Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	3	80,887	G→T	G105V (GGG→GTG)	unknown →	Recombinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	3	80,887	0	G	T	100.0%	211.1 / NA	78	G105V (GGG→GTG)	unknown	Recombinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (29/49);  total (29/49)

ATATACAACGTCGCTTCATTCAAAAATATGCTAAGGAACAAGGGCTTATACTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGGGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTCTGCCGGAGAATACTGTCGTGAGCTAGGTGAAAAAGTTTTTAATGGGCAGAAGAATTTGATTGAGCGCGGATTTCGTCAAGGTGGACCAGCTGGATTTGGGTTAAGACGCCTATTATTAAGTGCTGATG  >  3/80641‑81096                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                  atatACAACGTCGCTTCATTCAAAAATATGCTAAGGAACAAGGGCTTATACTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGAtt                                                                                                                                                                                                               >  1:585937/1‑251 (MQ=255)   ataCAACGTCGCTTCATTCAAAAATATGCTAAGGAACAAGGGCTTATACTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTaa                                                                                                                                                                                                             >  1:294120/1‑251 (MQ=255)   ataCAACGTCGCTTCATTCAAAAATATGCTAAGGAACAAGGGCTTATACTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTaa                                                                                                                                                                                                             >  1:261974/1‑251 (MQ=255)              cTTCATTCAAAAATATGCTAAGGAACAAGGGCTTATACTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCGGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTAc                                                                                                                                                                                                   <  1:160646/250‑1 (MQ=255)              cTTCATTCAAAAATATGCTAAGGAACAAGGGCTTATACTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTAc                                                                                                                                                                                                   <  1:605601/250‑1 (MQ=255)              cTTCATTCAAAAATATGCTAAGGAACAAGGGCTTATACTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTAc                                                                                                                                                                                                   <  1:300313/250‑1 (MQ=255)              cTTCATTCAAAAATATGCTAAGGAACAAGGGCTTATACTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTAc                                                                                                                                                                                                   <  1:744010/250‑1 (MQ=255)                   ttcaAAAATATGCTAAGGAACAAGGGCTTATACTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCa                                                                                                                                                                                             >  1:457715/1‑251 (MQ=255)                    tcaAAAATATGCTAAGGAACAAGGGCTTATACTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAg                                                                                                                                                                                            <  1:344605/251‑1 (MQ=255)                       aaaaTATGCTAAGGAACAAGGGCTTATACTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTAc                                                                                                                                                                                                   >  1:850108/1‑241 (MQ=255)                        aaaTATGCTAAGGAACAAGGGCTTATACTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAAcc                                                                                                                                                                                        <  1:241916/251‑1 (MQ=255)                           tatGCTAAGGAACAAGGGCTTATACTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGc                                                                                                                                                                                              <  1:730757/242‑1 (MQ=255)                            atGCTAAGGAACAAGGGCTTATACTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTAc                                                                                                                                                                                                   >  1:777094/1‑236 (MQ=255)                                    gAACACGGGCTTATACTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGCACCTTTTAAGGGaaa                                                                                                                                                                            >  1:464632/1‑251 (MQ=255)                                    gAACAAGGGCTTATACTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTAc                                                                                                                                                                                                   >  1:882974/1‑228 (MQ=255)                                    gAACAAGGGCTTATACTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTAc                                                                                                                                                                                                   >  1:136885/1‑228 (MQ=255)                                    gAACAAGGGCTTATACTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAAc                                                                                                                                                                                         <  1:667348/238‑1 (MQ=255)                                    gAACAAGGGCTTATACTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAg                                                                                                                                                                                 <  1:484521/246‑1 (MQ=255)                                    gAACAAGGGCTTATACTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGaaa                                                                                                                                                                            >  1:898734/1‑251 (MQ=255)                                           ggCTTATACTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACtg                                                                                                                                                                                                 <  1:680721/223‑1 (MQ=255)                                           ggCTTATACTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAg                                                                                                                                                                                            <  1:640873/228‑1 (MQ=255)                                           ggCTTATACTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTa                                                                                                                                                                                   <  1:625960/237‑1 (MQ=255)                                           ggCTTATACTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTa                                                                                                                                                                                   <  1:482660/237‑1 (MQ=255)                                           ggCTTATACTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACAtt                                                                                                                                                                     >  1:674090/1‑251 (MQ=255)                                              ttATACTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGt                                                                                                                                                                  <  1:182586/251‑1 (MQ=255)                                               tataCTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGtt                                                                                                                                                                 >  1:655353/1‑251 (MQ=255)                                                     cACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTAc                                                                                                                                                                                                   <  1:118333/211‑1 (MQ=255)                                                           ggAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTa                                                                                                                                                     >  1:191066/1‑251 (MQ=255)                                                                aCCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTAc                                                                                                                                                                                                   >  1:714482/1‑200 (MQ=255)                                                                aCCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCg                                                                                                                                                >  1:54510/1‑251 (MQ=255)                                                                 ccTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAg                                                                                                                                                  <  1:852361/248‑1 (MQ=255)                                                                        gAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAggg                                                                                                                                                                               <  1:146956/212‑1 (MQ=255)                                                                        gAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACg                                                                                                                                        >  1:508809/1‑251 (MQ=255)                                                                         aGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACg                                                                                                                                        <  1:663562/250‑1 (MQ=255)                                                                          gggAAGGAGTGGATTAAGCGTAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTAc                                                                                                                                                                                                   <  1:445907/190‑1 (MQ=255)                                                                           ggAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGt                                                                                                                                       <  1:7515/249‑1 (MQ=255)                                                                                gAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTc                                                                                                                                  <  1:818228/249‑1 (MQ=255)                                                                                    ggATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTgg                                                                                                                                                                   <  1:582871/212‑1 (MQ=255)                                                                                     gATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTCTGCCGga                                                                                                                           >  1:203859/1‑251 (MQ=255)                                                                                        tAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTCTGCCGGAGaa                                                                                                                        <  1:476611/251‑1 (MQ=255)                                                                                         aaGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGGAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTCTGCCGGAGAAt                                                                                                                       <  1:403762/251‑1 (MQ=255)                                                                                           gcgcAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTgg                                                                                                                                                                   <  1:689404/205‑1 (MQ=255)                                                                                            cgcAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTCTGCCGGAGAATAc                                                                                                                     <  1:879735/250‑1 (MQ=255)                                                                                                gAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAg                                                                                                                                                                                                           >  1:890594/1‑160 (MQ=255)                                                                                                gAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTAc                                                                                                                                                                                                   >  1:185397/1‑168 (MQ=255)                                                                                                 aaaaaCGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTCTGCCGGAGAATACTGt                                                                                                                  <  1:209106/248‑1 (MQ=255)                                                                                                  aaaaCGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTAc                                                                                                                                                                                                   <  1:413721/166‑1 (MQ=255)                                                                                                     aCGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTCTGCCGGAGAATACTGTCGTGAGc                                                                                                           <  1:764554/251‑1 (MQ=255)                                                                                                     aCGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTCTGCCGGAGAATACTGTCGTGAGc                                                                                                           <  1:528861/251‑1 (MQ=255)                                                                                                         ccTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTCTGCCGGAGAATACTGTCGTGAGCTAgg                                                                                                       <  1:282831/251‑1 (MQ=255)                                                                                                            cAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAAcc                                                                                                                                                                                        >  1:487558/1‑167 (MQ=255)                                                                                                              gTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGt                                                                                                                                       >  1:392833/1‑214 (MQ=255)                                                                                                              gTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTc                                                                                                                                  >  1:198100/1‑219 (MQ=255)                                                                                                              gTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTCTGCCGGAGAATACTGTCg                                                                                                                <  1:275004/237‑1 (MQ=255)                                                                                                              gTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTCTGCCGGAGAATACTGTCGTGAGCTAGGTGaaa                                                                                                  >  1:659287/1‑251 (MQ=255)                                                                                                                ttttATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTAc                                                                                                                                                                                                   <  1:341810/152‑1 (MQ=255)                                                                                                                        cTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTCTGCCGGAGAATACTGTCGTGAGCTAGGTGAAAAAGTTTTTaa                                                                                        >  1:203576/1‑251 (MQ=255)                                                                                                                          cATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCT‑AATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTgg                                                                                                                                                                   >  1:262854/1‑173 (MQ=255)                                                                                                                               aTTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTgg                                                                                                                                                                   <  1:301291/169‑1 (MQ=255)                                                                                                                                  ttGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTCTGCCGGAGAATACTGTCGTGAGCTAGGTGAAAAAGTTTTTAATGGGCagaag                                                                              <  1:873352/251‑1 (MQ=255)                                                                                                                                    gTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAg                                                                                                                                                                                            <  1:301282/139‑1 (MQ=255)                                                                                                                                       cAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGt                                                                                                                                       <  1:657243/189‑1 (MQ=255)                                                                                                                                         aTCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTCTGCCGGAGAATACTGTCGTGAGCTAGGTGAAAAAg                                                                                               <  1:638189/227‑1 (MQ=255)                                                                                                                                         aTCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTCTGCCGGAGAATACTGTCGTGAGCTAGGTGAAAAAGTTTTTAATGGGCAGAAGAATttga                                                                       >  1:52461/1‑251 (MQ=255)                                                                                                                                          tCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTCTGCCGGAGAATACTGTCGTGAGCTAGGTGAAAAAGTTTTTAATGGGCAGAAGAATttgat                                                                      <  1:8298/251‑1 (MQ=255)                                                                                                                                                                   ttCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTAc                                                                                                                                                                                                   >  1:349912/1‑101 (MQ=255)                                                                                                                                                                               aCATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAggg                                                                                                                                                                               <  1:615310/109‑1 (MQ=255)                                                                                                                                                                               aCATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGt                                                                                                                                       <  1:461207/149‑1 (MQ=255)                                                                                                                                                                                         aTGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTCTGCCGGAGAATACTGTCGTGAGCTAGGTGAAAAAGTTTTTAATGGGCAGAAGAATTTGATTGAGCGCGGATTTCGTCAAGGTGGACCAGCTGGATTTGGGTTAAGa                        <  1:799027/250‑1 (MQ=255)                                                                                                                                                                                                  cTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTCTGCCGGAGAATACTGTCGTGAGCTAGGTGAAAAAGTTTTTAATGGGCAGAAGAATTTGATTGAGCGCGGATTTCGTCAAGGTGGACCAGCTGGATTTGGGTTAAGACGCCtattat              <  1:376535/251‑1 (MQ=255)                                                                                                                                                                                                    ttCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTCTGCCGGAGAATACTGTCGTGAGCTAGGTGAAAAAGTTTTTAATGGGCAGAAGAATTTGATTGAGCGCGGATTTCGTCAAGGTGGACCAGCTGGATTTGGGTTAAGACGCCTAttatta            <  1:512202/251‑1 (MQ=255)                                                                                                                                                                                                    ttCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTCTGCCGGAGAATACTGTCGTGAGCTAGGTGAAAAAGTTTTTAATGGGCAGAAGAATTTGATTGAGCGCGGATTTCGTCAAGGTGGACCAGCTGGATTTGGGTTAAGACGCCTAttatta            <  1:186226/251‑1 (MQ=255)                                                                                                                                                                                                      cTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTCTGCCGGAGAATACTGTCGTGAGCTAGGTGAAAAAGTTTTTAATGGGCAGAAGAATTTGATTGAGCGCGGATTTCGTCAAGGTGGACCAGCTGGATTTGGGTTAAGACGCCTATTATTAAg          <  1:687745/251‑1 (MQ=255)                                                                                                                                                                                                      cTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTCTGCCGGAGAATACTGTCGTGAGCTAGGTGAAAAAGTTTTTAATGGGCAGAAGAATTTGATTGAGCGCGGATTTCGTCAAGGTGGACCAGCTGGATTTGGGTTAAGACGCCTATTATTAAg          <  1:740994/251‑1 (MQ=255)                                                                                                                                                                                                      cTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTCTGCCGGAGAATACTGTCGTGAGCTAGGTGAAAAAGTTTTTAATGGGCAGAAGAATTTGATTGAGCGCGGATTTCGTCAAGGTGGACCAGCTGGATTTGGGTTAAGACGCCTATTATTAAg          <  1:454831/251‑1 (MQ=255)                                                                                                                                                                                                        aaaTATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTCTGCCGGAGAATACTGTCGTGAGCTAGGTGAAAAAGTTTTTAATGGGCAGAAGAATTTGATTGAGCGCGGATTTCGTCAAGGTGGACCAGCTGGATTTGGGTTAAGACGCCTATTATTAAGTg        >  1:22620/1‑251 (MQ=255)                                                                                                                                                                                                               gatgaATCTGCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTCTGCCGGAGAATACTGTCGTGAGCTAGGTGAAAAAGTTTTTAATGGGCAGAAGAATTTGATTGAGCGCGGATTTCGTCAAGGTGGACCAGCTGGATTTGGGTTAAGACGCCTATTATTAAGTGCTGATg  >  1:232738/1‑250 (MQ=255)                                                                                                                                                                                                                        gCACATTATGAACAAATTTGTTCAAAAATGGTGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTCTGCCGGAGAATACTGTCGTg                                                                                                              <  1:667011/133‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                  ATATACAACGTCGCTTCATTCAAAAATATGCTAAGGAACAAGGGCTTATACTCACTAGGGAATACCTAGATGAGGGAAGGAGTGGATTAAGCGCAGAAAAACGTCCTCAGTTTTTATCACTCATTAATTTTGTACAATCCGGTAATGCTGATTTTTCACATATTCTTGTTTATGACATTAGCCGATGGGGGCGCTTTCTAAATATTGATGAATCTGCACATTATGAACAAATTTGTTCAAAAATGGGGATTAAAGTGCATTACTGTGCAGAACCTTTTAAGGGAAACGACATTGGTTCTCAAATTTTTAAAGCGGTAAAACGTTGGTCTGCCGGAGAATACTGTCGTGAGCTAGGTGAAAAAGTTTTTAATGGGCAGAAGAATTTGATTGAGCGCGGATTTCGTCAAGGTGGACCAGCTGGATTTGGGTTAAGACGCCTATTATTAAGTGCTGATG  >  3/80641‑81096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑