| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 397,951 | C→T | R230H (CGC→CAC) | unknown ← | Queuine tRNA‑ribosyltransferase (EC 2.4.2.29) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 397,951 | 0 | C | T | 98.8% | 209.3 / ‑5.9 | 81 | R230H (CGC→CAC) | unknown | Queuine tRNA‑ribosyltransferase (EC 2.4.2.29) |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (45/35); minor base G (1/0); total (46/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GTGTAACAGTCACAGTGCGGATCCGGCGGGCTGGTGTCGTCTTTGTATTTGGCATTACGGATTTTAACGATGCCGTCGGTGACGAATAAATGCCCATTACGCGCATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGCGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAGCGCAGGGACATTTCCATGGATTGCTTGGCATAATTAAACGTGGCGGGGTAGGGCGTACATT > 1/397711‑398197 | gtgtGACAGTCACAGTGCGGATCCGGCGGGCTGGTGTCGTCTTTGTATTTGGCATTACGGATTTTAACGATGCCGTCGGTGACGAATAAATGCCCATTACGCGCATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCatat > 1:707273/1‑250 (MQ=255) aaCAGTCACAGTGCGGATCCGGCGGGCTGGTGTCGTCTTTGTATTTGGCATTACGGATTTTAACGATGCCGTCGGTGACGAATAAATGCCCATTACGCGCATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATAtctt > 1:471075/1‑249 (MQ=255) gTGCGGATCCGGCGGGCTGGTGTCGTCTTTGTATTTGGCATTACGGATTTTAACGATGCCGTCGGTGACGAATAAATGCCCATTACGCGCATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCg > 1:71524/1‑250 (MQ=255) gTGCGGATCCGGCGGGCTGGTGTCGTCTTTGTATTTGGCATTACGGATTTTAACGATGCCGTCGGTGACGAATAAATGCCCATTACGCGCATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCg > 1:365683/1‑250 (MQ=255) tGCGGATCCGGCGGGCTGGTGTCGTCTTTGTATTTGGCATTACGGATTTTAACGATGCCGTCGGTGACGAATAAATGCCCATTACGCGCATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGcc < 1:542073/251‑1 (MQ=255) tCCGGCGGGCTGGTGTCGTCTTTGTATTTGGCATTACGGATTTTAACGATGCCGTCGGTGACGAATAAATGCCCATTACGCGCATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGc < 1:581171/251‑1 (MQ=255) cggGCTGGTGTCGTCTTTGTATTTGGCATTACGGATTTTAACGATGCCGTCGGTGACGAATAAATGCCCATTACGCGCATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAAc < 1:896851/251‑1 (MQ=255) cggGCTGGTGTCGTCTTTGTATTTGGCATTACGGATTTTAACGATGCCGTCGGTGACGAATAAATGCCCATTACGCGCATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAAc < 1:856590/251‑1 (MQ=255) cggGCTGGTGTCGTCTTTGTATTTGGCATTACGGATTTTAACGATGCCGTCGGTGACGAATAAATGCCCATTACGCGCATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAAc < 1:839550/251‑1 (MQ=255) cggGCTGGTGTCGTCTTTGTATTTGGCATTACGGATTTTAACGATGCCGTCGGTGACGAATAAATGCCCATTACGCGCATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAAc < 1:28407/251‑1 (MQ=255) ggCTGGTGTCGTCTTTGTATTTGGCATTACGGATTTTAACGATGCCGTCGGTGACGAATAAATGCCCATTACGCGCATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCg > 1:738344/1‑251 (MQ=255) tcTTTGTATTTGGCATTACGGATTTTAACGATGCCGTCGGTGACGAATAAATGCCCATTACGCGCATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATAtctt > 1:387813/1‑214 (MQ=255) tttGTATTTGGCATTACGGATTTTAACGATGCCGTCGGTGACGAATAAATGCCCATTACGCGCATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGc < 1:297878/251‑1 (MQ=255) tttGTATTTGGCATTACGGATTTTAACGATGCCGTCGGTGACGAATAAATGCCCATTACGCGCATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGc < 1:858401/251‑1 (MQ=255) tttGTATTTGGCATTACGGATTTTAACGATGCCGTCGGTGACGAATAAATGCCCATTACGCGCATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGc < 1:871681/251‑1 (MQ=255) gATTTTAACGATGCCGTCGGTGACGAATAAATGCCCATTACGCGCATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGccc > 1:594545/1‑208 (MQ=255) aTTTTAACGATGCCGTCGGTGACGAATAAATGCCCATTACGCGCATTGCGGGTTGGCATCACGCAATCGAACATATCGATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGAt < 1:312059/251‑1 (MQ=255) aTTTTAACGATGCCGTCGGTGACGAATAAATGCCCATTACGCGCATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGAt < 1:21808/251‑1 (MQ=255) gTCGGTGACGAATAAATGCCCATTACGCGCATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTg < 1:324979/235‑1 (MQ=255) gTCGGTGACGAATAAATGCCCATTACGCGCATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTg > 1:609483/1‑251 (MQ=255) aTAAATGCCCATTACGCGCATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTgcgc < 1:824632/251‑1 (MQ=255) tGCCCATTACGCGCATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAAtt > 1:610244/1‑250 (MQ=255) cccATTACGCGCATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAAttctt > 1:55347/1‑251 (MQ=255) cccATTACGCGCATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAAttctt > 1:427528/1‑251 (MQ=255) cATTACGCGCATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCttt > 1:110125/1‑163 (MQ=255) cATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCAc > 1:363388/1‑181 (MQ=255) cATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGcc < 1:252457/214‑1 (MQ=255) cATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCg > 1:407740/1‑251 (MQ=255) ggTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGAtt > 1:482449/1‑202 (MQ=255) ttGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGAt < 1:564218/250‑1 (MQ=255) cAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAg > 1:840122/1‑251 (MQ=255) aTCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGatat < 1:768399/182‑1 (MQ=255) aaCATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGtt > 1:667916/1‑251 (MQ=255) atatCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGc > 1:861861/1‑160 (MQ=255) tatCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTg > 1:509546/1‑176 (MQ=255) tatCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAgg < 1:92749/216‑1 (MQ=255) tatCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTttg > 1:756184/1‑251 (MQ=255) tCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTttggt > 1:770964/1‑249 (MQ=255) aTGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATAtcttc < 1:335171/116‑1 (MQ=255) ccGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTaa < 1:523908/251‑1 (MQ=255) cgACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTc < 1:741918/251‑1 (MQ=255) ccTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACg < 1:645799/251‑1 (MQ=255) tAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGc < 1:667753/251‑1 (MQ=255) aTCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGcc < 1:349217/153‑1 (MQ=255) ttCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAg > 1:531158/1‑251 (MQ=255) ttCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAg > 1:735908/1‑251 (MQ=255) tCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAg > 1:66596/1‑250 (MQ=255) cccATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTc > 1:880244/1‑212 (MQ=255) cccATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAGCGCAGGGa > 1:654107/1‑251 (MQ=255) cccATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAGCGCAGGGa > 1:37814/1‑251 (MQ=255) cccATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAGCGCAGGGa > 1:731337/1‑251 (MQ=255) ccATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTTGCCCACGGCTAAACCGCCCAc > 1:742621/1‑100 (MQ=255) ccATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGatat > 1:479410/1‑123 (MQ=255) ccATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGAt < 1:165838/178‑1 (MQ=255) ccATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAGCGCAGGGa > 1:806638/1‑250 (MQ=255) ccATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAGCGCAGGGAc < 1:96633/251‑1 (MQ=255) ccATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGGGGTGCATATCTTCTTTTGGGTTGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAGCGCAGGGag > 1:151155/1‑250 (MQ=255) aaaTAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAGCGCAGGGACAttt > 1:658782/1‑250 (MQ=255) aTAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAg > 1:264228/1‑132 (MQ=255) aTAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGGTAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAGCGCAGGGACATTTCCa > 1:445154/1‑251 (MQ=255) cgcgGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAGCGCAGGGACATTTCCATgg < 1:590782/250‑1 (MQ=255) cgGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAGCGCAGGGACATTTc < 1:40540/243‑1 (MQ=255) ttGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAGCGCAGGGACATTTCCATGGAt > 1:178061/1‑246 (MQ=255) gTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAGCGCAGGGACATTTCCATGGATTGCTTgg > 1:825037/1‑251 (MQ=255) aaTCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAGCGCAGGGACATTTCCATGGATTGCTTGGCATAATTa < 1:107425/250‑1 (MQ=255) aaTCTGCGGGCAGATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAGCGCAGGGACATTTCCATGGATTGCTTGGCATAATTa < 1:329468/250‑1 (MQ=255) gATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGcc > 1:846741/1‑93 (MQ=255) gATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCTAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAGCGCAGGGACATTTCCATGGATTGCTTGGCATAATTAAACGTGGCGGGGt > 1:738641/1‑251 (MQ=255) gATGTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAgc < 1:502453/203‑1 (MQ=255) gTACTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAGCGCAGGGACATTTCCATGGATTGCTTGGCATAATTAAACGTGGCGGGGGAgg > 1:115737/1‑251 (MQ=255) cTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAAc > 1:123521/1‑142 (MQ=255) cTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAGCGCAGGGACATTTCCATGGATTGCTTGGCAt > 1:730110/1‑227 (MQ=255) cTCCAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAGCGCAGGGACATTTCCATGGATTGCTTGGCATAATTAAACGTGGCGGGGTAGGGCg > 1:587187/1‑251 (MQ=255) ccAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAAc > 1:485037/1‑97 (MQ=255) ccAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCaa < 1:116705/114‑1 (MQ=255) ccAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAGCGCAGGGACATTTCCATGGAt > 1:807346/1‑215 (MQ=255) ccAAAATGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAGCGCAGGGACATTTCCATGGATTGCTTGGCATAATTAAACGTGGCGGGGTAGGGCGTa > 1:36835/1‑251 (MQ=255) aaaTGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCGCCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACGTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGGCCAGCGCAGGGACGTTTCCATGGATTGCTTGGCATAATTAAACGTGGCGGGGTAGGGCGTACAt < 1:99388/251‑1 (MQ=255) aaaTGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAGCGCAGGGACATTTCCATGGATTGCTTGGCATAATTAAACGTGGCGGGGTAGGGCGTACAt < 1:882535/251‑1 (MQ=255) aaaTGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAGCGCAGGGACATTTCCATGGATTGCTTGGCATAATTAAACGTGGCGGGGTAGGGCGTACAt < 1:99784/251‑1 (MQ=255) aaTGTGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAGCGCAGGGACATTTCCATGGATTGCTTGGCATAATTAAACGTGGCGGGGTAGGGCGTACAtt < 1:891350/251‑1 (MQ=255) | GTGTAACAGTCACAGTGCGGATCCGGCGGGCTGGTGTCGTCTTTGTATTTGGCATTACGGATTTTAACGATGCCGTCGGTGACGAATAAATGCCCATTACGCGCATTGCGGGTTGGCATCACGCAATCGAACATATCAATGCCGCGACGTACGCCTTCCACTAAATCTTCCGGTTTTCCTACACCCATTAAATAGCGCGGTTTGTCGGCGGGAATCTGCGGGCAGATGTACTCCAAAATGCGGTGCATATCTTCTTTTGGTTCGCCCACGGCTAAACCGCCCACCGCATAGCCGTCAAAGCCGATATTGATTAAGCCTTCCAGTGAAACTTTGCGCAATTCTTCAAAGGTTCCGCCTTGGATAATGCCGAACAGCGCGTTTTTGTTGCCTAATTCGTCAAAACGAGCGCGGCTGCGTTGCGCCCAGCGCAGGGACATTTCCATGGATTGCTTGGCATAATTAAACGTGGCGGGGTAGGGCGTACATT > 1/397711‑398197 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |