATAACAGTCGCGATCAAAATATAAATCATGGTTTTCGGCGATAGGTCTATATCAATAc                                                               <  1:66310/222‑1 (MQ=255)                                                                                                                                                                                                                 tGGAAGAGGCCCCTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAGTGATGTAAAGAAATCACAATCAAGATAACAGTCGCGATCAAAATATAAATCATGGTTTTCGGCGATAGGTCAATATCAATACGATGTTCGTATTCTGCCTGATGTCCCTTCAAc                               <  1:179233/251‑1 (MQ=255)                                                                                                                                                                                                                             cTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAGTGATGTAAAGAAATCACAATCAAGATAACAGTCGCGATCAAAATGTAAATCATGGTTTTCGGCGATAGGTCAATATCAATACGATg                                                           >  1:436610/1‑211 (MQ=255)                                                                                                                                                                                                                             cTACAAGCCCGGCCATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAGTGATGTAAAGAAATCACAATCAAGATAACAGTCGCGATCAAAATATAAATCATGGTTTTCGGCGATAGGTCAATATCAATACGATGTTCGTATTCTGCCTGATGTCCCTTCAACATACGGAAAGaa                   <  1:12738/251‑1 (MQ=255)                                                                                                                                                                                                                                         ccATGTAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAGTGATGTAAAGAAATCACAATCAAGATAACAGTCGCGATCAAAATATAAATCATGGTTTTCGGCGATAGGTCAATATCAATACGATGTTCGTATTCTGCCTGATGTCCCTTCAACATACGGAAAGAATGCGCCATACCt       <  1:267394/251‑1 (MQ=255)                                                                                                                                                                                                                                              tAACCGGACGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAGTGATGTAAAGAAATCACAATCAAGATAACAGTCGCGATCAAAATATAAATCATGGTTTTCGGCGATAGGTCAATATCAATACGATGTTCGTATTCTGCCTGATGTCCCTTCAACATACGGAAAGAATGCGCCATACCTTGTAc  <  1:296503/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   GCTTCCACCAACAAGCCGGTTTTCAAATCCTGCAGGTTGTCGTTGGAAATGGTTGCTGTGGTGAGCACGATGGAAGCGGTGAACAAGGTCAGTGCGGTCAAAAATTTCTGTCCATCGGTGGTTTCAAATAAACCGGATGCATTACCGATAGAAACCAACACCAAAGAAATCGCGATGACGGAAATAATCCCGATACCGGAAATCGGGCTGGAAGACGCCCCTACAAGCCCGGCCATGTAACCGGATGCTGCCGCTATGAAAAAACCGATAAAAACTGCAAGGAATGTACAAACCACCACCAATAAGAGTGTTAATTCAGGTGAAATCGGTGCGGCAGCGATAAAGTGATGTAAAGAAATCACAATCAAGATAACAGTCGCGATCAAAATATAAATCATGGTTTTCGGCGATAGGTCAATATCAATACGATGTTCGTATTCTGCCTGATGTCCCTTCAACATACGGAAAGAATGCGCCATACCTTGTAC  >  4/1342‑1829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑