Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	3	102,201	G→A	G176G (GGC→GGT)	unknown ←	Methionine ABC transporter permease protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	3	102,201	0	G	A	100.0%	182.2 / NA	66	G176G (GGC→GGT)	unknown	Methionine ABC transporter permease protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/29);  total (37/29)

CCGTTAAGGCGAATACGGAGGCTAATGTTGCGACGCCTAATAATTTTTTCAAGTTCATAAAAGATCCCCTTTTTATGTCAGTGTGAAGTTAACTAACGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCGCCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAATTTCAACCAAGGCGTTGGCGGTTAAACGCGCCACAAACGGCATGGCACAAATACTCAGCGGAATAATGGCGGCAGTGGTG  >  3/101959‑102441                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 ccGTTAAGGCGAATACGGAGGCTAATGTTGCGACGCCTAATAATTTTTTCAAGTTCATAAAAGATCCCCTTTTTATGTCAATGTGAAGTTAACTAACGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGAt                                                                                                                                                                                                                                          >  1:541636/1‑251 (MQ=255) ccGTTAAGGCGAATACGGAGGCTAATGTTGCGACGCCTAATAATTTTTTCAAGTTCATAAAAGATCCCCTTTTTATGTCAATGTGAAGTTAACTAACGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGAt                                                                                                                                                                                                                                          >  1:736111/1‑251 (MQ=255) ccGTTAAGGCGAATACGGAGGCTAATGTTGCGACGCCTAATAATTTTTTCAAGTTCATAAAAGATCCCCTTTTTATGTCAATGTGAAGTTAACTAACGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGAt                                                                                                                                                                                                                                          >  1:74061/1‑251 (MQ=255)         gCGAATACGGAGGCTAATGTTGCGACGCCTAATAATTTTTTCAAGTTCATAAAAGATCCCCTTTTTATGTCAATGTGAAGTTAACTAACGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGc                                                                                                                                                                                                                                    <  1:345022/249‑1 (MQ=255)             aTACGGAGGCTAATGTTGCGACGCCTAATAATTTTTTCAAGTTCATAAAAGATCCCCTTTTTATGTCAATGTGAAGTTAACTAACGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATgg                                                                                                                                                                                                                               >  1:513538/1‑250 (MQ=255)              tACGGAGGCTAATGTTGCGACGCCTAATAATTTTTTCAAGTTCATAAAAGATCCCCTTTTTATGTCAATGTGAAGTTAACTAACGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCg                                                                                                                                                                                                                             <  1:462350/251‑1 (MQ=255)                    ggCTAATGTTGCGACGCCTAATAATTTTTTCAAGTTCATAAAAGATCCCCTTTTTATGTCAATGTGAAGTTAACTAACGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAg                                                                                                                                                                                                                       >  1:22772/1‑251 (MQ=255)                            ttGCGACGCCTAATAATTTTTTCAAGTTCATAAAAGATCCCCTTTTTATGTCAATGTGAAGTTAACTAACGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAg                                                                                                                                                                                                               <  1:351145/251‑1 (MQ=255)                                    ccTAATAATTTTTTCAAGTTCATAAAAGATCCCCTTTTTATGTCAATGTGAAGTTAACTAACGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGTCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCa                                                                                                                                                                                                       >  1:783082/1‑251 (MQ=255)                                    ccTAATAATTTTTTCAAGTTCATAAAAGATCCCCTTTTTATGTCAATGTGAAGTTAACTAACGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCa                                                                                                                                                                                                       >  1:317999/1‑251 (MQ=255)                                             tttttCAAGTTCATAAAAGATCCCCTTTTTATGTCAATGTGAAGTTAACTAACGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAggg                                                                                                                                                                                              <  1:459222/251‑1 (MQ=255)                                             tttttCAAGTTCATAAAAGATCCCCTTTTTATGTCAATGTGAAGTTAACTAACGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAggg                                                                                                                                                                                              <  1:350103/251‑1 (MQ=255)                                                     gTTCATAAAAGATCCCCTTTTTATGTCAATGTGAAGTTAACTAACGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAAt                                                                                                                                                                                                                         >  1:625370/1‑216 (MQ=255)                                                           aaaaGATCCCCTTTTTATGTCAATGTGAAGTTAACTAACGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATg                                                                                                                                                                                <  1:610897/251‑1 (MQ=255)                                                             aaGATCCCCTTTTTATGTCAATGTGAAGTTAACTAACGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAg                                                                                                                                                                              >  1:829554/1‑251 (MQ=255)                                                             aaGATCCCCTTTTTATGTCAATGTGAAGTTAACTAACGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAg                                                                                                                                                                              >  1:695731/1‑251 (MQ=255)                                                             aaGATCCCCTTTTTATGTCAATGTGAAGTTAACTAACGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAg                                                                                                                                                                              <  1:57707/251‑1 (MQ=255)                                                                     cTTTTTATGTCAATGTGAAGTTAACTAACGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATg                                                                                                                                                                                <  1:732337/241‑1 (MQ=255)                                                                     cTTTTTATGTCAATGTGAAGTTAACTAACGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGCAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGCCGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGCGATGCCGTTAATGAGTGTTGGCa                                                                                                                                                                      >  1:396917/1‑251 (MQ=255)                                                                      tttttATGTCAATGTGAAGTTAACTAACGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCaa                                                                                                                                                                     >  1:62905/1‑251 (MQ=255)                                                                          tATGTCAATGTGAAGTTAACTAACGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCGCCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCt                                                                                                                                                                 <  1:131792/251‑1 (MQ=255)                                                                            tGTCAATGTGAAGTTAACTAACGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCtt                                                                                                                                                                >  1:213332/1‑250 (MQ=255)                                                                                 atgtgAAGTTAACTAACGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCa                                                                                                                                                          >  1:330385/2‑251 (MQ=255)                                                                                     gAAGTTAACTAACGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAg                                                                                                                                                                              <  1:416600/227‑1 (MQ=255)                                                                                        gTTAACTAACGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGaa                                                                                                                                                   >  1:67774/1‑251 (MQ=255)                                                                                            actaacGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTa                                                                                                                                               >  1:635415/1‑251 (MQ=255)                                                                                                     gatCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATg                                                                                                                                                                                >  1:676620/1‑209 (MQ=255)                                                                                                             cGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAAtt                                                                                                                              >  1:288755/1‑251 (MQ=255)                                                                                                                  tGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATtggtgg                                                                                                                         <  1:312072/251‑1 (MQ=255)                                                                                                                   gCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATtggtgg                                                                                                                         >  1:219971/1‑250 (MQ=255)                                                                                                                        gTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCa                                                                                                                   >  1:288892/1‑251 (MQ=255)                                                                                                                                              aTCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTaa                                                                                             >  1:418676/1‑251 (MQ=255)                                                                                                                                                 aTGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGcc                                                                                          <  1:670164/251‑1 (MQ=255)                                                                                                                                                      gAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAggg                                                                                                                                                                                                             <  1:640251/131‑1 (MQ=255)                                                                                                                                                           ccACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAAtt                                                                                >  1:634621/1‑251 (MQ=255)                                                                                                                                                           ccACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAAtt                                                                                >  1:319462/1‑251 (MQ=255)                                                                                                                                                              caATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAATTTCa                                                                             >  1:68316/1‑251 (MQ=255)                                                                                                                                                                ataataatGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAATTTCaa                                                                            >  1:615693/1‑250 (MQ=255)                                                                                                                                                                    taatGGTGGCGACCCATTTCACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGAGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAATTTCAACCAAg                                                                       >  1:523171/1‑251 (MQ=255)                                                                                                                                                                                   aTTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTtgc                                                                                                         <  1:826016/202‑1 (MQ=255)                                                                                                                                                                                      ttACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAATTTCAACCAAGGCGTTGGCGGTTAAAcg                                                      >  1:139552/1‑250 (MQ=255)                                                                                                                                                                                        aCATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAggg                                                                                                                                                                                              >  1:138106/1‑112 (MQ=255)                                                                                                                                                                                             ggTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTtgc                                                                                                         <  1:214083/192‑1 (MQ=255)                                                                                                                                                                                              gTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAATTTCAACCAAGGCGTTGGCGGTTAAACGCGCCACaaa                                             >  1:355222/1‑251 (MQ=255)                                                                                                                                                                                                ccagttGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAATTTCAACCAAGGCGTTGGCGGTTAAACGCGCCACAAACg                                           <  1:640382/247‑1 (MQ=255)                                                                                                                                                                                                    ttGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAATTTCAACCAAGGCGTTGGCGGTTAAACGCGCCACAAACGGCAt                                       >  1:361214/1‑251 (MQ=255)                                                                                                                                                                                                      gCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGAcc                                                                                                                                                                                                        <  1:495420/88‑1 (MQ=255)                                                                                                                                                                                                         atatTCCCCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAATTTCAACCAAGGCGTTGGCGGTTAAACGCGCCACAAACGGCATGGcac                                  <  1:869361/251‑1 (MQ=255)                                                                                                                                                                                                         atatTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAATTTCAACCAAGGCGTTGGCGGTTAAACGCGCCACAAACGGCATGGcac                                  <  1:717889/251‑1 (MQ=255)                                                                                                                                                                                                           atTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGcc                                                                                          >  1:734832/1‑193 (MQ=255)                                                                                                                                                                                                             tCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAATTTCAACCAAGGCGTTGGCGGTTAAACGCGCCACAAACGGCATGGCACAAAt                              >  1:276889/1‑251 (MQ=255)                                                                                                                                                                                                               cccGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAATTTCAACCAAGGCGTTGGCGGTTAAACGCGCCACAAACGGCATGGCACAAATAc                            <  1:360472/250‑1 (MQ=255)                                                                                                                                                                                                               aCCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAATTTCAACCAAGGCGTTGGCGGTTAAACGCGCCACaaa                                             <  1:554621/234‑1 (MQ=255)                                                                                                                                                                                                                   tAGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAATTTCAACCAAGGCGTTGGCGGTTAAACGCGCCACAAACGGCATGGCACAAATAc                            >  1:91047/1‑247 (MQ=255)                                                                                                                                                                                                                    aGTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAATTTCAACCAAGGCGTTGGCgg                                                             >  1:390248/1‑213 (MQ=255)                                                                                                                                                                                                                     gTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAATTTCAACCAAGGCGTTGGCGGTTAAACGCGCCACAAACGGCATGGCACAAATACTCAGCg                      >  1:387105/1‑251 (MQ=255)                                                                                                                                                                                                                     gTTAATGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAATTTCAACCAAGGCGTTGGCGGTTAAACGCGCCACAAACGGCATGGCACAAATACTCAGCg                      >  1:233082/1‑251 (MQ=255)                                                                                                                                                                                                                         aTGGCAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAATTTCAACCAAGGCGTTGGCGGTTAAACGCGCCACAAACGGCATGGCACAAATACTCAGCGGaat                  <  1:166946/251‑1 (MQ=255)                                                                                                                                                                                                                             cAAGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAATTTCAACCAAGGCGTTGGCGGTTAAACGCGCCACAAACGGCATGGCACAAATACTCAGCGGaataat               <  1:586841/250‑1 (MQ=255)                                                                                                                                                                                                                              aaGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAATTTCAACCAAGGCGTTGGCGGTTAAACGCGCCACAAACGGCATGGCACAAATACTCAGCGGAATAATgg             <  1:563611/251‑1 (MQ=255)                                                                                                                                                                                                                              aaGGCTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAATTTCAACCAAGGCGTTGGCGGTTAAACGCGCCACAAACGGCATGGCACAAATACTCAGCGGAATAATgg             <  1:587053/251‑1 (MQ=255)                                                                                                                                                                                                                                  cTGCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAATTTCAACCAAGGCGTTGGCGGTTAAACGCGCCACAAACGGCATGGCACAAATACTCAGCGGAATAATggcggc         <  1:200529/251‑1 (MQ=255)                                                                                                                                                                                                                                   ggCCTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAATTTCAACCAAGGCGTTGGCGGTTAAACGCGCCACAAACGGCATGGCACAAATACTc                          <  1:694094/232‑1 (MQ=255)                                                                                                                                                                                                                                     ccTAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAATTTCAACCAAGGCGTTGGCGGTTAAACGCGCCACAAACGGCATGGCACAAATACTCAGCGGAATAATGGCGGCAgt      <  1:258801/251‑1 (MQ=255)                                                                                                                                                                                                                                       tAAGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAATTTCAACCAAGGCGTTGGCGGTTAAACGCGCCACAAACGGCATGGCACAAATACTCAGCGGAATAATGGCGGCAgtgg    <  1:276453/251‑1 (MQ=255)                                                                                                                                                                                                                                         aGCCACCGCCACCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAATTTCAACCAAGGCGTTGGCGGTTAAACGCGCCACAAACGGCATGGCACAAATACTCAGCGGAATAATGGCGGCAgtggtg  >  1:494189/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 CCGTTAAGGCGAATACGGAGGCTAATGTTGCGACGCCTAATAATTTTTTCAAGTTCATAAAAGATCCCCTTTTTATGTCAGTGTGAAGTTAACTAACGATGATCGACACGTTTTGCCAAGTCATCGCCAAGTTTTTGTGAAATCATGACGAATACCACAATAATAATGGTGGCGACCCATTTTACATAGGTCATATTGCGATATTCACCGTAGTTAATGGCAAGGCTGCCTAAGCCACCGCCGCCGACGATACCTGCCATGGCGGAATAGCCCACAAGGGTGACCAGCGTCAGGGTGATGCCGTTAATGAGTGTTGGCAAAGCTTCCGGCAAATAGAATTTACGGATAATCTGCCAATTGGTGGCGCCCATGGCTTTTGCTGCTTCCGTTAAGCCTTTTGGAATTTCAACCAAGGCGTTGGCGGTTAAACGCGCCACAAACGGCATGGCACAAATACTCAGCGGAATAATGGCGGCAGTGGTG  >  3/101959‑102441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑